Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 87200316

GI number: 87200316

Start: 2442879

End: 2443424

Strand: Reverse

Name: nuoB

Synonym: Saro_2302

Alternate gene names: 87200316

Gene position: 2443424-2442879 (Counterclockwise)

Preceding gene: 87200317

Following gene: 87200315

Centisome position: 68.6

GC content: 65.2

Gene sequence:

>546_bases
ATGACCGCTTCTCCGCTCGTCACCGCAGATCAGGGTGCGGTTGCCGCGCCTGACCAGGCGTTTTTCGACAGCCTCAATGC
CGAGGTCAACGACAAGGGCTTCCTCGTCACTTCGACCGAGGAGCTGTTCCAGTGGGCGCGTACCGGCTCGCTGTGGTGGA
TGACCTTCGGGCTCGCCTGCTGCGCGGTCGAGATGATCCATGTGAACATGCCGCGCTACGACATGGAGCGCTTTGGCGTT
GCACCGCGCGCGTCGCCGCGCCAGTCGGACGTGATGATCGTGGCCGGCACGCTGTGCAACAAGATGGCCCCGGCGCTGCG
CAAGGTCTACGACCAGATGTCGGACCCCAAGTACGTGATCTCGATGGGGTCGTGCGCCAACGGCGGCGGCTACTATCACT
ACAGCTATTCGGTGGTGCGTGGCTGCGACCGCATCGTGCCCGTGGACATCTATGTCCCCGGCTGCCCGCCGACTGCCGAG
GCGCTGCTCTATGGCGTGATGCAGTTGCAGCGGAAGATCCGCCGCGCCGGCACGCTCGAGCGCTGA

Upstream 100 bases:

>100_bases
GCTGGGCCGGATGGACGACCATGATGGTGTTCCTCGGCGAACTGGTGATCGGCTTCATATACGCCTGGAAGAAGGGCGCG
CTGGATTGGGAATGAGCAAC

Downstream 100 bases:

>100_bases
GGGAGGCTGGATAATGACGGTACTCCACTCCGCTCCGCGCTGGTCCTCGAACGAGGGCGTGCTCGATACGCTGGTTGCTG
CGCTTGGCGACATGGTCGCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MTASPLVTADQGAVAAPDQAFFDSLNAEVNDKGFLVTSTEELFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGV
APRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAE
ALLYGVMQLQRKIRRAGTLER

Sequences:

>Translated_181_residues
MTASPLVTADQGAVAAPDQAFFDSLNAEVNDKGFLVTSTEELFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGV
APRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAE
ALLYGVMQLQRKIRRAGTLER
>Mature_180_residues
TASPLVTADQGAVAAPDQAFFDSLNAEVNDKGFLVTSTEELFQWARTGSLWWMTFGLACCAVEMIHVNMPRYDMERFGVA
PRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEA
LLYGVMQLQRKIRRAGTLER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI1788624, Length=152, Percent_Identity=50, Blast_Score=156, Evalue=9e-40,
Organism=Escherichia coli, GI1789074, Length=129, Percent_Identity=42.6356589147287, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=40.9836065573771, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=69.0140845070423, Blast_Score=225, Evalue=8e-60,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=76.5957446808511, Blast_Score=246, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=76.5957446808511, Blast_Score=246, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=75.886524822695, Blast_Score=239, Evalue=5e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_NOVAD (Q2G5Y4)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497573.1
- HSSP:   Q56218
- ProteinModelPortal:   Q2G5Y4
- SMR:   Q2G5Y4
- STRING:   Q2G5Y4
- GeneID:   3915647
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2302
- NMPDR:   fig|48935.1.peg.2905
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q2G5Y4
- ProtClustDB:   PRK06411
- BioCyc:   NARO279238:SARO_2302-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19978; Mature: 19847

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPLVTADQGAVAAPDQAFFDSLNAEVNDKGFLVTSTEELFQWARTGSLWWMTFGLAC
CCCCCCEECCCCCCCCCCHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHH
CAVEMIHVNMPRYDMERFGVAPRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVI
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
SMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRAGTLE
EECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
R
C
>Mature Secondary Structure 
TASPLVTADQGAVAAPDQAFFDSLNAEVNDKGFLVTSTEELFQWARTGSLWWMTFGLAC
CCCCCEECCCCCCCCCCHHHHHHCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHH
CAVEMIHVNMPRYDMERFGVAPRASPRQSDVMIVAGTLCNKMAPALRKVYDQMSDPKYVI
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEE
SMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGVMQLQRKIRRAGTLE
EECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA