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The map label for this gene is rutD [H]

Identifier: 86751248

GI number: 86751248

Start: 4715664

End: 4716365

Strand: Reverse

Name: rutD [H]

Synonym: RPB_4141

Alternate gene names: 86751248

Gene position: 4716365-4715664 (Counterclockwise)

Preceding gene: 86751250

Following gene: 86751247

Centisome position: 88.46

GC content: 69.23

Gene sequence:

>702_bases
ATGGACACTTCGTTGCCGATCCTGTTGATCCCGGGGCTGGGCGGCTCGCCGCGGATCTACGCCCCGGTGCTGCCGTCGCT
GTGGACATTCGGCCCGGTGACGGTCGCCAACCACATTCGCGATGACAGCATGGCGGCGATCGCCGGGCGAATTCTGGCCG
AAGCTCCGCCGCGCTTCGCGCTCGCCGGACATTCGATGGGCGGCTACATCGCCTTCGAGATCCTGCGCCAGGCGCCCGAG
CGGGTGGCGAGGCTGGCGCTGATCAATACCCAGGCGCGTCCGGACAGCGACGAGGCCAAGGAGCGCCGCACCCGGCAGAT
CGCGCAGGCCGAAGCCGGCCAGCTTCATGCGGTGCTCGACGTCCTTTATCCTGGCTTCGTGCATCCGTCGCGCTGTGAAG
ACGCCAGGCTGCAGCGCGTCATCCACGACATGGGCGACGATGTCGGTGCAGACGGCTTCGTCCGCCAGCAGCGCGCGATC
ATCGCCCGCGCCGACTCCCGGCCGACGTTGGCGACCATCCGCTGCCCGACGCTGGTGATCTCCGGCGACACCGACAACAC
CATTCCCAACAGCCTGTCGCAGGAGATGGCCGAGGGCATCGCCGGCGCCAAGCTGGAGATCATCCCGGACTGCGGCCACC
TCCCGCAGATCGAGCAGCCGCAGGCGACCGCGGCGGCGCTGGCCGGCTGGCTGCGGAACTGA

Upstream 100 bases:

>100_bases
GGCATTTCGTTCGGTTTTCGACGAGATTTGACAGGGATGTTGCCCTGTCTAGGCTCCCGCCCGGGCGCGCGGGACGGATT
CGTGCGCCGAGGGAGGTTTC

Downstream 100 bases:

>100_bases
GTTGTTTCGGCCGGCCGAACCGCCTACATCAGGCGCAACAGACGCCGTTTCCTCGTCATTGCGAGGAGCGCAGCGACGAA
GCAATCCAGGGGCGCGGTGC

Product: Alpha/beta hydrolase protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MDTSLPILLIPGLGGSPRIYAPVLPSLWTFGPVTVANHIRDDSMAAIAGRILAEAPPRFALAGHSMGGYIAFEILRQAPE
RVARLALINTQARPDSDEAKERRTRQIAQAEAGQLHAVLDVLYPGFVHPSRCEDARLQRVIHDMGDDVGADGFVRQQRAI
IARADSRPTLATIRCPTLVISGDTDNTIPNSLSQEMAEGIAGAKLEIIPDCGHLPQIEQPQATAAALAGWLRN

Sequences:

>Translated_233_residues
MDTSLPILLIPGLGGSPRIYAPVLPSLWTFGPVTVANHIRDDSMAAIAGRILAEAPPRFALAGHSMGGYIAFEILRQAPE
RVARLALINTQARPDSDEAKERRTRQIAQAEAGQLHAVLDVLYPGFVHPSRCEDARLQRVIHDMGDDVGADGFVRQQRAI
IARADSRPTLATIRCPTLVISGDTDNTIPNSLSQEMAEGIAGAKLEIIPDCGHLPQIEQPQATAAALAGWLRN
>Mature_233_residues
MDTSLPILLIPGLGGSPRIYAPVLPSLWTFGPVTVANHIRDDSMAAIAGRILAEAPPRFALAGHSMGGYIAFEILRQAPE
RVARLALINTQARPDSDEAKERRTRQIAQAEAGQLHAVLDVLYPGFVHPSRCEDARLQRVIHDMGDDVGADGFVRQQRAI
IARADSRPTLATIRCPTLVISGDTDNTIPNSLSQEMAEGIAGAKLEIIPDCGHLPQIEQPQATAAALAGWLRN

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 25073; Mature: 25073

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSLPILLIPGLGGSPRIYAPVLPSLWTFGPVTVANHIRDDSMAAIAGRILAEAPPRFA
CCCCCCEEEECCCCCCCEECCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEE
LAGHSMGGYIAFEILRQAPERVARLALINTQARPDSDEAKERRTRQIAQAEAGQLHAVLD
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLYPGFVHPSRCEDARLQRVIHDMGDDVGADGFVRQQRAIIARADSRPTLATIRCPTLVI
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEEEECCEEEE
SGDTDNTIPNSLSQEMAEGIAGAKLEIIPDCGHLPQIEQPQATAAALAGWLRN
ECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDTSLPILLIPGLGGSPRIYAPVLPSLWTFGPVTVANHIRDDSMAAIAGRILAEAPPRFA
CCCCCCEEEECCCCCCCEECCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEE
LAGHSMGGYIAFEILRQAPERVARLALINTQARPDSDEAKERRTRQIAQAEAGQLHAVLD
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VLYPGFVHPSRCEDARLQRVIHDMGDDVGADGFVRQQRAIIARADSRPTLATIRCPTLVI
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCEEEEECCEEEE
SGDTDNTIPNSLSQEMAEGIAGAKLEIIPDCGHLPQIEQPQATAAALAGWLRN
ECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA