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The map label for this gene is yfcG [H]

Identifier: 86751251

GI number: 86751251

Start: 4718625

End: 4719332

Strand: Reverse

Name: yfcG [H]

Synonym: RPB_4144

Alternate gene names: 86751251

Gene position: 4719332-4718625 (Counterclockwise)

Preceding gene: 86751252

Following gene: 86751250

Centisome position: 88.52

GC content: 62.29

Gene sequence:

>708_bases
ATGATCGATCTGTATTACTGGACCACGCCGAACGGCCACAAGATCACGATGTTCCTGGAAGAAGCGGGGCTGCCGTACGG
CATCGTCCCGGTGAATATCGGCAAGGGCGATCAGTTCAAGCCGGACTTCCTCGCGATCGCGCCGAACAACCGGATGCCGG
CGATCGTCGATCATCAGCCGGCCGGCGGCGGCACGCCGCTGTCGGTGTTCGAATCCGGTGCGATCCTGCTGTATCTCGCC
GACAAGACCGGGCAGTTTCTCTCAAAGGATCTGTACGCCCGCAACGACGCGCTGCAATGGCTGTTCTGGCAGATGGGCGG
GCTCGGGCCGATGGCCGGGCAGAACCACCATTTCAACAACTACGCGCCCGAGAAAATCCCCTACGCCATCAACCGCTACG
TCAACGAGACCAACCGGCTCTACGGCGTGCTCAACAAGCGCCTCGCCGACCGCGACTTCCTCGCCGGCGAGTATTCGATT
GCGGATATGGCGAGCTATCCGTGGATCGTGCCGCACAAGAACCAGAGCCAGAATATCGACGACTTCCCGCATCTGAAGCG
CTGGCTGGAGGCGATCGCCGCGCGCCCGGCCACGGTGCGCGCCTACGCCAAGGCCAAGGAGATCAACCCCGATTTCGGCC
AGCCGACGATCCGCACCGAGGAAGAGAAGAAACTGCTGTTCGGCCAGACCGCGGCGGTGGTGAAGTAA

Upstream 100 bases:

>100_bases
GGCGCGCCGGCGCCGCAGATATTGACTTTGGCCGGCAGTTGGCCAAAGTCCATGCATTCGCATCCAGTTTGGATGCCGCA
TCAGCCCGCGGGAAACGCCG

Downstream 100 bases:

>100_bases
TATGACCCTCAAACTCTTCGAACTGGTCGGCACCGACGCGGCGCGGCCGTTTTCGCCGTTCTGCTGGCGGATCCGGATGG
CGCTGGCGCACAAGGGGCTC

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIDLYYWTTPNGHKITMFLEEAGLPYGIVPVNIGKGDQFKPDFLAIAPNNRMPAIVDHQPAGGGTPLSVFESGAILLYLA
DKTGQFLSKDLYARNDALQWLFWQMGGLGPMAGQNHHFNNYAPEKIPYAINRYVNETNRLYGVLNKRLADRDFLAGEYSI
ADMASYPWIVPHKNQSQNIDDFPHLKRWLEAIAARPATVRAYAKAKEINPDFGQPTIRTEEEKKLLFGQTAAVVK

Sequences:

>Translated_235_residues
MIDLYYWTTPNGHKITMFLEEAGLPYGIVPVNIGKGDQFKPDFLAIAPNNRMPAIVDHQPAGGGTPLSVFESGAILLYLA
DKTGQFLSKDLYARNDALQWLFWQMGGLGPMAGQNHHFNNYAPEKIPYAINRYVNETNRLYGVLNKRLADRDFLAGEYSI
ADMASYPWIVPHKNQSQNIDDFPHLKRWLEAIAARPATVRAYAKAKEINPDFGQPTIRTEEEKKLLFGQTAAVVK
>Mature_235_residues
MIDLYYWTTPNGHKITMFLEEAGLPYGIVPVNIGKGDQFKPDFLAIAPNNRMPAIVDHQPAGGGTPLSVFESGAILLYLA
DKTGQFLSKDLYARNDALQWLFWQMGGLGPMAGQNHHFNNYAPEKIPYAINRYVNETNRLYGVLNKRLADRDFLAGEYSI
ADMASYPWIVPHKNQSQNIDDFPHLKRWLEAIAARPATVRAYAKAKEINPDFGQPTIRTEEEKKLLFGQTAAVVK

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=207, Percent_Identity=61.8357487922705, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI87082195, Length=214, Percent_Identity=42.5233644859813, Blast_Score=163, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=32.0512820512821, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17933730, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26455; Mature: 26455

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLYYWTTPNGHKITMFLEEAGLPYGIVPVNIGKGDQFKPDFLAIAPNNRMPAIVDHQP
CEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
AGGGTPLSVFESGAILLYLADKTGQFLSKDLYARNDALQWLFWQMGGLGPMAGQNHHFNN
CCCCCCHHEECCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
YAPEKIPYAINRYVNETNRLYGVLNKRLADRDFLAGEYSIADMASYPWIVPHKNQSQNID
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCC
DFPHLKRWLEAIAARPATVRAYAKAKEINPDFGQPTIRTEEEKKLLFGQTAAVVK
CCHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHCC
>Mature Secondary Structure
MIDLYYWTTPNGHKITMFLEEAGLPYGIVPVNIGKGDQFKPDFLAIAPNNRMPAIVDHQP
CEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
AGGGTPLSVFESGAILLYLADKTGQFLSKDLYARNDALQWLFWQMGGLGPMAGQNHHFNN
CCCCCCHHEECCCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCC
YAPEKIPYAINRYVNETNRLYGVLNKRLADRDFLAGEYSIADMASYPWIVPHKNQSQNID
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEECCCCCCCCCC
DFPHLKRWLEAIAARPATVRAYAKAKEINPDFGQPTIRTEEEKKLLFGQTAAVVK
CCHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]