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The map label for this gene is bkdA2 [H]

Identifier: 87199992

GI number: 87199992

Start: 2092097

End: 2093152

Strand: Reverse

Name: bkdA2 [H]

Synonym: Saro_1975

Alternate gene names: 87199992

Gene position: 2093152-2092097 (Counterclockwise)

Preceding gene: 87199993

Following gene: 87199991

Centisome position: 58.77

GC content: 61.36

Gene sequence:

>1056_bases
ATGACCTACGAAGAATCTCCGGTCAGCCTCGCCGAGGCCCCGACCCGTCGTCTGAACATGATCGAAGCGATCAATGACGC
TCTCGACATCATGATGGAGCGTGATCCCAACGTCGTGGTCATGGGCGAGGACGTCGGTTATTTCGGCGGCGTGTTCCGTG
CGACGGCGGGACTCCAGAAGAAATACGGCAAGACCCGCGTGTTTGACACGCCGATCAGCGAGTGCGGTATCATCGGCGTG
GCTGTCGGCATGGGCGCTTATGGCCTGCGCCCGGTGCCCGAGATCCAGTTCGCCGACTACATCTATCCAGGCCTCGATCA
GCTCGTTTCGGAGGCTGCGAGGTTGCGTTACCGTTCGGCCGGCGAATTCATTGCGCCGATGACGGTGCGTTCGCCATTTG
GCGGCGGCATCTTCGGCGGGCAGACCCACAGCCAAAGCCCCGAGGCGCTGTTTACCCACGTCGCCGGGCTGAAGACGGTG
GTTCCTAGCACTCCGCACGATGCGAAGGGTCTCTTGATCGCAGCGATCGAGGATAACGATCCGGTGATCTTCTTCGAGCC
CAAGCGCATCTATAACGGGCCTTTCAACGGCTACTACGACAAGCCTGTCGAGCCCTGGAGCAAGCATGCGGACAGCGCCG
TTCCGGAGGGCTATTATTCGATACCGCTAGGAAAGGCCCGCGTCGTGCGCCCGGGGCAGGCGTTCACTGTATTGGCCTAT
GGCACCATGGTCCACGTCGCTGCAGCGGTCTGCGCGGAGAAGGGCGTCGATGCCGAAATCATCGACCTCAGGACACTTGT
CCCGCTGGATATCGAGACGGTGGAAAAGTCGGTGGAAAAGACCGGCAAATGCCTGATCGTCCATGAAGCCACGCGCACTT
CGGGCTTTGGCGCGGAGTTGTCCGCCCTGGTTCAGGAGCGTTGCTTCTACCACCTCGAAGCACCGATAGAGCGCGTGACC
GGCTTCGACACACCCTATCCACACAGCCTCGAATGGGCCTACTTCCCTGGCCCGGTCCGCATCGGCGAGGCCGTCGACCG
ACTGATGAAGGCCTGA

Upstream 100 bases:

>100_bases
TGTTCGAGGACGTCTTCGAGGAACTGCCCTGGCATCTCCGCGAACAGAGCGAGCAGGCAATCCGCGAGCGTCGGATCAAG
TGGCCGGAATGGAAAGAGTC

Downstream 100 bases:

>100_bases
CCCATGGGAACCTACACATTCCGCCTGCCCGATATTGGCGAGGGTATCGCCGAGGCAGAAATCGTCGCCTGGCATGTCAA
GGTCGGCGACACTGTCGAGG

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTYEESPVSLAEAPTRRLNMIEAINDALDIMMERDPNVVVMGEDVGYFGGVFRATAGLQKKYGKTRVFDTPISECGIIGV
AVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSAGEFIAPMTVRSPFGGGIFGGQTHSQSPEALFTHVAGLKTV
VPSTPHDAKGLLIAAIEDNDPVIFFEPKRIYNGPFNGYYDKPVEPWSKHADSAVPEGYYSIPLGKARVVRPGQAFTVLAY
GTMVHVAAAVCAEKGVDAEIIDLRTLVPLDIETVEKSVEKTGKCLIVHEATRTSGFGAELSALVQERCFYHLEAPIERVT
GFDTPYPHSLEWAYFPGPVRIGEAVDRLMKA

Sequences:

>Translated_351_residues
MTYEESPVSLAEAPTRRLNMIEAINDALDIMMERDPNVVVMGEDVGYFGGVFRATAGLQKKYGKTRVFDTPISECGIIGV
AVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSAGEFIAPMTVRSPFGGGIFGGQTHSQSPEALFTHVAGLKTV
VPSTPHDAKGLLIAAIEDNDPVIFFEPKRIYNGPFNGYYDKPVEPWSKHADSAVPEGYYSIPLGKARVVRPGQAFTVLAY
GTMVHVAAAVCAEKGVDAEIIDLRTLVPLDIETVEKSVEKTGKCLIVHEATRTSGFGAELSALVQERCFYHLEAPIERVT
GFDTPYPHSLEWAYFPGPVRIGEAVDRLMKA
>Mature_350_residues
TYEESPVSLAEAPTRRLNMIEAINDALDIMMERDPNVVVMGEDVGYFGGVFRATAGLQKKYGKTRVFDTPISECGIIGVA
VGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSAGEFIAPMTVRSPFGGGIFGGQTHSQSPEALFTHVAGLKTVV
PSTPHDAKGLLIAAIEDNDPVIFFEPKRIYNGPFNGYYDKPVEPWSKHADSAVPEGYYSIPLGKARVVRPGQAFTVLAYG
TMVHVAAAVCAEKGVDAEIIDLRTLVPLDIETVEKSVEKTGKCLIVHEATRTSGFGAELSALVQERCFYHLEAPIERVTG
FDTPYPHSLEWAYFPGPVRIGEAVDRLMKA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=355, Percent_Identity=45.9154929577465, Blast_Score=318, Evalue=4e-87,
Organism=Homo sapiens, GI34101272, Length=355, Percent_Identity=45.9154929577465, Blast_Score=318, Evalue=4e-87,
Organism=Homo sapiens, GI156564403, Length=312, Percent_Identity=39.4230769230769, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI291084858, Length=312, Percent_Identity=38.1410256410256, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17506935, Length=346, Percent_Identity=47.6878612716763, Blast_Score=297, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17538422, Length=350, Percent_Identity=36.8571428571429, Blast_Score=211, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=344, Percent_Identity=35.1744186046512, Blast_Score=196, Evalue=3e-51,
Organism=Drosophila melanogaster, GI160714832, Length=340, Percent_Identity=44.7058823529412, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI160714828, Length=340, Percent_Identity=44.7058823529412, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI21358145, Length=341, Percent_Identity=37.2434017595308, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24650940, Length=341, Percent_Identity=37.2434017595308, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=40.6593406593407, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=40.6593406593407, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI160714826, Length=67, Percent_Identity=47.7611940298507, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 38293; Mature: 38162

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEESPVSLAEAPTRRLNMIEAINDALDIMMERDPNVVVMGEDVGYFGGVFRATAGLQK
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
KYGKTRVFDTPISECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSA
HCCCCEEECCCHHHHCHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCC
GEFIAPMTVRSPFGGGIFGGQTHSQSPEALFTHVAGLKTVVPSTPHDAKGLLIAAIEDND
CCEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
PVIFFEPKRIYNGPFNGYYDKPVEPWSKHADSAVPEGYYSIPLGKARVVRPGQAFTVLAY
CEEEECCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCEEEECCCCEEEEEEH
GTMVHVAAAVCAEKGVDAEIIDLRTLVPLDIETVEKSVEKTGKCLIVHEATRTSGFGAEL
HHHHHHHHHHHHHCCCCCEEEEEHEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
SALVQERCFYHLEAPIERVTGFDTPYPHSLEWAYFPGPVRIGEAVDRLMKA
HHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TYEESPVSLAEAPTRRLNMIEAINDALDIMMERDPNVVVMGEDVGYFGGVFRATAGLQK
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
KYGKTRVFDTPISECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQLVSEAARLRYRSA
HCCCCEEECCCHHHHCHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCC
GEFIAPMTVRSPFGGGIFGGQTHSQSPEALFTHVAGLKTVVPSTPHDAKGLLIAAIEDND
CCEECCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
PVIFFEPKRIYNGPFNGYYDKPVEPWSKHADSAVPEGYYSIPLGKARVVRPGQAFTVLAY
CEEEECCHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEECCCCCEEEECCCCEEEEEEH
GTMVHVAAAVCAEKGVDAEIIDLRTLVPLDIETVEKSVEKTGKCLIVHEATRTSGFGAEL
HHHHHHHHHHHHHCCCCCEEEEEHEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHH
SALVQERCFYHLEAPIERVTGFDTPYPHSLEWAYFPGPVRIGEAVDRLMKA
HHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]