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The map label for this gene is bioD

Identifier: 87198741

GI number: 87198741

Start: 756948

End: 757568

Strand: Reverse

Name: bioD

Synonym: Saro_0717

Alternate gene names: 87198741

Gene position: 757568-756948 (Counterclockwise)

Preceding gene: 87198742

Following gene: 87198735

Centisome position: 21.27

GC content: 67.95

Gene sequence:

>621_bases
ATGACCGCTTTCGTCGTCACCGGCACAGACACGGGCGTCGGCAAGACGATCTTTTCCGCCGCGCTGACAGGCGCGCTTAA
CGCGCATTACTGGAAGCCTGTCCAGGCCGGGCTCGAAGACGGCGCAGACCGCGATCACGTGGCACGGCTCGCCGGGGTTC
CCGCCAGCCATGTCCTGCCCGAATCCTACCGCCTCAACACGCCTTGCTCTCCCCACCGCGCGGCGGAGCTTGACGGCGTG
GTGCTGGACCTGGCCCGCATCGCACTTCCGGACGTCCGGCCACTGGTAGTCGAAGGTGCGGGCGGCGCGCTCGTTCCCGT
CACCCGCAACACGACCTATGCGGACGTCTTCGCCTGGTGGAACCTGCCGGTCGTGATCGTTGCCCGCACCGGGCTTGGTA
CGATCAATCACTCGCTCCTGACGATCGAGGCGCTGCGCGCGCGCGGGGTGCCGATCCACGGCGTAGCCTTCGTCGGCGAC
GCAGTGGAGGACAGCGAGGCCACGATCTGCGCCATGGGCGAAGTCAGGCGGTTAGGCCGCCTGCCGATGCTCGGCCAGCT
CGACCGGCCCGCGCTCGCACAGGCCTTTGCCGAAGGCTTCCGGGTAGAAGATTTCGCCTAG

Upstream 100 bases:

>100_bases
CAGTGCCGCAAGGCACCAGCCGCCTGCGCATCTCGCTGACGCTCAACGCCACGCGCGACGACGTCGATGCGCTTGGCACA
GCACTGGAAGAGGCGCTGCG

Downstream 100 bases:

>100_bases
GACGGGCCCCGCCCGCTCAATCCTCGAAGATCGCCACGGCCCGGGTCCAGCCGGCGGATGCACCCTTGATCTTGTGGGGG
AAGCAACTGACCGTGAAGCC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTAFVVTGTDTGVGKTIFSAALTGALNAHYWKPVQAGLEDGADRDHVARLAGVPASHVLPESYRLNTPCSPHRAAELDGV
VLDLARIALPDVRPLVVEGAGGALVPVTRNTTYADVFAWWNLPVVIVARTGLGTINHSLLTIEALRARGVPIHGVAFVGD
AVEDSEATICAMGEVRRLGRLPMLGQLDRPALAQAFAEGFRVEDFA

Sequences:

>Translated_206_residues
MTAFVVTGTDTGVGKTIFSAALTGALNAHYWKPVQAGLEDGADRDHVARLAGVPASHVLPESYRLNTPCSPHRAAELDGV
VLDLARIALPDVRPLVVEGAGGALVPVTRNTTYADVFAWWNLPVVIVARTGLGTINHSLLTIEALRARGVPIHGVAFVGD
AVEDSEATICAMGEVRRLGRLPMLGQLDRPALAQAFAEGFRVEDFA
>Mature_205_residues
TAFVVTGTDTGVGKTIFSAALTGALNAHYWKPVQAGLEDGADRDHVARLAGVPASHVLPESYRLNTPCSPHRAAELDGVV
LDLARIALPDVRPLVVEGAGGALVPVTRNTTYADVFAWWNLPVVIVARTGLGTINHSLLTIEALRARGVPIHGVAFVGDA
VEDSEATICAMGEVRRLGRLPMLGQLDRPALAQAFAEGFRVEDFA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=208, Percent_Identity=31.25, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324385, Length=182, Percent_Identity=38.4615384615385, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_NOVAD (Q2GAF9)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495998.1
- ProteinModelPortal:   Q2GAF9
- SMR:   Q2GAF9
- STRING:   Q2GAF9
- GeneID:   3918541
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0717
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   WKPIQSG
- PhylomeDB:   Q2GAF9
- ProtClustDB:   PRK00090
- BioCyc:   NARO279238:SARO_0717-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 21783; Mature: 21652

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFVVTGTDTGVGKTIFSAALTGALNAHYWKPVQAGLEDGADRDHVARLAGVPASHVLP
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHCCC
ESYRLNTPCSPHRAAELDGVVLDLARIALPDVRPLVVEGAGGALVPVTRNTTYADVFAWW
CCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEECC
NLPVVIVARTGLGTINHSLLTIEALRARGVPIHGVAFVGDAVEDSEATICAMGEVRRLGR
CCCEEEEEECCCCCCCHHHEEEEHHHHCCCCEEEHEEECCCCCCCCCEEEEHHHHHHHCC
LPMLGQLDRPALAQAFAEGFRVEDFA
CCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAFVVTGTDTGVGKTIFSAALTGALNAHYWKPVQAGLEDGADRDHVARLAGVPASHVLP
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHCCC
ESYRLNTPCSPHRAAELDGVVLDLARIALPDVRPLVVEGAGGALVPVTRNTTYADVFAWW
CCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEECC
NLPVVIVARTGLGTINHSLLTIEALRARGVPIHGVAFVGDAVEDSEATICAMGEVRRLGR
CCCEEEEEECCCCCCCHHHEEEEHHHHCCCCEEEHEEECCCCCCCCCEEEEHHHHHHHCC
LPMLGQLDRPALAQAFAEGFRVEDFA
CCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA