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The map label for this gene is yogA [H]

Identifier: 87199452

GI number: 87199452

Start: 1473435

End: 1474448

Strand: Reverse

Name: yogA [H]

Synonym: Saro_1431

Alternate gene names: 87199452

Gene position: 1474448-1473435 (Counterclockwise)

Preceding gene: 87199453

Following gene: 87199446

Centisome position: 41.4

GC content: 64.69

Gene sequence:

>1014_bases
ATGACCGTCAATGCAATTCGCGTAGGCTCCCCCGCCTCGCTCGACACCTTGTATCTCGATACGCTGCCTGATTGCGGACA
ACCCGGACCTGGCGAAATCCGGGTCCGGCTGCGCGCCTCGTCGCTCAACTTCCACGACTACGCGGTGGTTACCGGGATGC
TGCCGGCCGCCGCAGGCCGAATTCCCATGTCGGACGGCGCCGGAGAGGTCCTCGGGGTGGGCGATGGCGTGACCGAATTC
AAGGCGGGCGACGCAGTCGTCTCGACCTTCTTCACCGACTGGGACGCCGGTCCGCCAAAGTCCGCCGCGTTCACGACCGT
CCCTGGAGACGGCATCGATGGCTACGCGCGGGAAGAAGTGGTGGCCCCGGCTCACTGGTTCACCCGCGCGCCCTCAGGCT
ACAGTCACGCAGAAGCCGCCACGCTGACCTGCGCGGGCCTTACGGCATGGCGCGCCCTGTTCGTAGACAACGCGATCAAG
CCGGGCGACACGGTCCTCGTGCAAGGCACTGGCGGCGTCTCGGTTTTCGCGCTGCAGTTGGCAAAGGCGGCAGGCGCGCG
TGTCATCGCCACGAGTTCCTCCGACGAGAAGCTGGAACGCCTGCGCAGCCTCGGAGCGGATGAAACCATCAACTACAAGA
CGCAAACCGCCTGGGGGATGAAGGCACTCGATCTCACTGCCGGCATCGGCGTCGACTGTGTCGTCGAGATCGGCGGGGCC
GGTACGCTTGACCAGTCGATGATGGCCACCCGCGTGGGTGGACACATCGCGCTGATCGGCGTTCTCGCGGGATTTGCGGG
TCCCGTTCAAACCGCGTTGCTGATGGCCAAGAATCTGCGCGTCCAAGGCCTTACCGTGGCCTCGCGTGCCGATCACCTGC
GAATGATCGCCGGTATCGAGGCCAACGGGATCAAGCCTGTCATCGACCGCCACTTTCCACTTGCCGATCTTGCCGGAGCC
TTCCGCCATCAGGAGAGCGGCAGGCATTTCGGCAAGATCGTCATCGACATCTGA

Upstream 100 bases:

>100_bases
GCGGAAAGGTGACCTTCCGCAACGGCGAATGGACGGGTGCCACGCCGGGCAACCTCATTCGCGGACCACAGCGCGCCGAT
CTCATGGAGGCTGCCGAATA

Downstream 100 bases:

>100_bases
CGCACTTCTCAGGCCGCGCGGCGCAGGCCTCTCTGGCCCGCGCCGCCGCCTTGGCCTTCCAGGCGAAATTGCGAGACGAG
CGAGGATAGGCGGCTTGCCT

Product: alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTVNAIRVGSPASLDTLYLDTLPDCGQPGPGEIRVRLRASSLNFHDYAVVTGMLPAAAGRIPMSDGAGEVLGVGDGVTEF
KAGDAVVSTFFTDWDAGPPKSAAFTTVPGDGIDGYAREEVVAPAHWFTRAPSGYSHAEAATLTCAGLTAWRALFVDNAIK
PGDTVLVQGTGGVSVFALQLAKAAGARVIATSSSDEKLERLRSLGADETINYKTQTAWGMKALDLTAGIGVDCVVEIGGA
GTLDQSMMATRVGGHIALIGVLAGFAGPVQTALLMAKNLRVQGLTVASRADHLRMIAGIEANGIKPVIDRHFPLADLAGA
FRHQESGRHFGKIVIDI

Sequences:

>Translated_337_residues
MTVNAIRVGSPASLDTLYLDTLPDCGQPGPGEIRVRLRASSLNFHDYAVVTGMLPAAAGRIPMSDGAGEVLGVGDGVTEF
KAGDAVVSTFFTDWDAGPPKSAAFTTVPGDGIDGYAREEVVAPAHWFTRAPSGYSHAEAATLTCAGLTAWRALFVDNAIK
PGDTVLVQGTGGVSVFALQLAKAAGARVIATSSSDEKLERLRSLGADETINYKTQTAWGMKALDLTAGIGVDCVVEIGGA
GTLDQSMMATRVGGHIALIGVLAGFAGPVQTALLMAKNLRVQGLTVASRADHLRMIAGIEANGIKPVIDRHFPLADLAGA
FRHQESGRHFGKIVIDI
>Mature_336_residues
TVNAIRVGSPASLDTLYLDTLPDCGQPGPGEIRVRLRASSLNFHDYAVVTGMLPAAAGRIPMSDGAGEVLGVGDGVTEFK
AGDAVVSTFFTDWDAGPPKSAAFTTVPGDGIDGYAREEVVAPAHWFTRAPSGYSHAEAATLTCAGLTAWRALFVDNAIKP
GDTVLVQGTGGVSVFALQLAKAAGARVIATSSSDEKLERLRSLGADETINYKTQTAWGMKALDLTAGIGVDCVVEIGGAG
TLDQSMMATRVGGHIALIGVLAGFAGPVQTALLMAKNLRVQGLTVASRADHLRMIAGIEANGIKPVIDRHFPLADLAGAF
RHQESGRHFGKIVIDI

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=351, Percent_Identity=25.9259259259259, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI22538444, Length=351, Percent_Identity=25.9259259259259, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI194239674, Length=310, Percent_Identity=26.1290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI13236495, Length=310, Percent_Identity=26.1290322580645, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI18379349, Length=335, Percent_Identity=25.9701492537313, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI28557745, Length=244, Percent_Identity=31.9672131147541, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI47519420, Length=353, Percent_Identity=27.4787535410765, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI194239676, Length=328, Percent_Identity=24.6951219512195, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI41872631, Length=330, Percent_Identity=26.969696969697, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI67078404, Length=276, Percent_Identity=27.1739130434783, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081918, Length=315, Percent_Identity=28.2539682539683, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790485, Length=256, Percent_Identity=28.515625, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790045, Length=325, Percent_Identity=24.9230769230769, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=331, Percent_Identity=27.7945619335347, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=322, Percent_Identity=25.776397515528, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=322, Percent_Identity=25.1552795031056, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=324, Percent_Identity=24.6913580246914, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=322, Percent_Identity=25.1552795031056, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319621, Length=327, Percent_Identity=26.605504587156, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322619, Length=335, Percent_Identity=25.6716417910448, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319955, Length=335, Percent_Identity=25.6716417910448, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581345, Length=224, Percent_Identity=29.9107142857143, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 34993; Mature: 34862

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNAIRVGSPASLDTLYLDTLPDCGQPGPGEIRVRLRASSLNFHDYAVVTGMLPAAAGR
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCC
IPMSDGAGEVLGVGDGVTEFKAGDAVVSTFFTDWDAGPPKSAAFTTVPGDGIDGYAREEV
CCCCCCCCCEEECCCCCHHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHE
VAPAHWFTRAPSGYSHAEAATLTCAGLTAWRALFVDNAIKPGDTVLVQGTGGVSVFALQL
ECCHHHHHCCCCCCCCCCHHEEEEHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
AKAAGARVIATSSSDEKLERLRSLGADETINYKTQTAWGMKALDLTAGIGVDCVVEIGGA
HHHCCCEEEEECCCHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEECCC
GTLDQSMMATRVGGHIALIGVLAGFAGPVQTALLMAKNLRVQGLTVASRADHLRMIAGIE
CCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCEEEEECCC
ANGIKPVIDRHFPLADLAGAFRHQESGRHFGKIVIDI
CCCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEC
>Mature Secondary Structure 
TVNAIRVGSPASLDTLYLDTLPDCGQPGPGEIRVRLRASSLNFHDYAVVTGMLPAAAGR
CEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCC
IPMSDGAGEVLGVGDGVTEFKAGDAVVSTFFTDWDAGPPKSAAFTTVPGDGIDGYAREEV
CCCCCCCCCEEECCCCCHHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHE
VAPAHWFTRAPSGYSHAEAATLTCAGLTAWRALFVDNAIKPGDTVLVQGTGGVSVFALQL
ECCHHHHHCCCCCCCCCCHHEEEEHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
AKAAGARVIATSSSDEKLERLRSLGADETINYKTQTAWGMKALDLTAGIGVDCVVEIGGA
HHHCCCEEEEECCCHHHHHHHHHCCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEECCC
GTLDQSMMATRVGGHIALIGVLAGFAGPVQTALLMAKNLRVQGLTVASRADHLRMIAGIE
CCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCEEEEECCC
ANGIKPVIDRHFPLADLAGAFRHQESGRHFGKIVIDI
CCCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]