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The map label for this gene is cisA [H]

Identifier: 87199470

GI number: 87199470

Start: 1494442

End: 1496010

Strand: Reverse

Name: cisA [H]

Synonym: Saro_1449

Alternate gene names: 87199470

Gene position: 1496010-1494442 (Counterclockwise)

Preceding gene: 87199471

Following gene: 87199469

Centisome position: 42.0

GC content: 67.5

Gene sequence:

>1569_bases
ATGAAGACGCTGGCCTGCGCGATCTACACCCGCAAGAGCTCGGATGAAGGGCTCGAACAGGGTTTCAACAGCCTCGATGC
CCAGCGCGAGGCAGGCGAGGCTTATGTAAAGAGCCAGGCGGCCGAGGGTTGGCGGCTGGTTGCCACGCGCTATGACGATG
GCGGGTACTCGGGCGGCACCATGGAGCGCCCCGGGCTCCAGCGCCTGCTTGCCGATATCGAGGCCGGGCGGATCGACGTG
GTGGTGGTCTACAAGATCGATCGCCTGACGCGCAGCCTTGCCGACTTTGCCCGCATCGTCGAGCGCTTCGATGCGCGCGG
GGTGAGCTTCGTCTCGGTCACCCAGGCATTCAACACCACCAGCAGCATGGGCCGGCTCACGCTCAACGTGCTGCTCTCGT
TCGCCCAGTTCGAGCGCGAGGTCACCGGCGAGCGTATCCGCGACAAGATCGCAGCCTCCAAGGCGAAGGGCATGTGGATG
GGAGGGATACCTCCGCTCGGCTACGACCTCCCTGTTCCGGGCAGCCGCACCCTCGTCGTCAACGAAGCCGAGGCGGCGAT
CGTGCGTCACATCTTCGAGCGCTATCTCGCGTGTCGCTCGGTACACCGTCTCGCGTCCGCGCTCGAGGAGGAAGGCGTCT
GCTCCAAGGTCTGGGTGACCGCAGCGGGCAAGTCCCACGGCGGACGCCCTTTCTCGCGCGGCGCGCTCTTCCATCTACTC
GGCAATCGCATCTACCTTGGCGAGATCGTCCACAAGGCCGCCTGTCATCCCGGGGCCCATGCTGCCATCGTCGATCCCGA
CGTCTTTGCCAGGACGCAGGCCATCCGCTTTGGCAACCGGCGCCGGACAACCGCGCCGGAGGGGCAAGGGCCGCGCACTG
CCGGCGCTCTTCTTGCCGGTCGCATTCTCGATGCCGGTGGTCAGCCGATGTCTCCATCGCACTCACGCGGCCGCGGGGGA
AGGATCTACCGCTACTATGTCTCTGCCGCCCTCCAGCAGGGGCGCACTCTGCCGGGCAGTGCGATCCGGCGGCTTCCCGC
TGCGGCGCTCGAAGCCCTCGTCACGGCACGGCTTGGCGCAGCGCTCCCGGCAAGCGATGATCCGCTCGTGCTGCTGCGGC
GCGTGCGCATCGTACCGACGGGTCTGGAACTGTACCTGCCTGTCAGCGCCCGTCGCCAGCTGGCGACGGCATTGCCCGCG
GGGATGGAAGCAAGGCTCGCAGGGCGCGAGCTCGTCCTCTGCGTTCCTGTCGCCTTCCCCTTGCGCGGCGGACAGCGCAG
CGCTGCCGCCGGCCATACCGATGGCGACCGTGACCCAGTCCTGATTGCTGCGCTGCGCAAGGCGCATGGCATGCTGACGC
GTGACGCCGATGGCCATCCGACGATCGTCGCCGCGCCTGCCACGGCCTATGATCGCAAGCTGCTGCGTCTCGCATTTCTG
GCCCCTCACCTCCAGCGCGACATTCTCGCCGGCCGCCAGCCGCGCCACATCAGTCTCGCGTTTCTGATGGCCCGTGACAT
CCCGCCTGCCTGGCAGGCGCAGGTCGAGGCACTTGGCTGGACGTCGTAA

Upstream 100 bases:

>100_bases
GGAACGGCACGCTATACCCCAGCCTCTCGGCTGCCGCGAGCGCGATCGCAGGATCGCGCTGGAACGGGCCACGTTTCTTC
GGCCTCAGGCAACGCCGCCC

Downstream 100 bases:

>100_bases
CACCTGAGTCCCTGTTATCGCGATATCGCGGCCTGATATGGTCGATATCGAGGCCTGTTATGGGCGAATAAATACCCTGT
TACGGCGAATAACAGGGAAA

Product: resolvase-like protein

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKTLACAIYTRKSSDEGLEQGFNSLDAQREAGEAYVKSQAAEGWRLVATRYDDGGYSGGTMERPGLQRLLADIEAGRIDV
VVVYKIDRLTRSLADFARIVERFDARGVSFVSVTQAFNTTSSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKAKGMWM
GGIPPLGYDLPVPGSRTLVVNEAEAAIVRHIFERYLACRSVHRLASALEEEGVCSKVWVTAAGKSHGGRPFSRGALFHLL
GNRIYLGEIVHKAACHPGAHAAIVDPDVFARTQAIRFGNRRRTTAPEGQGPRTAGALLAGRILDAGGQPMSPSHSRGRGG
RIYRYYVSAALQQGRTLPGSAIRRLPAAALEALVTARLGAALPASDDPLVLLRRVRIVPTGLELYLPVSARRQLATALPA
GMEARLAGRELVLCVPVAFPLRGGQRSAAAGHTDGDRDPVLIAALRKAHGMLTRDADGHPTIVAAPATAYDRKLLRLAFL
APHLQRDILAGRQPRHISLAFLMARDIPPAWQAQVEALGWTS

Sequences:

>Translated_522_residues
MKTLACAIYTRKSSDEGLEQGFNSLDAQREAGEAYVKSQAAEGWRLVATRYDDGGYSGGTMERPGLQRLLADIEAGRIDV
VVVYKIDRLTRSLADFARIVERFDARGVSFVSVTQAFNTTSSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKAKGMWM
GGIPPLGYDLPVPGSRTLVVNEAEAAIVRHIFERYLACRSVHRLASALEEEGVCSKVWVTAAGKSHGGRPFSRGALFHLL
GNRIYLGEIVHKAACHPGAHAAIVDPDVFARTQAIRFGNRRRTTAPEGQGPRTAGALLAGRILDAGGQPMSPSHSRGRGG
RIYRYYVSAALQQGRTLPGSAIRRLPAAALEALVTARLGAALPASDDPLVLLRRVRIVPTGLELYLPVSARRQLATALPA
GMEARLAGRELVLCVPVAFPLRGGQRSAAAGHTDGDRDPVLIAALRKAHGMLTRDADGHPTIVAAPATAYDRKLLRLAFL
APHLQRDILAGRQPRHISLAFLMARDIPPAWQAQVEALGWTS
>Mature_522_residues
MKTLACAIYTRKSSDEGLEQGFNSLDAQREAGEAYVKSQAAEGWRLVATRYDDGGYSGGTMERPGLQRLLADIEAGRIDV
VVVYKIDRLTRSLADFARIVERFDARGVSFVSVTQAFNTTSSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKAKGMWM
GGIPPLGYDLPVPGSRTLVVNEAEAAIVRHIFERYLACRSVHRLASALEEEGVCSKVWVTAAGKSHGGRPFSRGALFHLL
GNRIYLGEIVHKAACHPGAHAAIVDPDVFARTQAIRFGNRRRTTAPEGQGPRTAGALLAGRILDAGGQPMSPSHSRGRGG
RIYRYYVSAALQQGRTLPGSAIRRLPAAALEALVTARLGAALPASDDPLVLLRRVRIVPTGLELYLPVSARRQLATALPA
GMEARLAGRELVLCVPVAFPLRGGQRSAAAGHTDGDRDPVLIAALRKAHGMLTRDADGHPTIVAAPATAYDRKLLRLAFL
APHLQRDILAGRQPRHISLAFLMARDIPPAWQAQVEALGWTS

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=110, Percent_Identity=39.0909090909091, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 56385; Mature: 56385

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLACAIYTRKSSDEGLEQGFNSLDAQREAGEAYVKSQAAEGWRLVATRYDDGGYSGGT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
MERPGLQRLLADIEAGRIDVVVVYKIDRLTRSLADFARIVERFDARGVSFVSVTQAFNTT
CCCHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCH
SSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKAKGMWMGGIPPLGYDLPVPGSRTLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEE
NEAEAAIVRHIFERYLACRSVHRLASALEEEGVCSKVWVTAAGKSHGGRPFSRGALFHLL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
GNRIYLGEIVHKAACHPGAHAAIVDPDVFARTQAIRFGNRRRTTAPEGQGPRTAGALLAG
CCEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
RILDAGGQPMSPSHSRGRGGRIYRYYVSAALQQGRTLPGSAIRRLPAAALEALVTARLGA
HHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ALPASDDPLVLLRRVRIVPTGLELYLPVSARRQLATALPAGMEARLAGRELVLCVPVAFP
CCCCCCCHHHHHHHHHEEECCEEEEEEHHHHHHHHHHCCCCCHHHHCCCEEEEEEEEEEC
LRGGQRSAAAGHTDGDRDPVLIAALRKAHGMLTRDADGHPTIVAAPATAYDRKLLRLAFL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHHHHH
APHLQRDILAGRQPRHISLAFLMARDIPPAWQAQVEALGWTS
HHHHHHHHHCCCCCCEEEEEEEHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKTLACAIYTRKSSDEGLEQGFNSLDAQREAGEAYVKSQAAEGWRLVATRYDDGGYSGGT
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
MERPGLQRLLADIEAGRIDVVVVYKIDRLTRSLADFARIVERFDARGVSFVSVTQAFNTT
CCCHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCH
SSMGRLTLNVLLSFAQFEREVTGERIRDKIAASKAKGMWMGGIPPLGYDLPVPGSRTLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEE
NEAEAAIVRHIFERYLACRSVHRLASALEEEGVCSKVWVTAAGKSHGGRPFSRGALFHLL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHH
GNRIYLGEIVHKAACHPGAHAAIVDPDVFARTQAIRFGNRRRTTAPEGQGPRTAGALLAG
CCEEHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
RILDAGGQPMSPSHSRGRGGRIYRYYVSAALQQGRTLPGSAIRRLPAAALEALVTARLGA
HHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ALPASDDPLVLLRRVRIVPTGLELYLPVSARRQLATALPAGMEARLAGRELVLCVPVAFP
CCCCCCCHHHHHHHHHEEECCEEEEEEHHHHHHHHHHCCCCCHHHHCCCEEEEEEEEEEC
LRGGQRSAAAGHTDGDRDPVLIAALRKAHGMLTRDADGHPTIVAAPATAYDRKLLRLAFL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCEEEECCCHHHHHHHHHHHHH
APHLQRDILAGRQPRHISLAFLMARDIPPAWQAQVEALGWTS
HHHHHHHHHCCCCCCEEEEEEEHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]