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The map label for this gene is trxB [C]

Identifier: 87199630

GI number: 87199630

Start: 1682054

End: 1682995

Strand: Direct

Name: trxB [C]

Synonym: Saro_1613

Alternate gene names: 87199630

Gene position: 1682054-1682995 (Clockwise)

Preceding gene: 87199628

Following gene: 87199631

Centisome position: 47.23

GC content: 63.06

Gene sequence:

>942_bases
ATGCAACCAAACCTCGACTGCCTCATCATTGGCGGCGGCCCTGCTGGGCTGACCGCCGCAATATATCTCGCGCGATTTCA
TTTGCGCATTGCCGTGGTCGATGCCGGAGGAGGCCGCGCCGCGATGATCCCCCGGACGCGCAACCATTCCGGCTTTCCCG
GCGGAATCTCGGGCAGGGATCTGCTGGCGCGCATGGAAGAACAGGCGGCGGAGTTCGGCGTGGTGGTCGAGCAGACGACC
GTCGAAGCGATTGACAGGGTCGATGGCGCCTTTCGTGCCGTGGCGCAGGGTCGGGAATGGATCGCGCAAACGGTGCTCGT
TGCAACGGGCGTGATCAACAACCGCCCCCCGATCTTGCCAGAGGTCCATGATCGGGCCCTTCAGCAGGGGCTGCTTCGCT
ATTGTCCGATCTGCGACGGTTACGAAGTGACCGACCGCGACGTCGGGGTAATAGGAACCGGCGATCACGGCCGCGGGGAA
GCCCTGTTCCTGCGCGCTTACACGGCCAACATCACGCTGATTGCGCCCGATGGTCCCCATGATCTGGACGCAGAGGCCCG
CAGGGAACTGGAAGATGTTGGCGTGCGGCTCATCGATGGCCCTTGCCATGCGCTCCGGATCGACGGTGGAAATATCTTTG
CTTCGACCCCAGTGGCAGAGCTTGGTTTCCATACGATCTACCCCGCTTTGGGTTCAAAAATCTGTTCCGATTTGGCACGA
TCGCTTGGGGCGGAGGCGAGCGAAGACGGTTGCCTGGTTATCGACCGGCACCAGCGGACGAGCATCGAGGGGCTCTACGC
AGCGGGTGACGTGACCAAGGGACTGGACCAGATAAGCCACGCGATGGGCGAAGCGGCAGTTGCTGCAACGGCGATCCGCA
ACGATCTGCGCGCACGCCGTCCGTTATTGCGCAGGCGTGCCGCGCCCCTGACAACGGACTGA

Upstream 100 bases:

>100_bases
TCGTCGCACGAGAGGCGCAGCCCGTCGCAGGCCAAGCGCGTGGCGTGAGACGTCGCGCAGCACGCAGGAACCGCCTGCGG
GGCAGGTGACTTGGCATGGG

Downstream 100 bases:

>100_bases
CAATGTTACTAACAAACACTGGATTTTCAATAATCTGGTTAATGCGTTCGGTATGCAATTATTAAGGCTTTAACCAGTTT
TTGGCTCTCCTGTCGGTTTA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MQPNLDCLIIGGGPAGLTAAIYLARFHLRIAVVDAGGGRAAMIPRTRNHSGFPGGISGRDLLARMEEQAAEFGVVVEQTT
VEAIDRVDGAFRAVAQGREWIAQTVLVATGVINNRPPILPEVHDRALQQGLLRYCPICDGYEVTDRDVGVIGTGDHGRGE
ALFLRAYTANITLIAPDGPHDLDAEARRELEDVGVRLIDGPCHALRIDGGNIFASTPVAELGFHTIYPALGSKICSDLAR
SLGAEASEDGCLVIDRHQRTSIEGLYAAGDVTKGLDQISHAMGEAAVAATAIRNDLRARRPLLRRRAAPLTTD

Sequences:

>Translated_313_residues
MQPNLDCLIIGGGPAGLTAAIYLARFHLRIAVVDAGGGRAAMIPRTRNHSGFPGGISGRDLLARMEEQAAEFGVVVEQTT
VEAIDRVDGAFRAVAQGREWIAQTVLVATGVINNRPPILPEVHDRALQQGLLRYCPICDGYEVTDRDVGVIGTGDHGRGE
ALFLRAYTANITLIAPDGPHDLDAEARRELEDVGVRLIDGPCHALRIDGGNIFASTPVAELGFHTIYPALGSKICSDLAR
SLGAEASEDGCLVIDRHQRTSIEGLYAAGDVTKGLDQISHAMGEAAVAATAIRNDLRARRPLLRRRAAPLTTD
>Mature_313_residues
MQPNLDCLIIGGGPAGLTAAIYLARFHLRIAVVDAGGGRAAMIPRTRNHSGFPGGISGRDLLARMEEQAAEFGVVVEQTT
VEAIDRVDGAFRAVAQGREWIAQTVLVATGVINNRPPILPEVHDRALQQGLLRYCPICDGYEVTDRDVGVIGTGDHGRGE
ALFLRAYTANITLIAPDGPHDLDAEARRELEDVGVRLIDGPCHALRIDGGNIFASTPVAELGFHTIYPALGSKICSDLAR
SLGAEASEDGCLVIDRHQRTSIEGLYAAGDVTKGLDQISHAMGEAAVAATAIRNDLRARRPLLRRRAAPLTTD

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=169, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321898, Length=285, Percent_Identity=27.3684210526316, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320560, Length=291, Percent_Identity=28.5223367697594, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 33348; Mature: 33348

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPNLDCLIIGGGPAGLTAAIYLARFHLRIAVVDAGGGRAAMIPRTRNHSGFPGGISGRD
CCCCCCEEEECCCCHHHHHHHHHHHEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHH
LLARMEEQAAEFGVVVEQTTVEAIDRVDGAFRAVAQGREWIAQTVLVATGVINNRPPILP
HHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
EVHDRALQQGLLRYCPICDGYEVTDRDVGVIGTGDHGRGEALFLRAYTANITLIAPDGPH
HHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCEEEEEEEECEEEEECCCCCC
DLDAEARRELEDVGVRLIDGPCHALRIDGGNIFASTPVAELGFHTIYPALGSKICSDLAR
CCCHHHHHHHHHCCEEEEECCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SLGAEASEDGCLVIDRHQRTSIEGLYAAGDVTKGLDQISHAMGEAAVAATAIRNDLRARR
HHCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLRRRAAPLTTD
HHHHHCCCCCCCC
>Mature Secondary Structure
MQPNLDCLIIGGGPAGLTAAIYLARFHLRIAVVDAGGGRAAMIPRTRNHSGFPGGISGRD
CCCCCCEEEECCCCHHHHHHHHHHHEEEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHH
LLARMEEQAAEFGVVVEQTTVEAIDRVDGAFRAVAQGREWIAQTVLVATGVINNRPPILP
HHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
EVHDRALQQGLLRYCPICDGYEVTDRDVGVIGTGDHGRGEALFLRAYTANITLIAPDGPH
HHHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCCCCCCEEEEEEEECEEEEECCCCCC
DLDAEARRELEDVGVRLIDGPCHALRIDGGNIFASTPVAELGFHTIYPALGSKICSDLAR
CCCHHHHHHHHHCCEEEEECCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
SLGAEASEDGCLVIDRHQRTSIEGLYAAGDVTKGLDQISHAMGEAAVAATAIRNDLRARR
HHCCCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLLRRRAAPLTTD
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.125 {thioredoxin} 0.008 {NADPH}} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]