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The map label for this gene is nylB' [H]

Identifier: 87200041

GI number: 87200041

Start: 2158544

End: 2159749

Strand: Reverse

Name: nylB' [H]

Synonym: Saro_2025

Alternate gene names: 87200041

Gene position: 2159749-2158544 (Counterclockwise)

Preceding gene: 87200042

Following gene: 87200035

Centisome position: 60.64

GC content: 66.58

Gene sequence:

>1206_bases
ATGCGGAAGCGGTGGGTTGCGCTGGCTGCGGTGGTATTGGCGGGCGGAGCGGCATTTGCTTCGCTCGACAAGGAAACGCG
CGGGGTGCTGTTGGCCATGCCGACCAACGCCGACGTGCTTTCCTGGGAACAGGGCCAGCGCGATGCGGCATTCAGGGCGA
TGGACCGCCTGCCGTTCCTGGCCAAGGCCAACACGATCGCTCCTGCCGCCGAGCCGCTGCCGCTTCCGTCCGGCAAGCCG
CTCGCCGTGCCGGGGATCGATGCCTACATGGCCCGGCAGCGCACCGCAGGGCTGGTGATCGTGCAGGACGGCAAGGTACG
GCTTGAGCGCTATGGCTTGGGCTTCGATGCCAAGGGGCGGTGGACGAGCTTTTCGGTGGCCAAGTCGTTCACCTCGACGC
TGGTCGGCGCGGCGGTGAAGGATGGCGCGATCCGCAGCCTCGACGACAAAGTCAGCCAGTACATTCCCGGCCTGAAGGGC
AGCGCATATGACGACGTGAGCGTGCGCCAGCTCCTTACCATGTCATCGGGCGTGCGATGGAACGAGGACTATGAGGACCC
TAGGTCGGACGTCGCCGAGTTCAACCGCGCCAAGCCCGAAGACGGGCTGGATGCGACGGTGAGCTATATGCGCAAGTTGC
CCCGTGCGCATCCGCCGGGAGAGGTCTGGCACTACAACACGGGCGAAACCAACCTGATCGGCGTGCTGGTTACTTCGGCG
ACGGGCAAGTCGCTGGCGGCCTATCTGCGGGAGAAGGTGTGGCAGCCGGCGGGCATGGAGGCCGAGGCAACCTGGTTGCT
GGGCAAGACCGGCCACGAGATCGGCGGATGTTGCCTGCAGGCGGCGACGCGCGACTATGCGCGCTTCGGGCTGCTCGTGC
TGGCGAACGGCAAGGGCCGGGACGGACGGCAGGTGGTGCCGGAGGACTGGTTCGGTCAGGCGACGACGAAGCAAAAGGAC
ATCGGCAAGCCGGGACGCGGCTATGGGTTCCAGTGGTGGACCAACGATGACGGCACCTTTGCCGCGCAGGGCATTTTCGG
CCAGGGCATCTTTATCGACCCGAAGCGCAAGCTGGTGATCGCGAGCAATTCCGATTGGCCGCGCGCCAGCGCAGGGCCGC
AACCCGAGGAGCGCGAGGCGTTCTACCGGCAGGTCCAGCGACTGCTCGACGCGGAGCAGGGGGCCGGACCGGTGCGCCTG
AACTAA

Upstream 100 bases:

>100_bases
CGGACATTGGTGGCTTGCACCGGTTATCGAGGCCGGCTTCCCGCTTGCAGGCTTGTCGCGCGTGGTTATTGTCCCTCGTG
TAAGCAAGCGGGAGGGGCGG

Downstream 100 bases:

>100_bases
CCGAACAACAGTCCGCGCGTTGCCGCTTCGACGTCGGCCTGGCGCATCAGGCTTTCGCCGACGAGAAACGTGCGGGCGCC
GCACTTTTCGAGGCGAAGCA

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII' [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRKRWVALAAVVLAGGAAFASLDKETRGVLLAMPTNADVLSWEQGQRDAAFRAMDRLPFLAKANTIAPAAEPLPLPSGKP
LAVPGIDAYMARQRTAGLVIVQDGKVRLERYGLGFDAKGRWTSFSVAKSFTSTLVGAAVKDGAIRSLDDKVSQYIPGLKG
SAYDDVSVRQLLTMSSGVRWNEDYEDPRSDVAEFNRAKPEDGLDATVSYMRKLPRAHPPGEVWHYNTGETNLIGVLVTSA
TGKSLAAYLREKVWQPAGMEAEATWLLGKTGHEIGGCCLQAATRDYARFGLLVLANGKGRDGRQVVPEDWFGQATTKQKD
IGKPGRGYGFQWWTNDDGTFAAQGIFGQGIFIDPKRKLVIASNSDWPRASAGPQPEEREAFYRQVQRLLDAEQGAGPVRL
N

Sequences:

>Translated_401_residues
MRKRWVALAAVVLAGGAAFASLDKETRGVLLAMPTNADVLSWEQGQRDAAFRAMDRLPFLAKANTIAPAAEPLPLPSGKP
LAVPGIDAYMARQRTAGLVIVQDGKVRLERYGLGFDAKGRWTSFSVAKSFTSTLVGAAVKDGAIRSLDDKVSQYIPGLKG
SAYDDVSVRQLLTMSSGVRWNEDYEDPRSDVAEFNRAKPEDGLDATVSYMRKLPRAHPPGEVWHYNTGETNLIGVLVTSA
TGKSLAAYLREKVWQPAGMEAEATWLLGKTGHEIGGCCLQAATRDYARFGLLVLANGKGRDGRQVVPEDWFGQATTKQKD
IGKPGRGYGFQWWTNDDGTFAAQGIFGQGIFIDPKRKLVIASNSDWPRASAGPQPEEREAFYRQVQRLLDAEQGAGPVRL
N
>Mature_401_residues
MRKRWVALAAVVLAGGAAFASLDKETRGVLLAMPTNADVLSWEQGQRDAAFRAMDRLPFLAKANTIAPAAEPLPLPSGKP
LAVPGIDAYMARQRTAGLVIVQDGKVRLERYGLGFDAKGRWTSFSVAKSFTSTLVGAAVKDGAIRSLDDKVSQYIPGLKG
SAYDDVSVRQLLTMSSGVRWNEDYEDPRSDVAEFNRAKPEDGLDATVSYMRKLPRAHPPGEVWHYNTGETNLIGVLVTSA
TGKSLAAYLREKVWQPAGMEAEATWLLGKTGHEIGGCCLQAATRDYARFGLLVLANGKGRDGRQVVPEDWFGQATTKQKD
IGKPGRGYGFQWWTNDDGTFAAQGIFGQGIFIDPKRKLVIASNSDWPRASAGPQPEEREAFYRQVQRLLDAEQGAGPVRL
N

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 43673; Mature: 43673

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRWVALAAVVLAGGAAFASLDKETRGVLLAMPTNADVLSWEQGQRDAAFRAMDRLPFL
CCCHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCH
AKANTIAPAAEPLPLPSGKPLAVPGIDAYMARQRTAGLVIVQDGKVRLERYGLGFDAKGR
HCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCC
WTSFSVAKSFTSTLVGAAVKDGAIRSLDDKVSQYIPGLKGSAYDDVSVRQLLTMSSGVRW
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
NEDYEDPRSDVAEFNRAKPEDGLDATVSYMRKLPRAHPPGEVWHYNTGETNLIGVLVTSA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECC
TGKSLAAYLREKVWQPAGMEAEATWLLGKTGHEIGGCCLQAATRDYARFGLLVLANGKGR
CCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCC
DGRQVVPEDWFGQATTKQKDIGKPGRGYGFQWWTNDDGTFAAQGIFGQGIFIDPKRKLVI
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCEEECCCCEEEE
ASNSDWPRASAGPQPEEREAFYRQVQRLLDAEQGAGPVRLN
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRKRWVALAAVVLAGGAAFASLDKETRGVLLAMPTNADVLSWEQGQRDAAFRAMDRLPFL
CCCHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCH
AKANTIAPAAEPLPLPSGKPLAVPGIDAYMARQRTAGLVIVQDGKVRLERYGLGFDAKGR
HCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCC
WTSFSVAKSFTSTLVGAAVKDGAIRSLDDKVSQYIPGLKGSAYDDVSVRQLLTMSSGVRW
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
NEDYEDPRSDVAEFNRAKPEDGLDATVSYMRKLPRAHPPGEVWHYNTGETNLIGVLVTSA
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECC
TGKSLAAYLREKVWQPAGMEAEATWLLGKTGHEIGGCCLQAATRDYARFGLLVLANGKGR
CCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCC
DGRQVVPEDWFGQATTKQKDIGKPGRGYGFQWWTNDDGTFAAQGIFGQGIFIDPKRKLVI
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCEEECCCCEEEE
ASNSDWPRASAGPQPEEREAFYRQVQRLLDAEQGAGPVRLN
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 1879421 [H]