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The map label for this gene is yjfH [C]

Identifier: 88608166

GI number: 88608166

Start: 223091

End: 223822

Strand: Reverse

Name: yjfH [C]

Synonym: NSE_0262

Alternate gene names: 88608166

Gene position: 223822-223091 (Counterclockwise)

Preceding gene: 88608015

Following gene: 88608589

Centisome position: 26.06

GC content: 40.03

Gene sequence:

>732_bases
GTGCAACAGGAGAAGGTCGTAATATTTGGGAAACATGCTGCCGTAGCAGCGATAAAAAATCCTGAGAGAAATATCTTATC
TATCTTCATCGCTAGGGAGAAAAAGGCTGTTTACTTACCATTGCTCGAACGATCTAGATTGCCAAATATTTCTTTTTGTG
AAAATACGCAAATTGCGAAGATTTGTGGAAGGGATGCGCTTCACCAAGGAATAGCAGTGGTAACCGAAAAACTCTCACAA
CCCGAGCTAAGAGACGTACTAAAAAGCACCAGCAAGAGGTCCCTGGTTATAATACTGGATAGTATAACTGATCCCTTTAA
TATCGGTAACATACTGAGATCATCCAAGGCTTTTGGTGTAGATGCACTTGTAGTTAAGGAACGTCATTCACCGAGTGAAA
CTTCTGTTATCGCAAAAACAGCTAGCGGTGCCTTAGAGAGCATACCAGTTTGCACAATAGCAAACCTTACAAATGCAGTA
AAGATTCTACAAGAAAACGGCTATTTTTGTTATGCACTGGATGGAGGAGCAAGGTCTAAGTTAACAGAAATAAGATTCCC
GAATAAAGTTGCCTTTGTACTCGGCTCAGAAGGAAAAGGTATCTCACATCTCCTAAAAACCAGATGTGATCAAACCCTTT
CTATACCGATGTACAGCGATAAAAATTTCGATAGTATTAATGTTTCTAACGCTGCTGCTATAGCAATGTTCAGATTCTAC
AGTGCCGAATAA

Upstream 100 bases:

>100_bases
CTTTTGACCACTCAGCCATCCCTCCCAACACAACGCTGTAACACGCAAAGTTTTTTTGCTAAGATCAGTCGTCGTGATAT
TCTAACACTCTGTCGGGCAA

Downstream 100 bases:

>100_bases
ATTCTTCACAGCATACACTAGACTCGAAAATTTTGTTCATTCGAAGCACTATCATATGTCGCGTTCGATGCTAGGAAGGA
TTCAATCAAAATTAAGTTCT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MQQEKVVIFGKHAAVAAIKNPERNILSIFIAREKKAVYLPLLERSRLPNISFCENTQIAKICGRDALHQGIAVVTEKLSQ
PELRDVLKSTSKRSLVIILDSITDPFNIGNILRSSKAFGVDALVVKERHSPSETSVIAKTASGALESIPVCTIANLTNAV
KILQENGYFCYALDGGARSKLTEIRFPNKVAFVLGSEGKGISHLLKTRCDQTLSIPMYSDKNFDSINVSNAAAIAMFRFY
SAE

Sequences:

>Translated_243_residues
MQQEKVVIFGKHAAVAAIKNPERNILSIFIAREKKAVYLPLLERSRLPNISFCENTQIAKICGRDALHQGIAVVTEKLSQ
PELRDVLKSTSKRSLVIILDSITDPFNIGNILRSSKAFGVDALVVKERHSPSETSVIAKTASGALESIPVCTIANLTNAV
KILQENGYFCYALDGGARSKLTEIRFPNKVAFVLGSEGKGISHLLKTRCDQTLSIPMYSDKNFDSINVSNAAAIAMFRFY
SAE
>Mature_243_residues
MQQEKVVIFGKHAAVAAIKNPERNILSIFIAREKKAVYLPLLERSRLPNISFCENTQIAKICGRDALHQGIAVVTEKLSQ
PELRDVLKSTSKRSLVIILDSITDPFNIGNILRSSKAFGVDALVVKERHSPSETSVIAKTASGALESIPVCTIANLTNAV
KILQENGYFCYALDGGARSKLTEIRFPNKVAFVLGSEGKGISHLLKTRCDQTLSIPMYSDKNFDSINVSNAAAIAMFRFY
SAE

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=257, Percent_Identity=24.9027237354086, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1790623, Length=236, Percent_Identity=31.7796610169492, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788935, Length=242, Percent_Identity=21.900826446281, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=250, Percent_Identity=31.6, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=32.7272727272727, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26629; Mature: 26629

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQEKVVIFGKHAAVAAIKNPERNILSIFIAREKKAVYLPLLERSRLPNISFCENTQIAK
CCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCEEEEEEHHHCCCCCCCCCCCCHHHH
ICGRDALHQGIAVVTEKLSQPELRDVLKSTSKRSLVIILDSITDPFNIGNILRSSKAFGV
HHCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCC
DALVVKERHSPSETSVIAKTASGALESIPVCTIANLTNAVKILQENGYFCYALDGGARSK
EEEEEECCCCCCHHHEEEEHHCCCHHHCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
LTEIRFPNKVAFVLGSEGKGISHLLKTRCDQTLSIPMYSDKNFDSINVSNAAAIAMFRFY
EEEECCCCEEEEEECCCCCCHHHHHHHHCCCEEECEEECCCCCCEECCCCHHHHHHEEEE
SAE
CCC
>Mature Secondary Structure
MQQEKVVIFGKHAAVAAIKNPERNILSIFIAREKKAVYLPLLERSRLPNISFCENTQIAK
CCCCEEEEEECCEEEEEECCCCCCEEEEEEECCCCEEEEEEHHHCCCCCCCCCCCCHHHH
ICGRDALHQGIAVVTEKLSQPELRDVLKSTSKRSLVIILDSITDPFNIGNILRSSKAFGV
HHCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCC
DALVVKERHSPSETSVIAKTASGALESIPVCTIANLTNAVKILQENGYFCYALDGGARSK
EEEEEECCCCCCHHHEEEEHHCCCHHHCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
LTEIRFPNKVAFVLGSEGKGISHLLKTRCDQTLSIPMYSDKNFDSINVSNAAAIAMFRFY
EEEECCCCEEEEEECCCCCCHHHHHHHHCCCEEECEEECCCCCCEECCCCHHHHHHEEEE
SAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]