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The map label for this gene is gatA

Identifier: 87200845

GI number: 87200845

Start: 3059873

End: 3061357

Strand: Reverse

Name: gatA

Synonym: Saro_2832

Alternate gene names: 87200845

Gene position: 3061357-3059873 (Counterclockwise)

Preceding gene: 87200846

Following gene: 87200844

Centisome position: 85.95

GC content: 68.69

Gene sequence:

>1485_bases
ATGACCAATCTTACTGATCTCGGCATTGCCGCCATCCGCGATGGCGTGGCGCGGGGCGATTTCACCGCGACCGAAGTCGC
CACCGCGTTCAACGCGGCGGTCGAGGCGGCGCAGCCCGCGCTCAACGCCTTTATCGTCACCACACCGGAAGAGGCGTTGG
AAGCCGCCGGGAAGGTCGATGCCGACCGCACCGCCGGAAGGCCGCTGGGCAAGATGGCGGGCGTGCCGATCGGCATGAAG
GACCTCTTCGCGACGCGCGGCACCCAGACCACCGCCGCCTCGAAGATCCTCGAAGGCTTCATGCCCGAGTACGAATCGAC
CGTTTCGCAGAAGCTGTGGGATGCCGGCGCGGGCATGCTCGGCAAGCTCAACCTCGACCAGTTCGCGATGGGCTCCTCGA
ACGAGACCAGCGCTTTCGGCAACGTGATCTCGCCGTGGCGCCGTCCGGGCGACACCGCGCCGCTCGCGCCCGGCGGATCT
TCGGGCGGGTCGTCCTCGGCGGTCGCCGCACGCATCGCGCCTGCCGCCACCGGCACCGACACCGGCGGTTCGATCCGCCA
GCCCGCCGCGTTCACCGGCATCTCTGGCATCAAGCCGACTTACGGGCGTTGCTCGCGCTGGGGCATCGTCGCCTTCGCGT
CCAGCCTCGACCAGGCCGGCCCGATGGCCCGCGACGTCGCCGATTGCGCGATCATGCTCGAAGCCATGGCCGGTTTCGAT
CCCAAGGATTCCACCAGCCTCGACATGCCCGTCCCGGAATGGACCGCGAACCTCGATCCCGACATGCGCGGCAAGAAGGT
CGGCATCCCTCGCGAATACCGGCTCGACGGCATGGACCCCGACGTTGCCAGGTCGTGGGAAGACGGCATCGCATGGCTCA
AGGATGCGGGCGCCGAGATCGTCGAGATCAGCCTGCCCCACACGAAGTACGCGCTGCCCACCTACTACATCATCGCCCCG
GCAGAGGCGTCCTCGAACCTCGCCCGCTACGATGGCGTGCGTTACGGCCTGCGCGACCTTCCGGAAGGCGCGGGCCTCCA
GGACATGTACGCCGCCACCCGCGCCGCCGGGTTCGGCCCCGAGGTGAAGCGCCGCATCCTGATCGGCACCTACGTGCTCT
CGGCCGGTTTCTACGACGCCTACTACACCCAGGCGCAGAAGGTCCGCACGCTGATCAGCCACGACTTCACCAATGCCTTC
CGCGAAGTCGACGTGATCCTCGCGCCGACCGCGCCGTCCTCGGCCTTCGCGCTGGGCGAGAAGAGCGCCGATCCGCTGGA
GATGTACCTGAACGACGTGTTCTCGGTCCCGGCCAGCCTGGCCGGCCTCCCGGCGATGTCGGTCCCGGCTGGGCTCGACC
GCAACGGCCTCCCCCTCGGCCTCCAGGTCATCGGCCGCGCCTTCGACGAACAGGGCGTCCTCAACGCGGGCCTCGCCCTC
GAACAGCGCGCCCGGTTCTCCGCCCGTCCGGCAAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
ACGCCGACCCGCTGACCGGCGGCGACATGCGCGATGCCGTCCTGGCCAACGCGCCCGCCCCCGAACACGGCTTCTTCGGC
GTGCCCAAGGTGATCGAATA

Downstream 100 bases:

>100_bases
GATGAGCGAATATCGTATCCAGGGCGCAACCGGCGAATGGGAGGTCGTGATCGGCCTCGAAGTCCATGCGCAGGTCACTT
CCAACGCCAAGCTGTTCTCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTNLTDLGIAAIRDGVARGDFTATEVATAFNAAVEAAQPALNAFIVTTPEEALEAAGKVDADRTAGRPLGKMAGVPIGMK
DLFATRGTQTTAASKILEGFMPEYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSAFGNVISPWRRPGDTAPLAPGGS
SGGSSSAVAARIAPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVADCAIMLEAMAGFD
PKDSTSLDMPVPEWTANLDPDMRGKKVGIPREYRLDGMDPDVARSWEDGIAWLKDAGAEIVEISLPHTKYALPTYYIIAP
AEASSNLARYDGVRYGLRDLPEGAGLQDMYAATRAAGFGPEVKRRILIGTYVLSAGFYDAYYTQAQKVRTLISHDFTNAF
REVDVILAPTAPSSAFALGEKSADPLEMYLNDVFSVPASLAGLPAMSVPAGLDRNGLPLGLQVIGRAFDEQGVLNAGLAL
EQRARFSARPAKWW

Sequences:

>Translated_494_residues
MTNLTDLGIAAIRDGVARGDFTATEVATAFNAAVEAAQPALNAFIVTTPEEALEAAGKVDADRTAGRPLGKMAGVPIGMK
DLFATRGTQTTAASKILEGFMPEYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSAFGNVISPWRRPGDTAPLAPGGS
SGGSSSAVAARIAPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVADCAIMLEAMAGFD
PKDSTSLDMPVPEWTANLDPDMRGKKVGIPREYRLDGMDPDVARSWEDGIAWLKDAGAEIVEISLPHTKYALPTYYIIAP
AEASSNLARYDGVRYGLRDLPEGAGLQDMYAATRAAGFGPEVKRRILIGTYVLSAGFYDAYYTQAQKVRTLISHDFTNAF
REVDVILAPTAPSSAFALGEKSADPLEMYLNDVFSVPASLAGLPAMSVPAGLDRNGLPLGLQVIGRAFDEQGVLNAGLAL
EQRARFSARPAKWW
>Mature_493_residues
TNLTDLGIAAIRDGVARGDFTATEVATAFNAAVEAAQPALNAFIVTTPEEALEAAGKVDADRTAGRPLGKMAGVPIGMKD
LFATRGTQTTAASKILEGFMPEYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSAFGNVISPWRRPGDTAPLAPGGSS
GGSSSAVAARIAPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVADCAIMLEAMAGFDP
KDSTSLDMPVPEWTANLDPDMRGKKVGIPREYRLDGMDPDVARSWEDGIAWLKDAGAEIVEISLPHTKYALPTYYIIAPA
EASSNLARYDGVRYGLRDLPEGAGLQDMYAATRAAGFGPEVKRRILIGTYVLSAGFYDAYYTQAQKVRTLISHDFTNAFR
EVDVILAPTAPSSAFALGEKSADPLEMYLNDVFSVPASLAGLPAMSVPAGLDRNGLPLGLQVIGRAFDEQGVLNAGLALE
QRARFSARPAKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=39.8373983739837, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI195972892, Length=477, Percent_Identity=25.1572327044025, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI166795287, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=409, Percent_Identity=40.3422982885086, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=23.3067729083665, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=25.4658385093168, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=313, Percent_Identity=27.1565495207668, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=24.9190938511327, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=423, Percent_Identity=37.3522458628842, Blast_Score=255, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=453, Percent_Identity=28.0353200883002, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=246, Percent_Identity=27.6422764227642, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=462, Percent_Identity=38.3116883116883, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=23.991935483871, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=24.2362525458248, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=24.2362525458248, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=24.2362525458248, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=24.2362525458248, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=24.0917782026769, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356731, Length=490, Percent_Identity=23.8775510204082, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=26.2096774193548, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=26.2096774193548, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=26.2096774193548, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_NOVAD (Q2G4F5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498102.1
- ProteinModelPortal:   Q2G4F5
- SMR:   Q2G4F5
- STRING:   Q2G4F5
- GeneID:   3915471
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2832
- NMPDR:   fig|48935.1.peg.287
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q2G4F5
- ProtClustDB:   PRK00012
- BioCyc:   NARO279238:SARO_2832-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52104; Mature: 51973

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 160-160 ACT_SITE 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLTDLGIAAIRDGVARGDFTATEVATAFNAAVEAAQPALNAFIVTTPEEALEAAGKVD
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCC
ADRTAGRPLGKMAGVPIGMKDLFATRGTQTTAASKILEGFMPEYESTVSQKLWDAGAGML
CCHHCCCCCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCE
GKLNLDQFAMGSSNETSAFGNVISPWRRPGDTAPLAPGGSSGGSSSAVAARIAPAATGTD
ECCCHHHHCCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCC
TGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVADCAIMLEAMAGFD
CCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PKDSTSLDMPVPEWTANLDPDMRGKKVGIPREYRLDGMDPDVARSWEDGIAWLKDAGAEI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEE
VEISLPHTKYALPTYYIIAPAEASSNLARYDGVRYGLRDLPEGAGLQDMYAATRAAGFGP
EEEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCH
EVKRRILIGTYVLSAGFYDAYYTQAQKVRTLISHDFTNAFREVDVILAPTAPSSAFALGE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCC
KSADPLEMYLNDVFSVPASLAGLPAMSVPAGLDRNGLPLGLQVIGRAFDEQGVLNAGLAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCHHH
EQRARFSARPAKWW
HHHHHHCCCCCCCC
>Mature Secondary Structure 
TNLTDLGIAAIRDGVARGDFTATEVATAFNAAVEAAQPALNAFIVTTPEEALEAAGKVD
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCCC
ADRTAGRPLGKMAGVPIGMKDLFATRGTQTTAASKILEGFMPEYESTVSQKLWDAGAGML
CCHHCCCCCHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCE
GKLNLDQFAMGSSNETSAFGNVISPWRRPGDTAPLAPGGSSGGSSSAVAARIAPAATGTD
ECCCHHHHCCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCC
TGGSIRQPAAFTGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVADCAIMLEAMAGFD
CCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PKDSTSLDMPVPEWTANLDPDMRGKKVGIPREYRLDGMDPDVARSWEDGIAWLKDAGAEI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCEE
VEISLPHTKYALPTYYIIAPAEASSNLARYDGVRYGLRDLPEGAGLQDMYAATRAAGFGP
EEEECCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCH
EVKRRILIGTYVLSAGFYDAYYTQAQKVRTLISHDFTNAFREVDVILAPTAPSSAFALGE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHCCCC
KSADPLEMYLNDVFSVPASLAGLPAMSVPAGLDRNGLPLGLQVIGRAFDEQGVLNAGLAL
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCHHH
EQRARFSARPAKWW
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA