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The map label for this gene is bkdB [H]

Identifier: 87199991

GI number: 87199991

Start: 2090753

End: 2092093

Strand: Reverse

Name: bkdB [H]

Synonym: Saro_1974

Alternate gene names: 87199991

Gene position: 2092093-2090753 (Counterclockwise)

Preceding gene: 87199992

Following gene: 87199990

Centisome position: 58.74

GC content: 65.18

Gene sequence:

>1341_bases
ATGGGAACCTACACATTCCGCCTGCCCGATATTGGCGAGGGTATCGCCGAGGCAGAAATCGTCGCCTGGCATGTCAAGGT
CGGCGACACTGTCGAGGAAGACGGTCGCCTGGCTGACATGATGACCGACAAGGCCACGGTCGAGATGGAAAGCCCGGTCG
CGGGCAAGGTCGTCTCGGTTGCGGGGGAAGTTGGCGATGTCGTGGCGATCGGCTCGGCGCTGGTTGTGATCGAGACCGAG
GGGGAGGACGAGGCACCGGCGCCTGCTGCGGCGCCCGCGCCCAAGGCGGCGATCGTCGAAGAGCGCATCGAGGTCGAAAC
GCCCGAGCCACCGCAACCGCCATCACCGCCCCAGCCGCTGTTCGTTTCGCGCGAAGTCGAGGCACCGCCCGCAGTGCCGG
CTACAGGTTCTGGCGTGGCGCCTGGCCCGCGTGCCTCGACCGCGCCTGACACGATCGGTGGGGCGGGGGCAAAGGTCCTC
GCCAGTCCGGCCGTGCGGCAGCGTGCCCGCGATCTTGGCATAGACCTGTCGGAAGTCCGTCCGTCTGAGGAAGGCCGCAT
TCGCCACGCCGACCTCGATCAGTTCCTCTCCTACAATGCCTCTGGCGGTTACCGTGCAGCCGGTGCCGAGCGCGGCGACG
AAGTGATCAGGGTCATCGGTATGCGGCGACGCATCGCCGAGAACATGGCCGCGTCGAAACGACACATCCCGCACTTCTCC
TACGTCGAGGAATGCGATGTGACCGCGCTTGAAATCATGCGGGAACAACTCAACGCGGGCCGGGGCGACAAGCCCAAGCT
GACGATGTTGCCCCTGCTTATCACCGCGATCTGCCGTGCTCTGCCGCAGTACCCGATGATCAACGCCCGCTATGACGACG
AGGCCGGCGTGGTTACCCGCTATGGTGCGGTGCATCTCGGCATGGCGGCGCAAACGCCTGCGGGCCTTATGGTGCCTGTC
ATCCGCAACGCCCAGACCCTGAATCTCTGGCAACTCGCCCGCGAGATTGTCCGCCTGGCAGAGGCCGCGCGCAGCGGCAG
CGCAAAATCGGACGAGCTTTCCGGTTCGACGTTGACGGTGACGTCCCTTGGCCCACTTGGCGGCGTGGCGACCACGCCGG
TCATCAACCGCCCGGAAGTTGCCATCATCGGGCCCAATCGCATCGTCGAGCGGCCGATGTTCGTGTCCGATGGCATGGGG
GGCGAGCGGATCGAAAAGCGCAAGCTGATGAACATCTCGATCAGTTGCGACCATCGCGTGGTCGATGGCCACGATGCGGC
AAGTTTCATCCAGGCGGTGAAGAAGCTGATCGAAACGCCGGTGCTGCTGCTGGCGGACTGA

Upstream 100 bases:

>100_bases
CGGCTTCGACACACCCTATCCACACAGCCTCGAATGGGCCTACTTCCCTGGCCCGGTCCGCATCGGCGAGGCCGTCGACC
GACTGATGAAGGCCTGACCC

Downstream 100 bases:

>100_bases
TGTGATATGAGCGCGGATCGGAGGTAGCCATGCCGACAACCGATCCGCGCATCGACGAGCATATCGCGAAGGCAGGTGCG
TTTGCCCGGCCCGTGCTGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MGTYTFRLPDIGEGIAEAEIVAWHVKVGDTVEEDGRLADMMTDKATVEMESPVAGKVVSVAGEVGDVVAIGSALVVIETE
GEDEAPAPAAAPAPKAAIVEERIEVETPEPPQPPSPPQPLFVSREVEAPPAVPATGSGVAPGPRASTAPDTIGGAGAKVL
ASPAVRQRARDLGIDLSEVRPSEEGRIRHADLDQFLSYNASGGYRAAGAERGDEVIRVIGMRRRIAENMAASKRHIPHFS
YVEECDVTALEIMREQLNAGRGDKPKLTMLPLLITAICRALPQYPMINARYDDEAGVVTRYGAVHLGMAAQTPAGLMVPV
IRNAQTLNLWQLAREIVRLAEAARSGSAKSDELSGSTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVSDGMG
GERIEKRKLMNISISCDHRVVDGHDAASFIQAVKKLIETPVLLLAD

Sequences:

>Translated_446_residues
MGTYTFRLPDIGEGIAEAEIVAWHVKVGDTVEEDGRLADMMTDKATVEMESPVAGKVVSVAGEVGDVVAIGSALVVIETE
GEDEAPAPAAAPAPKAAIVEERIEVETPEPPQPPSPPQPLFVSREVEAPPAVPATGSGVAPGPRASTAPDTIGGAGAKVL
ASPAVRQRARDLGIDLSEVRPSEEGRIRHADLDQFLSYNASGGYRAAGAERGDEVIRVIGMRRRIAENMAASKRHIPHFS
YVEECDVTALEIMREQLNAGRGDKPKLTMLPLLITAICRALPQYPMINARYDDEAGVVTRYGAVHLGMAAQTPAGLMVPV
IRNAQTLNLWQLAREIVRLAEAARSGSAKSDELSGSTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVSDGMG
GERIEKRKLMNISISCDHRVVDGHDAASFIQAVKKLIETPVLLLAD
>Mature_445_residues
GTYTFRLPDIGEGIAEAEIVAWHVKVGDTVEEDGRLADMMTDKATVEMESPVAGKVVSVAGEVGDVVAIGSALVVIETEG
EDEAPAPAAAPAPKAAIVEERIEVETPEPPQPPSPPQPLFVSREVEAPPAVPATGSGVAPGPRASTAPDTIGGAGAKVLA
SPAVRQRARDLGIDLSEVRPSEEGRIRHADLDQFLSYNASGGYRAAGAERGDEVIRVIGMRRRIAENMAASKRHIPHFSY
VEECDVTALEIMREQLNAGRGDKPKLTMLPLLITAICRALPQYPMINARYDDEAGVVTRYGAVHLGMAAQTPAGLMVPVI
RNAQTLNLWQLAREIVRLAEAARSGSAKSDELSGSTLTVTSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVSDGMGG
ERIEKRKLMNISISCDHRVVDGHDAASFIQAVKKLIETPVLLLAD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=470, Percent_Identity=28.7234042553192, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI31711992, Length=459, Percent_Identity=27.0152505446623, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=31.140350877193, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI203098816, Length=223, Percent_Identity=28.2511210762332, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI203098753, Length=224, Percent_Identity=28.125, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=27.5449101796407, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786305, Length=303, Percent_Identity=29.3729372937294, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786946, Length=458, Percent_Identity=25.764192139738, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=464, Percent_Identity=29.7413793103448, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=460, Percent_Identity=30.6521739130435, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=446, Percent_Identity=27.1300448430493, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=239, Percent_Identity=26.7782426778243, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320352, Length=442, Percent_Identity=26.9230769230769, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=25.0539956803456, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=29.7959183673469, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=27.6315789473684, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47318; Mature: 47186

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTYTFRLPDIGEGIAEAEIVAWHVKVGDTVEEDGRLADMMTDKATVEMESPVAGKVVSV
CCEEEEECCCCCCCCCCCEEEEEEEEECCCHHCCCCCHHHHCCCCEEEECCCCCCHHHHH
AGEVGDVVAIGSALVVIETEGEDEAPAPAAAPAPKAAIVEERIEVETPEPPQPPSPPQPL
HCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCE
FVSREVEAPPAVPATGSGVAPGPRASTAPDTIGGAGAKVLASPAVRQRARDLGIDLSEVR
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHCC
PSEEGRIRHADLDQFLSYNASGGYRAAGAERGDEVIRVIGMRRRIAENMAASKRHIPHFS
CCCCCCEECCCHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
YVEECDVTALEIMREQLNAGRGDKPKLTMLPLLITAICRALPQYPMINARYDDEAGVVTR
HHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
YGAVHLGMAAQTPAGLMVPVIRNAQTLNLWQLAREIVRLAEAARSGSAKSDELSGSTLTV
CCCEEECCCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVSDGMGGERIEKRKLMNISISCDHRV
EECCCCCCCCCCCCCCCCCEEEECCHHHHCCCCEEECCCCHHHHHHHHEEEEEEECCCEE
VDGHDAASFIQAVKKLIETPVLLLAD
ECCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
GTYTFRLPDIGEGIAEAEIVAWHVKVGDTVEEDGRLADMMTDKATVEMESPVAGKVVSV
CEEEEECCCCCCCCCCCEEEEEEEEECCCHHCCCCCHHHHCCCCEEEECCCCCCHHHHH
AGEVGDVVAIGSALVVIETEGEDEAPAPAAAPAPKAAIVEERIEVETPEPPQPPSPPQPL
HCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCE
FVSREVEAPPAVPATGSGVAPGPRASTAPDTIGGAGAKVLASPAVRQRARDLGIDLSEVR
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCHHHCC
PSEEGRIRHADLDQFLSYNASGGYRAAGAERGDEVIRVIGMRRRIAENMAASKRHIPHFS
CCCCCCEECCCHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
YVEECDVTALEIMREQLNAGRGDKPKLTMLPLLITAICRALPQYPMINARYDDEAGVVTR
HHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
YGAVHLGMAAQTPAGLMVPVIRNAQTLNLWQLAREIVRLAEAARSGSAKSDELSGSTLTV
CCCEEECCCCCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
TSLGPLGGVATTPVINRPEVAIIGPNRIVERPMFVSDGMGGERIEKRKLMNISISCDHRV
EECCCCCCCCCCCCCCCCCEEEECCHHHHCCCCEEECCCCHHHHHHHHEEEEEEECCCEE
VDGHDAASFIQAVKKLIETPVLLLAD
ECCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]