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The map label for this gene is tfdFI [H]

Identifier: 86751797

GI number: 86751797

Start: 5317897

End: 5319039

Strand: Direct

Name: tfdFI [H]

Synonym: RPB_4699

Alternate gene names: 86751797

Gene position: 5317897-5319039 (Clockwise)

Preceding gene: 86751787

Following gene: 86751798

Centisome position: 99.74

GC content: 70.6

Gene sequence:

>1143_bases
GTGCAAACGGGACGCGTCGTGTTCGGCGCCATGGAGGAAGTCGTTTACGGGCAGCCGGCGGCGGCAGCGATCGCCGCCCA
GATCGACCGGATCGGCGCGCAGCGCGCGCTGCTGATGGTCTCCGGCACCCTCAACCGCACCACCGACGAGATCGCCAAGA
TCCGCGACGCAATCGGACCGCGCTGCGCCGGGGTGTTCGACGCGATGCCGGCGCATTCGCCGCGCTCGGCGGTGATCGCC
GCGGCGAATCAGGCCCGCGCCGTCGGCGCCGACCTGCTCGTCACGGTCGGCGGCGGCTCGATCACCGACGGCGCCAAGGC
GGTGCAGATCTGCCTCGCCAACGACATCGGCACCGTGGCGGAGATGGACCGCGTCGCCAACCGGCCGTCGCCGGTGGCGC
CCATCGTGCGGCAGATCAGCGTGCCGACCACGATCTCCGGCGGCGAGTTCAGCGCGATGGCCGGAGTCACCAACGAGGCG
ACGCGGGTCAAGGAGAAATTCGCGCATCCGCTGGTGATGCCGCGCGCCGCGATCCTCGATCCGGCGATCACCGTGCACAC
GCCCGAATGGCTGTTCCTGTCGACCGGAATCCGTGCGGTCGATCACTGCGTCGAGGCGATCTGTTCGGGCGAGGCGCATC
CTTACGGCGATGCGCAGGCGCTGCATGGGCTGGCGATGCTGGCCAGCGCACTACCGCGGGTGAAAGCCGACGCGACCGAT
CTCGACGCCCGGCTCGACTGCCAGATCGGCACTTGGCTGTCGATGGGCGCGCTGTCGTCCGGCGTGCCGATGGGCGCCAG
CCACGGCATCGGCTACGTGCTCGGCGCGGTGTTCGACGTGCCGCACGGCCACACCTCCTGCGTGATGCTGCCGGCGGTGA
TGCGCTGGAACAAGTCGGTCAACCGCGAGCGCCAGGCGCTGGTCGCGGCCGCGATGGGCCACCCCGGCAAGGACGCTGGC
GACGTGCTCGACGCGCTGATCCGCGTGCTCGGGATGCCGCGCAGCCTCCGCGAGGTGAATGTCGGCCCCGAACATTTCGA
GCGCATCGCCGATCAGGCGATGGGCACGCCATGGGTGCCGCGCAACCCCCGCAAGATCGACGGTCCCGCGCAGGTGCGCG
AGATCCTGGAGCTGGCGGCGTAA

Upstream 100 bases:

>100_bases
GCGGCCCGACGCGCGACGATTTTTCTTGTTCCCGATCGCCGCGCGGCTGCTAGGATCATCGCCAAGGCCTCGCAAAAGCG
GCCAAGGGGAGTGAAGAAAC

Downstream 100 bases:

>100_bases
GCGACGCGCGCATGCTCCCCTCGGGACGAACAACGATGACTCCGTCATTGCGAGCGCAGCGAAGCAATCCAGAAGCTCAG
TGCAACGCGCCTCTGGATTG

Product: Iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: Maleylacetate reductase I [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MQTGRVVFGAMEEVVYGQPAAAAIAAQIDRIGAQRALLMVSGTLNRTTDEIAKIRDAIGPRCAGVFDAMPAHSPRSAVIA
AANQARAVGADLLVTVGGGSITDGAKAVQICLANDIGTVAEMDRVANRPSPVAPIVRQISVPTTISGGEFSAMAGVTNEA
TRVKEKFAHPLVMPRAAILDPAITVHTPEWLFLSTGIRAVDHCVEAICSGEAHPYGDAQALHGLAMLASALPRVKADATD
LDARLDCQIGTWLSMGALSSGVPMGASHGIGYVLGAVFDVPHGHTSCVMLPAVMRWNKSVNRERQALVAAAMGHPGKDAG
DVLDALIRVLGMPRSLREVNVGPEHFERIADQAMGTPWVPRNPRKIDGPAQVREILELAA

Sequences:

>Translated_380_residues
MQTGRVVFGAMEEVVYGQPAAAAIAAQIDRIGAQRALLMVSGTLNRTTDEIAKIRDAIGPRCAGVFDAMPAHSPRSAVIA
AANQARAVGADLLVTVGGGSITDGAKAVQICLANDIGTVAEMDRVANRPSPVAPIVRQISVPTTISGGEFSAMAGVTNEA
TRVKEKFAHPLVMPRAAILDPAITVHTPEWLFLSTGIRAVDHCVEAICSGEAHPYGDAQALHGLAMLASALPRVKADATD
LDARLDCQIGTWLSMGALSSGVPMGASHGIGYVLGAVFDVPHGHTSCVMLPAVMRWNKSVNRERQALVAAAMGHPGKDAG
DVLDALIRVLGMPRSLREVNVGPEHFERIADQAMGTPWVPRNPRKIDGPAQVREILELAA
>Mature_380_residues
MQTGRVVFGAMEEVVYGQPAAAAIAAQIDRIGAQRALLMVSGTLNRTTDEIAKIRDAIGPRCAGVFDAMPAHSPRSAVIA
AANQARAVGADLLVTVGGGSITDGAKAVQICLANDIGTVAEMDRVANRPSPVAPIVRQISVPTTISGGEFSAMAGVTNEA
TRVKEKFAHPLVMPRAAILDPAITVHTPEWLFLSTGIRAVDHCVEAICSGEAHPYGDAQALHGLAMLASALPRVKADATD
LDARLDCQIGTWLSMGALSSGVPMGASHGIGYVLGAVFDVPHGHTSCVMLPAVMRWNKSVNRERQALVAAAMGHPGKDAG
DVLDALIRVLGMPRSLREVNVGPEHFERIADQAMGTPWVPRNPRKIDGPAQVREILELAA

Specific function: May Act On The Acetaldehyde Produced From The Degradation Of Ethanolamine. [C]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082107, Length=307, Percent_Identity=31.9218241042345, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789163, Length=395, Percent_Identity=27.8481012658228, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994951, Length=311, Percent_Identity=28.2958199356913, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787493, Length=373, Percent_Identity=26.8096514745308, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789386, Length=306, Percent_Identity=23.8562091503268, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321181, Length=305, Percent_Identity=27.5409836065574, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.3.1.32 [H]

Molecular weight: Translated: 39817; Mature: 39817

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTGRVVFGAMEEVVYGQPAAAAIAAQIDRIGAQRALLMVSGTLNRTTDEIAKIRDAIGP
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCC
RCAGVFDAMPAHSPRSAVIAAANQARAVGADLLVTVGGGSITDGAKAVQICLANDIGTVA
HHHHHHHCCCCCCCCHHEEEECHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHH
EMDRVANRPSPVAPIVRQISVPTTISGGEFSAMAGVTNEATRVKEKFAHPLVMPRAAILD
HHHHHHCCCCCHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHCCCHHCCHHHHHC
PAITVHTPEWLFLSTGIRAVDHCVEAICSGEAHPYGDAQALHGLAMLASALPRVKADATD
CCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LDARLDCQIGTWLSMGALSSGVPMGASHGIGYVLGAVFDVPHGHTSCVMLPAVMRWNKSV
CCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
NRERQALVAAAMGHPGKDAGDVLDALIRVLGMPRSLREVNVGPEHFERIADQAMGTPWVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCC
RNPRKIDGPAQVREILELAA
CCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQTGRVVFGAMEEVVYGQPAAAAIAAQIDRIGAQRALLMVSGTLNRTTDEIAKIRDAIGP
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHCCC
RCAGVFDAMPAHSPRSAVIAAANQARAVGADLLVTVGGGSITDGAKAVQICLANDIGTVA
HHHHHHHCCCCCCCCHHEEEECHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHH
EMDRVANRPSPVAPIVRQISVPTTISGGEFSAMAGVTNEATRVKEKFAHPLVMPRAAILD
HHHHHHCCCCCHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHCCCHHCCHHHHHC
PAITVHTPEWLFLSTGIRAVDHCVEAICSGEAHPYGDAQALHGLAMLASALPRVKADATD
CCEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
LDARLDCQIGTWLSMGALSSGVPMGASHGIGYVLGAVFDVPHGHTSCVMLPAVMRWNKSV
CCCEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
NRERQALVAAAMGHPGKDAGDVLDALIRVLGMPRSLREVNVGPEHFERIADQAMGTPWVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCC
RNPRKIDGPAQVREILELAA
CCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]