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The map label for this gene is braE [H]

Identifier: 86751526

GI number: 86751526

Start: 5014043

End: 5014999

Strand: Direct

Name: braE [H]

Synonym: RPB_4425

Alternate gene names: 86751526

Gene position: 5014043-5014999 (Clockwise)

Preceding gene: 86751525

Following gene: 86751527

Centisome position: 94.04

GC content: 65.41

Gene sequence:

>957_bases
ATGACCGAGCCCCGCTGGACTCCCTACGCCGTTCTCGTCGCGATCGCCCTGCTGGCGGCGCTGCCTTTGATGACCGGTTC
CAACTACATCGTCGGCATCGCGATTTCGGGGCTGATCTTCCTCACCGCCGCGGCGGCGCTCAACCTCGTCTACGGCTATG
CCGGCCAACTCTCCTTCGCACAGCTCGGCTTCTGGGGCGTCGGCGGCTATTGCGCGGCGATCGCCGTCGTCGATCTCGGG
CAGAGCTTCTGGTTCGGCATCGCCTTTGCCGGGGTCGTCAACCTGCTGCTCTCCGTCGTCATCGGCATCGTCATCACACG
CACCACGCGCCACGCCTTCGTGGTGGTGACCCTGACCTTCGCGCTGCTCGCCGGCCTGATCGCACGCGACTGGGTGGGCG
TGACCCGCGGCCCGCTCGGCATCCCCGGCCTGCCGCCGCCGAGCCTGATGGGATTCGCCTTCGACACCGGCGCCCGGTTC
TACTACATCGCGCTGGTCTTCGCGCTGATCGCGCTGGCTTTCCTGTACAGCCTGAAGACCTCGCGCATCGGCCTGATCCT
GAAGGCTATCAAACAGAACGATCCGCTGGTGGAATCCCAGGGCATCGCGCCCGGCCCCTACAAGCTCGCCGCCTTCGTGA
TCGCCTCGGTCATCACCGGCATGGCCGGCGGCATCTATGCGTTCAACCTCAAGGTGATCGATCCGAGCTTCCTCGATTTC
TACTACATGCAGACCTTCCTGATCATCGTCATCATCGGCGGAGCCGGCAGCTTCTGGGCCGTCGTGCTGGCGGGCGCCGC
GATGGTCGTGCTGCCTGAAGCCCTGCGTTTTTCCAACGAACTGCGCATGGTGATCTATGGCGTGGTGCTGGTCGCGGTGA
TGATGTTCATGCCGCGCGGCGTGGCCGGCTGGCTCGAGGATCGGCGGATCGCCGCCTTGCGGAAGGCGCTGCGATGA

Upstream 100 bases:

>100_bases
TCAGCATCGTCTGGCAAGAAGGCGCGATCTTCGTGATCATGATCGTCGCGGTGTTGCTCCGTCCGGACGGACTTTTCAAG
CGCGGGAGCATGCGCGTCGG

Downstream 100 bases:

>100_bases
GCGCCATCCTGAACGTCGCGGATGTCAGCAAGGCCTTCAGCGGCGTGCATGCGGTGCAGGGCACGTCGTTCGAGGTGGAA
GCGGGCAGTATCACCGGCCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTEPRWTPYAVLVAIALLAALPLMTGSNYIVGIAISGLIFLTAAAALNLVYGYAGQLSFAQLGFWGVGGYCAAIAVVDLG
QSFWFGIAFAGVVNLLLSVVIGIVITRTTRHAFVVVTLTFALLAGLIARDWVGVTRGPLGIPGLPPPSLMGFAFDTGARF
YYIALVFALIALAFLYSLKTSRIGLILKAIKQNDPLVESQGIAPGPYKLAAFVIASVITGMAGGIYAFNLKVIDPSFLDF
YYMQTFLIIVIIGGAGSFWAVVLAGAAMVVLPEALRFSNELRMVIYGVVLVAVMMFMPRGVAGWLEDRRIAALRKALR

Sequences:

>Translated_318_residues
MTEPRWTPYAVLVAIALLAALPLMTGSNYIVGIAISGLIFLTAAAALNLVYGYAGQLSFAQLGFWGVGGYCAAIAVVDLG
QSFWFGIAFAGVVNLLLSVVIGIVITRTTRHAFVVVTLTFALLAGLIARDWVGVTRGPLGIPGLPPPSLMGFAFDTGARF
YYIALVFALIALAFLYSLKTSRIGLILKAIKQNDPLVESQGIAPGPYKLAAFVIASVITGMAGGIYAFNLKVIDPSFLDF
YYMQTFLIIVIIGGAGSFWAVVLAGAAMVVLPEALRFSNELRMVIYGVVLVAVMMFMPRGVAGWLEDRRIAALRKALR
>Mature_317_residues
TEPRWTPYAVLVAIALLAALPLMTGSNYIVGIAISGLIFLTAAAALNLVYGYAGQLSFAQLGFWGVGGYCAAIAVVDLGQ
SFWFGIAFAGVVNLLLSVVIGIVITRTTRHAFVVVTLTFALLAGLIARDWVGVTRGPLGIPGLPPPSLMGFAFDTGARFY
YIALVFALIALAFLYSLKTSRIGLILKAIKQNDPLVESQGIAPGPYKLAAFVIASVITGMAGGIYAFNLKVIDPSFLDFY
YMQTFLIIVIIGGAGSFWAVVLAGAAMVVLPEALRFSNELRMVIYGVVLVAVMMFMPRGVAGWLEDRRIAALRKALR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=303, Percent_Identity=28.0528052805281, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33941; Mature: 33810

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPRWTPYAVLVAIALLAALPLMTGSNYIVGIAISGLIFLTAAAALNLVYGYAGQLSFA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QLGFWGVGGYCAAIAVVDLGQSFWFGIAFAGVVNLLLSVVIGIVITRTTRHAFVVVTLTF
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALLAGLIARDWVGVTRGPLGIPGLPPPSLMGFAFDTGARFYYIALVFALIALAFLYSLKT
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
SRIGLILKAIKQNDPLVESQGIAPGPYKLAAFVIASVITGMAGGIYAFNLKVIDPSFLDF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHH
YYMQTFLIIVIIGGAGSFWAVVLAGAAMVVLPEALRFSNELRMVIYGVVLVAVMMFMPRG
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VAGWLEDRRIAALRKALR
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEPRWTPYAVLVAIALLAALPLMTGSNYIVGIAISGLIFLTAAAALNLVYGYAGQLSFA
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QLGFWGVGGYCAAIAVVDLGQSFWFGIAFAGVVNLLLSVVIGIVITRTTRHAFVVVTLTF
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALLAGLIARDWVGVTRGPLGIPGLPPPSLMGFAFDTGARFYYIALVFALIALAFLYSLKT
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
SRIGLILKAIKQNDPLVESQGIAPGPYKLAAFVIASVITGMAGGIYAFNLKVIDPSFLDF
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHH
YYMQTFLIIVIIGGAGSFWAVVLAGAAMVVLPEALRFSNELRMVIYGVVLVAVMMFMPRG
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VAGWLEDRRIAALRKALR
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]