Click here to switch to the map view.

The map label for this gene is pmbA

Identifier: 88608120

GI number: 88608120

Start: 478825

End: 480198

Strand: Direct

Name: pmbA

Synonym: NSE_0549

Alternate gene names: 88608120

Gene position: 478825-480198 (Clockwise)

Preceding gene: 88608763

Following gene: 88608818

Centisome position: 55.74

GC content: 37.99

Gene sequence:

>1374_bases
TTGCGAATTGAGAGCAAGATACTTAAGTTTTATAAAGATCTCAAAAATACCAAAATGAACGTCAAAAATTGCATAGAAGT
CATATTAAGCGAAGCAAGAAAACGAGATTGCCAAGCAGATTTATTGCTGACTCAAACTCAAAGCACGATCGCGAACGCAA
GAAACTTCGAAATAGAAAAACTAGAGGAATCTTTATTCTCGAGCGTTACGTTTAGACTAATAAAAGACAAGAAGGTAACC
TCTATAAGATGTGACCCTTCTGCAGATGTCCGACGCTTAGTTTCCCAAGCATACGATACATTAGCATATATTCCAGAAAA
TGAATACGCTTCACTACCACAAAATGTGGCAGTAATTGATGATATTTATAGTACTCACACTGACCACAACTTTCCCGATA
AATTAAAGGAGTTGGCAATCGAAACTGAGCAAATAGCCCACTCAAATGGGAAACTAAAAACCACAGAAACCATTGAATTT
GAAAAAAAAATCATAAAAAAAATAGTTGCAAATACGAATGGGTTTTCTGGAACTTTTCAAAACCAACTATTCTCTGCTGT
AGTAGGCGTAGTCGCGGAAAATGAAGGCAACCTGAATGCAGGTTATTCATACGTCACTAGCAACTCGCTTAAGGACCTCA
TCCCAGCAGAATTAGCAGCCGAAGCAGAAAAAAGAGCTATAGAAGGCCTAAAACCACGGAAAATCCCGACGTGCAAACGA
GAAGTTATATTTGATGCAAGGTGCGCTTCGACTTTTCTCAACAACTTTAGCAGTTTCCTTAGTGGCAAATCAGTTACCCA
ATCAGCAACCTTTTTAAAAAATTATATGGATAAAGAGATACTTCCAAGCAGTATAAACATATTGAACAATCCAGAAGGAG
CAAACTGTAAAATCGAAACACCAGCCTTTGATGATGAGGGAAACTACACGAGCAAATGCTTTCCGCTAATAGAAAAAGGG
ATATTAAAAAACTGGATACTTGATCAGTACAACGCCAACAAACTTGGCTTACAATCAAATGGTTTTGCCAAACGCACCCT
TAGCGGAAGCATCCTTCCTTTAGTGACAAATATTTATCTAGAAAGCAGCGATACATATTCTGAAAAGGAACTGATAAGTA
GAATCAAGGAAGGGTTGTACGTAACAAGCTTATTTAGCTGCGGTATTAACCCAATAACCGGAGACTATAGCCAAGGAGTT
CAAGGGTTATGGATTGAAAATGGGCAACTTTCGTTTCCCGTGAGCGAGATTACGATTGCTGGACAACTAAAAGATGTTTT
CAAAGAAATTATCGCAGCTAATAACATAAAGTTTTTTGGTAGAGCGAACTCTCCTTCTCTATATCTCGGATTTATGTCTA
TTGGCGGAACATGA

Upstream 100 bases:

>100_bases
ATGATGAATGAGATCGAATTATTACATTTATGATGTTTGCCGGATAACGTTACTAGTGTAATAAAATCCATAAAACATCA
GCAAATGTAATAACAACGCA

Downstream 100 bases:

>100_bases
AATAAAAATATTTCCTACTTTAAAATAGGCTTTTAATAAAACCCTACTTTAAAGTGGGGTTTCATCTTAACCAAAACTCA
TGCTAGTCCATGCAGTGACT

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRIESKILKFYKDLKNTKMNVKNCIEVILSEARKRDCQADLLLTQTQSTIANARNFEIEKLEESLFSSVTFRLIKDKKVT
SIRCDPSADVRRLVSQAYDTLAYIPENEYASLPQNVAVIDDIYSTHTDHNFPDKLKELAIETEQIAHSNGKLKTTETIEF
EKKIIKKIVANTNGFSGTFQNQLFSAVVGVVAENEGNLNAGYSYVTSNSLKDLIPAELAAEAEKRAIEGLKPRKIPTCKR
EVIFDARCASTFLNNFSSFLSGKSVTQSATFLKNYMDKEILPSSINILNNPEGANCKIETPAFDDEGNYTSKCFPLIEKG
ILKNWILDQYNANKLGLQSNGFAKRTLSGSILPLVTNIYLESSDTYSEKELISRIKEGLYVTSLFSCGINPITGDYSQGV
QGLWIENGQLSFPVSEITIAGQLKDVFKEIIAANNIKFFGRANSPSLYLGFMSIGGT

Sequences:

>Translated_457_residues
MRIESKILKFYKDLKNTKMNVKNCIEVILSEARKRDCQADLLLTQTQSTIANARNFEIEKLEESLFSSVTFRLIKDKKVT
SIRCDPSADVRRLVSQAYDTLAYIPENEYASLPQNVAVIDDIYSTHTDHNFPDKLKELAIETEQIAHSNGKLKTTETIEF
EKKIIKKIVANTNGFSGTFQNQLFSAVVGVVAENEGNLNAGYSYVTSNSLKDLIPAELAAEAEKRAIEGLKPRKIPTCKR
EVIFDARCASTFLNNFSSFLSGKSVTQSATFLKNYMDKEILPSSINILNNPEGANCKIETPAFDDEGNYTSKCFPLIEKG
ILKNWILDQYNANKLGLQSNGFAKRTLSGSILPLVTNIYLESSDTYSEKELISRIKEGLYVTSLFSCGINPITGDYSQGV
QGLWIENGQLSFPVSEITIAGQLKDVFKEIIAANNIKFFGRANSPSLYLGFMSIGGT
>Mature_457_residues
MRIESKILKFYKDLKNTKMNVKNCIEVILSEARKRDCQADLLLTQTQSTIANARNFEIEKLEESLFSSVTFRLIKDKKVT
SIRCDPSADVRRLVSQAYDTLAYIPENEYASLPQNVAVIDDIYSTHTDHNFPDKLKELAIETEQIAHSNGKLKTTETIEF
EKKIIKKIVANTNGFSGTFQNQLFSAVVGVVAENEGNLNAGYSYVTSNSLKDLIPAELAAEAEKRAIEGLKPRKIPTCKR
EVIFDARCASTFLNNFSSFLSGKSVTQSATFLKNYMDKEILPSSINILNNPEGANCKIETPAFDDEGNYTSKCFPLIEKG
ILKNWILDQYNANKLGLQSNGFAKRTLSGSILPLVTNIYLESSDTYSEKELISRIKEGLYVTSLFSCGINPITGDYSQGV
QGLWIENGQLSFPVSEITIAGQLKDVFKEIIAANNIKFFGRANSPSLYLGFMSIGGT

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=458, Percent_Identity=28.82096069869, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 50867; Mature: 50867

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIESKILKFYKDLKNTKMNVKNCIEVILSEARKRDCQADLLLTQTQSTIANARNFEIEK
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHCCCCHHHH
LEESLFSSVTFRLIKDKKVTSIRCDPSADVRRLVSQAYDTLAYIPENEYASLPQNVAVID
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCHHHCCCCCHHHHH
DIYSTHTDHNFPDKLKELAIETEQIAHSNGKLKTTETIEFEKKIIKKIVANTNGFSGTFQ
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCHHH
NQLFSAVVGVVAENEGNLNAGYSYVTSNSLKDLIPAELAAEAEKRAIEGLKPRKIPTCKR
HHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
EVIFDARCASTFLNNFSSFLSGKSVTQSATFLKNYMDKEILPSSINILNNPEGANCKIET
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEC
PAFDDEGNYTSKCFPLIEKGILKNWILDQYNANKLGLQSNGFAKRTLSGSILPLVTNIYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHEEEE
ESSDTYSEKELISRIKEGLYVTSLFSCGINPITGDYSQGVQGLWIENGQLSFPVSEITIA
ECCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHCCCCEEEEECCEEECCHHHHEEH
GQLKDVFKEIIAANNIKFFGRANSPSLYLGFMSIGGT
HHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCC
>Mature Secondary Structure
MRIESKILKFYKDLKNTKMNVKNCIEVILSEARKRDCQADLLLTQTQSTIANARNFEIEK
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHCCCCHHHH
LEESLFSSVTFRLIKDKKVTSIRCDPSADVRRLVSQAYDTLAYIPENEYASLPQNVAVID
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCHHHCCCCCHHHHH
DIYSTHTDHNFPDKLKELAIETEQIAHSNGKLKTTETIEFEKKIIKKIVANTNGFSGTFQ
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCHHH
NQLFSAVVGVVAENEGNLNAGYSYVTSNSLKDLIPAELAAEAEKRAIEGLKPRKIPTCKR
HHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
EVIFDARCASTFLNNFSSFLSGKSVTQSATFLKNYMDKEILPSSINILNNPEGANCKIET
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEC
PAFDDEGNYTSKCFPLIEKGILKNWILDQYNANKLGLQSNGFAKRTLSGSILPLVTNIYL
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCHHHHHHEEEE
ESSDTYSEKELISRIKEGLYVTSLFSCGINPITGDYSQGVQGLWIENGQLSFPVSEITIA
ECCCCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHCCCCEEEEECCEEECCHHHHEEH
GQLKDVFKEIIAANNIKFFGRANSPSLYLGFMSIGGT
HHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]