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The map label for this gene is def

Identifier: 88608268

GI number: 88608268

Start: 320139

End: 320699

Strand: Direct

Name: def

Synonym: NSE_0392

Alternate gene names: 88608268

Gene position: 320139-320699 (Clockwise)

Preceding gene: 88608876

Following gene: 88608217

Centisome position: 37.27

GC content: 44.21

Gene sequence:

>561_bases
ATGGCGTTACTTGCACTAATCATAGAGCCTGACCCACTGTTGCATGAAGTTTCAGAAGCTGTGGCTGGTTTATCAGATGA
AAAAAGGGTGTTTCTCGATGATATGCTGGAGACGATGTACCACTGTGGGGGTATAGGGCTGGCTGCAGTTCAAGTGGGTG
TACTTGAGAGAATTATTGTTGTTGATGTTCCTGTGGGTAAAGAGTGGCATTCAAGTCCTCTTAATCATGTAGGATACAAA
AGCTCAGGAGGTCCGTACTATTTTGTTAACCCCGAAATCATAGAATTCTCACAGAACTTGGTTCCTGCCGATGAAGGATG
TCTTTCACTTCCAGAACAGCATTACGAGATAATACGCCCGGATGCGGTGGTAGTTAAATATCTGAACTATGATGGAGAAG
AGTGCTTACTCAAGGCAAATGGTTGGCTGGCGCGCTGTATACAACATGAGATGGATCACCTGAATGGAAGATTATACGTG
TCGCATCTGTCGAAACTAAAGCATGATCTCGCAATAAAGAAGGCAGCTGAAATAAAGAAACGGCACTCTGAAGCAGACTG
A

Upstream 100 bases:

>100_bases
TGTGAATTTTTGATATCATTAAGTGTGCGGTAGGATAGCTTGCGAGAAAGTTTTTGTTCTGCTAGAGTCTAGAGCTTCTT
ATTTTTTACTCTGTTTTTCG

Downstream 100 bases:

>100_bases
CACCACGCCCAGGCCTTGCCGATGCAGGACTGAAAGGACAATAAAGTAGGCAAGAGAACGGTGGGTGGTAACGGGCTCGA
ACCGCTGACCTCCTCGGTGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MALLALIIEPDPLLHEVSEAVAGLSDEKRVFLDDMLETMYHCGGIGLAAVQVGVLERIIVVDVPVGKEWHSSPLNHVGYK
SSGGPYYFVNPEIIEFSQNLVPADEGCLSLPEQHYEIIRPDAVVVKYLNYDGEECLLKANGWLARCIQHEMDHLNGRLYV
SHLSKLKHDLAIKKAAEIKKRHSEAD

Sequences:

>Translated_186_residues
MALLALIIEPDPLLHEVSEAVAGLSDEKRVFLDDMLETMYHCGGIGLAAVQVGVLERIIVVDVPVGKEWHSSPLNHVGYK
SSGGPYYFVNPEIIEFSQNLVPADEGCLSLPEQHYEIIRPDAVVVKYLNYDGEECLLKANGWLARCIQHEMDHLNGRLYV
SHLSKLKHDLAIKKAAEIKKRHSEAD
>Mature_185_residues
ALLALIIEPDPLLHEVSEAVAGLSDEKRVFLDDMLETMYHCGGIGLAAVQVGVLERIIVVDVPVGKEWHSSPLNHVGYKS
SGGPYYFVNPEIIEFSQNLVPADEGCLSLPEQHYEIIRPDAVVVKYLNYDGEECLLKANGWLARCIQHEMDHLNGRLYVS
HLSKLKHDLAIKKAAEIKKRHSEAD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=31.2101910828025, Blast_Score=71, Evalue=6e-13,
Organism=Escherichia coli, GI1789682, Length=185, Percent_Identity=37.2972972972973, Blast_Score=117, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=28.9308176100629, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_NEOSM (Q2GE16)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506280.1
- ProteinModelPortal:   Q2GE16
- SMR:   Q2GE16
- STRING:   Q2GE16
- GeneID:   3931718
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0392
- TIGR:   NSE_0392
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   KANGWLA
- PhylomeDB:   Q2GE16
- ProtClustDB:   CLSK2527811
- BioCyc:   NSEN222891:NSE_0392-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20767; Mature: 20636

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: ACT_SITE 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLALIIEPDPLLHEVSEAVAGLSDEKRVFLDDMLETMYHCGGIGLAAVQVGVLERIIV
CCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEE
VDVPVGKEWHSSPLNHVGYKSSGGPYYFVNPEIIEFSQNLVPADEGCLSLPEQHYEIIRP
EECCCCCCHHCCCHHHCCCCCCCCCEEEECCHHEECCCCCCCCCCCHHCCCHHHHHHHCC
DAVVVKYLNYDGEECLLKANGWLARCIQHEMDHLNGRLYVSHLSKLKHDLAIKKAAEIKK
CHHEEHEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHH
RHSEAD
HCCCCC
>Mature Secondary Structure 
ALLALIIEPDPLLHEVSEAVAGLSDEKRVFLDDMLETMYHCGGIGLAAVQVGVLERIIV
CEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEE
VDVPVGKEWHSSPLNHVGYKSSGGPYYFVNPEIIEFSQNLVPADEGCLSLPEQHYEIIRP
EECCCCCCHHCCCHHHCCCCCCCCCEEEECCHHEECCCCCCCCCCCHHCCCHHHHHHHCC
DAVVVKYLNYDGEECLLKANGWLARCIQHEMDHLNGRLYVSHLSKLKHDLAIKKAAEIKK
CHHEEHEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHH
RHSEAD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA