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The map label for this gene is istB [H]

Identifier: 87199478

GI number: 87199478

Start: 1501401

End: 1502258

Strand: Direct

Name: istB [H]

Synonym: Saro_1458

Alternate gene names: 87199478

Gene position: 1501401-1502258 (Clockwise)

Preceding gene: 87199477

Following gene: 87199479

Centisome position: 42.16

GC content: 64.57

Gene sequence:

>858_bases
ATGAACGGCATCGCACCAGCCACGCGCATCGACTCCATCCGCCGGAGCCTGGTCAGCCTCAAGATGCCCCGGGCGCTCGA
GATCCTCGATGCGACCCTGCGGCGTATCGAGCAGGGCCAGATCGATGGCATCGAGGCACTCGACGAACTGCTGGGCGAAG
AGTTGTCGCTGCGTGAAAACCGGCGGATCAAGGCGGCCCTGCGCATGGCCCGGCTGCCCGTCGTGAAAACGCTCGCCGGC
TACGACTTCTCGTTCCAGCCTTCGCTCGACAGGAACCGTATCCTCGCTCTTGCCGGGCTCGACTTCATTGAACGCGCCGA
GGTCGTCCATCTGCTGGGGCCGCCTGGCACCGGCAAAAGCCACATTGCCACCGCGCTCGCGGTTGAGGCCGTGCGCGCCG
GGAAAGCGGTCTACTTCATCCCGCTCGCCGATCTGATCGCCCAGCTCGCCAAGGCCGAACGTGAAGGCACGCTCAGGGAG
AAGATCCGGTTCCTGACCAGGGCATCGCTGCTCGTCGTCGATGAAATCGGATACCTGCCGGTCACACCGGGCGGCGCCAA
CCTGTTCTTCCAGCTGGTCAACGCCCGCTACGAAAAGGGCGCCATGATCCTCACCTCCAACCGCGGCTTCGCCGAATGGG
GTGAGGTCTTCGGCGATCCTGTCGTCGCCACGGCGCTGCTCGACAGGCTGCTGCACCATGCCGTCGTCATCCAGGTCGAA
GGCTCCAGCTACCGAATGCGTGAGCACGCCGCGCTTGTGCCCGAAAACGTGCGCAACGGTGCCACGTTCCATCCGCCGCA
GCCCAAACGCCGTGGCCGACCACCGACGAAAGGAAAATCCGATCACGAATATGGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGACGACGATCGGCCGCATCGGTGACTATGTGCCACTGCGCTCGCTGGCCATCTATGAAGCGATCGGCGCGGGC
CTTGCCACGGAGGGTCGGCC

Downstream 100 bases:

>100_bases
TCACCGACCGACCTGAACCGCCAGCATAGGGAATTTTGGGAGCCCACATCTGCGGAAACTTCGGTGCCCATTGACAGCTG
ATATCCGCAGGCAGCGGGTC

Product: IstB-like ATP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNGIAPATRIDSIRRSLVSLKMPRALEILDATLRRIEQGQIDGIEALDELLGEELSLRENRRIKAALRMARLPVVKTLAG
YDFSFQPSLDRNRILALAGLDFIERAEVVHLLGPPGTGKSHIATALAVEAVRAGKAVYFIPLADLIAQLAKAEREGTLRE
KIRFLTRASLLVVDEIGYLPVTPGGANLFFQLVNARYEKGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQVE
GSSYRMREHAALVPENVRNGATFHPPQPKRRGRPPTKGKSDHEYG

Sequences:

>Translated_285_residues
MNGIAPATRIDSIRRSLVSLKMPRALEILDATLRRIEQGQIDGIEALDELLGEELSLRENRRIKAALRMARLPVVKTLAG
YDFSFQPSLDRNRILALAGLDFIERAEVVHLLGPPGTGKSHIATALAVEAVRAGKAVYFIPLADLIAQLAKAEREGTLRE
KIRFLTRASLLVVDEIGYLPVTPGGANLFFQLVNARYEKGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQVE
GSSYRMREHAALVPENVRNGATFHPPQPKRRGRPPTKGKSDHEYG
>Mature_285_residues
MNGIAPATRIDSIRRSLVSLKMPRALEILDATLRRIEQGQIDGIEALDELLGEELSLRENRRIKAALRMARLPVVKTLAG
YDFSFQPSLDRNRILALAGLDFIERAEVVHLLGPPGTGKSHIATALAVEAVRAGKAVYFIPLADLIAQLAKAEREGTLRE
KIRFLTRASLLVVDEIGYLPVTPGGANLFFQLVNARYEKGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQVE
GSSYRMREHAALVPENVRNGATFHPPQPKRRGRPPTKGKSDHEYG

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 31407; Mature: 31407

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGIAPATRIDSIRRSLVSLKMPRALEILDATLRRIEQGQIDGIEALDELLGEELSLREN
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHC
RRIKAALRMARLPVVKTLAGYDFSFQPSLDRNRILALAGLDFIERAEVVHLLGPPGTGKS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHCEEEEECCCCCCHH
HIATALAVEAVRAGKAVYFIPLADLIAQLAKAEREGTLREKIRFLTRASLLVVDEIGYLP
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCEE
VTPGGANLFFQLVNARYEKGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQVE
CCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEC
GSSYRMREHAALVPENVRNGATFHPPQPKRRGRPPTKGKSDHEYG
CCCEEHHHHHHHCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGIAPATRIDSIRRSLVSLKMPRALEILDATLRRIEQGQIDGIEALDELLGEELSLREN
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHC
RRIKAALRMARLPVVKTLAGYDFSFQPSLDRNRILALAGLDFIERAEVVHLLGPPGTGKS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHCEEEEECCCCCCHH
HIATALAVEAVRAGKAVYFIPLADLIAQLAKAEREGTLREKIRFLTRASLLVVDEIGYLP
HHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCEE
VTPGGANLFFQLVNARYEKGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQVE
CCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEC
GSSYRMREHAALVPENVRNGATFHPPQPKRRGRPPTKGKSDHEYG
CCCEEHHHHHHHCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]