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The map label for this gene is rnhA

Identifier: 88608239

GI number: 88608239

Start: 584101

End: 584556

Strand: Reverse

Name: rnhA

Synonym: NSE_0667

Alternate gene names: 88608239

Gene position: 584556-584101 (Counterclockwise)

Preceding gene: 88608309

Following gene: 88608352

Centisome position: 68.05

GC content: 41.23

Gene sequence:

>456_bases
ATGGAAGAATACGTGATATACACCGACGGTGCATGTTTAGGTAATCCAGGTCCTGGTGGTTGGGCTGCAGTGATAATTCA
GAGAGGTACCAACGAAAAGATAATTTCCGGGAGAGAGGCAGATAGTACAAATAACAAAATGGAGTTGCTTGCAGTAATAA
AAGCTCTGGAGTCGTTTGAACAAAAAGGAAAAAGAGTCACTGTATACACCGATAGTACTTATGTCTACAAAGGTATCACT
GCTTGGATAGAGAGTTGGATGAAAAATAAGTGGCGTAACTCATCAGGAGGAGCTGTAAAAAACAAGGAAATGTGGGTCAG
GCTACATGGAATTGCAGCCGCACATACTGTACGATGGTTGTGGGTTAAGGCTCATAATGGTGATCATTATAACGAAATTG
TTGATAGAGTAGCACGAAAAGAAGCTGCCTCTTTTACGAATTGCGAACCTGAATAA

Upstream 100 bases:

>100_bases
TGGTGTGGGTGAGTTATCAAGTTATGCACATGAAAAAGGATAATTCGATATCATTGTTCATTCCGAGTTGATAAGATATT
GACGATCTAGGGTTTTAAGT

Downstream 100 bases:

>100_bases
AGTTTTTGTTGTGTTAAGTGTGTCGTTGGTAATTAATTTCTTCTAATGTAGAGACTACGGCGCCCTATTGGGACTTTCCA
GTGTTCAGAATTGCAGTCTG

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MEEYVIYTDGACLGNPGPGGWAAVIIQRGTNEKIISGREADSTNNKMELLAVIKALESFEQKGKRVTVYTDSTYVYKGIT
AWIESWMKNKWRNSSGGAVKNKEMWVRLHGIAAAHTVRWLWVKAHNGDHYNEIVDRVARKEAASFTNCEPE

Sequences:

>Translated_151_residues
MEEYVIYTDGACLGNPGPGGWAAVIIQRGTNEKIISGREADSTNNKMELLAVIKALESFEQKGKRVTVYTDSTYVYKGIT
AWIESWMKNKWRNSSGGAVKNKEMWVRLHGIAAAHTVRWLWVKAHNGDHYNEIVDRVARKEAASFTNCEPE
>Mature_151_residues
MEEYVIYTDGACLGNPGPGGWAAVIIQRGTNEKIISGREADSTNNKMELLAVIKALESFEQKGKRVTVYTDSTYVYKGIT
AWIESWMKNKWRNSSGGAVKNKEMWVRLHGIAAAHTVRWLWVKAHNGDHYNEIVDRVARKEAASFTNCEPE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=144, Percent_Identity=34.7222222222222, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1786408, Length=146, Percent_Identity=43.8356164383562, Blast_Score=127, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI115532542, Length=145, Percent_Identity=32.4137931034483, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=31.5068493150685, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=31.5068493150685, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_NEOSM (Q2GDA1)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506545.1
- ProteinModelPortal:   Q2GDA1
- STRING:   Q2GDA1
- GeneID:   3931461
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0667
- TIGR:   NSE_0667
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   SKVVIYT
- PhylomeDB:   Q2GDA1
- ProtClustDB:   CLSK2527792
- BioCyc:   NSEN222891:NSE_0667-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17019; Mature: 17019

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEYVIYTDGACLGNPGPGGWAAVIIQRGTNEKIISGREADSTNNKMELLAVIKALESFE
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
QKGKRVTVYTDSTYVYKGITAWIESWMKNKWRNSSGGAVKNKEMWVRLHGIAAAHTVRWL
HCCCEEEEEECCEEEEHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECHHHHCEEEEE
WVKAHNGDHYNEIVDRVARKEAASFTNCEPE
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEEYVIYTDGACLGNPGPGGWAAVIIQRGTNEKIISGREADSTNNKMELLAVIKALESFE
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHH
QKGKRVTVYTDSTYVYKGITAWIESWMKNKWRNSSGGAVKNKEMWVRLHGIAAAHTVRWL
HCCCEEEEEECCEEEEHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECHHHHCEEEEE
WVKAHNGDHYNEIVDRVARKEAASFTNCEPE
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA