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The map label for this gene is hemB

Identifier: 88608217

GI number: 88608217

Start: 321045

End: 322010

Strand: Direct

Name: hemB

Synonym: NSE_0394

Alternate gene names: 88608217

Gene position: 321045-322010 (Clockwise)

Preceding gene: 88608268

Following gene: 88608811

Centisome position: 37.37

GC content: 41.72

Gene sequence:

>966_bases
ATGCAATCTGGTTCTTTTGTCCGTCTTAGAAGGACGCGTAGAAATAAAATTATCCGAGACTTAGTTGCTGAAACCACACT
CTCCGCTTCGCAGCTCATATTACCATTGTTTGTCACAAATGGTTCAAATATTGAGTTGCAAGTGAATGAAATGCCGGGTG
TGTATGCACATTCTATCGATATACTAAAAAAAATAGTAGAGAGCGCAATAACAGTTGGTGTGAGGAGTATCATGCTTTTT
CCCCGGGATCCCGGGAAAAGAAGCTTTGAAGCGGAGGAAGCCTATAATCCGGAGAATTTGATCTGCCGTGCAGTGAGAGC
TCTTAAAAGGGATTTCGGAGGTGACCTTGTTATTATTACTGATATTGCGGCCGATCCTTATACAATTGATGGCCACGATG
GATTTTATAGAGATGGAAAAATTCTTAATGACGAAACATTAGAACTCTTGATAAAGCAAGCTTTGGTTCAGGCTGCAGCT
GGAAGCGATATGCTTGCCCCATCGGATATGATGGATGGAAGAATTTTTGCAATCAGAAATGCTCTCAATCTTGCTGAATT
TGATGAGGTCCCGATTGTATCGTATTCTGCTAAATATGCTTCCAATTTCTATGCACCTTTCCGAGACGCATTGGGCTCTA
CGAATATCATCCTGGACAAACGATCCTATCAAATGGATTATAGAAATTCAAAAGAAGCCATGCGAGAGATCGAAGTTGAT
ATTAACGAGGGTGCTGACATCGTAATGATCAAACCAGCCAGTTTCTACCTTGACATAATAAAAATGGCAACGGATCGGTT
TTGTGTGCCAATCTTTGCATACCAGGTAAGTGGTGAGTACAAAATGTTGGTTCAATATGGTGGTCTTGAAGTGTTATTGG
AGTCCCTAATTGCAATAAGAAGAGCGGGCGCAGCTGGAATAGTCACGTATGCTGCTATCGAGGTTGCAAAGTACCTGCCA
TCTTAG

Upstream 100 bases:

>100_bases
AGGAGAATAGTGCCTTTACTTGGTCGTACAATACTCGTACTAATTTGAGCCTCATTTCGTATGATGATAGTATTCCACGT
GTAAATTAGAGATATTAGGA

Downstream 100 bases:

>100_bases
GCATCTTTTTGGTTTATTTAGTTCCAATTATTTTGGAACCAAAGAGTAAATTGATCAGAAGCTATTGTATGTGAAAGCCT
TCCTATGACTTATCTCTCCT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQSGSFVRLRRTRRNKIIRDLVAETTLSASQLILPLFVTNGSNIELQVNEMPGVYAHSIDILKKIVESAITVGVRSIMLF
PRDPGKRSFEAEEAYNPENLICRAVRALKRDFGGDLVIITDIAADPYTIDGHDGFYRDGKILNDETLELLIKQALVQAAA
GSDMLAPSDMMDGRIFAIRNALNLAEFDEVPIVSYSAKYASNFYAPFRDALGSTNIILDKRSYQMDYRNSKEAMREIEVD
INEGADIVMIKPASFYLDIIKMATDRFCVPIFAYQVSGEYKMLVQYGGLEVLLESLIAIRRAGAAGIVTYAAIEVAKYLP
S

Sequences:

>Translated_321_residues
MQSGSFVRLRRTRRNKIIRDLVAETTLSASQLILPLFVTNGSNIELQVNEMPGVYAHSIDILKKIVESAITVGVRSIMLF
PRDPGKRSFEAEEAYNPENLICRAVRALKRDFGGDLVIITDIAADPYTIDGHDGFYRDGKILNDETLELLIKQALVQAAA
GSDMLAPSDMMDGRIFAIRNALNLAEFDEVPIVSYSAKYASNFYAPFRDALGSTNIILDKRSYQMDYRNSKEAMREIEVD
INEGADIVMIKPASFYLDIIKMATDRFCVPIFAYQVSGEYKMLVQYGGLEVLLESLIAIRRAGAAGIVTYAAIEVAKYLP
S
>Mature_321_residues
MQSGSFVRLRRTRRNKIIRDLVAETTLSASQLILPLFVTNGSNIELQVNEMPGVYAHSIDILKKIVESAITVGVRSIMLF
PRDPGKRSFEAEEAYNPENLICRAVRALKRDFGGDLVIITDIAADPYTIDGHDGFYRDGKILNDETLELLIKQALVQAAA
GSDMLAPSDMMDGRIFAIRNALNLAEFDEVPIVSYSAKYASNFYAPFRDALGSTNIILDKRSYQMDYRNSKEAMREIEVD
INEGADIVMIKPASFYLDIIKMATDRFCVPIFAYQVSGEYKMLVQYGGLEVLLESLIAIRRAGAAGIVTYAAIEVAKYLP
S

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=334, Percent_Identity=35.3293413173653, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=41.25, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6321398, Length=319, Percent_Identity=34.4827586206897, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21358291, Length=312, Percent_Identity=33.6538461538462, Blast_Score=176, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35748; Mature: 35748

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGSFVRLRRTRRNKIIRDLVAETTLSASQLILPLFVTNGSNIELQVNEMPGVYAHSID
CCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCEEEEEEECCCCHHHHHHH
ILKKIVESAITVGVRSIMLFPRDPGKRSFEAEEAYNPENLICRAVRALKRDFGGDLVIIT
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
DIAADPYTIDGHDGFYRDGKILNDETLELLIKQALVQAAAGSDMLAPSDMMDGRIFAIRN
ECCCCCEEECCCCCEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEHH
ALNLAEFDEVPIVSYSAKYASNFYAPFRDALGSTNIILDKRSYQMDYRNSKEAMREIEVD
HCCHHHCCCCCEEEECHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHEEE
INEGADIVMIKPASFYLDIIKMATDRFCVPIFAYQVSGEYKMLVQYGGLEVLLESLIAIR
ECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHH
RAGAAGIVTYAAIEVAKYLPS
HCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSGSFVRLRRTRRNKIIRDLVAETTLSASQLILPLFVTNGSNIELQVNEMPGVYAHSID
CCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCEEEEEEECCCCHHHHHHH
ILKKIVESAITVGVRSIMLFPRDPGKRSFEAEEAYNPENLICRAVRALKRDFGGDLVIIT
HHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
DIAADPYTIDGHDGFYRDGKILNDETLELLIKQALVQAAAGSDMLAPSDMMDGRIFAIRN
ECCCCCEEECCCCCEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEHH
ALNLAEFDEVPIVSYSAKYASNFYAPFRDALGSTNIILDKRSYQMDYRNSKEAMREIEVD
HCCHHHCCCCCEEEECHHHHHCCCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHEEE
INEGADIVMIKPASFYLDIIKMATDRFCVPIFAYQVSGEYKMLVQYGGLEVLLESLIAIR
ECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHH
RAGAAGIVTYAAIEVAKYLPS
HCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]