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The map label for this gene is rafA [H]

Identifier: 87199895

GI number: 87199895

Start: 1979797

End: 1981896

Strand: Direct

Name: rafA [H]

Synonym: Saro_1878

Alternate gene names: 87199895

Gene position: 1979797-1981896 (Clockwise)

Preceding gene: 87199894

Following gene: 87199896

Centisome position: 55.59

GC content: 68.48

Gene sequence:

>2100_bases
ATGGGCGAGGTATTCGCCTTGCACTCCGGGGACGGCAGTCTCGTCTGGGAAGCGGACGACGGCAGCGCGCCCGCGTGGCG
GCACTTCGGCCCCCGGCTGACAGCCGACGGGATCCGGCCGATCCGGGACCAGCGCGCGCCTGCGTCATACTCGCTGGACG
ACGACGTACCGTTCGCCGTCGCACCGGCGGCCGGGCTGGGCTGGTTCGGACCTTCGGCCATGTGCTTGCGGCGCGGCTCA
GTCGCACTCGTGCCGGCAATGGCGGGGGCGTCCGTCGCCGGGGATGCCGGCTCCGTGCGCATCATCACGCGCGATCCGGT
TGCGGGCGTGGAGCTTGAACAGGTGTTCGAAGCAGTTGGCGGTGCTTTCGTCTGCCGCAGCGTCGTGCGCAACATCGGCA
GTGAAACGTTCGAGGTCGACTGGCTGTCGAGCGCGCTCCTCCCCATCGCGGGTTCCGCGCGCGAGATCGTGTCGTGGCGT
GGACGCCACAACGCCGAACTGGTCGAATGCCGCGAGCCGATGCCGCAGCATTCGTGGGTGCGCGAAGGTCGGCGCGGGAT
TTCCGGGCACGGCGGCCCTCCGGGCCTGTTCGTGCTCGACGAAGGCGCTACGTATCACGCGGGCACTGTCCGCGCGCTGC
AGCTTGCGTGGTCTGGCGATGCGCGCATCGAGGTCGAGCGCGACGACGAGGGTTTCTGGACGTTGAACGCGGGCGCGGTG
CTTCAACCTGGCGAGGCCAGCCTTGCGCCCGGCGAGAGCTGGCAGTCGCCCGACGCCATCGTGACGGTATCAACCTCCGG
CCGGAACGGCGCGGCGCAGGCGTTTCACGATGCAGTCCGCGCGCGCATCCGCTGGCCGGACGGGGCCATGCGCCCACGGC
CCGTGCACCTCAACTCGTGGGAAGCCTGCTATTTCGACCACGACGAGGAGCGCATCGTTGCCCTTGCGGAAGCGGCGGCA
TCCGTGGGCGTGGAGCGGTTCATTCTCGACGATGGCTGGTTCCGGGGCCGCAACGACGACACGGCGGGACTGGGCGACTG
GACAACCGATCCCGTCAAGTATCCGCACGGACTGCGCACCCTGGCAGACCGCGTCAATGCGCTGGGCATGGAGTTCGGGC
TCTGGGTCGAACCGGAAATGATCAATCCCGATAGCGACCTCTATCGGGCGTATCCGGACTGGGCGCTTGCGCTTCCGGGG
CGGAAACGACCGACTGCCCGGAACCAGCTCGTGCTTGACATGCGGCGACGGGACGTTCGCGACCACCTCTTCGGCTGCAT
CGACGCGCTGCTGCGCGAATTGCCGATCACCTACCTCAAGTGGGACCACAACCGCGACCTCGCGCCTGCTGGCGGGGCGG
CGCAGATGAGGGGGGCGTATGAACTGTTCGCGCGGGTCCGCGCAGCGCATCCCGCCGTGGAGATCGAGGCCTGTGCTGGC
GGCGGCGGGCGCAACGATGCAGGTATGGCCGACTATTGCCATCGCTACTGGACCAGCGACAATATCGACGCGGCCAGTCG
CATCGGTATCCAGCGCGGGTTCCTGTCGTTCCTCCCTCCCGAGGTGATGGGATCGCACATCGCCGCAAGCCCCGCCCATG
CCACGGGGCGCAGGCATTCCCTGGGCTTCCGGGCTGCCATGGCGATGGCCGGTCATCTCGGCGTGGAAATGGACCCGCGC
ACGCTGGGCGATGCAGAGCGCGCCGAACTGGCCGACTGGATCGCCTTTCACAAGCAATGGCGCGGATTGCTGCACCAGGG
CACTGTCTGGCTGGGCGAGGGTGCGGATGCAGCGTTCTGGCAGGCGCAGGGGAATGCAGCGGAACTGCTGCTCTTCGTGA
TCCGCGCGGACCCGCCGCTGGACCGTCGCCCGCAGCCTTTGCCACTGCCTTTCGCGGGCCAGGATGGAACATGGGACATC
CGGCTTCTTCGTATCGCCGGGGGCGAGGGCGGGCATGCCGCGCATTCCGCTGCGCTGTTCGAGGCAATGAAGGCCGCCCC
TCAGGCGTTCCCGGCTGACTGGCTCTCGGCCAACGGCCTGCCGCTCCCGCCCTGCAAGGCGGAGACGGTCACCATCTTCC
ATCTGCGCAAACGCGCCTGA

Upstream 100 bases:

>100_bases
TGATGTTTTGCGGCGCGTCGCTGGTGCTCGCGGGCTTCTCGGTGCTCTGGGTGCGCGAAGGACGAGCCGGCGCGATGCCG
CAATCGGCGGTGCCGGCCTG

Downstream 100 bases:

>100_bases
TCTGTCTTAAGCCAAATCTGTTTCAAGAAAGTCGGATATGTCCGTGGTCAATCCATCAGAACAATCGATTGCCTCGTTTG
GCGCGACGCCGTGGCGTCCC

Product: glycoside hydrolase, clan GH-D

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MGEVFALHSGDGSLVWEADDGSAPAWRHFGPRLTADGIRPIRDQRAPASYSLDDDVPFAVAPAAGLGWFGPSAMCLRRGS
VALVPAMAGASVAGDAGSVRIITRDPVAGVELEQVFEAVGGAFVCRSVVRNIGSETFEVDWLSSALLPIAGSAREIVSWR
GRHNAELVECREPMPQHSWVREGRRGISGHGGPPGLFVLDEGATYHAGTVRALQLAWSGDARIEVERDDEGFWTLNAGAV
LQPGEASLAPGESWQSPDAIVTVSTSGRNGAAQAFHDAVRARIRWPDGAMRPRPVHLNSWEACYFDHDEERIVALAEAAA
SVGVERFILDDGWFRGRNDDTAGLGDWTTDPVKYPHGLRTLADRVNALGMEFGLWVEPEMINPDSDLYRAYPDWALALPG
RKRPTARNQLVLDMRRRDVRDHLFGCIDALLRELPITYLKWDHNRDLAPAGGAAQMRGAYELFARVRAAHPAVEIEACAG
GGGRNDAGMADYCHRYWTSDNIDAASRIGIQRGFLSFLPPEVMGSHIAASPAHATGRRHSLGFRAAMAMAGHLGVEMDPR
TLGDAERAELADWIAFHKQWRGLLHQGTVWLGEGADAAFWQAQGNAAELLLFVIRADPPLDRRPQPLPLPFAGQDGTWDI
RLLRIAGGEGGHAAHSAALFEAMKAAPQAFPADWLSANGLPLPPCKAETVTIFHLRKRA

Sequences:

>Translated_699_residues
MGEVFALHSGDGSLVWEADDGSAPAWRHFGPRLTADGIRPIRDQRAPASYSLDDDVPFAVAPAAGLGWFGPSAMCLRRGS
VALVPAMAGASVAGDAGSVRIITRDPVAGVELEQVFEAVGGAFVCRSVVRNIGSETFEVDWLSSALLPIAGSAREIVSWR
GRHNAELVECREPMPQHSWVREGRRGISGHGGPPGLFVLDEGATYHAGTVRALQLAWSGDARIEVERDDEGFWTLNAGAV
LQPGEASLAPGESWQSPDAIVTVSTSGRNGAAQAFHDAVRARIRWPDGAMRPRPVHLNSWEACYFDHDEERIVALAEAAA
SVGVERFILDDGWFRGRNDDTAGLGDWTTDPVKYPHGLRTLADRVNALGMEFGLWVEPEMINPDSDLYRAYPDWALALPG
RKRPTARNQLVLDMRRRDVRDHLFGCIDALLRELPITYLKWDHNRDLAPAGGAAQMRGAYELFARVRAAHPAVEIEACAG
GGGRNDAGMADYCHRYWTSDNIDAASRIGIQRGFLSFLPPEVMGSHIAASPAHATGRRHSLGFRAAMAMAGHLGVEMDPR
TLGDAERAELADWIAFHKQWRGLLHQGTVWLGEGADAAFWQAQGNAAELLLFVIRADPPLDRRPQPLPLPFAGQDGTWDI
RLLRIAGGEGGHAAHSAALFEAMKAAPQAFPADWLSANGLPLPPCKAETVTIFHLRKRA
>Mature_698_residues
GEVFALHSGDGSLVWEADDGSAPAWRHFGPRLTADGIRPIRDQRAPASYSLDDDVPFAVAPAAGLGWFGPSAMCLRRGSV
ALVPAMAGASVAGDAGSVRIITRDPVAGVELEQVFEAVGGAFVCRSVVRNIGSETFEVDWLSSALLPIAGSAREIVSWRG
RHNAELVECREPMPQHSWVREGRRGISGHGGPPGLFVLDEGATYHAGTVRALQLAWSGDARIEVERDDEGFWTLNAGAVL
QPGEASLAPGESWQSPDAIVTVSTSGRNGAAQAFHDAVRARIRWPDGAMRPRPVHLNSWEACYFDHDEERIVALAEAAAS
VGVERFILDDGWFRGRNDDTAGLGDWTTDPVKYPHGLRTLADRVNALGMEFGLWVEPEMINPDSDLYRAYPDWALALPGR
KRPTARNQLVLDMRRRDVRDHLFGCIDALLRELPITYLKWDHNRDLAPAGGAAQMRGAYELFARVRAAHPAVEIEACAGG
GGRNDAGMADYCHRYWTSDNIDAASRIGIQRGFLSFLPPEVMGSHIAASPAHATGRRHSLGFRAAMAMAGHLGVEMDPRT
LGDAERAELADWIAFHKQWRGLLHQGTVWLGEGADAAFWQAQGNAAELLLFVIRADPPLDRRPQPLPLPFAGQDGTWDIR
LLRIAGGEGGHAAHSAALFEAMKAAPQAFPADWLSANGLPLPPCKAETVTIFHLRKRA

Specific function: Unknown

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 75843; Mature: 75712

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00512 ALPHA_GALACTOSIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEVFALHSGDGSLVWEADDGSAPAWRHFGPRLTADGIRPIRDQRAPASYSLDDDVPFAV
CCCEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEE
APAAGLGWFGPSAMCLRRGSVALVPAMAGASVAGDAGSVRIITRDPVAGVELEQVFEAVG
ECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHC
GAFVCRSVVRNIGSETFEVDWLSSALLPIAGSAREIVSWRGRHNAELVECREPMPQHSWV
HHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCCCCHHHH
REGRRGISGHGGPPGLFVLDEGATYHAGTVRALQLAWSGDARIEVERDDEGFWTLNAGAV
HHHHCCCCCCCCCCEEEEECCCCEEECCCEEEEEEEECCCCEEEEEECCCCEEEECCCCE
LQPGEASLAPGESWQSPDAIVTVSTSGRNGAAQAFHDAVRARIRWPDGAMRPRPVHLNSW
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEECCCC
EACYFDHDEERIVALAEAAASVGVERFILDDGWFRGRNDDTAGLGDWTTDPVKYPHGLRT
CEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LADRVNALGMEFGLWVEPEMINPDSDLYRAYPDWALALPGRKRPTARNQLVLDMRRRDVR
HHHHHHHCCCCCCCEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
DHLFGCIDALLRELPITYLKWDHNRDLAPAGGAAQMRGAYELFARVRAAHPAVEIEACAG
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGGRNDAGMADYCHRYWTSDNIDAASRIGIQRGFLSFLPPEVMGSHIAASPAHATGRRHS
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCC
LGFRAAMAMAGHLGVEMDPRTLGDAERAELADWIAFHKQWRGLLHQGTVWLGEGADAAFW
CHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEE
QAQGNAAELLLFVIRADPPLDRRPQPLPLPFAGQDGTWDIRLLRIAGGEGGHAAHSAALF
ECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHH
EAMKAAPQAFPADWLSANGLPLPPCKAETVTIFHLRKRA
HHHHHCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
GEVFALHSGDGSLVWEADDGSAPAWRHFGPRLTADGIRPIRDQRAPASYSLDDDVPFAV
CCEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEE
APAAGLGWFGPSAMCLRRGSVALVPAMAGASVAGDAGSVRIITRDPVAGVELEQVFEAVG
ECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHC
GAFVCRSVVRNIGSETFEVDWLSSALLPIAGSAREIVSWRGRHNAELVECREPMPQHSWV
HHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCCCCHHHH
REGRRGISGHGGPPGLFVLDEGATYHAGTVRALQLAWSGDARIEVERDDEGFWTLNAGAV
HHHHCCCCCCCCCCEEEEECCCCEEECCCEEEEEEEECCCCEEEEEECCCCEEEECCCCE
LQPGEASLAPGESWQSPDAIVTVSTSGRNGAAQAFHDAVRARIRWPDGAMRPRPVHLNSW
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEECCCC
EACYFDHDEERIVALAEAAASVGVERFILDDGWFRGRNDDTAGLGDWTTDPVKYPHGLRT
CEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
LADRVNALGMEFGLWVEPEMINPDSDLYRAYPDWALALPGRKRPTARNQLVLDMRRRDVR
HHHHHHHCCCCCCCEECCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
DHLFGCIDALLRELPITYLKWDHNRDLAPAGGAAQMRGAYELFARVRAAHPAVEIEACAG
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
GGGRNDAGMADYCHRYWTSDNIDAASRIGIQRGFLSFLPPEVMGSHIAASPAHATGRRHS
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCC
LGFRAAMAMAGHLGVEMDPRTLGDAERAELADWIAFHKQWRGLLHQGTVWLGEGADAAFW
CHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEE
QAQGNAAELLLFVIRADPPLDRRPQPLPLPFAGQDGTWDIRLLRIAGGEGGHAAHSAALF
ECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHH
EAMKAAPQAFPADWLSANGLPLPPCKAETVTIFHLRKRA
HHHHHCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2556373; 2180920; 786627 [H]