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The map label for this gene is kstD [H]

Identifier: 87199597

GI number: 87199597

Start: 1636020

End: 1637672

Strand: Direct

Name: kstD [H]

Synonym: Saro_1580

Alternate gene names: 87199597

Gene position: 1636020-1637672 (Clockwise)

Preceding gene: 87199595

Following gene: 87199604

Centisome position: 45.94

GC content: 65.64

Gene sequence:

>1653_bases
ATGAGCGAGCAGCAGAACTGGGATATCGAAGCGGACGTCGTGGTCCTCGGCTCGGGCGGCGCCGCAATGACGGCCGCCAT
CGCGGCGCATGATTTCGGCGCATCCGAGGTGGTGATCCTCGAGAGGTCGGGCATGGTCGGCGGCACGACCGCGATGTCCG
GCGGGATGCTGTGGATTCCCAACAACCATCACATGCACGAGGAAGGCATCGAGGATTCCGACGAGGAGGTCGTGACCTAT
CTCGATTCCCTGGCCCCGGGCGCGCTCGATCCCGAAACGCTCTGGTCGTTCATGCAGAACGGCCCGGAAATGCTGCGCTA
CCTTGCGGAACGCACGCCGGTCCGCCTGCGTGCGTTCAAGGACTTCCCCGATTACCAACCCTATTCCCCCGGCGCGAAGC
CGGACGGCGGGCGCTCGCTCGACAACGAGGCGTTCTCGTTCGAGCGGCTGGGCAAGTGGGCCGGAAGGGTCAATCCCAGC
AAGATGGCCTATCCCCTGCGCGGCAGCCTGATGGAGGCGATCAGCGGTTCGCTGGACGAAGCGACGCTGGCGGAACGCGA
GGCTGGCGACTATCGCGGACTGGGGCAGGCACTCGCCGGCGCGCTGTTCATGGCCGTGCTGGATCGCAACATCCCCGTCG
AATTCGAGAAGCGCGCCCGCAAGCTCGTCAAGGACGGCGACAGGGTGATCGGCGTCATCGCCGATGACGCCAACGGCAAG
CCTTTCCGCGTCCGTGCACGACGCGGTGTGGTGATCGCCACCGGTGGCTTCGAGTGGAACGAGACTCTCGTGAAGGCATT
CGTGCGCGGGCCGCTCACCGGCCCGGTCAGCGTGCCCGAGAACGAAGGTGACGGCCTGCTCATGGCGATCGAGGCCGGTG
CGCAACTCGGCAACATGCAGAATGCGTTCTGGATGCAGAGCGTGCTAGAGTTCAAGCCGCAGCACCGCAATGCCAAGCCG
AACTACCTGCTCGGCTCGGACGAGCGCGCCCGGCCCGGCGCGATCCTCGTGAACCGCGCCGGCAAGCGCTTCGTCAACGA
GGCGGCCAACTACAACGCACTCGGCAAGTCGCTCCACGCCTTCGACGCGGGCACGCACACATATGCGAACCTGCCGTACT
GGTTGATCATCGACCAGCGTTACAAGGACAAGTACCACACCTTCAACTCGCCTCCGGGCGGGCCGACGCCTTCCTTCATG
ATGAAGGCCGATACGCTCGAGGAACTGGCCGAGGTCGCCGGTATCGATCCGCAAGGCCTGGTCGCTACAGTTGCGCGGTT
CAACGACATGGTGCGCAAGGGCCACGACGACGACTTCAATCGCGGCGACAACTCCTACGACAACTTCTACATGTGGGGCG
ACACCGACTTCGACCCGCCCTACCGCACGCTCGGCGTGATCGACCAGGGGCCGTTCTATGCGGTGAAGATGGAAGCAGGC
GCTCTCGGCACGGCGGGCGGACCCAGGACCAATGCCGACGCGCAGGTGGTCGACTGGAACGGCAATCCGATCCCGGGGCT
CTACGCGGCAGGCAATGCGATGGCGGCGGTGCTGGGCGAGATCTACGGCGGCGCGGGCGGCACGCTGGGCCCGGGCCTGA
CCTTCGGTTATGTCGCGGGCCGCCATCTCGGCAGCCACATCTCGAACCACTGA

Upstream 100 bases:

>100_bases
GTAGTGGCCTGATGTCACCATGCAATTCTAGACTGCAATCAAATCAACAGGCTGGCGGCCATCCGCCTTGCCGTAAAACA
ATATGACGGGACGACGGGTG

Downstream 100 bases:

>100_bases
AGAAGTGCGCAGTCGGCCTGTGCAAAGCGGGTCGACTGCGCACGATATGGCCGCTTCGGGGGCGGTAACCGCATTGCGGC
AACCCTGCGGCGCGTCCGGT

Product: fumarate reductase/succinate dehydrogenase flavoprotein-like protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSEQQNWDIEADVVVLGSGGAAMTAAIAAHDFGASEVVILERSGMVGGTTAMSGGMLWIPNNHHMHEEGIEDSDEEVVTY
LDSLAPGALDPETLWSFMQNGPEMLRYLAERTPVRLRAFKDFPDYQPYSPGAKPDGGRSLDNEAFSFERLGKWAGRVNPS
KMAYPLRGSLMEAISGSLDEATLAEREAGDYRGLGQALAGALFMAVLDRNIPVEFEKRARKLVKDGDRVIGVIADDANGK
PFRVRARRGVVIATGGFEWNETLVKAFVRGPLTGPVSVPENEGDGLLMAIEAGAQLGNMQNAFWMQSVLEFKPQHRNAKP
NYLLGSDERARPGAILVNRAGKRFVNEAANYNALGKSLHAFDAGTHTYANLPYWLIIDQRYKDKYHTFNSPPGGPTPSFM
MKADTLEELAEVAGIDPQGLVATVARFNDMVRKGHDDDFNRGDNSYDNFYMWGDTDFDPPYRTLGVIDQGPFYAVKMEAG
ALGTAGGPRTNADAQVVDWNGNPIPGLYAAGNAMAAVLGEIYGGAGGTLGPGLTFGYVAGRHLGSHISNH

Sequences:

>Translated_550_residues
MSEQQNWDIEADVVVLGSGGAAMTAAIAAHDFGASEVVILERSGMVGGTTAMSGGMLWIPNNHHMHEEGIEDSDEEVVTY
LDSLAPGALDPETLWSFMQNGPEMLRYLAERTPVRLRAFKDFPDYQPYSPGAKPDGGRSLDNEAFSFERLGKWAGRVNPS
KMAYPLRGSLMEAISGSLDEATLAEREAGDYRGLGQALAGALFMAVLDRNIPVEFEKRARKLVKDGDRVIGVIADDANGK
PFRVRARRGVVIATGGFEWNETLVKAFVRGPLTGPVSVPENEGDGLLMAIEAGAQLGNMQNAFWMQSVLEFKPQHRNAKP
NYLLGSDERARPGAILVNRAGKRFVNEAANYNALGKSLHAFDAGTHTYANLPYWLIIDQRYKDKYHTFNSPPGGPTPSFM
MKADTLEELAEVAGIDPQGLVATVARFNDMVRKGHDDDFNRGDNSYDNFYMWGDTDFDPPYRTLGVIDQGPFYAVKMEAG
ALGTAGGPRTNADAQVVDWNGNPIPGLYAAGNAMAAVLGEIYGGAGGTLGPGLTFGYVAGRHLGSHISNH
>Mature_549_residues
SEQQNWDIEADVVVLGSGGAAMTAAIAAHDFGASEVVILERSGMVGGTTAMSGGMLWIPNNHHMHEEGIEDSDEEVVTYL
DSLAPGALDPETLWSFMQNGPEMLRYLAERTPVRLRAFKDFPDYQPYSPGAKPDGGRSLDNEAFSFERLGKWAGRVNPSK
MAYPLRGSLMEAISGSLDEATLAEREAGDYRGLGQALAGALFMAVLDRNIPVEFEKRARKLVKDGDRVIGVIADDANGKP
FRVRARRGVVIATGGFEWNETLVKAFVRGPLTGPVSVPENEGDGLLMAIEAGAQLGNMQNAFWMQSVLEFKPQHRNAKPN
YLLGSDERARPGAILVNRAGKRFVNEAANYNALGKSLHAFDAGTHTYANLPYWLIIDQRYKDKYHTFNSPPGGPTPSFMM
KADTLEELAEVAGIDPQGLVATVARFNDMVRKGHDDDFNRGDNSYDNFYMWGDTDFDPPYRTLGVIDQGPFYAVKMEAGA
LGTAGGPRTNADAQVVDWNGNPIPGLYAAGNAMAAVLGEIYGGAGGTLGPGLTFGYVAGRHLGSHISNH

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 59512; Mature: 59381

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQQNWDIEADVVVLGSGGAAMTAAIAAHDFGASEVVILERSGMVGGTTAMSGGMLWIP
CCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEC
NNHHMHEEGIEDSDEEVVTYLDSLAPGALDPETLWSFMQNGPEMLRYLAERTPVRLRAFK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEEEEC
DFPDYQPYSPGAKPDGGRSLDNEAFSFERLGKWAGRVNPSKMAYPLRGSLMEAISGSLDE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHCCCCCH
ATLAEREAGDYRGLGQALAGALFMAVLDRNIPVEFEKRARKLVKDGDRVIGVIADDANGK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCC
PFRVRARRGVVIATGGFEWNETLVKAFVRGPLTGPVSVPENEGDGLLMAIEAGAQLGNMQ
CEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHCCCH
NAFWMQSVLEFKPQHRNAKPNYLLGSDERARPGAILVNRAGKRFVNEAANYNALGKSLHA
HHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHH
FDAGTHTYANLPYWLIIDQRYKDKYHTFNSPPGGPTPSFMMKADTLEELAEVAGIDPQGL
HCCCCCEECCCCEEEEEECHHCCHHCCCCCCCCCCCHHHEEEHHHHHHHHHHHCCCCCHH
VATVARFNDMVRKGHDDDFNRGDNSYDNFYMWGDTDFDPPYRTLGVIDQGPFYAVKMEAG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHEEEEECCCCEEEEEEECC
ALGTAGGPRTNADAQVVDWNGNPIPGLYAAGNAMAAVLGEIYGGAGGTLGPGLTFGYVAG
CCCCCCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RHLGSHISNH
HHHHHHHCCC
>Mature Secondary Structure 
SEQQNWDIEADVVVLGSGGAAMTAAIAAHDFGASEVVILERSGMVGGTTAMSGGMLWIP
CCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCEEEEC
NNHHMHEEGIEDSDEEVVTYLDSLAPGALDPETLWSFMQNGPEMLRYLAERTPVRLRAFK
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEEEEC
DFPDYQPYSPGAKPDGGRSLDNEAFSFERLGKWAGRVNPSKMAYPLRGSLMEAISGSLDE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCHHHHHHHHHCCCCCH
ATLAEREAGDYRGLGQALAGALFMAVLDRNIPVEFEKRARKLVKDGDRVIGVIADDANGK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCC
PFRVRARRGVVIATGGFEWNETLVKAFVRGPLTGPVSVPENEGDGLLMAIEAGAQLGNMQ
CEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHCCCH
NAFWMQSVLEFKPQHRNAKPNYLLGSDERARPGAILVNRAGKRFVNEAANYNALGKSLHA
HHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHH
FDAGTHTYANLPYWLIIDQRYKDKYHTFNSPPGGPTPSFMMKADTLEELAEVAGIDPQGL
HCCCCCEECCCCEEEEEECHHCCHHCCCCCCCCCCCHHHEEEHHHHHHHHHHHCCCCCHH
VATVARFNDMVRKGHDDDFNRGDNSYDNFYMWGDTDFDPPYRTLGVIDQGPFYAVKMEAG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHEEEEECCCCEEEEEEECC
ALGTAGGPRTNADAQVVDWNGNPIPGLYAAGNAMAAVLGEIYGGAGGTLGPGLTFGYVAG
CCCCCCCCCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RHLGSHISNH
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]