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The map label for this gene is thlA [H]

Identifier: 87200320

GI number: 87200320

Start: 2446328

End: 2447524

Strand: Direct

Name: thlA [H]

Synonym: Saro_2306

Alternate gene names: 87200320

Gene position: 2446328-2447524 (Clockwise)

Preceding gene: 87200295

Following gene: 87200322

Centisome position: 68.69

GC content: 68.84

Gene sequence:

>1197_bases
ATGGCACAGTTCTCCTCGTCCGATCCCGTCGTCATTCTGTCCTACGCACGCACGCCGATGGGCGCGATGCAGGGTGCCCT
GGCAGAGGTCGCCGCCACCGACCTCGGCGCCACCGCGGTCAAGGCCGCCGTTGAGCGCGCGGGCGTCGCGCCCGACAAGG
TCGAGCGCATCTACATGGGCTGCGTGCTTCCCGCCGGCCTCGGCCAGGCACCCGCACGTCAGGCCGCGATCAAGGCCGGC
CTGCCCAAGTCGGTTCAAGCGACAACCGTCAACAAGGTCTGCGGTTCGGGCATGCAGACGATCATCATGGGCGCCGAGGC
TCTGGCCGCGGGCAGCGCCGACCTGATCGTCGCGGGCGGCATGGAATCGATGACCAACGCCCCCTACCTGCTCAAGAAGC
ATCGCTCGGGCGCGCGCATCGGTCACGACATGGCCTATGACCACATGTTCCTCGACGGGCTCGAGGATGCCTACGACGCC
GGCCGCGCCATGGGCACGTTCGCGCAGGACACCGCCAACGCCTATCAACTCACCCGCGAACAGCAGGACGCCTACTCGAT
CGAATCGCTCCGCCGCGCGCAGGCCGCCGTCGCCGACGGCTCGTTCAAGGACGAAGTCGTCGCGGTCACCGTCAAGACCC
GCGCCGGTGAAGTGACGGTCGATACGGACGAGGCACCCGGCAAGGGCCGTCCCGACAAGATCCCCTCGCTCAAGCCAGCC
TTCGCCAAGGACGGCACGATCACCGCCGCCACCTCGTCGTCGATTTCCGACGGCGCGGCCGCCGTGGTCCTCGCCCGCCA
AAGCGTTGCCGCCGCCGCCGGTCTTGCGCCGGTCGCGCGGATCGTCGCCCTTGCCGCCCACGCGCAGGAACCGAAGGACT
TCACCACCGCTCCGGTCGGCGCGATCCAGAAGGTTCTCGACAAGGCCGGCTGGTCCATTGCCGACGTCGACCTGTTCGAG
GTGAACGAGGCGTTCGCCTGCGTCGCGATGTTCGCGATGCACGACCTCGGCATCCCGCACGAGAAGATCAACGTCCATGG
CGGCGCCACCGCGCTCGGTCACCCGATCGGCGCTTCGGGCACCCGCATCGTCACCACGCTGATCGCCGCGCTCAGGAAGC
ATGGCAAGACGAAGGGCGTCGCCAGCCTCTGCATCGGCGGCGGCGAGGCGACGGCCCTCGCGATCGAGCTGGTCTGA

Upstream 100 bases:

>100_bases
CTGCCCCGTTTGCTTTCGCTGCGCTGCAACACTACAAAGTTACAGGTCAGCCGGCGCCGCGCGGCGCCATCAGCACCGAT
TCCATAGGGACCGTCAAGTC

Downstream 100 bases:

>100_bases
AACCGTTGCATCTCCCCCGGCCCACGCGGTCGGGGGAAATCGCCCTTGCCCGGACGCCTCAGAGCTGGCGCCTCAGGGCT
GGCCCGCGCCCCACACGCTT

Product: Acetyl-CoA C-acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAQFSSSDPVVILSYARTPMGAMQGALAEVAATDLGATAVKAAVERAGVAPDKVERIYMGCVLPAGLGQAPARQAAIKAG
LPKSVQATTVNKVCGSGMQTIIMGAEALAAGSADLIVAGGMESMTNAPYLLKKHRSGARIGHDMAYDHMFLDGLEDAYDA
GRAMGTFAQDTANAYQLTREQQDAYSIESLRRAQAAVADGSFKDEVVAVTVKTRAGEVTVDTDEAPGKGRPDKIPSLKPA
FAKDGTITAATSSSISDGAAAVVLARQSVAAAAGLAPVARIVALAAHAQEPKDFTTAPVGAIQKVLDKAGWSIADVDLFE
VNEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASGTRIVTTLIAALRKHGKTKGVASLCIGGGEATALAIELV

Sequences:

>Translated_398_residues
MAQFSSSDPVVILSYARTPMGAMQGALAEVAATDLGATAVKAAVERAGVAPDKVERIYMGCVLPAGLGQAPARQAAIKAG
LPKSVQATTVNKVCGSGMQTIIMGAEALAAGSADLIVAGGMESMTNAPYLLKKHRSGARIGHDMAYDHMFLDGLEDAYDA
GRAMGTFAQDTANAYQLTREQQDAYSIESLRRAQAAVADGSFKDEVVAVTVKTRAGEVTVDTDEAPGKGRPDKIPSLKPA
FAKDGTITAATSSSISDGAAAVVLARQSVAAAAGLAPVARIVALAAHAQEPKDFTTAPVGAIQKVLDKAGWSIADVDLFE
VNEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASGTRIVTTLIAALRKHGKTKGVASLCIGGGEATALAIELV
>Mature_397_residues
AQFSSSDPVVILSYARTPMGAMQGALAEVAATDLGATAVKAAVERAGVAPDKVERIYMGCVLPAGLGQAPARQAAIKAGL
PKSVQATTVNKVCGSGMQTIIMGAEALAAGSADLIVAGGMESMTNAPYLLKKHRSGARIGHDMAYDHMFLDGLEDAYDAG
RAMGTFAQDTANAYQLTREQQDAYSIESLRRAQAAVADGSFKDEVVAVTVKTRAGEVTVDTDEAPGKGRPDKIPSLKPAF
AKDGTITAATSSSISDGAAAVVLARQSVAAAAGLAPVARIVALAAHAQEPKDFTTAPVGAIQKVLDKAGWSIADVDLFEV
NEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASGTRIVTTLIAALRKHGKTKGVASLCIGGGEATALAIELV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=45.29262086514, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=45.5012853470437, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=37.7551020408163, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4501853, Length=404, Percent_Identity=35.1485148514851, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=32.3040380047506, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI194353979, Length=400, Percent_Identity=27.25, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=48.9690721649485, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=44.3298969072165, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787663, Length=395, Percent_Identity=36.7088607594937, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=36.4532019704434, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=29.683698296837, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=45.5470737913486, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI25147385, Length=391, Percent_Identity=42.7109974424552, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=39.4871794871795, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17535917, Length=399, Percent_Identity=35.5889724310777, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=30.4038004750594, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=45.4773869346734, Blast_Score=325, Evalue=6e-90,
Organism=Saccharomyces cerevisiae, GI6322031, Length=392, Percent_Identity=33.1632653061224, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=47.0437017994859, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=44.0721649484536, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17648125, Length=398, Percent_Identity=34.9246231155779, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=28.8732394366197, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40675; Mature: 40544

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFSSSDPVVILSYARTPMGAMQGALAEVAATDLGATAVKAAVERAGVAPDKVERIYMG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
CVLPAGLGQAPARQAAIKAGLPKSVQATTVNKVCGSGMQTIIMGAEALAAGSADLIVAGG
HCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHCCCCCEEEECC
MESMTNAPYLLKKHRSGARIGHDMAYDHMFLDGLEDAYDAGRAMGTFAQDTANAYQLTRE
HHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQDAYSIESLRRAQAAVADGSFKDEVVAVTVKTRAGEVTVDTDEAPGKGRPDKIPSLKPA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCC
FAKDGTITAATSSSISDGAAAVVLARQSVAAAAGLAPVARIVALAAHAQEPKDFTTAPVG
CCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
AIQKVLDKAGWSIADVDLFEVNEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASG
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCHHCCCCCCCCC
TRIVTTLIAALRKHGKTKGVASLCIGGGEATALAIELV
HHHHHHHHHHHHHCCCCCCCHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
AQFSSSDPVVILSYARTPMGAMQGALAEVAATDLGATAVKAAVERAGVAPDKVERIYMG
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHH
CVLPAGLGQAPARQAAIKAGLPKSVQATTVNKVCGSGMQTIIMGAEALAAGSADLIVAGG
HCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHHHHHHHCCCCCEEEECC
MESMTNAPYLLKKHRSGARIGHDMAYDHMFLDGLEDAYDAGRAMGTFAQDTANAYQLTRE
HHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQDAYSIESLRRAQAAVADGSFKDEVVAVTVKTRAGEVTVDTDEAPGKGRPDKIPSLKPA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCC
FAKDGTITAATSSSISDGAAAVVLARQSVAAAAGLAPVARIVALAAHAQEPKDFTTAPVG
CCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
AIQKVLDKAGWSIADVDLFEVNEAFACVAMFAMHDLGIPHEKINVHGGATALGHPIGASG
HHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCHHCCCCCCCCC
TRIVTTLIAALRKHGKTKGVASLCIGGGEATALAIELV
HHHHHHHHHHHHHCCCCCCCHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]