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The map label for this gene is gatB

Identifier: 87200844

GI number: 87200844

Start: 3058378

End: 3059871

Strand: Reverse

Name: gatB

Synonym: Saro_2831

Alternate gene names: 87200844

Gene position: 3059871-3058378 (Counterclockwise)

Preceding gene: 87200845

Following gene: 87200843

Centisome position: 85.91

GC content: 65.33

Gene sequence:

>1494_bases
ATGAGCGAATATCGTATCCAGGGCGCAACCGGCGAATGGGAGGTCGTGATCGGCCTCGAAGTCCATGCGCAGGTCACTTC
CAACGCCAAGCTGTTCTCGGGCGCGGCCACCGCGTTCGGCGCCGAGCCGAACACGCAGGTCAGCCTTGTCGACGCGGCGA
TGCCGGGGATGCTTCCGGTGCCCAACCGCGAATGCATCCGCCAGGCGGTGCGCACCGGCATGGCGATCAACGCGCAGATC
AACAAGTGGTCGCGCTTCGATCGCAAGAACTACTTCTACGCCGATCTTCCGCAGGGCTACCAGATCAGCCAGCTCTACCA
CCCCATCGTGGGCGAGGGCAGCATCGAGGTCCAGCTCGACGACAAGAACCCGGAAAGCCTCAAGACCATCGGGATCGAGC
GCATCCACGTCGAGCAGGACGCCGGCAAGCTGATGCACGACCAGCACCCGACGATGTCCTACGTCGACCTCAACCGCTCG
GGCGTGGCGCTTATGGAAATCGTCAGCCGTCCGGACATGCGCTCGCCGGCAGAGGCGGGGGCCTACCTGTCGAAGCTGCG
CACGATCCTGCGCTATGTCGGCAGTTGCGACGGCAACATGGACCAGGGCTCGATGCGCGCCGACGTCAACGTTTCGGTCC
GCCGCCCGGGGGAGCCTTTCGGCACCCGCACCGAGACCAAGAACGTCAACTCGGTCCGCTTCGTGATGGCCGTTGTCGAA
CAGGAGGCCAAGCGCCAGGTTGCCTTGATCGAGGACGGCGGCACGGTGGTGCAGGAAACGCGGCTCTACGATCCCGACCG
CAACGAGACCCGCTCGATGCGCTCGAAGGAAGATGCGCACGATTATCGCTACTTCCCCGATCCCGACCTCCTGCCGCTCG
AACTCGACGACGCCTTCCTCGAGGAATGCCGCGCAAGCTTGCCCGAGTTGCCCGATGCCAAGCGCCATCGCTACGAGACC
GCGCTCGGTCTTTCGGCCTACAACGCGGGCGTGCTGACGGCTGACGTCGAGACCGCGCGCTGGTTCGAGGCGCTGCTCGC
CGAGACCGCCGCGAAGGCGAAGAAGCCGGAAGCCGAGGTTGCCAAGCAGGCGGCCAACTGGCTGATCTCCGAACTCTTCG
GTGCGCTCAACAAGCTTGGCGCGAGCCTTGAAACCTCGCCGGTCACCCCGGCGGCGGGCGCGGAACTCCTCGCCCTGATC
GCCGACGGCACGATTTCGGGTTCCATCGCCAAGCAGGTGCTGGAAAAGATGCTCGAGACCGGCGACGGCGCCGCCGCCAT
CGTCGAGCGCGAAGGCCTCAAGCAGACCTCCGACACCGGCGCGATCGAAGCCGCCATCGACGGCATCCTCGCCGCCAACG
CCGACAAGGTCGGGCAGTACAAGGCAGGCAAGGAAGCCCTGTTCGGCTTCTTCGTTGGCCAGACGATGAAGGCCATGCAG
GGCAAGGCCAACCCCGGCGTGGTCAACGAACTGCTGCGCAAGAAGCTGGGGTAA

Upstream 100 bases:

>100_bases
GTCATCGGCCGCGCCTTCGACGAACAGGGCGTCCTCAACGCGGGCCTCGCCCTCGAACAGCGCGCCCGGTTCTCCGCCCG
TCCGGCAAAGTGGTGGTAAG

Downstream 100 bases:

>100_bases
TTGCCGAGGCGCGGGTAGCCTGCAATTGTAAAGGGGCGACTGTTTGATATAATAGTCGCCCAATATAGAGGGGGCTCTAG
TGCGTAAAATACTGGCTGCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSEYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAINAQI
NKWSRFDRKNYFYADLPQGYQISQLYHPIVGEGSIEVQLDDKNPESLKTIGIERIHVEQDAGKLMHDQHPTMSYVDLNRS
GVALMEIVSRPDMRSPAEAGAYLSKLRTILRYVGSCDGNMDQGSMRADVNVSVRRPGEPFGTRTETKNVNSVRFVMAVVE
QEAKRQVALIEDGGTVVQETRLYDPDRNETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRHRYET
ALGLSAYNAGVLTADVETARWFEALLAETAAKAKKPEAEVAKQAANWLISELFGALNKLGASLETSPVTPAAGAELLALI
ADGTISGSIAKQVLEKMLETGDGAAAIVEREGLKQTSDTGAIEAAIDGILAANADKVGQYKAGKEALFGFFVGQTMKAMQ
GKANPGVVNELLRKKLG

Sequences:

>Translated_497_residues
MSEYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAINAQI
NKWSRFDRKNYFYADLPQGYQISQLYHPIVGEGSIEVQLDDKNPESLKTIGIERIHVEQDAGKLMHDQHPTMSYVDLNRS
GVALMEIVSRPDMRSPAEAGAYLSKLRTILRYVGSCDGNMDQGSMRADVNVSVRRPGEPFGTRTETKNVNSVRFVMAVVE
QEAKRQVALIEDGGTVVQETRLYDPDRNETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRHRYET
ALGLSAYNAGVLTADVETARWFEALLAETAAKAKKPEAEVAKQAANWLISELFGALNKLGASLETSPVTPAAGAELLALI
ADGTISGSIAKQVLEKMLETGDGAAAIVEREGLKQTSDTGAIEAAIDGILAANADKVGQYKAGKEALFGFFVGQTMKAMQ
GKANPGVVNELLRKKLG
>Mature_496_residues
SEYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPVPNRECIRQAVRTGMAINAQIN
KWSRFDRKNYFYADLPQGYQISQLYHPIVGEGSIEVQLDDKNPESLKTIGIERIHVEQDAGKLMHDQHPTMSYVDLNRSG
VALMEIVSRPDMRSPAEAGAYLSKLRTILRYVGSCDGNMDQGSMRADVNVSVRRPGEPFGTRTETKNVNSVRFVMAVVEQ
EAKRQVALIEDGGTVVQETRLYDPDRNETRSMRSKEDAHDYRYFPDPDLLPLELDDAFLEECRASLPELPDAKRHRYETA
LGLSAYNAGVLTADVETARWFEALLAETAAKAKKPEAEVAKQAANWLISELFGALNKLGASLETSPVTPAAGAELLALIA
DGTISGSIAKQVLEKMLETGDGAAAIVEREGLKQTSDTGAIEAAIDGILAANADKVGQYKAGKEALFGFFVGQTMKAMQG
KANPGVVNELLRKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=502, Percent_Identity=40.0398406374502, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=39.5973154362416, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=517, Percent_Identity=31.3346228239845, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI45550808, Length=502, Percent_Identity=35.2589641434263, Blast_Score=298, Evalue=8e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_NOVAD (Q2G4F6)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498101.1
- ProteinModelPortal:   Q2G4F6
- SMR:   Q2G4F6
- STRING:   Q2G4F6
- GeneID:   3915470
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2831
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2G4F6
- ProtClustDB:   PRK05477
- BioCyc:   NARO279238:SARO_2831-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54106; Mature: 53975

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPV
CCCEEECCCCCCEEEEEEEEEEEEECCCCEEECCCHHHCCCCCCCEEEEEECCCCCCCCC
PNRECIRQAVRTGMAINAQINKWSRFDRKNYFYADLPQGYQISQLYHPIVGEGSIEVQLD
CCHHHHHHHHHCCEEEEEECHHHHHHCCCCCEEEECCCCCCHHHHHCHHCCCCEEEEEEC
DKNPESLKTIGIERIHVEQDAGKLMHDQHPTMSYVDLNRSGVALMEIVSRPDMRSPAEAG
CCCCHHHHHHCCEEEEEECHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHH
AYLSKLRTILRYVGSCDGNMDQGSMRADVNVSVRRPGEPFGTRTETKNVNSVRFVMAVVE
HHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
QEAKRQVALIEDGGTVVQETRLYDPDRNETRSMRSKEDAHDYRYFPDPDLLPLELDDAFL
HHHHCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHH
EECRASLPELPDAKRHRYETALGLSAYNAGVLTADVETARWFEALLAETAAKAKKPEAEV
HHHHHHCCCCCCHHHHHHHHHHCHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHH
AKQAANWLISELFGALNKLGASLETSPVTPAAGAELLALIADGTISGSIAKQVLEKMLET
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
GDGAAAIVEREGLKQTSDTGAIEAAIDGILAANADKVGQYKAGKEALFGFFVGQTMKAMQ
CCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHC
GKANPGVVNELLRKKLG
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SEYRIQGATGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPNTQVSLVDAAMPGMLPV
CCEEECCCCCCEEEEEEEEEEEEECCCCEEECCCHHHCCCCCCCEEEEEECCCCCCCCC
PNRECIRQAVRTGMAINAQINKWSRFDRKNYFYADLPQGYQISQLYHPIVGEGSIEVQLD
CCHHHHHHHHHCCEEEEEECHHHHHHCCCCCEEEECCCCCCHHHHHCHHCCCCEEEEEEC
DKNPESLKTIGIERIHVEQDAGKLMHDQHPTMSYVDLNRSGVALMEIVSRPDMRSPAEAG
CCCCHHHHHHCCEEEEEECHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCCCHHHHH
AYLSKLRTILRYVGSCDGNMDQGSMRADVNVSVRRPGEPFGTRTETKNVNSVRFVMAVVE
HHHHHHHHHHHHHHCCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
QEAKRQVALIEDGGTVVQETRLYDPDRNETRSMRSKEDAHDYRYFPDPDLLPLELDDAFL
HHHHCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHH
EECRASLPELPDAKRHRYETALGLSAYNAGVLTADVETARWFEALLAETAAKAKKPEAEV
HHHHHHCCCCCCHHHHHHHHHHCHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHH
AKQAANWLISELFGALNKLGASLETSPVTPAAGAELLALIADGTISGSIAKQVLEKMLET
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
GDGAAAIVEREGLKQTSDTGAIEAAIDGILAANADKVGQYKAGKEALFGFFVGQTMKAMQ
CCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHC
GKANPGVVNELLRKKLG
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA