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The map label for this gene is miaA

Identifier: 87200274

GI number: 87200274

Start: 2399076

End: 2399921

Strand: Direct

Name: miaA

Synonym: Saro_2260

Alternate gene names: 87200274

Gene position: 2399076-2399921 (Clockwise)

Preceding gene: 87200272

Following gene: 87200275

Centisome position: 67.36

GC content: 68.32

Gene sequence:

>846_bases
ATGGATCGGGGGCAGGACGCGGTCATCGTCAATGCCGACAGCGCGCAAGTCTATGCCGATCTCCGCGTCCTCAGCGCGCG
GCCTTCGGACGAAGAGATGCGCGGCGTGCCGCACGTGCTGTTCGGCGCGTGGGACGGGGCCCGGGCCTGCTCTGCCGCGG
ACTGGGCAGCCGCAGCCCGGCGCGAGATCGCCGCCGCACATCAGCGCGGCGCGCTTCCGATCCTCGTCGGCGGCACCGGC
CTCTATATCCGGACCCTGCTCGACGGCATCGCTCCGGTCCCCGAGATCGACCCGCAGGTCCGCGAAGAGGTCCGCGCGAT
GCCGCTGGAGGCCGCCTACGCGGAGCTCGAGAAATCCGACCCCGAACGCGCCCGCAAGCTCGCCCCCGCCGATGCGCAGC
GCATTACCCGTGCGCTCGAAGTCATGCGCTCCACCGGGCGTCCCCTGGCCTACTGGCAGCAGCAGCTCAGCGGCGGCATC
GGCAACGATGTCGCGCTTGCCCCGCTGATCCTCCTGCCGGAGCGCCAGTGGCTCTACAGACGTTGCGACTTGCGCTTCGA
ACTGATGTGGGACGGCGGCGCGCTCGAAGAAGTAGAGGCGCTGCTCGCCCGCGATCTGCCCGATTCCCTGCCCGTCATGC
GCGCCATCGGCGTGCCCGAGATCGCGGCCTTCCTGCGCGGCGATCTGTCCCGCGACGCGGCCATCGCATCGGGTCAGCAG
GCGACCCGGAACTACGCCAAGCGGCAGTACACATGGCTCAGGCACCAGAATCCGGGCGATTGGCCGCGAATGGAGTATGA
TAATTCCATTGACAGCGCCAAGATCGCAAGTTTATTACGCCTATAG

Upstream 100 bases:

>100_bases
TAACGTGAAGGCAGCGGCCGAACGGCCAAGGTTGGCGCTAATCGCAGGGCCGACGGCCAGCGGCAAGAGCGATTTGGCCG
TGAACCTCGCTTTGCGCGCC

Downstream 100 bases:

>100_bases
CCGCTTGACACGTCATTTTATGTCAAGTAGCAGGCCGTCAACTGCTCGATCCACGTCTCGTGTCGATCGGGTGCCCGATA
GGGAGAGACGGCTCGGCGCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDRGQDAVIVNADSAQVYADLRVLSARPSDEEMRGVPHVLFGAWDGARACSAADWAAAARREIAAAHQRGALPILVGGTG
LYIRTLLDGIAPVPEIDPQVREEVRAMPLEAAYAELEKSDPERARKLAPADAQRITRALEVMRSTGRPLAYWQQQLSGGI
GNDVALAPLILLPERQWLYRRCDLRFELMWDGGALEEVEALLARDLPDSLPVMRAIGVPEIAAFLRGDLSRDAAIASGQQ
ATRNYAKRQYTWLRHQNPGDWPRMEYDNSIDSAKIASLLRL

Sequences:

>Translated_281_residues
MDRGQDAVIVNADSAQVYADLRVLSARPSDEEMRGVPHVLFGAWDGARACSAADWAAAARREIAAAHQRGALPILVGGTG
LYIRTLLDGIAPVPEIDPQVREEVRAMPLEAAYAELEKSDPERARKLAPADAQRITRALEVMRSTGRPLAYWQQQLSGGI
GNDVALAPLILLPERQWLYRRCDLRFELMWDGGALEEVEALLARDLPDSLPVMRAIGVPEIAAFLRGDLSRDAAIASGQQ
ATRNYAKRQYTWLRHQNPGDWPRMEYDNSIDSAKIASLLRL
>Mature_281_residues
MDRGQDAVIVNADSAQVYADLRVLSARPSDEEMRGVPHVLFGAWDGARACSAADWAAAARREIAAAHQRGALPILVGGTG
LYIRTLLDGIAPVPEIDPQVREEVRAMPLEAAYAELEKSDPERARKLAPADAQRITRALEVMRSTGRPLAYWQQQLSGGI
GNDVALAPLILLPERQWLYRRCDLRFELMWDGGALEEVEALLARDLPDSLPVMRAIGVPEIAAFLRGDLSRDAAIASGQQ
ATRNYAKRQYTWLRHQNPGDWPRMEYDNSIDSAKIASLLRL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=282, Percent_Identity=25.886524822695, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1790613, Length=254, Percent_Identity=37.007874015748, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6324848, Length=262, Percent_Identity=26.7175572519084, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24649830, Length=250, Percent_Identity=26.4, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_NOVAD (Q2G626)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497531.1
- ProteinModelPortal:   Q2G626
- SMR:   Q2G626
- STRING:   Q2G626
- GeneID:   3916576
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2260
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   NARO279238:SARO_2260-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 31074; Mature: 31074

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRGQDAVIVNADSAQVYADLRVLSARPSDEEMRGVPHVLFGAWDGARACSAADWAAAAR
CCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCHHCCHHHHHHHHH
REIAAAHQRGALPILVGGTGLYIRTLLDGIAPVPEIDPQVREEVRAMPLEAAYAELEKSD
HHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCC
PERARKLAPADAQRITRALEVMRSTGRPLAYWQQQLSGGIGNDVALAPLILLPERQWLYR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHH
RCDLRFELMWDGGALEEVEALLARDLPDSLPVMRAIGVPEIAAFLRGDLSRDAAIASGQQ
HCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCHH
ATRNYAKRQYTWLRHQNPGDWPRMEYDNSIDSAKIASLLRL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDRGQDAVIVNADSAQVYADLRVLSARPSDEEMRGVPHVLFGAWDGARACSAADWAAAAR
CCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCHHCCHHHHHHHHH
REIAAAHQRGALPILVGGTGLYIRTLLDGIAPVPEIDPQVREEVRAMPLEAAYAELEKSD
HHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCC
PERARKLAPADAQRITRALEVMRSTGRPLAYWQQQLSGGIGNDVALAPLILLPERQWLYR
HHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHH
RCDLRFELMWDGGALEEVEALLARDLPDSLPVMRAIGVPEIAAFLRGDLSRDAAIASGQQ
HCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCHH
ATRNYAKRQYTWLRHQNPGDWPRMEYDNSIDSAKIASLLRL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA