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The map label for this gene is pdhB [H]

Identifier: 88607662

GI number: 88607662

Start: 1374981

End: 1375979

Strand: Reverse

Name: pdhB [H]

Synonym: APH_1308

Alternate gene names: 88607662

Gene position: 1375979-1374981 (Counterclockwise)

Preceding gene: 88607844

Following gene: 88607765

Centisome position: 93.52

GC content: 44.64

Gene sequence:

>999_bases
ATGGAAGTTATAACTGTAAGGGAAGCATTACGCTTGGCTATGGAAGAGGAGATGGAGCGTGACCAGAGTGTTTTTCTTAT
GGGGGAAGAAGTTGGAGAATACCAAGGTGCGTACAAAATAAGTCAGGGGCTTTTAGAGCGCTTTGGGCCACAAAGGGTTG
TCGATACTCCTATTAGTGAACACGGTTTTACTGGATTGGCCGTAGGGGCGGCTTTTTGCGGATTAAAGCCTATAGTAGAG
TTTATGAGCTTTAATTTCTCTATGCAGGCTATGGACCAGATAGTTAATTCCGCTGCGAAAACAAATTACATGTCAGGGGG
GCAGCTAGGTTGTCCTATAGTCTTCCGTGGGCCTAATGGTGCTGCAGCAGGTGTTGCAGCTCAACACTCCCAGTGTTTTG
CATCGTGGTATTCACACGTGCCTGGTATAAAGGTTGTTGCTCCATATTTTGCTGCGGATTGTAAGGGGTTATTGAAATCT
GCAATACGCGATCCAAATCCTGTAATATTTCTAGAAAATGAGATTGCGTACGGACACTCGCACGAGGTGACAGAAGAGCA
GTTGAGCAAAGATAGTTTGGTTGAGCTTGGTAAAGCGGCAATTGTGAGAGAAGGTAAAGATGTAACGATTATAACATTCT
CTTTACAGTTGAAGTATGCTCTTGAGGCTGCTGAGATTCTGTTGAAGGACAATATCAGTGCGGAGGTTATAGATCTTAGG
ACATTGCGTCCTCTGGATACTGAGGCTATTTTAAAGTCTGTCAAAAAGACGAATCGTGTCGTTACTGTTGAAGAGGGATG
GCCGTTTTGTGGTGTGGGAGCAGAGATTACAGCGTTGATAGATGAATGTGCCTTTGATGATCTTGATGCACCTGTCACGA
GGGTAACTGCCAAAGATGTTCCGTTACCTTATGCAGCCAATTTGGAAAGTCTCGCCCTCCCTGGCGTGGAAGATATAGTC
TCAGCAGTGCATAAGGTGTGTAATTATTCTATAGCTTAG

Upstream 100 bases:

>100_bases
TTATTACTCCTTTACGTGAGAGTGTGCGAGAGCAAATGTATTTGCATTCTTGCTCCTTTTAAAATAAGATGAGCGCTGCA
GTTTTTACCTCTGGGGATAT

Downstream 100 bases:

>100_bases
TTGTGCTGGCACAATAGAGGCTTGCTCTGCGGTGGGGGTGCATTATCTTTTTATTTGGTCTTTTGTTTCGTTAATTGGGT
CGGGGATACGCGAGCTCCAG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEVITVREALRLAMEEEMERDQSVFLMGEEVGEYQGAYKISQGLLERFGPQRVVDTPISEHGFTGLAVGAAFCGLKPIVE
FMSFNFSMQAMDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAAGVAAQHSQCFASWYSHVPGIKVVAPYFAADCKGLLKS
AIRDPNPVIFLENEIAYGHSHEVTEEQLSKDSLVELGKAAIVREGKDVTIITFSLQLKYALEAAEILLKDNISAEVIDLR
TLRPLDTEAILKSVKKTNRVVTVEEGWPFCGVGAEITALIDECAFDDLDAPVTRVTAKDVPLPYAANLESLALPGVEDIV
SAVHKVCNYSIA

Sequences:

>Translated_332_residues
MEVITVREALRLAMEEEMERDQSVFLMGEEVGEYQGAYKISQGLLERFGPQRVVDTPISEHGFTGLAVGAAFCGLKPIVE
FMSFNFSMQAMDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAAGVAAQHSQCFASWYSHVPGIKVVAPYFAADCKGLLKS
AIRDPNPVIFLENEIAYGHSHEVTEEQLSKDSLVELGKAAIVREGKDVTIITFSLQLKYALEAAEILLKDNISAEVIDLR
TLRPLDTEAILKSVKKTNRVVTVEEGWPFCGVGAEITALIDECAFDDLDAPVTRVTAKDVPLPYAANLESLALPGVEDIV
SAVHKVCNYSIA
>Mature_332_residues
MEVITVREALRLAMEEEMERDQSVFLMGEEVGEYQGAYKISQGLLERFGPQRVVDTPISEHGFTGLAVGAAFCGLKPIVE
FMSFNFSMQAMDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAAGVAAQHSQCFASWYSHVPGIKVVAPYFAADCKGLLKS
AIRDPNPVIFLENEIAYGHSHEVTEEQLSKDSLVELGKAAIVREGKDVTIITFSLQLKYALEAAEILLKDNISAEVIDLR
TLRPLDTEAILKSVKKTNRVVTVEEGWPFCGVGAEITALIDECAFDDLDAPVTRVTAKDVPLPYAANLESLALPGVEDIV
SAVHKVCNYSIA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=59.2024539877301, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=55.2147239263804, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI4557353, Length=324, Percent_Identity=34.8765432098765, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI34101272, Length=324, Percent_Identity=34.8765432098765, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI205277463, Length=254, Percent_Identity=28.740157480315, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI4507521, Length=254, Percent_Identity=28.740157480315, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786622, Length=249, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=62.2699386503067, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17506935, Length=327, Percent_Identity=38.5321100917431, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6319698, Length=329, Percent_Identity=62.9179331306991, Blast_Score=424, Evalue=1e-120,
Organism=Drosophila melanogaster, GI21358145, Length=324, Percent_Identity=58.3333333333333, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24650940, Length=324, Percent_Identity=58.3333333333333, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI160714832, Length=324, Percent_Identity=38.2716049382716, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI160714828, Length=324, Percent_Identity=38.2716049382716, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=57.7777777777778, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=57.7777777777778, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI45551847, Length=258, Percent_Identity=28.2945736434109, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550715, Length=258, Percent_Identity=28.2945736434109, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24645119, Length=258, Percent_Identity=28.2945736434109, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36019; Mature: 36019

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVITVREALRLAMEEEMERDQSVFLMGEEVGEYQGAYKISQGLLERFGPQRVVDTPISE
CCEEEHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
HGFTGLAVGAAFCGLKPIVEFMSFNFSMQAMDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAAGVAAQHSQCFASWYSHVPGIKVVAPYFAADCKGLLKSAIRDPNPVIFLENEIAYGHS
CCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVTEEQLSKDSLVELGKAAIVREGKDVTIITFSLQLKYALEAAEILLKDNISAEVIDLR
CCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEEEEE
TLRPLDTEAILKSVKKTNRVVTVEEGWPFCGVGAEITALIDECAFDDLDAPVTRVTAKDV
CCCCCCHHHHHHHHHHCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCHHHEECCCC
PLPYAANLESLALPGVEDIVSAVHKVCNYSIA
CCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEVITVREALRLAMEEEMERDQSVFLMGEEVGEYQGAYKISQGLLERFGPQRVVDTPISE
CCEEEHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
HGFTGLAVGAAFCGLKPIVEFMSFNFSMQAMDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAAGVAAQHSQCFASWYSHVPGIKVVAPYFAADCKGLLKSAIRDPNPVIFLENEIAYGHS
CCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVTEEQLSKDSLVELGKAAIVREGKDVTIITFSLQLKYALEAAEILLKDNISAEVIDLR
CCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEEEEE
TLRPLDTEAILKSVKKTNRVVTVEEGWPFCGVGAEITALIDECAFDDLDAPVTRVTAKDV
CCCCCCHHHHHHHHHHCCCEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCHHHEECCCC
PLPYAANLESLALPGVEDIVSAVHKVCNYSIA
CCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]