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The map label for this gene is slt [H]

Identifier: 87199463

GI number: 87199463

Start: 1485464

End: 1487482

Strand: Reverse

Name: slt [H]

Synonym: Saro_1442

Alternate gene names: 87199463

Gene position: 1487482-1485464 (Counterclockwise)

Preceding gene: 87199468

Following gene: 87199462

Centisome position: 41.76

GC content: 65.92

Gene sequence:

>2019_bases
ATGTCCCGCATGGACGTGAAAGCCTTGATCTTGCGTTCCGGCGTCGCAGGCCTCCTGATTCAGGCCCTCGCCGCCTCCAG
CGCAGTGCATGCTAGCGGTGCCGATGGGGCCGATGGCTCGGACTGGGACCGCGCGCGCGCCAATCTGGTGGCCAGCCAGG
GCGGCGCCATGATGAACGCGGTCGAACGGTGGAAACTCCTTTCCAGTTCCAACCGCTTCGGATTTTCGGACTATTCGACG
TTCGTCCTTTCCTATCCCGGCTTTCCGGACGAGGAGAAGCTGCGCCGGTATGCCGAGCTTTCGCTTGAACGCGAGGCAGC
AGACGCGAACCGCCTTGTCGCATATTTCGACAGCAAGCCACCGCTGACCAATCTCGGGAGGGCGCAATATGCCCTGGCGC
TGATGCAGCTTGGCCGGCCCGAAGCGCGCGAAACGGCGCGGGCTGCCTGGCGCGGCGGCCCGATGACCGACAGCGCGGAA
AGCGCGCTTCGTTCTGCCTGGGGCTCCAGCTTCACCCTCGACGACCATGACGCGCGCATGGACGCGTTGCTGTGGGCCGG
GGCGACGGAGCAGGCGTCGCGTCAGTTGGCATGGGTCAGTCCCGCGCGCAAGGGCATCGACACTGCCCGTCTGGCCGCGA
TGCAGGGCGGCGATCCCTTTGCTTCGCTGTCTGGCGTGTCGAGCGAGCAGCTCAACAGCGATCCCGGATTCGTGTTTCAG
CGCGTCCGCCAGTTGCGCAAGGCCGGGCAGGGCCCCGCGGCCCGCGCGCTGCTCGCCAACCGTCCGCTTCTCATCCGCCA
ACCACTCGACCGCGAGAAATGGGTCGAGGAATTGCTCATCAATGCCCGCGCGGCAGCAAGTGCCGGTGACGCGCGCTCCG
CCATGCGGATCGCCGCCGGGATCGACGACGCGTTCTCTCCCGGAGAGGACGTGAGCAAGGCCACTTATGGTCTGCGCGAC
GATTATACCTCGCTCATGTGGCTTGGTGGAACGCAGGCGTGGTTCAACCTCGGCGATGGCGCAAGCGCGGCGCCGCTGTT
CTGGCGCTATGGCGCGGCGGCGCGGACGCCGGGCACGCGTTCGAAGGGCTTCTACTGGGCAGGCAAGGCGGCGGCTCGAG
CAGGTCTTCAGGCCGATGCGCGGCACTATTTCGAAATGGCCGCGCAATACGGCGACCAGTTCTACGGCCTGCTGGCACTG
GAGCGCCTGGGGCGCCCCGTGCCGAAATACGCGACCGATGCCACCTCCCAGATTTCTGCTGAAGAACGTAGCCGTTTCTA
CTCCCGCCCCCTCACCCAGGCCGTGCGCGAAGTGGCGCGCAACGGAGATTGGCGCACGACGGTCCGCTTCTTCAAGGAAA
TCGCCGAGCAGCAGCAGAGCGAGGCGCAGCACATGATGGTGGCGCAACTCGCGCGCGATCTGGGGCGGCGCGATCTCGGC
GTGATCGTCGGGCAGGCAGCGGCGGCGCGCGGCTACCTCGATTTCCAGCACATCGCCTTCCCCCTGATCCCCGTACCTCA
GGGATACGAGACCAAGTGGACGATGATCCACGCGATCAGCCGGCAGGAAAGCCAGTTCGCGCAGAACGCGGTGAGCCACG
CCGGCGCGCGCGGCCTCATGCAGCTCATGCCGGGCACCGCAAACGAGCAGGCCGGAAAGCTTGGCCTCTCGTACGATGCC
TCCGCGCTGACCGAGGACGCCGGGTACAACATCAGGCTGGGCTCGGGCTATTTCCAGCGCATGCTCGACTACTACGGCGG
GTCGTGGCCGCTGGCCGTGGCAGCCTATAATGCAGGGCCCGGCAACGTGAACAAATGGCTGCGCGCCAACGGCGACCCCC
GCAACGGTGGTATCGACTGGATCGAATGGATGGAACGCATCCCGTTGTCCGAGACGCGCAATTACGTGCAGCGCGTACTG
GAAAACGCGGTGGTCTACGAAGCGATGAATCCGCAGTACGCCAGCTATCGCGGGGCAAATCCGATGAGCTACTTCATCGG
CAAGCGGGAGCCGGGCTGA

Upstream 100 bases:

>100_bases
ATGGACAATATCCCTCAATTTGCCGCATGGTTGCTTCGGTGCATCGCGCACTTTGCCCACGGGGGTCTGTTCAGCGCCTG
ATAAGGAGCCGCTCCATAGT

Downstream 100 bases:

>100_bases
CCCCCAGCCCGGAACGGCGTCCAGGCAAGAGAAGGCCCATGCGCAGCTTAGCGCCGGATAAGCCTTGTCGCCCCTTCCAT
CCTCGCCCTAAAGCCCTGCC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MSRMDVKALILRSGVAGLLIQALAASSAVHASGADGADGSDWDRARANLVASQGGAMMNAVERWKLLSSSNRFGFSDYST
FVLSYPGFPDEEKLRRYAELSLEREAADANRLVAYFDSKPPLTNLGRAQYALALMQLGRPEARETARAAWRGGPMTDSAE
SALRSAWGSSFTLDDHDARMDALLWAGATEQASRQLAWVSPARKGIDTARLAAMQGGDPFASLSGVSSEQLNSDPGFVFQ
RVRQLRKAGQGPAARALLANRPLLIRQPLDREKWVEELLINARAAASAGDARSAMRIAAGIDDAFSPGEDVSKATYGLRD
DYTSLMWLGGTQAWFNLGDGASAAPLFWRYGAAARTPGTRSKGFYWAGKAAARAGLQADARHYFEMAAQYGDQFYGLLAL
ERLGRPVPKYATDATSQISAEERSRFYSRPLTQAVREVARNGDWRTTVRFFKEIAEQQQSEAQHMMVAQLARDLGRRDLG
VIVGQAAAARGYLDFQHIAFPLIPVPQGYETKWTMIHAISRQESQFAQNAVSHAGARGLMQLMPGTANEQAGKLGLSYDA
SALTEDAGYNIRLGSGYFQRMLDYYGGSWPLAVAAYNAGPGNVNKWLRANGDPRNGGIDWIEWMERIPLSETRNYVQRVL
ENAVVYEAMNPQYASYRGANPMSYFIGKREPG

Sequences:

>Translated_672_residues
MSRMDVKALILRSGVAGLLIQALAASSAVHASGADGADGSDWDRARANLVASQGGAMMNAVERWKLLSSSNRFGFSDYST
FVLSYPGFPDEEKLRRYAELSLEREAADANRLVAYFDSKPPLTNLGRAQYALALMQLGRPEARETARAAWRGGPMTDSAE
SALRSAWGSSFTLDDHDARMDALLWAGATEQASRQLAWVSPARKGIDTARLAAMQGGDPFASLSGVSSEQLNSDPGFVFQ
RVRQLRKAGQGPAARALLANRPLLIRQPLDREKWVEELLINARAAASAGDARSAMRIAAGIDDAFSPGEDVSKATYGLRD
DYTSLMWLGGTQAWFNLGDGASAAPLFWRYGAAARTPGTRSKGFYWAGKAAARAGLQADARHYFEMAAQYGDQFYGLLAL
ERLGRPVPKYATDATSQISAEERSRFYSRPLTQAVREVARNGDWRTTVRFFKEIAEQQQSEAQHMMVAQLARDLGRRDLG
VIVGQAAAARGYLDFQHIAFPLIPVPQGYETKWTMIHAISRQESQFAQNAVSHAGARGLMQLMPGTANEQAGKLGLSYDA
SALTEDAGYNIRLGSGYFQRMLDYYGGSWPLAVAAYNAGPGNVNKWLRANGDPRNGGIDWIEWMERIPLSETRNYVQRVL
ENAVVYEAMNPQYASYRGANPMSYFIGKREPG
>Mature_671_residues
SRMDVKALILRSGVAGLLIQALAASSAVHASGADGADGSDWDRARANLVASQGGAMMNAVERWKLLSSSNRFGFSDYSTF
VLSYPGFPDEEKLRRYAELSLEREAADANRLVAYFDSKPPLTNLGRAQYALALMQLGRPEARETARAAWRGGPMTDSAES
ALRSAWGSSFTLDDHDARMDALLWAGATEQASRQLAWVSPARKGIDTARLAAMQGGDPFASLSGVSSEQLNSDPGFVFQR
VRQLRKAGQGPAARALLANRPLLIRQPLDREKWVEELLINARAAASAGDARSAMRIAAGIDDAFSPGEDVSKATYGLRDD
YTSLMWLGGTQAWFNLGDGASAAPLFWRYGAAARTPGTRSKGFYWAGKAAARAGLQADARHYFEMAAQYGDQFYGLLALE
RLGRPVPKYATDATSQISAEERSRFYSRPLTQAVREVARNGDWRTTVRFFKEIAEQQQSEAQHMMVAQLARDLGRRDLGV
IVGQAAAARGYLDFQHIAFPLIPVPQGYETKWTMIHAISRQESQFAQNAVSHAGARGLMQLMPGTANEQAGKLGLSYDAS
ALTEDAGYNIRLGSGYFQRMLDYYGGSWPLAVAAYNAGPGNVNKWLRANGDPRNGGIDWIEWMERIPLSETRNYVQRVLE
NAVVYEAMNPQYASYRGANPMSYFIGKREPG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=291, Percent_Identity=30.5841924398625, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73574; Mature: 73443

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRMDVKALILRSGVAGLLIQALAASSAVHASGADGADGSDWDRARANLVASQGGAMMNA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
VERWKLLSSSNRFGFSDYSTFVLSYPGFPDEEKLRRYAELSLEREAADANRLVAYFDSKP
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PLTNLGRAQYALALMQLGRPEARETARAAWRGGPMTDSAESALRSAWGSSFTLDDHDARM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
DALLWAGATEQASRQLAWVSPARKGIDTARLAAMQGGDPFASLSGVSSEQLNSDPGFVFQ
HHHHCCCCCHHHHCCCHHHCHHHHCCCHHHHHHHCCCCCCHHHCCCCHHHCCCCCCHHHH
RVRQLRKAGQGPAARALLANRPLLIRQPLDREKWVEELLINARAAASAGDARSAMRIAAG
HHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
IDDAFSPGEDVSKATYGLRDDYTSLMWLGGTQAWFNLGDGASAAPLFWRYGAAARTPGTR
CCCCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHEECCCCCCCCCHHHHCCCCCCCCCCC
SKGFYWAGKAAARAGLQADARHYFEMAAQYGDQFYGLLALERLGRPVPKYATDATSQISA
CCCEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHH
EERSRFYSRPLTQAVREVARNGDWRTTVRFFKEIAEQQQSEAQHMMVAQLARDLGRRDLG
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VIVGQAAAARGYLDFQHIAFPLIPVPQGYETKWTMIHAISRQESQFAQNAVSHAGARGLM
HHHHHHHHHHCCCCHHHHHEECEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMPGTANEQAGKLGLSYDASALTEDAGYNIRLGSGYFQRMLDYYGGSWPLAVAAYNAGP
HHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCC
GNVNKWLRANGDPRNGGIDWIEWMERIPLSETRNYVQRVLENAVVYEAMNPQYASYRGAN
CCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
PMSYFIGKREPG
CHHHHCCCCCCC
>Mature Secondary Structure 
SRMDVKALILRSGVAGLLIQALAASSAVHASGADGADGSDWDRARANLVASQGGAMMNA
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
VERWKLLSSSNRFGFSDYSTFVLSYPGFPDEEKLRRYAELSLEREAADANRLVAYFDSKP
HHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PLTNLGRAQYALALMQLGRPEARETARAAWRGGPMTDSAESALRSAWGSSFTLDDHDARM
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
DALLWAGATEQASRQLAWVSPARKGIDTARLAAMQGGDPFASLSGVSSEQLNSDPGFVFQ
HHHHCCCCCHHHHCCCHHHCHHHHCCCHHHHHHHCCCCCCHHHCCCCHHHCCCCCCHHHH
RVRQLRKAGQGPAARALLANRPLLIRQPLDREKWVEELLINARAAASAGDARSAMRIAAG
HHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
IDDAFSPGEDVSKATYGLRDDYTSLMWLGGTQAWFNLGDGASAAPLFWRYGAAARTPGTR
CCCCCCCCCHHHHHHCCCCCCCCCEEEECCCHHHEECCCCCCCCCHHHHCCCCCCCCCCC
SKGFYWAGKAAARAGLQADARHYFEMAAQYGDQFYGLLALERLGRPVPKYATDATSQISA
CCCEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHH
EERSRFYSRPLTQAVREVARNGDWRTTVRFFKEIAEQQQSEAQHMMVAQLARDLGRRDLG
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VIVGQAAAARGYLDFQHIAFPLIPVPQGYETKWTMIHAISRQESQFAQNAVSHAGARGLM
HHHHHHHHHHCCCCHHHHHEECEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLMPGTANEQAGKLGLSYDASALTEDAGYNIRLGSGYFQRMLDYYGGSWPLAVAAYNAGP
HHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCEEEEEECCCC
GNVNKWLRANGDPRNGGIDWIEWMERIPLSETRNYVQRVLENAVVYEAMNPQYASYRGAN
CCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
PMSYFIGKREPG
CHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]