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The map label for this gene is bioF [H]

Identifier: 88607919

GI number: 88607919

Start: 1037655

End: 1038476

Strand: Direct

Name: bioF [H]

Synonym: APH_0979

Alternate gene names: 88607919

Gene position: 1037655-1038476 (Clockwise)

Preceding gene: 88607862

Following gene: 88607812

Centisome position: 70.53

GC content: 42.46

Gene sequence:

>822_bases
TTGGTATTTAGCAGCGGATATACAACAAATATAGGAAGCATCTCAGCATTGGTAGGTCGCCATGATATGATCTTGGCAGA
TAAGTTCATACACGCTTCTTCTCTTGATGGAGCAAGATTATCTGGAGCTACAATATACAGGTTTACGCACAATAACGTAG
ATCACTGCATGCAACTAATTAGTAAGTATAGGGAACTACACGATAACTGCCTAATTCTGCTAGAAAACATATACGGGGTA
GATGGTGACTTAGCTCCCGTAGACGAGTTTATCCAACTAGCAAAAGAGTGGAATGCTTGGGTCATTGTAGATACTGCTCA
TGGCTTTGGTATGCTCAGTTCCTTACAAGCAGACATATATGTGGGTACACTTTCCAAGGCAATGGGGGCACTGGGTGGAT
TTGTGTGCTCATCAAAAATTACGATTGAGTACTTATTAAATAAGTCACGCAGTTTTATTTATACAACTGCTTTACCACCG
GCAATTATAGCAGCTGCAAGTGCGTCTATAGACCTTTTCAAATCTTACGCAGAAGTTCCGATGCGTCTTGCAAAAGAATT
TTGCAAAATTATGGAACTACCTGAACCGCAAAGTCACATAGTGCCTTTGATTATGGAAGATGTCAGAAGCGTGATACTTG
CTGAGCAAGCCCTAGCTGAACGGGGCATTTTAGTATCAGCAATTCGTCCTCCGACAGCCCCAACTCCGAGACTGAGGTTA
ACATTTACTGCTGCACACAAGCTAATAGACGTACATAGACTGTGTAATGCATTGAAAGCGTGCGTTGTACTTACAGAGGC
AGGAAACTGGCGGGTCCTATAG

Upstream 100 bases:

>100_bases
ACTTCACATTTGGAAACTGGCAATGGTATGAAAAACCTTACTAATAAAGTACGCAGAAATAGAAGCAAAACTTGCATCAC
TATACGGAACAGAAGCTGCG

Downstream 100 bases:

>100_bases
TAGCTTCACTCTTACACATCTCTACCCCCATTCGGGAATAGAAACAGGGCACAAGAGAAGTTACCCTGCTCCACCTCAAA
AAGCGAACCTAACGCCAAAC

Product: 8-amino-7-oxononanoate synthase, truncation

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVFSSGYTTNIGSISALVGRHDMILADKFIHASSLDGARLSGATIYRFTHNNVDHCMQLISKYRELHDNCLILLENIYGV
DGDLAPVDEFIQLAKEWNAWVIVDTAHGFGMLSSLQADIYVGTLSKAMGALGGFVCSSKITIEYLLNKSRSFIYTTALPP
AIIAAASASIDLFKSYAEVPMRLAKEFCKIMELPEPQSHIVPLIMEDVRSVILAEQALAERGILVSAIRPPTAPTPRLRL
TFTAAHKLIDVHRLCNALKACVVLTEAGNWRVL

Sequences:

>Translated_273_residues
MVFSSGYTTNIGSISALVGRHDMILADKFIHASSLDGARLSGATIYRFTHNNVDHCMQLISKYRELHDNCLILLENIYGV
DGDLAPVDEFIQLAKEWNAWVIVDTAHGFGMLSSLQADIYVGTLSKAMGALGGFVCSSKITIEYLLNKSRSFIYTTALPP
AIIAAASASIDLFKSYAEVPMRLAKEFCKIMELPEPQSHIVPLIMEDVRSVILAEQALAERGILVSAIRPPTAPTPRLRL
TFTAAHKLIDVHRLCNALKACVVLTEAGNWRVL
>Mature_273_residues
MVFSSGYTTNIGSISALVGRHDMILADKFIHASSLDGARLSGATIYRFTHNNVDHCMQLISKYRELHDNCLILLENIYGV
DGDLAPVDEFIQLAKEWNAWVIVDTAHGFGMLSSLQADIYVGTLSKAMGALGGFVCSSKITIEYLLNKSRSFIYTTALPP
AIIAAASASIDLFKSYAEVPMRLAKEFCKIMELPEPQSHIVPLIMEDVRSVILAEQALAERGILVSAIRPPTAPTPRLRL
TFTAAHKLIDVHRLCNALKACVVLTEAGNWRVL

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758668, Length=285, Percent_Identity=30.5263157894737, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI284448556, Length=283, Percent_Identity=28.9752650176678, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI7657118, Length=283, Percent_Identity=28.9752650176678, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI119220554, Length=284, Percent_Identity=28.8732394366197, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI83977444, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI83977440, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI83977442, Length=249, Percent_Identity=30.1204819277108, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4502025, Length=273, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI40316939, Length=273, Percent_Identity=28.5714285714286, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786993, Length=281, Percent_Identity=35.2313167259786, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1790046, Length=282, Percent_Identity=28.3687943262411, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71994529, Length=283, Percent_Identity=26.5017667844523, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32566772, Length=284, Percent_Identity=26.4084507042254, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17560912, Length=193, Percent_Identity=29.0155440414508, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560914, Length=193, Percent_Identity=29.0155440414508, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320438, Length=297, Percent_Identity=28.956228956229, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320267, Length=284, Percent_Identity=21.830985915493, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323954, Length=194, Percent_Identity=25.2577319587629, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24662918, Length=282, Percent_Identity=28.0141843971631, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136286, Length=184, Percent_Identity=30.4347826086957, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137420, Length=264, Percent_Identity=26.8939393939394, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653280, Length=201, Percent_Identity=22.8855721393035, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653276, Length=201, Percent_Identity=22.8855721393035, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653278, Length=201, Percent_Identity=22.8855721393035, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 29961; Mature: 29961

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSSGYTTNIGSISALVGRHDMILADKFIHASSLDGARLSGATIYRFTHNNVDHCMQLI
CEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHH
SKYRELHDNCLILLENIYGVDGDLAPVDEFIQLAKEWNAWVIVDTAHGFGMLSSLQADIY
HHHHHHCCHHEEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHE
VGTLSKAMGALGGFVCSSKITIEYLLNKSRSFIYTTALPPAIIAAASASIDLFKSYAEVP
EHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MRLAKEFCKIMELPEPQSHIVPLIMEDVRSVILAEQALAERGILVSAIRPPTAPTPRLRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
TFTAAHKLIDVHRLCNALKACVVLTEAGNWRVL
EEHHHHHHHHHHHHHHHHHHHEEEECCCCEEEC
>Mature Secondary Structure
MVFSSGYTTNIGSISALVGRHDMILADKFIHASSLDGARLSGATIYRFTHNNVDHCMQLI
CEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHH
SKYRELHDNCLILLENIYGVDGDLAPVDEFIQLAKEWNAWVIVDTAHGFGMLSSLQADIY
HHHHHHCCHHEEEEHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHE
VGTLSKAMGALGGFVCSSKITIEYLLNKSRSFIYTTALPPAIIAAASASIDLFKSYAEVP
EHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MRLAKEFCKIMELPEPQSHIVPLIMEDVRSVILAEQALAERGILVSAIRPPTAPTPRLRL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEE
TFTAAHKLIDVHRLCNALKACVVLTEAGNWRVL
EEHHHHHHHHHHHHHHHHHHHEEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA