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The map label for this gene is ruvC [H]

Identifier: 86751375

GI number: 86751375

Start: 4855962

End: 4856558

Strand: Reverse

Name: ruvC [H]

Synonym: RPB_4272

Alternate gene names: 86751375

Gene position: 4856558-4855962 (Counterclockwise)

Preceding gene: 86751376

Following gene: 162138516

Centisome position: 91.09

GC content: 68.17

Gene sequence:

>597_bases
GTGGGTGGGGATGATCCCGTCGTCCAAACCGTTTCTGTTTCGTTCACGTCTATCTGGCGTTATCACTGCGCCATGAGCCT
TCCGCCGATTCGCACCGCTGTCCGCATCCTGGGGATCGACCCCGGCCTTCGCCGCACCGGCTGGGGCGTGGTCGAGAGCG
ACGGCAATCGGCTGGTGTTCGTGGCCTGCGGCTCGGTCGAACCGAAGGACACGTTGACGCTGGCGGAGCGGCTGCTGGCG
ATCCATCATGGGCTCGAGAAGGTGCTGGCCGAGTTCGCGCCGGCGGAGGCCGCGGTCGAGCAGACCTTCGTCAACAAGGA
CGGCGCCGCCACGCTGAAGCTCGGCCAGGCGCGGGGCGTGGCGATGCTGGCGCCGGCGATGCACGGCATTCTGGTCGCCG
AATACGCGCCCAACCAGGTCAAGAAGACGGTGGTCGGCGCCGGCCATGCCGACAAGGTCCAGATCCAGATGATGCTGAAG
ATATTGCTGCCCAAGGCCGATCCGCGCACCGCGGACGCCGCCGACGCCCTCGCCATCGCCATCACCCACGCCCACCACCG
CGTCGCCGCGCAGCGGCTGAGGGCGGTGGGGGGATGA

Upstream 100 bases:

>100_bases
GCGACCCAGTATCCCAGAGCGTTCATGGTGGGGCGTAGCCGCCGCTGCGGCCAAACGTCGGCGTTCTGGGATACTGGATC
CCCGCATTCGCGGGGATGAC

Downstream 100 bases:

>100_bases
GCGTGTTCATCCGCGGAACCAGCCCCGCGCACAGCGCGCTCCCTCTCCCGCTTGCGGGAGAGGGCTGGGGTGAGGGCGAC
GCGGGCACAACTTCTCCCTC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MGGDDPVVQTVSVSFTSIWRYHCAMSLPPIRTAVRILGIDPGLRRTGWGVVESDGNRLVFVACGSVEPKDTLTLAERLLA
IHHGLEKVLAEFAPAEAAVEQTFVNKDGAATLKLGQARGVAMLAPAMHGILVAEYAPNQVKKTVVGAGHADKVQIQMMLK
ILLPKADPRTADAADALAIAITHAHHRVAAQRLRAVGG

Sequences:

>Translated_198_residues
MGGDDPVVQTVSVSFTSIWRYHCAMSLPPIRTAVRILGIDPGLRRTGWGVVESDGNRLVFVACGSVEPKDTLTLAERLLA
IHHGLEKVLAEFAPAEAAVEQTFVNKDGAATLKLGQARGVAMLAPAMHGILVAEYAPNQVKKTVVGAGHADKVQIQMMLK
ILLPKADPRTADAADALAIAITHAHHRVAAQRLRAVGG
>Mature_197_residues
GGDDPVVQTVSVSFTSIWRYHCAMSLPPIRTAVRILGIDPGLRRTGWGVVESDGNRLVFVACGSVEPKDTLTLAERLLAI
HHGLEKVLAEFAPAEAAVEQTFVNKDGAATLKLGQARGVAMLAPAMHGILVAEYAPNQVKKTVVGAGHADKVQIQMMLKI
LLPKADPRTADAADALAIAITHAHHRVAAQRLRAVGG

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=151, Percent_Identity=45.0331125827815, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 20990; Mature: 20859

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDDPVVQTVSVSFTSIWRYHCAMSLPPIRTAVRILGIDPGLRRTGWGVVESDGNRLVF
CCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCCEEEECCCCEEEE
VACGSVEPKDTLTLAERLLAIHHGLEKVLAEFAPAEAAVEQTFVNKDGAATLKLGQARGV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCC
AMLAPAMHGILVAEYAPNQVKKTVVGAGHADKVQIQMMLKILLPKADPRTADAADALAIA
HHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ITHAHHRVAAQRLRAVGG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGDDPVVQTVSVSFTSIWRYHCAMSLPPIRTAVRILGIDPGLRRTGWGVVESDGNRLVF
CCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCCEEEECCCCEEEE
VACGSVEPKDTLTLAERLLAIHHGLEKVLAEFAPAEAAVEQTFVNKDGAATLKLGQARGV
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCC
AMLAPAMHGILVAEYAPNQVKKTVVGAGHADKVQIQMMLKILLPKADPRTADAADALAIA
HHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ITHAHHRVAAQRLRAVGG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA