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The map label for this gene is ligE [H]

Identifier: 86751250

GI number: 86751250

Start: 4717928

End: 4718623

Strand: Reverse

Name: ligE [H]

Synonym: RPB_4143

Alternate gene names: 86751250

Gene position: 4718623-4717928 (Counterclockwise)

Preceding gene: 86751251

Following gene: 86751248

Centisome position: 88.5

GC content: 67.96

Gene sequence:

>696_bases
ATGACCCTCAAACTCTTCGAACTGGTCGGCACCGACGCGGCGCGGCCGTTTTCGCCGTTCTGCTGGCGGATCCGGATGGC
GCTGGCGCACAAGGGGCTCGCCGCCGAGACCATTCCATGGCGCTTCACCGAGAAATCGGCGATCACGCCGTATAGTTCCG
AGAAGGTGCCGGTGCTGCTCGACGGCGACACGGCCGTGTGCGACTCCTGGGCGATCGCGACCTATCTCGAAGACGCCTAT
CCCGACCGGCCGTCGTTGTTCGGCGGCGACGGCGGCCGGGCGATGGCGCGGATGATCAACGCCTGGGGCGACGTCACCCT
GGTCGCCGGCCTGGCGCCGCTGATCGTCGCCGATATCGTCAAGCTGCTGGGGCCCGACGACACCGCGTATTTCCGCAAGA
GCCGCGAGGCGCGCTTCGGCAGGACGCTGGAGGAGGTCGCGAGCACGCGCGACAGCGCGGTCGAAGGTTTCCGCAAGTCG
CTCGACCCGCTGCGACTGACTTTGAAGGCGCAGCCCTTCCTCGGCGGCGCGGCGCCGAACTACGCGGATTACATCGCGTT
CGGCGGCTTCCAATGGGCCCGCGCCGTCAGTGACTTCGCGCTGCTGAAGCCCGACGATCCGGTCCACGCCTGGCGCGGGA
AACTGCTCGACGCCTTTGGCGGCATGGCGCGAAACTCGGGTGGCTATCCGGTGTGA

Upstream 100 bases:

>100_bases
AAGGCCAAGGAGATCAACCCCGATTTCGGCCAGCCGACGATCCGCACCGAGGAAGAGAAGAAACTGCTGTTCGGCCAGAC
CGCGGCGGTGGTGAAGTAAT

Downstream 100 bases:

>100_bases
GCATCGGACGGTGACGCCGCATAGCCGGCGCCGCAGTGGCACAAGCGGCCGTTGAGCCAGCACCACGTCATCCTGAGGTG
CGAGCGCAGCGAGCCTCGAA

Product: glutathione S-transferase-like protein

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTLKLFELVGTDAARPFSPFCWRIRMALAHKGLAAETIPWRFTEKSAITPYSSEKVPVLLDGDTAVCDSWAIATYLEDAY
PDRPSLFGGDGGRAMARMINAWGDVTLVAGLAPLIVADIVKLLGPDDTAYFRKSREARFGRTLEEVASTRDSAVEGFRKS
LDPLRLTLKAQPFLGGAAPNYADYIAFGGFQWARAVSDFALLKPDDPVHAWRGKLLDAFGGMARNSGGYPV

Sequences:

>Translated_231_residues
MTLKLFELVGTDAARPFSPFCWRIRMALAHKGLAAETIPWRFTEKSAITPYSSEKVPVLLDGDTAVCDSWAIATYLEDAY
PDRPSLFGGDGGRAMARMINAWGDVTLVAGLAPLIVADIVKLLGPDDTAYFRKSREARFGRTLEEVASTRDSAVEGFRKS
LDPLRLTLKAQPFLGGAAPNYADYIAFGGFQWARAVSDFALLKPDDPVHAWRGKLLDAFGGMARNSGGYPV
>Mature_230_residues
TLKLFELVGTDAARPFSPFCWRIRMALAHKGLAAETIPWRFTEKSAITPYSSEKVPVLLDGDTAVCDSWAIATYLEDAYP
DRPSLFGGDGGRAMARMINAWGDVTLVAGLAPLIVADIVKLLGPDDTAYFRKSREARFGRTLEEVASTRDSAVEGFRKSL
DPLRLTLKAQPFLGGAAPNYADYIAFGGFQWARAVSDFALLKPDDPVHAWRGKLLDAFGGMARNSGGYPV

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25194; Mature: 25063

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKLFELVGTDAARPFSPFCWRIRMALAHKGLAAETIPWRFTEKSAITPYSSEKVPVLL
CCEEHEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEE
DGDTAVCDSWAIATYLEDAYPDRPSLFGGDGGRAMARMINAWGDVTLVAGLAPLIVADIV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KLLGPDDTAYFRKSREARFGRTLEEVASTRDSAVEGFRKSLDPLRLTLKAQPFLGGAAPN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
YADYIAFGGFQWARAVSDFALLKPDDPVHAWRGKLLDAFGGMARNSGGYPV
HHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
TLKLFELVGTDAARPFSPFCWRIRMALAHKGLAAETIPWRFTEKSAITPYSSEKVPVLL
CEEHEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEE
DGDTAVCDSWAIATYLEDAYPDRPSLFGGDGGRAMARMINAWGDVTLVAGLAPLIVADIV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KLLGPDDTAYFRKSREARFGRTLEEVASTRDSAVEGFRKSLDPLRLTLKAQPFLGGAAPN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
YADYIAFGGFQWARAVSDFALLKPDDPVHAWRGKLLDAFGGMARNSGGYPV
HHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]