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The map label for this gene is paaF [C]

Identifier: 87199484

GI number: 87199484

Start: 1505223

End: 1506062

Strand: Reverse

Name: paaF [C]

Synonym: Saro_1464

Alternate gene names: 87199484

Gene position: 1506062-1505223 (Counterclockwise)

Preceding gene: 87199485

Following gene: 87199483

Centisome position: 42.29

GC content: 66.9

Gene sequence:

>840_bases
ATGAGCGGAGACGAGCCCGGCCCGGAACTCCTGATCGAGCATTCCGGTTCGATCAGCTGGATCGTGCTCAACCGCCCGGC
CCAGGCCAACGCGCTGTCGCGCACGCTGATCGAGAGCTTCTCGGCAGCGCTGCGCACGCTCGAGGACGAAGGCGCGCCGG
TCATCGGCATCCGCGCCAACGGCAAGGGTTTCTGCTCCGGCATGGACCTTGGCCAGTACGGCGGCGCCGCCGTTCTCGAT
CCCACCGCCGATTCCAGGCGCCTCAACGAGAACGTCGCGCGCTGGCTGGCGATCTGGGATCATCCCAAGCCGGTCATCGC
TGCGGTCCATGGGTATTGCGCCGGCGTTGCGGCGCAGATGTGCATCTTTGCTGACCTCACCGTGGTGGCAGACAACGTTC
GCATCAGCGAACCGGGCGTGCCCATCGGTGGCGGCTTCATCGCGCCGACATGGGTGGCCCAGGTCGGCGCCAAGCGGGCG
AAGGAATTCGCCTTCCTGCCCGGCAACAGCATCGACGGGCCGACGGCGGTGGAATGGGGCTGGGCCAACCACTGCGTGCC
CGCCGACCGTCTCGTCGAGTCAGTCGAGAGCCTGGCTGCGCGCATGGCCCTGATCCCGTCGGACGTGCTGCGGATCAAAA
AGCTGTCGATCAACCGCGCCGCCGAGGCCGCAGGCTTTCGCACCGCCACCGGTGGCATCGCCGAGATGGACTCGCTGCTG
CATGCCGCACCGTCCGTTCTCGCCATCCGCGAGCGCATGACCACGCTCGGCCTCAAGGCCGTCCTCGACGAATACCGCGG
TCCTTCCTCGACCGCCATCATGAACAGTTTCAAGGAGTAA

Upstream 100 bases:

>100_bases
TCAAGGTCGATGGCCAGTGGAAGTTCAAGCGCCGCGAGCTGCGTACTACGATGACCACCGACTATCACGTGCGCGCTGCC
AACCCCATCGGTCGCGCCGA

Downstream 100 bases:

>100_bases
GCCATGGCCGATATTGACTTCAGGATCGAGGGCCACGTCGCTCACGTCCGCCTCAACCGCCCCCAGGGTCTGAACGCGAT
CACCCAGGAGATGGACGATC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSGDEPGPELLIEHSGSISWIVLNRPAQANALSRTLIESFSAALRTLEDEGAPVIGIRANGKGFCSGMDLGQYGGAAVLD
PTADSRRLNENVARWLAIWDHPKPVIAAVHGYCAGVAAQMCIFADLTVVADNVRISEPGVPIGGGFIAPTWVAQVGAKRA
KEFAFLPGNSIDGPTAVEWGWANHCVPADRLVESVESLAARMALIPSDVLRIKKLSINRAAEAAGFRTATGGIAEMDSLL
HAAPSVLAIRERMTTLGLKAVLDEYRGPSSTAIMNSFKE

Sequences:

>Translated_279_residues
MSGDEPGPELLIEHSGSISWIVLNRPAQANALSRTLIESFSAALRTLEDEGAPVIGIRANGKGFCSGMDLGQYGGAAVLD
PTADSRRLNENVARWLAIWDHPKPVIAAVHGYCAGVAAQMCIFADLTVVADNVRISEPGVPIGGGFIAPTWVAQVGAKRA
KEFAFLPGNSIDGPTAVEWGWANHCVPADRLVESVESLAARMALIPSDVLRIKKLSINRAAEAAGFRTATGGIAEMDSLL
HAAPSVLAIRERMTTLGLKAVLDEYRGPSSTAIMNSFKE
>Mature_278_residues
SGDEPGPELLIEHSGSISWIVLNRPAQANALSRTLIESFSAALRTLEDEGAPVIGIRANGKGFCSGMDLGQYGGAAVLDP
TADSRRLNENVARWLAIWDHPKPVIAAVHGYCAGVAAQMCIFADLTVVADNVRISEPGVPIGGGFIAPTWVAQVGAKRAK
EFAFLPGNSIDGPTAVEWGWANHCVPADRLVESVESLAARMALIPSDVLRIKKLSINRAAEAAGFRTATGGIAEMDSLLH
AAPSVLAIRERMTTLGLKAVLDEYRGPSSTAIMNSFKE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=26.8774703557312, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1787659, Length=220, Percent_Identity=26.8181818181818, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788597, Length=210, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=225, Percent_Identity=28.4444444444444, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=27.3076923076923, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=213, Percent_Identity=27.2300469483568, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=262, Percent_Identity=27.4809160305344, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=27.710843373494, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=27.710843373494, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653139, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29498; Mature: 29367

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDEPGPELLIEHSGSISWIVLNRPAQANALSRTLIESFSAALRTLEDEGAPVIGIRAN
CCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GKGFCSGMDLGQYGGAAVLDPTADSRRLNENVARWLAIWDHPKPVIAAVHGYCAGVAAQM
CCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
CIFADLTVVADNVRISEPGVPIGGGFIAPTWVAQVGAKRAKEFAFLPGNSIDGPTAVEWG
HHHHHHHHEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEECC
WANHCVPADRLVESVESLAARMALIPSDVLRIKKLSINRAAEAAGFRTATGGIAEMDSLL
CCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HAAPSVLAIRERMTTLGLKAVLDEYRGPSSTAIMNSFKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
SGDEPGPELLIEHSGSISWIVLNRPAQANALSRTLIESFSAALRTLEDEGAPVIGIRAN
CCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
GKGFCSGMDLGQYGGAAVLDPTADSRRLNENVARWLAIWDHPKPVIAAVHGYCAGVAAQM
CCEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
CIFADLTVVADNVRISEPGVPIGGGFIAPTWVAQVGAKRAKEFAFLPGNSIDGPTAVEWG
HHHHHHHHEECCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEECC
WANHCVPADRLVESVESLAARMALIPSDVLRIKKLSINRAAEAAGFRTATGGIAEMDSLL
CCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HAAPSVLAIRERMTTLGLKAVLDEYRGPSSTAIMNSFKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA