Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 88607296

GI number: 88607296

Start: 1315208

End: 1316446

Strand: Reverse

Name: tyrS

Synonym: APH_1244

Alternate gene names: 88607296

Gene position: 1316446-1315208 (Counterclockwise)

Preceding gene: 88607202

Following gene: 88607512

Centisome position: 89.48

GC content: 43.42

Gene sequence:

>1239_bases
ATGGAATTTAATTCAGAATTTCTGGCTACTCTTTGTGCGCGGGGTTACCTGCACCAACTCACTGATGCTACAGCTTTAGA
CGGTATAATGAGCTCCGGGGCAGTAACCGCGTATATTGGCTTTGATTGTACGGCTAATAGCCTACATATTGGAAGCTTGA
TGCAGATTATGCTTCTCAGGTATCTTCAAAGATTCGGACACAAGCCCATCGTGCTTTTGGGAGGTGCCACTACCAAGGTA
GGTGATCCTTCCGGGAAGGAGAAAACAAGAGCCATGCTCTCCGAAGATAACATATCTGCAAATAAAGAGGGCATACTAAG
AGTAATTAAAAAATTTCTGTCAGAGGACGTTGTAGTTGTAGATAACGCCGAATGGCTTGGTAACTATGGTTATCTGGATT
TTTTAAGGGAAATTGGTAGTAAATTCTCAGTAAACGTGATGCTAGGGCTTGAGAGCGTAAAGAGTCGCCTCAGCAGGGAT
CAACAATTAAGTTTTTTGGAATTTAGCTACGTGCTGCTTCAGTCATACGATTTTGTGGAACTACACAAAAGATATGGTTG
TATTTTGCAGATAGGTGGGTCCGATCAATGGGGTAATATCGTCAGTGGTATTGATCTGGCAAGAAAGATGGGGTGTCCAC
AGCTCTACGGAGTGACCACTCCACTGCTGCTTAATAGTTCTGGAGCTAAAATGGGGAAGACAGCCGATGGCGCCATCTGG
TTGGATGAAACTTTGTATAGTCCCTATAATTATTGGCAGTATTTTCGTAATGTTCCCGATCAAGACGTAGGCCGATTGCT
TAGATTGTTTACGGAACTGCCTCTTAGTGAAATAGAGAGGTTGGAAGCCTTGAAAGGAGAGGAACTGAATGAGGCAAAAA
AGATATTGGCCACAGCGGTAACTAGTATATGCCACGGTGCTGAGGTGGCTCTGCAAGTTGAGAATCAAGCACTAAAAGTT
TTCGAACACAACGATCATGATGAACTGCGCAGTATTACCTTTAGTAGAAAGACTCTAGGCCAGGGAATCCCTATTTCCAA
GTTGCTCCATATGTGGGGGCTTGAGGAGAGTATTAGTGCTGGGCGAAGGCTCATCAGGGGAGGTGGATGTAAAATTAATG
GTGGTGTTGTCTTAGATGAAGAGAAGCTCTTGAATTGCGGAGATTTTGACGATAACGGTGGATATGTTGCTGTATTTTGT
GGTAAAAAACGTCGTCTTAAGGTAGTATTAGAAGACTAA

Upstream 100 bases:

>100_bases
TCAGTGGTAGAGCAGGTGGCTTTTAATCACCGTGTCGCGCGTTCGATTCGCGCTGGGACCACTCTACGTTATTGTTGTGC
TTGTGACGGCTTCTTAACAG

Downstream 100 bases:

>100_bases
TAGCAGCATCGCGTCAGTAAGGCTCTTTTTCACGCATTTAGATATGCATTTATGACCTGCAATGTGGAGTATAGCGTAAT
TCAGCTGTGGTTTCGCAGGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MEFNSEFLATLCARGYLHQLTDATALDGIMSSGAVTAYIGFDCTANSLHIGSLMQIMLLRYLQRFGHKPIVLLGGATTKV
GDPSGKEKTRAMLSEDNISANKEGILRVIKKFLSEDVVVVDNAEWLGNYGYLDFLREIGSKFSVNVMLGLESVKSRLSRD
QQLSFLEFSYVLLQSYDFVELHKRYGCILQIGGSDQWGNIVSGIDLARKMGCPQLYGVTTPLLLNSSGAKMGKTADGAIW
LDETLYSPYNYWQYFRNVPDQDVGRLLRLFTELPLSEIERLEALKGEELNEAKKILATAVTSICHGAEVALQVENQALKV
FEHNDHDELRSITFSRKTLGQGIPISKLLHMWGLEESISAGRRLIRGGGCKINGGVVLDEEKLLNCGDFDDNGGYVAVFC
GKKRRLKVVLED

Sequences:

>Translated_412_residues
MEFNSEFLATLCARGYLHQLTDATALDGIMSSGAVTAYIGFDCTANSLHIGSLMQIMLLRYLQRFGHKPIVLLGGATTKV
GDPSGKEKTRAMLSEDNISANKEGILRVIKKFLSEDVVVVDNAEWLGNYGYLDFLREIGSKFSVNVMLGLESVKSRLSRD
QQLSFLEFSYVLLQSYDFVELHKRYGCILQIGGSDQWGNIVSGIDLARKMGCPQLYGVTTPLLLNSSGAKMGKTADGAIW
LDETLYSPYNYWQYFRNVPDQDVGRLLRLFTELPLSEIERLEALKGEELNEAKKILATAVTSICHGAEVALQVENQALKV
FEHNDHDELRSITFSRKTLGQGIPISKLLHMWGLEESISAGRRLIRGGGCKINGGVVLDEEKLLNCGDFDDNGGYVAVFC
GKKRRLKVVLED
>Mature_412_residues
MEFNSEFLATLCARGYLHQLTDATALDGIMSSGAVTAYIGFDCTANSLHIGSLMQIMLLRYLQRFGHKPIVLLGGATTKV
GDPSGKEKTRAMLSEDNISANKEGILRVIKKFLSEDVVVVDNAEWLGNYGYLDFLREIGSKFSVNVMLGLESVKSRLSRD
QQLSFLEFSYVLLQSYDFVELHKRYGCILQIGGSDQWGNIVSGIDLARKMGCPQLYGVTTPLLLNSSGAKMGKTADGAIW
LDETLYSPYNYWQYFRNVPDQDVGRLLRLFTELPLSEIERLEALKGEELNEAKKILATAVTSICHGAEVALQVENQALKV
FEHNDHDELRSITFSRKTLGQGIPISKLLHMWGLEESISAGRRLIRGGGCKINGGVVLDEEKLLNCGDFDDNGGYVAVFC
GKKRRLKVVLED

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=405, Percent_Identity=35.0617283950617, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1787925, Length=397, Percent_Identity=39.5465994962217, Blast_Score=274, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI25145802, Length=284, Percent_Identity=39.4366197183099, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI25145805, Length=202, Percent_Identity=38.6138613861386, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325160, Length=331, Percent_Identity=35.0453172205438, Blast_Score=196, Evalue=8e-51,
Organism=Drosophila melanogaster, GI20130383, Length=436, Percent_Identity=33.0275229357798, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_ANAPZ (Q2GIN1)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505770.1
- ProteinModelPortal:   Q2GIN1
- SMR:   Q2GIN1
- STRING:   Q2GIN1
- GeneID:   3930514
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_1244
- NMPDR:   fig|212042.5.peg.1192
- TIGR:   APH_1244
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q2GIN1
- ProtClustDB:   PRK05912
- BioCyc:   APHA212042:APH_1244-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45726; Mature: 45726

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 38-38 BINDING 170-170 BINDING 174-174 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNSEFLATLCARGYLHQLTDATALDGIMSSGAVTAYIGFDCTANSLHIGSLMQIMLLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
YLQRFGHKPIVLLGGATTKVGDPSGKEKTRAMLSEDNISANKEGILRVIKKFLSEDVVVV
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEE
DNAEWLGNYGYLDFLREIGSKFSVNVMLGLESVKSRLSRDQQLSFLEFSYVLLQSYDFVE
ECHHHHCCCCHHHHHHHHCCCEEEEEEECHHHHHHHHCHHHCCHHHHHHHHHHHCCCHHH
LHKRYGCILQIGGSDQWGNIVSGIDLARKMGCPQLYGVTTPLLLNSSGAKMGKTADGAIW
HHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCCEE
LDETLYSPYNYWQYFRNVPDQDVGRLLRLFTELPLSEIERLEALKGEELNEAKKILATAV
EHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
TSICHGAEVALQVENQALKVFEHNDHDELRSITFSRKTLGQGIPISKLLHMWGLEESISA
HHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
GRRLIRGGGCKINGGVVLDEEKLLNCGDFDDNGGYVAVFCGKKRRLKVVLED
HHHHHCCCCEEECCCEEECHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MEFNSEFLATLCARGYLHQLTDATALDGIMSSGAVTAYIGFDCTANSLHIGSLMQIMLLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHH
YLQRFGHKPIVLLGGATTKVGDPSGKEKTRAMLSEDNISANKEGILRVIKKFLSEDVVVV
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEE
DNAEWLGNYGYLDFLREIGSKFSVNVMLGLESVKSRLSRDQQLSFLEFSYVLLQSYDFVE
ECHHHHCCCCHHHHHHHHCCCEEEEEEECHHHHHHHHCHHHCCHHHHHHHHHHHCCCHHH
LHKRYGCILQIGGSDQWGNIVSGIDLARKMGCPQLYGVTTPLLLNSSGAKMGKTADGAIW
HHHHCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCCCCCCEE
LDETLYSPYNYWQYFRNVPDQDVGRLLRLFTELPLSEIERLEALKGEELNEAKKILATAV
EHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
TSICHGAEVALQVENQALKVFEHNDHDELRSITFSRKTLGQGIPISKLLHMWGLEESISA
HHHHCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
GRRLIRGGGCKINGGVVLDEEKLLNCGDFDDNGGYVAVFCGKKRRLKVVLED
HHHHHCCCCEEECCCEEECHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA