Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 87200275

GI number: 87200275

Start: 2400108

End: 2401856

Strand: Direct

Name: ilvI [H]

Synonym: Saro_2261

Alternate gene names: 87200275

Gene position: 2400108-2401856 (Clockwise)

Preceding gene: 87200274

Following gene: 87200276

Centisome position: 67.39

GC content: 65.81

Gene sequence:

>1749_bases
GTGACAACCGAGCGCAGCGGCGCCGAGATCCTGGTCGAAAGCCTCGTCGCAAAGGGGGTCGAGTTCGTGTTCGGCTATCC
GGGCGGCGCGGTGCTCCCGATCTACGATGCGCTGTTCGGCGATGAACGCCTCCGCCACATCCTAGTCCGCCACGAAGCCG
GCGCTGCCCACGCCGCCGAAGGCTACGCGCGCGCCACCGGCAAGCCCGGCGTCGTGCTCGTCACCTCGGGCCCGGGCGCG
ACCAACGCCGTCACCGGCATTGCCGATGCGTTCATGGATTCGATTCCGATGGTCGTCATCACCGGCCAGGTCCCCACGGC
CCTGATCGGTTCGGATGCGTTCCAGGAAGCCGACACGATCGGCATCACGCGCCACTGCACGAAGCACAACTACCTCGTGA
AGGACCCGTCGCAGCTGGCGGCGACCATCGACGAAGCGTTCCGCATCGCCACCGAGGGCCGCCCCGGCCCGGTCGTGATC
GACATCCCCAAGGATGTGCAGATCGCGACGGCCGCGTGGAGCAGCGCCGCCCCGCAGCAGCGCAACCGCTATGCTCCCCA
GACCGAGGGCGGCGCGAAGGAGATTGCCGAAGCGATCGAACTGATCGCCAAGGCCAAGGCGCCGGTCTTCTACACGGGCG
GCGGCGTGATCAATGCCGGCCCGGAAGCGGCCCGCCTGCTGCGCGAACTCCAGTCGCTGACCGGCGCGCCCGTGACCTCC
ACGCTGATGGGCCTTGGCGCGTTTCCGGCGGACCACCCGGCATGGCTCGGCATGCTCGGCATGCACGGCACCTACGAAGC
CAACATGACGATGAACCGCGCCGACCTCGTCGTCTGCATCGGCGCCCGCTTCGACGACCGCGTCACCGGCCGGCTCGATG
CCTTCTCGCCCAACAGCAAGAAGATCCACATCGACATCGACCGGGCCTCGATCAACAAGACCGTCCAGGTCGACCTTCCG
GTCATCGGCGATTGCGGCAAGGTCCTTGCCCAGCTCATCGAAGCATGGCTCGAGCGCGGCTACCAGAAGCAGGACCTGAC
TGAATGGAACGCCCGCATCGATGGCTGGCGTGCCCGGCAGAGCCTCGCCTTCCCGCGTCGCGGCAAGGAAATCATGCCCC
AGCTGGCGATCGAACGTCTCTATGAGCTGACCAGGCACAAGGAGCCGATCATCAGCACCGAAGTCGGCCAGCACCAGATG
TGGGCTGCCCAGCACTTCCACTTCATGAAGCCGAACAAGTGGCTGACCTCGGGCGGCCTCGGCACGATGGGCTATGGCCT
TCCCGCCGCGATCGGTGCACAGGCCGGCTTCCCCGATGCGCTGGTCATCGACATCGCGGGCGATGCTTCGATCCAGATGA
ACATCCAGGAACTCGGCACGGCCACCCAGTACCGCCTTCCGGTCAAGGTCTTCGTGCTGAACAACGAATGGATGGGCATG
GTCCGCCAGTGGCAGGAACTCACCTACGAGAGCCGCTACTCGAACTCCTACTCGGACAGCCTGCCCGATTTCGTGAAGCT
CGCCGAAGCCTACGGCTGGAAGGGCATCCGCATCACCGACGAAAGCGAGCTGGACGCCGGCATCCAGGCGATGATCGATC
ATGACGGCCCGGTCTTCGTCGACTGCCTCGTCGCCAAGGAAGCGAATTGCTTCCCGATGATCCCGTCGGGGGCTGCGCAC
ACCGACATGATCCTCTACGGGGACGAAGTCGCCGGCACGATGGACGACGAAGCCAAGGCGCTGGTCTGA

Upstream 100 bases:

>100_bases
GACGGCTCGGCGCAGCATGCCTGCGCCGAGCCGAAATTTTTTGTTTTTTGCAGGTGCGGTCGGGGCTATCGGGCCACGAC
GCGAAGAAGGATGAAGTAAC

Downstream 100 bases:

>100_bases
AGGACACCGGAACAATGATGCACATCGCTCAGGAGGCGCAGGAGCGCCACGTCCTCACCATCACTGTCGACAACGAGGCA
GGCATCCTCGCCAAGATCGC

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MTTERSGAEILVESLVAKGVEFVFGYPGGAVLPIYDALFGDERLRHILVRHEAGAAHAAEGYARATGKPGVVLVTSGPGA
TNAVTGIADAFMDSIPMVVITGQVPTALIGSDAFQEADTIGITRHCTKHNYLVKDPSQLAATIDEAFRIATEGRPGPVVI
DIPKDVQIATAAWSSAAPQQRNRYAPQTEGGAKEIAEAIELIAKAKAPVFYTGGGVINAGPEAARLLRELQSLTGAPVTS
TLMGLGAFPADHPAWLGMLGMHGTYEANMTMNRADLVVCIGARFDDRVTGRLDAFSPNSKKIHIDIDRASINKTVQVDLP
VIGDCGKVLAQLIEAWLERGYQKQDLTEWNARIDGWRARQSLAFPRRGKEIMPQLAIERLYELTRHKEPIISTEVGQHQM
WAAQHFHFMKPNKWLTSGGLGTMGYGLPAAIGAQAGFPDALVIDIAGDASIQMNIQELGTATQYRLPVKVFVLNNEWMGM
VRQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIRITDESELDAGIQAMIDHDGPVFVDCLVAKEANCFPMIPSGAAH
TDMILYGDEVAGTMDDEAKALV

Sequences:

>Translated_582_residues
MTTERSGAEILVESLVAKGVEFVFGYPGGAVLPIYDALFGDERLRHILVRHEAGAAHAAEGYARATGKPGVVLVTSGPGA
TNAVTGIADAFMDSIPMVVITGQVPTALIGSDAFQEADTIGITRHCTKHNYLVKDPSQLAATIDEAFRIATEGRPGPVVI
DIPKDVQIATAAWSSAAPQQRNRYAPQTEGGAKEIAEAIELIAKAKAPVFYTGGGVINAGPEAARLLRELQSLTGAPVTS
TLMGLGAFPADHPAWLGMLGMHGTYEANMTMNRADLVVCIGARFDDRVTGRLDAFSPNSKKIHIDIDRASINKTVQVDLP
VIGDCGKVLAQLIEAWLERGYQKQDLTEWNARIDGWRARQSLAFPRRGKEIMPQLAIERLYELTRHKEPIISTEVGQHQM
WAAQHFHFMKPNKWLTSGGLGTMGYGLPAAIGAQAGFPDALVIDIAGDASIQMNIQELGTATQYRLPVKVFVLNNEWMGM
VRQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIRITDESELDAGIQAMIDHDGPVFVDCLVAKEANCFPMIPSGAAH
TDMILYGDEVAGTMDDEAKALV
>Mature_581_residues
TTERSGAEILVESLVAKGVEFVFGYPGGAVLPIYDALFGDERLRHILVRHEAGAAHAAEGYARATGKPGVVLVTSGPGAT
NAVTGIADAFMDSIPMVVITGQVPTALIGSDAFQEADTIGITRHCTKHNYLVKDPSQLAATIDEAFRIATEGRPGPVVID
IPKDVQIATAAWSSAAPQQRNRYAPQTEGGAKEIAEAIELIAKAKAPVFYTGGGVINAGPEAARLLRELQSLTGAPVTST
LMGLGAFPADHPAWLGMLGMHGTYEANMTMNRADLVVCIGARFDDRVTGRLDAFSPNSKKIHIDIDRASINKTVQVDLPV
IGDCGKVLAQLIEAWLERGYQKQDLTEWNARIDGWRARQSLAFPRRGKEIMPQLAIERLYELTRHKEPIISTEVGQHQMW
AAQHFHFMKPNKWLTSGGLGTMGYGLPAAIGAQAGFPDALVIDIAGDASIQMNIQELGTATQYRLPVKVFVLNNEWMGMV
RQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIRITDESELDAGIQAMIDHDGPVFVDCLVAKEANCFPMIPSGAAHT
DMILYGDEVAGTMDDEAKALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=24.468085106383, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI21361361, Length=594, Percent_Identity=26.7676767676768, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=50.0875656742557, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=44.4642857142857, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=33.1531531531532, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=29.1592128801431, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=25.3164556962025, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=575, Percent_Identity=27.1304347826087, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=575, Percent_Identity=26.9565217391304, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=575, Percent_Identity=26.9565217391304, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=47.1890971039182, Blast_Score=524, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.8484848484848, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320123, Length=540, Percent_Identity=23.5185185185185, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=501, Percent_Identity=24.5508982035928, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=325, Percent_Identity=26.7692307692308, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=23.4343434343434, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=24.5250431778929, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63005; Mature: 62873

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTERSGAEILVESLVAKGVEFVFGYPGGAVLPIYDALFGDERLRHILVRHEAGAAHAAE
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCHHHC
GYARATGKPGVVLVTSGPGATNAVTGIADAFMDSIPMVVITGQVPTALIGSDAFQEADTI
CHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHC
GITRHCTKHNYLVKDPSQLAATIDEAFRIATEGRPGPVVIDIPKDVQIATAAWSSAAPQQ
CHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCHH
RNRYAPQTEGGAKEIAEAIELIAKAKAPVFYTGGGVINAGPEAARLLRELQSLTGAPVTS
HHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHCCCCHHH
TLMGLGAFPADHPAWLGMLGMHGTYEANMTMNRADLVVCIGARFDDRVTGRLDAFSPNSK
HHHHCCCCCCCCCHHHHHHCCCCEEECCEEECCCCEEEEECCCCCCCCCCEEECCCCCCC
KIHIDIDRASINKTVQVDLPVIGDCGKVLAQLIEAWLERGYQKQDLTEWNARIDGWRARQ
EEEEEEECCCCCCEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
SLAFPRRGKEIMPQLAIERLYELTRHKEPIISTEVGQHQMWAAQHFHFMKPNKWLTSGGL
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCEEECCCCEEECCCC
GTMGYGLPAAIGAQAGFPDALVIDIAGDASIQMNIQELGTATQYRLPVKVFVLNNEWMGM
CCCCCCCCHHHCCCCCCCCEEEEEECCCCEEEEEHHHHCCCCEEECEEEEEEECCHHHHH
VRQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIRITDESELDAGIQAMIDHDGPVFV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHCCCHHEEECCCCCEEE
DCLVAKEANCFPMIPSGAAHTDMILYGDEVAGTMDDEAKALV
EEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHCCC
>Mature Secondary Structure 
TTERSGAEILVESLVAKGVEFVFGYPGGAVLPIYDALFGDERLRHILVRHEAGAAHAAE
CCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCHHHC
GYARATGKPGVVLVTSGPGATNAVTGIADAFMDSIPMVVITGQVPTALIGSDAFQEADTI
CHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHC
GITRHCTKHNYLVKDPSQLAATIDEAFRIATEGRPGPVVIDIPKDVQIATAAWSSAAPQQ
CHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCHH
RNRYAPQTEGGAKEIAEAIELIAKAKAPVFYTGGGVINAGPEAARLLRELQSLTGAPVTS
HHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHCCCCHHH
TLMGLGAFPADHPAWLGMLGMHGTYEANMTMNRADLVVCIGARFDDRVTGRLDAFSPNSK
HHHHCCCCCCCCCHHHHHHCCCCEEECCEEECCCCEEEEECCCCCCCCCCEEECCCCCCC
KIHIDIDRASINKTVQVDLPVIGDCGKVLAQLIEAWLERGYQKQDLTEWNARIDGWRARQ
EEEEEEECCCCCCEEEEECCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
SLAFPRRGKEIMPQLAIERLYELTRHKEPIISTEVGQHQMWAAQHFHFMKPNKWLTSGGL
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHCEEECCCCEEECCCC
GTMGYGLPAAIGAQAGFPDALVIDIAGDASIQMNIQELGTATQYRLPVKVFVLNNEWMGM
CCCCCCCCHHHCCCCCCCCEEEEEECCCCEEEEEHHHHCCCCEEECEEEEEEECCHHHHH
VRQWQELTYESRYSNSYSDSLPDFVKLAEAYGWKGIRITDESELDAGIQAMIDHDGPVFV
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHCCCHHEEECCCCCEEE
DCLVAKEANCFPMIPSGAAHTDMILYGDEVAGTMDDEAKALV
EEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]