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The map label for this gene is nrdA

Identifier: 88608409

GI number: 88608409

Start: 336154

End: 337971

Strand: Reverse

Name: nrdA

Synonym: NSE_0411

Alternate gene names: 88608409

Gene position: 337971-336154 (Counterclockwise)

Preceding gene: 88608634

Following gene: 88608477

Centisome position: 39.34

GC content: 41.31

Gene sequence:

>1818_bases
ATGTTAGATCCTTCCCCTATTTTTCATCATGATCATGATGAAAAAAAAATCCATACACTGCATGCAAGGGACTCCTTTCT
TACAGATTTCGGAAAGGCAGTTCTCAAGGATAGATATTTGCAGAATGGCGAAGATTACCAGGGCCTTTTTGCAAGAGTAG
CTAAATATTACTCCGATGACCCGAAACATGCATGTAGACTGTACGACTATATGAGCAACTTATGGTTCATGCCTGCTACA
CCTATTCTCAGCAATGGTGGTACAAGTCGTGGACTCCCGGTTTCATGCTTCCTCAATGAAACAGAAGACTCATTGGAGGG
AATTGTCGGACTCTGGAATGAAAATGTATGGCTCGCGTCACATGGTGGCGGGGTCGGAAGTTACTGGGGTAACCTGCGCT
CAATAGGAGAAAAAGTAAAATGTAGTGGTAAGACTTCGGGAATTATTCCGTTTATTGTTGTACAAAATGCACTAACTCTT
GCAATATCACAGGGTTCACTTAGGCGTGGAAGTTCAGCCGTTTACTTACCAGTAAACCATCCGGAAATTATCGAATTTAT
AGACCTCAGAAAACCAACAGGTGGTGATCCAAATAGAAAAGCACTTAATTTGCATCATGCAGTCGTTATCAATGATGACT
TCATGAAAGCTGTCCTAGAGGATAAAGAGTGGGATCTTATCAGTCCAAAGGACGGTTCCGTGATAGCAACAGTCGGAGCA
AGGGGTATATGGATAAGAATACTTACAGCCCGTCTCGAAACCGGTGAGCCATATATTCTGTTTATTGACACAGTCCAGAG
ATTAAGGCCCGATGTATATAAAAACCTTAATCTCGATGTCAAAATGTCGAACCTATGCAGCGAGATAACACTTACAACGG
GTAAAGATCACCTCGATAATCATAGAACTGCTATTTGTTGTCTTTCTTCCCTAAATTTAGAAAAATTTGATGAATGGGAG
CATAATGAGCAATTTGTACGGGACGTGATGCATTTCTTGGACAACGTGCTGGAGGACTTTATTAGAAAAGCACCTGATTG
CATGCACAGAGCAAGATATTCTGCCATGCGAGAAAGAAGTATAGGCTTGGGTGTAATGGGGTTTCACTCCTTCCTGCAAA
GTAAGGGTATTCCGTTTGAATCGGCAATTGCGAAAAGTGTTAACCTAAAAATTTTTAAAAAAATTAAACACGAAGCAGAT
AAGATTTCGAAAGAAATGGCACTAGAGAGGGGTCCTTGTAAGGATGCAGTGGATTGCGATACTATGGAGCGGTTTGTCCA
TAAGATCGCTGTTGCGCCAACAGCATCCATCTCGATCATTGCGGGTAATACTTCACCAGGTATTGAACCATACGCAGCAA
ATGTTTTCACACAAAAAACTTTAAGTGGATCTTTCGTTGTCAAGAATAAGCATCTCGCTGCCCTCCTAGAACGCAAGGGA
AAAAATACGGACTGTGTATGGTCCTCTATTATTTCTAATGAGGGCTCTGTACAGCACTTAGACTTTCTCGATGAGAATGA
AAAGATGGTTTTTCGAACAGCATACGAAATGGACCAACGATGGGTTATCGAACTCGCCGCTGATCGTACACCCTATATAT
GCCAATCCCAGTCAGTAAATCTCTTTCTACACTCTAACATACACAAAAAAGAACTAAGTAATCTACACTTTCTCGGATGG
AAAAAAGGCCTAAAAAGCCTTTACTATTGCCGGTCAAAATCCATACAGCGTGCATATAAAATATCACAGGAATACAAACA
TAAAAGTGATACTCCTTCTCCTGTTGGTGAATCAGATGATTGCATCGCGTGCCAATAG

Upstream 100 bases:

>100_bases
CTGTTGATCTTTCTACTATATATAGACTTTATAGCCATGCGTGTCGTCACGGCACTGGTCCAGGGAAATGATATCATCTC
GTATGAACAAAGAAGCTAAA

Downstream 100 bases:

>100_bases
CTTACGCCATGGGTCATATCCATAAGAAGAACCTCTCAAGGCTAGACCTTCCTTTTACCCTGAGGTGAGCTACTCTTTCG
CCATCTGCCTATAGAAGGAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MLDPSPIFHHDHDEKKIHTLHARDSFLTDFGKAVLKDRYLQNGEDYQGLFARVAKYYSDDPKHACRLYDYMSNLWFMPAT
PILSNGGTSRGLPVSCFLNETEDSLEGIVGLWNENVWLASHGGGVGSYWGNLRSIGEKVKCSGKTSGIIPFIVVQNALTL
AISQGSLRRGSSAVYLPVNHPEIIEFIDLRKPTGGDPNRKALNLHHAVVINDDFMKAVLEDKEWDLISPKDGSVIATVGA
RGIWIRILTARLETGEPYILFIDTVQRLRPDVYKNLNLDVKMSNLCSEITLTTGKDHLDNHRTAICCLSSLNLEKFDEWE
HNEQFVRDVMHFLDNVLEDFIRKAPDCMHRARYSAMRERSIGLGVMGFHSFLQSKGIPFESAIAKSVNLKIFKKIKHEAD
KISKEMALERGPCKDAVDCDTMERFVHKIAVAPTASISIIAGNTSPGIEPYAANVFTQKTLSGSFVVKNKHLAALLERKG
KNTDCVWSSIISNEGSVQHLDFLDENEKMVFRTAYEMDQRWVIELAADRTPYICQSQSVNLFLHSNIHKKELSNLHFLGW
KKGLKSLYYCRSKSIQRAYKISQEYKHKSDTPSPVGESDDCIACQ

Sequences:

>Translated_605_residues
MLDPSPIFHHDHDEKKIHTLHARDSFLTDFGKAVLKDRYLQNGEDYQGLFARVAKYYSDDPKHACRLYDYMSNLWFMPAT
PILSNGGTSRGLPVSCFLNETEDSLEGIVGLWNENVWLASHGGGVGSYWGNLRSIGEKVKCSGKTSGIIPFIVVQNALTL
AISQGSLRRGSSAVYLPVNHPEIIEFIDLRKPTGGDPNRKALNLHHAVVINDDFMKAVLEDKEWDLISPKDGSVIATVGA
RGIWIRILTARLETGEPYILFIDTVQRLRPDVYKNLNLDVKMSNLCSEITLTTGKDHLDNHRTAICCLSSLNLEKFDEWE
HNEQFVRDVMHFLDNVLEDFIRKAPDCMHRARYSAMRERSIGLGVMGFHSFLQSKGIPFESAIAKSVNLKIFKKIKHEAD
KISKEMALERGPCKDAVDCDTMERFVHKIAVAPTASISIIAGNTSPGIEPYAANVFTQKTLSGSFVVKNKHLAALLERKG
KNTDCVWSSIISNEGSVQHLDFLDENEKMVFRTAYEMDQRWVIELAADRTPYICQSQSVNLFLHSNIHKKELSNLHFLGW
KKGLKSLYYCRSKSIQRAYKISQEYKHKSDTPSPVGESDDCIACQ
>Mature_605_residues
MLDPSPIFHHDHDEKKIHTLHARDSFLTDFGKAVLKDRYLQNGEDYQGLFARVAKYYSDDPKHACRLYDYMSNLWFMPAT
PILSNGGTSRGLPVSCFLNETEDSLEGIVGLWNENVWLASHGGGVGSYWGNLRSIGEKVKCSGKTSGIIPFIVVQNALTL
AISQGSLRRGSSAVYLPVNHPEIIEFIDLRKPTGGDPNRKALNLHHAVVINDDFMKAVLEDKEWDLISPKDGSVIATVGA
RGIWIRILTARLETGEPYILFIDTVQRLRPDVYKNLNLDVKMSNLCSEITLTTGKDHLDNHRTAICCLSSLNLEKFDEWE
HNEQFVRDVMHFLDNVLEDFIRKAPDCMHRARYSAMRERSIGLGVMGFHSFLQSKGIPFESAIAKSVNLKIFKKIKHEAD
KISKEMALERGPCKDAVDCDTMERFVHKIAVAPTASISIIAGNTSPGIEPYAANVFTQKTLSGSFVVKNKHLAALLERKG
KNTDCVWSSIISNEGSVQHLDFLDENEKMVFRTAYEMDQRWVIELAADRTPYICQSQSVNLFLHSNIHKKELSNLHFLGW
KKGLKSLYYCRSKSIQRAYKISQEYKHKSDTPSPVGESDDCIACQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=622, Percent_Identity=31.6720257234727, Blast_Score=261, Evalue=2e-69,
Organism=Escherichia coli, GI1789030, Length=635, Percent_Identity=27.0866141732283, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI2367133, Length=466, Percent_Identity=26.8240343347639, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17555242, Length=669, Percent_Identity=32.1375186846039, Blast_Score=287, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320914, Length=648, Percent_Identity=31.6358024691358, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6322123, Length=659, Percent_Identity=31.2594840667678, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17352481, Length=649, Percent_Identity=30.2003081664099, Blast_Score=244, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 68260; Mature: 68260

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPSPIFHHDHDEKKIHTLHARDSFLTDFGKAVLKDRYLQNGEDYQGLFARVAKYYSDD
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
PKHACRLYDYMSNLWFMPATPILSNGGTSRGLPVSCFLNETEDSLEGIVGLWNENVWLAS
HHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEE
HGGGVGSYWGNLRSIGEKVKCSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAVYLPVNH
CCCCCHHHHHHHHHHCHHEECCCCCCCCCHHEECCCCEEEEEECCCCCCCCCEEEEECCC
PEIIEFIDLRKPTGGDPNRKALNLHHAVVINDDFMKAVLEDKEWDLISPKDGSVIATVGA
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHCCCCCCEECCCCCCEEEECCC
RGIWIRILTARLETGEPYILFIDTVQRLRPDVYKNLNLDVKMSNLCSEITLTTGKDHLDN
CCEEEEEEEEHHCCCCCEEEEECCHHHHCHHHHHCCCCEEEHHHHHHHEEEECCCHHHCC
HRTAICCLSSLNLEKFDEWEHNEQFVRDVMHFLDNVLEDFIRKAPDCMHRARYSAMRERS
CCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
IGLGVMGFHSFLQSKGIPFESAIAKSVNLKIFKKIKHEADKISKEMALERGPCKDAVDCD
CCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TMERFVHKIAVAPTASISIIAGNTSPGIEPYAANVFTQKTLSGSFVVKNKHLAALLERKG
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCC
KNTDCVWSSIISNEGSVQHLDFLDENEKMVFRTAYEMDQRWVIELAADRTPYICQSQSVN
CCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEHHHCCCEEEEEEECCCCCCEECCCCEE
LFLHSNIHKKELSNLHFLGWKKGLKSLYYCRSKSIQRAYKISQEYKHKSDTPSPVGESDD
EEEECCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CIACQ
CEECC
>Mature Secondary Structure
MLDPSPIFHHDHDEKKIHTLHARDSFLTDFGKAVLKDRYLQNGEDYQGLFARVAKYYSDD
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
PKHACRLYDYMSNLWFMPATPILSNGGTSRGLPVSCFLNETEDSLEGIVGLWNENVWLAS
HHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEE
HGGGVGSYWGNLRSIGEKVKCSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAVYLPVNH
CCCCCHHHHHHHHHHCHHEECCCCCCCCCHHEECCCCEEEEEECCCCCCCCCEEEEECCC
PEIIEFIDLRKPTGGDPNRKALNLHHAVVINDDFMKAVLEDKEWDLISPKDGSVIATVGA
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEECHHHHHHHHCCCCCCEECCCCCCEEEECCC
RGIWIRILTARLETGEPYILFIDTVQRLRPDVYKNLNLDVKMSNLCSEITLTTGKDHLDN
CCEEEEEEEEHHCCCCCEEEEECCHHHHCHHHHHCCCCEEEHHHHHHHEEEECCCHHHCC
HRTAICCLSSLNLEKFDEWEHNEQFVRDVMHFLDNVLEDFIRKAPDCMHRARYSAMRERS
CCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHC
IGLGVMGFHSFLQSKGIPFESAIAKSVNLKIFKKIKHEADKISKEMALERGPCKDAVDCD
CCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
TMERFVHKIAVAPTASISIIAGNTSPGIEPYAANVFTQKTLSGSFVVKNKHLAALLERKG
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHCC
KNTDCVWSSIISNEGSVQHLDFLDENEKMVFRTAYEMDQRWVIELAADRTPYICQSQSVN
CCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEHHHCCCEEEEEEECCCCCCEECCCCEE
LFLHSNIHKKELSNLHFLGWKKGLKSLYYCRSKSIQRAYKISQEYKHKSDTPSPVGESDD
EEEECCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CIACQ
CEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]