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The map label for this gene is leuA [H]

Identifier: 87200281

GI number: 87200281

Start: 2406158

End: 2407828

Strand: Direct

Name: leuA [H]

Synonym: Saro_2267

Alternate gene names: 87200281

Gene position: 2406158-2407828 (Clockwise)

Preceding gene: 87200279

Following gene: 87200282

Centisome position: 67.56

GC content: 63.97

Gene sequence:

>1671_bases
ATGACCATGCTGAAGAACCCCTCGGCCAAGTACCGTCCCTTCCCGCAGATCGACCTGCCCGATCGCCAGTGGCCCAGCCG
CACGATCACCAAGGCCCCGCGCTGGCTCTCGACCGACATGCGCGACGGCAACCAGTCGCTGATCGATCCGATGGATGCCG
AAAAGAAGAGCCGTTTCTTCGATCTGCTGCTCAAGGTGGGCATCAAGGAAATCGAGGTCGGCTTCCCCTCTGCCGGCGCG
ACCGAGTACGATTTCATCCGCGGGCTGGTCGATTCCGGCCGCATTCCCGATGACGTCTTCGTCCAGGTCCTCACGCAGTC
CCGCGAGGACCTGATCAAGACGTCGTTCGAAAGCCTCGCCGGCGCGAAGCAGGCCATCGTCCACGTCTACAACGCGGTTT
CGCCGCTGTGGCGCCAGGTCGTGTTCGGCATGGAAAAGTCGGACGTGAAGGACATCGCCATCGCCGGTGCCAAGCACCTG
CGCGATCAGGCCGCTCGCTTCCCGCAGACCGACTGGCACTTCGAATACAGCCCGGAGACGTTCTCCACTGCCGAACTCGA
TTTCAGCATTGAATGCTGCGAGGCGGTCATGGAAATTCTCCAGCCGACGGTCGAAAAGCCGATCATCCTCAACCTGCCCG
CAACGGTCGAGGCGGCGACGGCCAACATCTATGCGGACCAGATCGAATACTTCTGCCGCAATCTGCCGGGCCGCGACCGC
GCGGTGATCTCGCTGCACACCCACAACGACCGCGGCACCGGCGTCGCCGCCGCCGAGCTCGGCCTGATGGCGGGCGCGGA
CCGCGTCGAGGGTTGCCTGTTCGGCAATGGCGAACGCACGGGCAATTGCTGCCTCGTTACCGTCGGCCTCAACATGTACA
CGCAAGGGGTCGATCCGGAGCTTGACTTCTCGGACATCGACGAAGTGATCCAGACGGTTGAATACTGCAACCAGCTCCCC
GTCCATCCGCGCCATCCCTATGGCGGCGAACTGGTCTTCACCGCGTTTTCCGGCAGCCACCAGGACGCCATCAAGAAGGG
CTTCGCCGCGCAGGAAAAGCGCAATGACGAACTCTGGTCGGTGCCCTACCTGCCGATCGACCCGGCCGATCTCGGCCGCA
GCTACGAAGCTGTGATCCGCGTCAATTCGCAATCCGGCAAGGGCGGCTTTGCCTGGGTCCTGGAACAGGACCAGGGCCTC
AAGCTGCCCAAGAAGATGCAGGCGCACTTCTCGCGCCACGTGCAGGAACTGGCCGACGAACTGGGCCGCGAACTGCAGGC
GGCAGATATCTGGGGCGTCTTCCGCAAGGCCTATCGCCTCGACGCGCCGCAGCATCTGCAACTGATCGACTACGAAGAAA
CGCGCGGTGCCGACGGGACGCGCATCTTCGCTGGCAAGATCGAGGTCGATGGCAAGGTCCAGTCCGTGTCCGGTCGTGGC
AATGGCCTGATTTCCTCGGTCGTGGCCACGCTGCGCGACGGCTTCGGCGTCGAGATCGACATAACCGACTATTCCGAACA
TGCGATGGGCGCCGGCAGCAACGCTCGCGCGGCCGCCTACGTCGAATGCAGGACCGCAGATGGCCGCACCATCTGGGGCG
TGGGGATCGATGAAGACGTCGCCACGGCCAGCGTCCGCGCCGTGCTCAGCGCGGCCAACGCGGTCGGGTGA

Upstream 100 bases:

>100_bases
CCCTTGGGCGTAAGCTGACGCGTCGGTCTGCCCCGGCGCGCACGGCGCCCAGGGGAACAGGAACCACGTAACGCAATCCA
GATCTTTCGAGTGCTTTTAC

Downstream 100 bases:

>100_bases
CCGCCGGCGGATGATTGTGGACGGGCGGCCTTGCGGGGCCGCCCGTTCAATCAAGCGATTAGAACCTGCTTGATACGGTC
GCGTCATGCGCCATAGTCCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTMLKNPSAKYRPFPQIDLPDRQWPSRTITKAPRWLSTDMRDGNQSLIDPMDAEKKSRFFDLLLKVGIKEIEVGFPSAGA
TEYDFIRGLVDSGRIPDDVFVQVLTQSREDLIKTSFESLAGAKQAIVHVYNAVSPLWRQVVFGMEKSDVKDIAIAGAKHL
RDQAARFPQTDWHFEYSPETFSTAELDFSIECCEAVMEILQPTVEKPIILNLPATVEAATANIYADQIEYFCRNLPGRDR
AVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTVGLNMYTQGVDPELDFSDIDEVIQTVEYCNQLP
VHPRHPYGGELVFTAFSGSHQDAIKKGFAAQEKRNDELWSVPYLPIDPADLGRSYEAVIRVNSQSGKGGFAWVLEQDQGL
KLPKKMQAHFSRHVQELADELGRELQAADIWGVFRKAYRLDAPQHLQLIDYEETRGADGTRIFAGKIEVDGKVQSVSGRG
NGLISSVVATLRDGFGVEIDITDYSEHAMGAGSNARAAAYVECRTADGRTIWGVGIDEDVATASVRAVLSAANAVG

Sequences:

>Translated_556_residues
MTMLKNPSAKYRPFPQIDLPDRQWPSRTITKAPRWLSTDMRDGNQSLIDPMDAEKKSRFFDLLLKVGIKEIEVGFPSAGA
TEYDFIRGLVDSGRIPDDVFVQVLTQSREDLIKTSFESLAGAKQAIVHVYNAVSPLWRQVVFGMEKSDVKDIAIAGAKHL
RDQAARFPQTDWHFEYSPETFSTAELDFSIECCEAVMEILQPTVEKPIILNLPATVEAATANIYADQIEYFCRNLPGRDR
AVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTVGLNMYTQGVDPELDFSDIDEVIQTVEYCNQLP
VHPRHPYGGELVFTAFSGSHQDAIKKGFAAQEKRNDELWSVPYLPIDPADLGRSYEAVIRVNSQSGKGGFAWVLEQDQGL
KLPKKMQAHFSRHVQELADELGRELQAADIWGVFRKAYRLDAPQHLQLIDYEETRGADGTRIFAGKIEVDGKVQSVSGRG
NGLISSVVATLRDGFGVEIDITDYSEHAMGAGSNARAAAYVECRTADGRTIWGVGIDEDVATASVRAVLSAANAVG
>Mature_555_residues
TMLKNPSAKYRPFPQIDLPDRQWPSRTITKAPRWLSTDMRDGNQSLIDPMDAEKKSRFFDLLLKVGIKEIEVGFPSAGAT
EYDFIRGLVDSGRIPDDVFVQVLTQSREDLIKTSFESLAGAKQAIVHVYNAVSPLWRQVVFGMEKSDVKDIAIAGAKHLR
DQAARFPQTDWHFEYSPETFSTAELDFSIECCEAVMEILQPTVEKPIILNLPATVEAATANIYADQIEYFCRNLPGRDRA
VISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTVGLNMYTQGVDPELDFSDIDEVIQTVEYCNQLPV
HPRHPYGGELVFTAFSGSHQDAIKKGFAAQEKRNDELWSVPYLPIDPADLGRSYEAVIRVNSQSGKGGFAWVLEQDQGLK
LPKKMQAHFSRHVQELADELGRELQAADIWGVFRKAYRLDAPQHLQLIDYEETRGADGTRIFAGKIEVDGKVQSVSGRGN
GLISSVVATLRDGFGVEIDITDYSEHAMGAGSNARAAAYVECRTADGRTIWGVGIDEDVATASVRAVLSAANAVG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=522, Percent_Identity=23.9463601532567, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=51.4991181657848, Blast_Score=601, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6324682, Length=568, Percent_Identity=50.1760563380282, Blast_Score=592, Evalue=1e-170,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61208; Mature: 61076

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLKNPSAKYRPFPQIDLPDRQWPSRTITKAPRWLSTDMRDGNQSLIDPMDAEKKSRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCHHHCCCCCHHHHHHHH
DLLLKVGIKEIEVGFPSAGATEYDFIRGLVDSGRIPDDVFVQVLTQSREDLIKTSFESLA
HHHHHHCHHHEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GAKQAIVHVYNAVSPLWRQVVFGMEKSDVKDIAIAGAKHLRDQAARFPQTDWHFEYSPET
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FSTAELDFSIECCEAVMEILQPTVEKPIILNLPATVEAATANIYADQIEYFCRNLPGRDR
CCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTVGLNMYTQGVDPE
EEEEEEECCCCCCCHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECCCHHCCCCCCC
LDFSDIDEVIQTVEYCNQLPVHPRHPYGGELVFTAFSGSHQDAIKKGFAAQEKRNDELWS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHCCCCCEE
VPYLPIDPADLGRSYEAVIRVNSQSGKGGFAWVLEQDQGLKLPKKMQAHFSRHVQELADE
CCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LGRELQAADIWGVFRKAYRLDAPQHLQLIDYEETRGADGTRIFAGKIEVDGKVQSVSGRG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCEEEEEEEEECCEEEEECCCC
NGLISSVVATLRDGFGVEIDITDYSEHAMGAGSNARAAAYVECRTADGRTIWGVGIDEDV
CHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCEEEEEEEEECCCCEEEECCCCCHH
ATASVRAVLSAANAVG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMLKNPSAKYRPFPQIDLPDRQWPSRTITKAPRWLSTDMRDGNQSLIDPMDAEKKSRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHCCCCCCCCHHHCCCCCHHHHHHHH
DLLLKVGIKEIEVGFPSAGATEYDFIRGLVDSGRIPDDVFVQVLTQSREDLIKTSFESLA
HHHHHHCHHHEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GAKQAIVHVYNAVSPLWRQVVFGMEKSDVKDIAIAGAKHLRDQAARFPQTDWHFEYSPET
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FSTAELDFSIECCEAVMEILQPTVEKPIILNLPATVEAATANIYADQIEYFCRNLPGRDR
CCEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AVISLHTHNDRGTGVAAAELGLMAGADRVEGCLFGNGERTGNCCLVTVGLNMYTQGVDPE
EEEEEEECCCCCCCHHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECCCHHCCCCCCC
LDFSDIDEVIQTVEYCNQLPVHPRHPYGGELVFTAFSGSHQDAIKKGFAAQEKRNDELWS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCHHHHCCCCCEE
VPYLPIDPADLGRSYEAVIRVNSQSGKGGFAWVLEQDQGLKLPKKMQAHFSRHVQELADE
CCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
LGRELQAADIWGVFRKAYRLDAPQHLQLIDYEETRGADGTRIFAGKIEVDGKVQSVSGRG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCCCCCCEEEEEEEEECCEEEEECCCC
NGLISSVVATLRDGFGVEIDITDYSEHAMGAGSNARAAAYVECRTADGRTIWGVGIDEDV
CHHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCEEEEEEEEECCCCEEEECCCCCHH
ATASVRAVLSAANAVG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA