Click here to switch to the map view.

The map label for this gene is amiA [H]

Identifier: 87200331

GI number: 87200331

Start: 2458867

End: 2459832

Strand: Reverse

Name: amiA [H]

Synonym: Saro_2317

Alternate gene names: 87200331

Gene position: 2459832-2458867 (Counterclockwise)

Preceding gene: 87200333

Following gene: 87200330

Centisome position: 69.07

GC content: 68.12

Gene sequence:

>966_bases
ATGCGGCCAGTTCCCCGGTCCATCACGCAGAGCCATTTGTGCTGGACCATCCCGAGCGGGTCGGGGCATAGGCAAAGTCA
GATGAAGAGCAGGGGCATCATTGCGGCGGCATTTGCAGCATCCGTTGCTGCGGCCGCGCTGATGTGGGCAGTCCTTACCA
GCGGCATTGCCACGATCGAGCCGCGTTATGTCGTGCGGATGGACATGCCGGCCCGCGAGGGGCGGATCGGCCTGCCGCCG
GTGGAAGGTCCGGCGGATACGAGCCGTCCGCTTGTGGTCATCGACGCGGGCCACGGCGGCCATGACCCGGGCGCAAGCGG
GCCGGCGGGGGAACGCGAAAAGGACCTGACGCTGCTTCTCGCAGGCGCGCTGCGCGATGCCCTGCTGGCCGACGGACGAG
TGCGGGTGGCGATGACCCGCGCCGAGGATCGCTTCCTGGTACTGGAGGAGCGGGGCGACATCGCACATCGGCTGGGCGCG
GACCTGTTCCTGTCGGTCCATGCAGATGCCGCGCAGAACGACCTGGCGCAGGGGGCGACGGTCTACACGCTGTCCGACGA
GGCTAGCGATTCGGTTGCCGAGGCCCTTGCCATGCGCGAAAACCGCGCCGACCAGGTCAACGGGGTGAAGCTGGCAGGCA
AGGGCGAGGCTGTATCCTCGATCCTTGTCGACCTTGCCCGGCGAGAAATGCGCGGACGCTCGATGCGGTTCGGCGAGCTT
GTCGTGCGCGAGGGCGATGGGCGCATCCGCTTTCACGAGACGCCCCAGCGCGAGGCCGCGTTCGTCGTGCTGAAGTCGCT
CGACCTGCCATCGGCGCTGATCGAGGCGGGCTACATCAGCAATACAGACGATGCGCGCGCGATGGCCGACCCGGCGTGGC
GCCAGACTTTTGCGGGCGTGGTGGCGCGGGCAATCGAGATTTTCCTGGCGGAAGGGCAGGCAGGCCCCGGCGTTGCGGCG
CCCTGA

Upstream 100 bases:

>100_bases
AAATCTGCGCTTTTGGCGGCTTCGGCGTGGACTTTGCCGGTGCTGGCTTGCCCCGGTAGATGGGTGCTAGCATCCCAAAT
GCAAACCAGCAATTAAAACA

Downstream 100 bases:

>100_bases
ACAGGATTTGCGACTGCAAGCTCTGGCTTGGCGGGGCGCGAGCGTGCTAGAGGCCCTGCACGATGGCCGAAGAGACCCCT
GTGCAATCCCGGTTCACCCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRPVPRSITQSHLCWTIPSGSGHRQSQMKSRGIIAAAFAASVAAAALMWAVLTSGIATIEPRYVVRMDMPAREGRIGLPP
VEGPADTSRPLVVIDAGHGGHDPGASGPAGEREKDLTLLLAGALRDALLADGRVRVAMTRAEDRFLVLEERGDIAHRLGA
DLFLSVHADAAQNDLAQGATVYTLSDEASDSVAEALAMRENRADQVNGVKLAGKGEAVSSILVDLARREMRGRSMRFGEL
VVREGDGRIRFHETPQREAAFVVLKSLDLPSALIEAGYISNTDDARAMADPAWRQTFAGVVARAIEIFLAEGQAGPGVAA
P

Sequences:

>Translated_321_residues
MRPVPRSITQSHLCWTIPSGSGHRQSQMKSRGIIAAAFAASVAAAALMWAVLTSGIATIEPRYVVRMDMPAREGRIGLPP
VEGPADTSRPLVVIDAGHGGHDPGASGPAGEREKDLTLLLAGALRDALLADGRVRVAMTRAEDRFLVLEERGDIAHRLGA
DLFLSVHADAAQNDLAQGATVYTLSDEASDSVAEALAMRENRADQVNGVKLAGKGEAVSSILVDLARREMRGRSMRFGEL
VVREGDGRIRFHETPQREAAFVVLKSLDLPSALIEAGYISNTDDARAMADPAWRQTFAGVVARAIEIFLAEGQAGPGVAA
P
>Mature_321_residues
MRPVPRSITQSHLCWTIPSGSGHRQSQMKSRGIIAAAFAASVAAAALMWAVLTSGIATIEPRYVVRMDMPAREGRIGLPP
VEGPADTSRPLVVIDAGHGGHDPGASGPAGEREKDLTLLLAGALRDALLADGRVRVAMTRAEDRFLVLEERGDIAHRLGA
DLFLSVHADAAQNDLAQGATVYTLSDEASDSVAEALAMRENRADQVNGVKLAGKGEAVSSILVDLARREMRGRSMRFGEL
VVREGDGRIRFHETPQREAAFVVLKSLDLPSALIEAGYISNTDDARAMADPAWRQTFAGVVARAIEIFLAEGQAGPGVAA
P

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=218, Percent_Identity=38.5321100917431, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082163, Length=245, Percent_Identity=34.6938775510204, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=33.1967213114754, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 34084; Mature: 34084

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVPRSITQSHLCWTIPSGSGHRQSQMKSRGIIAAAFAASVAAAALMWAVLTSGIATIE
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECC
PRYVVRMDMPAREGRIGLPPVEGPADTSRPLVVIDAGHGGHDPGASGPAGEREKDLTLLL
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
AGALRDALLADGRVRVAMTRAEDRFLVLEERGDIAHRLGADLFLSVHADAAQNDLAQGAT
HHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCHHHHCCCEEEEEEECCHHHHHHHCCCE
VYTLSDEASDSVAEALAMRENRADQVNGVKLAGKGEAVSSILVDLARREMRGRSMRFGEL
EEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEE
VVREGDGRIRFHETPQREAAFVVLKSLDLPSALIEAGYISNTDDARAMADPAWRQTFAGV
EEECCCCEEEECCCCCHHHEEEEEECCCCCHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
VARAIEIFLAEGQAGPGVAAP
HHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRPVPRSITQSHLCWTIPSGSGHRQSQMKSRGIIAAAFAASVAAAALMWAVLTSGIATIE
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEECC
PRYVVRMDMPAREGRIGLPPVEGPADTSRPLVVIDAGHGGHDPGASGPAGEREKDLTLLL
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
AGALRDALLADGRVRVAMTRAEDRFLVLEERGDIAHRLGADLFLSVHADAAQNDLAQGAT
HHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCHHHHCCCEEEEEEECCHHHHHHHCCCE
VYTLSDEASDSVAEALAMRENRADQVNGVKLAGKGEAVSSILVDLARREMRGRSMRFGEL
EEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEE
VVREGDGRIRFHETPQREAAFVVLKSLDLPSALIEAGYISNTDDARAMADPAWRQTFAGV
EEECCCCEEEECCCCCHHHEEEEEECCCCCHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
VARAIEIFLAEGQAGPGVAAP
HHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]