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The map label for this gene is phoB [H]

Identifier: 87200295

GI number: 87200295

Start: 2422368

End: 2423057

Strand: Direct

Name: phoB [H]

Synonym: Saro_2281

Alternate gene names: 87200295

Gene position: 2422368-2423057 (Clockwise)

Preceding gene: 87200294

Following gene: 87200320

Centisome position: 68.01

GC content: 67.83

Gene sequence:

>690_bases
GTGCCTGCTCCCAAGCTGCTGCTGGTTGAAGACGACACGGCGCTTGCCGAACTGGTGGAATACCGGTTCCGCGGTGAAGG
TTACGACGTTCGCACCACCGACGACGGCGATGAAGCGCTGTTGCTTGCGGCCGAGGATACACCGGATCTGGTCCTGCTGG
ACTGGATGATCGGCGGAACCAGCGGGATCGAGGTCTGCCGCCGCCTGCGCCGGAACAAGGAAACCGCGCACGTCCCGATC
ATCATGCTCACCGCGCGCAGCGACGAGGACGACCGCATCCGCGGCCTCGAAATCGGCGCCGACGATTACGTGACCAAGCC
CTTCTCCCCGCGTGAGCTGATCGCCCGCGTCGGCGCAGTCCTGCGCCGGGTGCGCCCGGCGCTGGCGGGCGAGACGATTA
CCGTCGGCGACCTGTCGCTCGATCCCACCGCGCACCGCGTTTCGCGGCGCGGACAGCCGATGAAGGTCGGCCCCACCGAA
TTCCGCCTGCTCAAGCATTTCATGGAGCACCCCGGCCGGGTCTTCTCGCGCGGGCAACTGCTCGACGCGGTGTGGGGTAG
CGGCAGCGACATCGAACTGCGCACGGTGGACGTCCACATCCGCCGCCTTCGCCAGGCCATTGCCGTTCCGGGTGCCGCCG
ATCCGGTGCGGACGGTACGCTCTGCCGGATACGCGCTCGAAGGCGTCTGA

Upstream 100 bases:

>100_bases
AAATGTCGGAATCGGACCATCAGGGACGATCACCCGGGTGTTCGTCACACGACCGTAACATCGGTAGTGTCTTGGCAAAC
TGACTGTCATGGAGGTTTGC

Downstream 100 bases:

>100_bases
TCGCCCAGGCCGTAAACTCATAAAGCGCGCTCTTTATGAGTTTACGGCCTAGCGGTCGATCCGCTTGAGCACTGCGTTGT
AATCCAGCGCGCCGGCGGAC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPAPKLLLVEDDTALAELVEYRFRGEGYDVRTTDDGDEALLLAAEDTPDLVLLDWMIGGTSGIEVCRRLRRNKETAHVPI
IMLTARSDEDDRIRGLEIGADDYVTKPFSPRELIARVGAVLRRVRPALAGETITVGDLSLDPTAHRVSRRGQPMKVGPTE
FRLLKHFMEHPGRVFSRGQLLDAVWGSGSDIELRTVDVHIRRLRQAIAVPGAADPVRTVRSAGYALEGV

Sequences:

>Translated_229_residues
MPAPKLLLVEDDTALAELVEYRFRGEGYDVRTTDDGDEALLLAAEDTPDLVLLDWMIGGTSGIEVCRRLRRNKETAHVPI
IMLTARSDEDDRIRGLEIGADDYVTKPFSPRELIARVGAVLRRVRPALAGETITVGDLSLDPTAHRVSRRGQPMKVGPTE
FRLLKHFMEHPGRVFSRGQLLDAVWGSGSDIELRTVDVHIRRLRQAIAVPGAADPVRTVRSAGYALEGV
>Mature_228_residues
PAPKLLLVEDDTALAELVEYRFRGEGYDVRTTDDGDEALLLAAEDTPDLVLLDWMIGGTSGIEVCRRLRRNKETAHVPII
MLTARSDEDDRIRGLEIGADDYVTKPFSPRELIARVGAVLRRVRPALAGETITVGDLSLDPTAHRVSRRGQPMKVGPTEF
RLLKHFMEHPGRVFSRGQLLDAVWGSGSDIELRTVDVHIRRLRQAIAVPGAADPVRTVRSAGYALEGV

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=50.9009009009009, Blast_Score=230, Evalue=5e-62,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=41.9642857142857, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=39.0350877192982, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.5545851528384, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI87082012, Length=225, Percent_Identity=38.2222222222222, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=35.8649789029536, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=35.4260089686099, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787229, Length=233, Percent_Identity=36.0515021459227, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=37.3913043478261, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1786911, Length=214, Percent_Identity=34.5794392523364, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=30.4932735426009, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=32, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=36.6071428571429, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=32.3275862068966, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=30.2752293577982, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25312; Mature: 25181

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPKLLLVEDDTALAELVEYRFRGEGYDVRTTDDGDEALLLAAEDTPDLVLLDWMIGGT
CCCCCEEEEECCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCCEEEEEEECCCC
SGIEVCRRLRRNKETAHVPIIMLTARSDEDDRIRGLEIGADDYVTKPFSPRELIARVGAV
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRVRPALAGETITVGDLSLDPTAHRVSRRGQPMKVGPTEFRLLKHFMEHPGRVFSRGQL
HHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCHHHCCHH
LDAVWGSGSDIELRTVDVHIRRLRQAIAVPGAADPVRTVRSAGYALEGV
HHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEECCC
>Mature Secondary Structure 
PAPKLLLVEDDTALAELVEYRFRGEGYDVRTTDDGDEALLLAAEDTPDLVLLDWMIGGT
CCCCEEEEECCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEECCCCCEEEEEEECCCC
SGIEVCRRLRRNKETAHVPIIMLTARSDEDDRIRGLEIGADDYVTKPFSPRELIARVGAV
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
LRRVRPALAGETITVGDLSLDPTAHRVSRRGQPMKVGPTEFRLLKHFMEHPGRVFSRGQL
HHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCHHHCCHH
LDAVWGSGSDIELRTVDVHIRRLRQAIAVPGAADPVRTVRSAGYALEGV
HHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]