Click here to switch to the map view.

The map label for this gene is greB [H]

Identifier: 87199462

GI number: 87199462

Start: 1484877

End: 1485425

Strand: Reverse

Name: greB [H]

Synonym: Saro_1441

Alternate gene names: 87199462

Gene position: 1485425-1484877 (Counterclockwise)

Preceding gene: 87199463

Following gene: 87199461

Centisome position: 41.71

GC content: 64.48

Gene sequence:

>549_bases
ATGCGCAGCTTAGCGCCGGATAAGCCTTGTCGCCCCTTCCATCCTCGCCCTAAAGCCCTGCCCATGAAGCCTGACGGACA
TCCCATTACGCCTGCCGGATTTGCCGCCCTGCGCGCCCGCTACGACCATCTGCTTGGCACCGAACGCCCTGCGATCGTCG
AGATCGTCAGTTGGGCGGCGGGGAATGGCGACCGGAGCGAGAACGGCGATTATCTTTATGGGCGCAAGCGGATGCGCGAG
ATCGACCGGGAACTGGCGTTCCTCGCGCGGCGCATGAAGGCGTCTCGCGTGGTGGACCCTGCCCGTCAGGAAGACCGCAG
CCGCGTGTTGTTCGGCGCGACCGTCGAGATCGCCGACGAGGACGACAATCGAAAGGTGCTGACGCTGGTGGGTGACGACG
AACAAGATGCATCCAAGGGCCTGATCGGCTGGAGCGCCCCCATCGCCAAGGCGCTGCGCGGAAGCCGGGTGGGCGACCTG
CGCATCGTGCGGCTCCCTTCGGGCCAGAAGGAGTGGGAAATAATGGCGATTTCCTATCCGGAAGCTTGA

Upstream 100 bases:

>100_bases
ACGCCAGCTATCGCGGGGCAAATCCGATGAGCTACTTCATCGGCAAGCGGGAGCCGGGCTGACCCCCAGCCCGGAACGGC
GTCCAGGCAAGAGAAGGCCC

Downstream 100 bases:

>100_bases
GGTTAAACCATCCCTCCATGACAAGCGGTCGCTTCTACATCATGCGCCACGGCGAGACGGTCTACAACGCCACCCGCCGC
CTTCAGGCCAACCACATCCA

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MRSLAPDKPCRPFHPRPKALPMKPDGHPITPAGFAALRARYDHLLGTERPAIVEIVSWAAGNGDRSENGDYLYGRKRMRE
IDRELAFLARRMKASRVVDPARQEDRSRVLFGATVEIADEDDNRKVLTLVGDDEQDASKGLIGWSAPIAKALRGSRVGDL
RIVRLPSGQKEWEIMAISYPEA

Sequences:

>Translated_182_residues
MRSLAPDKPCRPFHPRPKALPMKPDGHPITPAGFAALRARYDHLLGTERPAIVEIVSWAAGNGDRSENGDYLYGRKRMRE
IDRELAFLARRMKASRVVDPARQEDRSRVLFGATVEIADEDDNRKVLTLVGDDEQDASKGLIGWSAPIAKALRGSRVGDL
RIVRLPSGQKEWEIMAISYPEA
>Mature_182_residues
MRSLAPDKPCRPFHPRPKALPMKPDGHPITPAGFAALRARYDHLLGTERPAIVEIVSWAAGNGDRSENGDYLYGRKRMRE
IDRELAFLARRMKASRVVDPARQEDRSRVLFGATVEIADEDDNRKVLTLVGDDEQDASKGLIGWSAPIAKALRGSRVGDL
RIVRLPSGQKEWEIMAISYPEA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=39.7350993377483, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=30.718954248366, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20327; Mature: 20327

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLAPDKPCRPFHPRPKALPMKPDGHPITPAGFAALRARYDHLLGTERPAIVEIVSWAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GNGDRSENGDYLYGRKRMREIDRELAFLARRMKASRVVDPARQEDRSRVLFGATVEIADE
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEEEEEECC
DDNRKVLTLVGDDEQDASKGLIGWSAPIAKALRGSRVGDLRIVRLPSGQKEWEIMAISYP
CCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECC
EA
CC
>Mature Secondary Structure
MRSLAPDKPCRPFHPRPKALPMKPDGHPITPAGFAALRARYDHLLGTERPAIVEIVSWAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GNGDRSENGDYLYGRKRMREIDRELAFLARRMKASRVVDPARQEDRSRVLFGATVEIADE
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEEEEEEECC
DDNRKVLTLVGDDEQDASKGLIGWSAPIAKALRGSRVGDLRIVRLPSGQKEWEIMAISYP
CCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEECC
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]