Click here to switch to the map view.

The map label for this gene is tpiA [H]

Identifier: 87200035

GI number: 87200035

Start: 2151568

End: 2152338

Strand: Reverse

Name: tpiA [H]

Synonym: Saro_2019

Alternate gene names: 87200035

Gene position: 2152338-2151568 (Counterclockwise)

Preceding gene: 87200041

Following gene: 87200034

Centisome position: 60.43

GC content: 69.26

Gene sequence:

>771_bases
ATGTCGCATCGTCCTTACATTGTCGGAAACTGGAAGATGAACGGGAGCCGCGCCATGCTGGCCGAAGCCCGCGCGATCGA
CCGGGCGGCGGGGCGCTATCCCGACGTGCAGGTGGCCATCGCCCCTCCGTTCACCCTGATCGGCGCGCTGCGCGAAGCGG
TGAGCGCCATGGGCGTGGGCGGGCAGGACTGCCACACCGAGGTCAAGGGCGCGCATACCGGCGACGTCTCGGCAGCGATG
CTGGTCGACACCGGGGCCGACTTCGCGATTCTAGGCCACAGCGAGCGCCGCAAGGATCACGGCGAGAACGACGCGCTGGT
CAAGGCCAAGGCCGAAGCGGCGCTGACGGCGGGCCTCGACATCATCGTATGTGTCGGCGAAACGCTGGACCAGCGCGACG
CGGGCAAGGCGGAGGCGGTTGTCTCATCGCAGGTCGACGGTTCGCTTCCTTCACCGGAAACGGCCGCCGAGGCAGTTGCA
GCGGGTAAGGTCGCGGTTGCCTATGAGCCGGTCTGGGCCATCGGCACCGGCCGCGTGGCCGCTGTCGAGGACGTGGTGAC
GATGCACGCCGCAATCCGCGCCCGCCTCGTCGCGCTTTATGGTGAATCCGGCAGCAAGGTCCGCATTCTCTACGGCGGTT
CGGTGAATTCCGGAAACGCCGCGGAACTGCTGGCGGCGGACGGCGTCGGCGGCGCGCTGGTCGGCGGCGCGAGCCTGACG
GCCGAGGCGTTCCTGCCCATCGTCGCGGCGGCGGGTACCGGCGAAGCCTGA

Upstream 100 bases:

>100_bases
CAGAATCGGCGCGGGTTTGTTAGCCGCCGCGACGACTCCGGCCTTCACGGACCGGAAAATACCGATTGCATCAGGGAATT
TACAGGGGAACCGGCCGATC

Downstream 100 bases:

>100_bases
GGCTGCTTCCCGCAAGGGTGGAGCGTCCGACGGGTTGCGCCGAAGGGCGCACGCGCCTACATGGCCAGAAATTCCTGCGC
GGCTTCGCGCCAAGGGTAGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSHRPYIVGNWKMNGSRAMLAEARAIDRAAGRYPDVQVAIAPPFTLIGALREAVSAMGVGGQDCHTEVKGAHTGDVSAAM
LVDTGADFAILGHSERRKDHGENDALVKAKAEAALTAGLDIIVCVGETLDQRDAGKAEAVVSSQVDGSLPSPETAAEAVA
AGKVAVAYEPVWAIGTGRVAAVEDVVTMHAAIRARLVALYGESGSKVRILYGGSVNSGNAAELLAADGVGGALVGGASLT
AEAFLPIVAAAGTGEA

Sequences:

>Translated_256_residues
MSHRPYIVGNWKMNGSRAMLAEARAIDRAAGRYPDVQVAIAPPFTLIGALREAVSAMGVGGQDCHTEVKGAHTGDVSAAM
LVDTGADFAILGHSERRKDHGENDALVKAKAEAALTAGLDIIVCVGETLDQRDAGKAEAVVSSQVDGSLPSPETAAEAVA
AGKVAVAYEPVWAIGTGRVAAVEDVVTMHAAIRARLVALYGESGSKVRILYGGSVNSGNAAELLAADGVGGALVGGASLT
AEAFLPIVAAAGTGEA
>Mature_255_residues
SHRPYIVGNWKMNGSRAMLAEARAIDRAAGRYPDVQVAIAPPFTLIGALREAVSAMGVGGQDCHTEVKGAHTGDVSAAML
VDTGADFAILGHSERRKDHGENDALVKAKAEAALTAGLDIIVCVGETLDQRDAGKAEAVVSSQVDGSLPSPETAAEAVAA
GKVAVAYEPVWAIGTGRVAAVEDVVTMHAAIRARLVALYGESGSKVRILYGGSVNSGNAAELLAADGVGGALVGGASLTA
EAFLPIVAAAGTGEA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=251, Percent_Identity=39.8406374501992, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI226529917, Length=251, Percent_Identity=39.8406374501992, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1790353, Length=254, Percent_Identity=44.8818897637795, Blast_Score=179, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=38.8888888888889, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=39.8406374501992, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI28572004, Length=253, Percent_Identity=43.8735177865613, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572008, Length=253, Percent_Identity=43.8735177865613, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572006, Length=253, Percent_Identity=43.8735177865613, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 25839; Mature: 25708

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRPYIVGNWKMNGSRAMLAEARAIDRAAGRYPDVQVAIAPPFTLIGALREAVSAMGVG
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
GQDCHTEVKGAHTGDVSAAMLVDTGADFAILGHSERRKDHGENDALVKAKAEAALTAGLD
CCHHHHHCCCCCCCCCEEEEEEECCCCEEEECCCHHHHCCCCCCEEEEEHHHHHHHCCCE
IIVCVGETLDQRDAGKAEAVVSSQVDGSLPSPETAAEAVAAGKVAVAYEPVWAIGTGRVA
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCEEEECCCCCH
AVEDVVTMHAAIRARLVALYGESGSKVRILYGGSVNSGNAAELLAADGVGGALVGGASLT
HHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEECCCHHH
AEAFLPIVAAAGTGEA
HHHHHHEEEECCCCCC
>Mature Secondary Structure 
SHRPYIVGNWKMNGSRAMLAEARAIDRAAGRYPDVQVAIAPPFTLIGALREAVSAMGVG
CCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
GQDCHTEVKGAHTGDVSAAMLVDTGADFAILGHSERRKDHGENDALVKAKAEAALTAGLD
CCHHHHHCCCCCCCCCEEEEEEECCCCEEEECCCHHHHCCCCCCEEEEEHHHHHHHCCCE
IIVCVGETLDQRDAGKAEAVVSSQVDGSLPSPETAAEAVAAGKVAVAYEPVWAIGTGRVA
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCEEEECCCCCH
AVEDVVTMHAAIRARLVALYGESGSKVRILYGGSVNSGNAAELLAADGVGGALVGGASLT
HHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEECCCHHH
AEAFLPIVAAAGTGEA
HHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA