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The map label for this gene is groEL

Identifier: 87198061

GI number: 87198061

Start: 35710

End: 37353

Strand: Direct

Name: groEL

Synonym: Saro_0035

Alternate gene names: 87198061

Gene position: 35710-37353 (Clockwise)

Preceding gene: 87198060

Following gene: 87198062

Centisome position: 1.0

GC content: 64.84

Gene sequence:

>1644_bases
ATGGCTGCCAAGGACGTAAAGTTCGGCCGCGACGCTCGTGAACGCATTCTGCGCGGCGTCGACATCCTCGCTGATGCCGT
GAAGGTCACGCTGGGCCCCAAGGGCCGCAACGTCGTGATCGACAAGAGCTTCGGCGCCCCCCGCATCACCAAGGACGGTG
TTTCGGTCGCCAAGGAAATCGAGCTCAAGGACAAGTTCGAGAACATGGGCGCCCAGATGCTGCGCGAAGTGGCCTCGAAG
GCCAACGACGCGGCCGGTGACGGCACCACCACCGCGACCGTTCTTGCACAGGCGATCGTTCGCGAAGGCATGACCGCGGT
TGCCGCCGGCATGAACCCGATGGACCTCAAGCGCGGCATCGACATCGCCGTCGGCAAGGTCGTCGAGAACCTCAAGGCCC
GTTCGACCCCGGTCGCCGGTTCGTCGGAAATCGCCCAGGTCGGCATCATCTCGGCCAACGGCGACACCGAAGTCGGCCAG
AAGATCGCCGAGGCGATGGAGAAGGTCGGCAAGGAAGGCGTGATCACCGTTGAAGAGGCCAAGGGCCTCGAATTCGAACT
CGATGTCGTCGAAGGCATGCAGTTCGACCGCGGCTACCTCTCGCCCTACTTCATCACCAACCCGGAAAAGATGACGGTCG
AACTCGAGAACCCGTACATCCTGATCCACGAGAAGAAGCTGTCGTCGCTCCAGGCGATGCTGCCGATCCTCGAAGCCGTG
GTGCAGTCGGGCCGTCCGCTCCTCATCATCGCCGAGGACATCGAAGGCGAAGCGCTGGCCACCCTCGTGGTCAACAAGCT
GCGCGGTGGCCTCAAGATTGCCGCCGTCAAGGCTCCGGGCTTCGGTGACCGCCGCAAGGCCATGCTGGGCGACATCGCCA
CGCTGACCGCCGGCGAAATGATCTCCGAAGACCTCGGCATCAAGCTGGAGAGCGTCACGCTCGCCATGCTCGGCCAGGCC
AAGAAGGTCACCATCGACAAGGACAACACCACGATCGTCGACGGCGCCGGTTCGGCCGAAGAGATCAAGGCCCGCGTCGA
GCAGATCCGTGCGCAGATCGAAGTCACCACTTCGGACTACGACCGCGAGAAGCTGCAGGAACGCCTTGCCAAGCTTGCTG
GCGGCGTTGCCGTGATCAAGGTCGGCGGCGCGACCGAAGTCGAGGTCAAGGAGCGCAAGGACCGCGTCGACGACGCTCTC
CACGCCACCCGCGCCGCAGTCGAGGAAGGTATCGTCCCGGGCGGCGGTACGGCTCTGCTCTATGCCACCAAGGCTCTCGA
AGGCCTCAAGGGCGCCAACGACGACCAGACCAAGGGCATCGACATCGTGCGCCGCGCGATCCAGGCCCCGATCCGTCAGA
TCGCCGCGAACGCGGGTCATGACGGTGCGGTCGTCTCGGGCAACCTGCTGCGCGAGAACGACGAGAACCAGGGCTTCAAC
GCCGCGACCGACACCTACGAGAACCTGAAGGCCGCCGGCGTCATCGACCCGACCAAGGTCGTGCGCACCGCGCTTCAGGA
CGCTGCCTCGGTCTCGGGCCTGCTGATCACCACGGAAGCGGCGATCAGCGAGAAGCCGGACGACAAGCCCGCGATGCCGC
CGATGGGCGGTGGAATGGGCGGCATGGGCGGCATGGACTTCTAA

Upstream 100 bases:

>100_bases
GAAGACCTGCTCATCATGAAGGAATCGGACATCCTCGGCGTGATCGGCTGATCGACGCTGCTGTCCTGAAATCCACTCAA
GAAATTCGAAGGAACAAGAC

Downstream 100 bases:

>100_bases
GTCCACGCAGCTACCGCTTGAGAAACGAGGCCGGAAGGATCAGTCCTTCCGGCCTCTTTCCTTATCCGGACGCGCCGGTT
CCCGCCGGGAACCAACTGCG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKDVKFGRDARERILRGVDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELKDKFENMGAQMLREVASK
ANDAAGDGTTTATVLAQAIVREGMTAVAAGMNPMDLKRGIDIAVGKVVENLKARSTPVAGSSEIAQVGIISANGDTEVGQ
KIAEAMEKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMTVELENPYILIHEKKLSSLQAMLPILEAV
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLGDIATLTAGEMISEDLGIKLESVTLAMLGQA
KKVTIDKDNTTIVDGAGSAEEIKARVEQIRAQIEVTTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDAL
HATRAAVEEGIVPGGGTALLYATKALEGLKGANDDQTKGIDIVRRAIQAPIRQIAANAGHDGAVVSGNLLRENDENQGFN
AATDTYENLKAAGVIDPTKVVRTALQDAASVSGLLITTEAAISEKPDDKPAMPPMGGGMGGMGGMDF

Sequences:

>Translated_547_residues
MAAKDVKFGRDARERILRGVDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELKDKFENMGAQMLREVASK
ANDAAGDGTTTATVLAQAIVREGMTAVAAGMNPMDLKRGIDIAVGKVVENLKARSTPVAGSSEIAQVGIISANGDTEVGQ
KIAEAMEKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMTVELENPYILIHEKKLSSLQAMLPILEAV
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLGDIATLTAGEMISEDLGIKLESVTLAMLGQA
KKVTIDKDNTTIVDGAGSAEEIKARVEQIRAQIEVTTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDAL
HATRAAVEEGIVPGGGTALLYATKALEGLKGANDDQTKGIDIVRRAIQAPIRQIAANAGHDGAVVSGNLLRENDENQGFN
AATDTYENLKAAGVIDPTKVVRTALQDAASVSGLLITTEAAISEKPDDKPAMPPMGGGMGGMGGMDF
>Mature_546_residues
AAKDVKFGRDARERILRGVDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELKDKFENMGAQMLREVASKA
NDAAGDGTTTATVLAQAIVREGMTAVAAGMNPMDLKRGIDIAVGKVVENLKARSTPVAGSSEIAQVGIISANGDTEVGQK
IAEAMEKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMTVELENPYILIHEKKLSSLQAMLPILEAVV
QSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLGDIATLTAGEMISEDLGIKLESVTLAMLGQAK
KVTIDKDNTTIVDGAGSAEEIKARVEQIRAQIEVTTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALH
ATRAAVEEGIVPGGGTALLYATKALEGLKGANDDQTKGIDIVRRAIQAPIRQIAANAGHDGAVVSGNLLRENDENQGFNA
ATDTYENLKAAGVIDPTKVVRTALQDAASVSGLLITTEAAISEKPDDKPAMPPMGGGMGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=52.0833333333333, Blast_Score=545, Evalue=1e-155,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=52.0833333333333, Blast_Score=545, Evalue=1e-155,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=69.1148775894539, Blast_Score=718, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=53.030303030303, Blast_Score=540, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=52.3809523809524, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17564182, Length=557, Percent_Identity=22.262118491921, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=58.0152671755725, Blast_Score=600, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6322524, Length=192, Percent_Identity=24.4791666666667, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24641193, Length=529, Percent_Identity=55.5765595463138, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24641191, Length=529, Percent_Identity=55.5765595463138, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52.5714285714286, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52.5714285714286, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52.5714285714286, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI17864606, Length=550, Percent_Identity=46.3636363636364, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=36.3295880149813, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=36.3295880149813, Blast_Score=321, Evalue=8e-88,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_NOVAD (Q2G2Z4)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495318.1
- HSSP:   P0A6F5
- ProteinModelPortal:   Q2G2Z4
- SMR:   Q2G2Z4
- STRING:   Q2G2Z4
- GeneID:   3916038
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0035
- NMPDR:   fig|48935.1.peg.3817
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NKPETAT
- PhylomeDB:   Q2G2Z4
- ProtClustDB:   PRK12849
- BioCyc:   NARO279238:SARO_0035-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57659; Mature: 57528

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDVKFGRDARERILRGVDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
ELKDKFENMGAQMLREVASKANDAAGDGTTTATVLAQAIVREGMTAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCC
DIAVGKVVENLKARSTPVAGSSEIAQVGIISANGDTEVGQKIAEAMEKVGKEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
KGLEFELDVVEGMQFDRGYLSPYFITNPEKMTVELENPYILIHEKKLSSLQAMLPILEAV
CCCEEEEEHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLGDIATLTAGEM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ISEDLGIKLESVTLAMLGQAKKVTIDKDNTTIVDGAGSAEEIKARVEQIRAQIEVTTSDY
HHHHHCCEEHHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHEEEECCCC
DREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALHATRAAVEEGIVPGGGTALL
CHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YATKALEGLKGANDDQTKGIDIVRRAIQAPIRQIAANAGHDGAVVSGNLLRENDENQGFN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
AATDTYENLKAAGVIDPTKVVRTALQDAASVSGLLITTEAAISEKPDDKPAMPPMGGGMG
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC
>Mature Secondary Structure 
AAKDVKFGRDARERILRGVDILADAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHH
ELKDKFENMGAQMLREVASKANDAAGDGTTTATVLAQAIVREGMTAVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCC
DIAVGKVVENLKARSTPVAGSSEIAQVGIISANGDTEVGQKIAEAMEKVGKEGVITVEEA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
KGLEFELDVVEGMQFDRGYLSPYFITNPEKMTVELENPYILIHEKKLSSLQAMLPILEAV
CCCEEEEEHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGRPLLIIAEDIEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLGDIATLTAGEM
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ISEDLGIKLESVTLAMLGQAKKVTIDKDNTTIVDGAGSAEEIKARVEQIRAQIEVTTSDY
HHHHHCCEEHHHHHHHHCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHEEEECCCC
DREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALHATRAAVEEGIVPGGGTALL
CHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
YATKALEGLKGANDDQTKGIDIVRRAIQAPIRQIAANAGHDGAVVSGNLLRENDENQGFN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCC
AATDTYENLKAAGVIDPTKVVRTALQDAASVSGLLITTEAAISEKPDDKPAMPPMGGGMG
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA