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The map label for this gene is gatA [H]

Identifier: 86751165

GI number: 86751165

Start: 4627321

End: 4629120

Strand: Direct

Name: gatA [H]

Synonym: RPB_4057

Alternate gene names: 86751165

Gene position: 4627321-4629120 (Clockwise)

Preceding gene: 86751164

Following gene: 86751169

Centisome position: 86.79

GC content: 70.67

Gene sequence:

>1800_bases
GTGACTGAAACCATCGCCGACATTCTCGCCGCGCATCGCGCCGGCACCACGACGCCCGCGCAGACCATCGCGCGCTGCTA
TCAGCGCATCCGCGCTCATGCCGATCCCGCGCTGTTCATCACGCTGCGCGACGAGGCGGACGCCGTGGCCGAAGCCGTGG
CGCTCGCCGCCCGTGACCCGTCGCTGCCGCTCTACGGCGTGCCGGTCGCCGTCAAGGACAATATCGACGTCGCCGGCCTG
CCGACCACCGCGGCCTGTCCGGCTTTCGCGTATCAGCCGGCGCAGGATTCCACCGCGGTTGCGAAGCTGCGCGCCGCAGG
CGCGATCATCATCGGCAAGACCAATCTCGATCAGTTCGCCACCGGCCTGGTCGGCGTGCGCTCGCCCTACGGCATTCCGC
GCAACGCGATGCGCGCCGATCTGGTGCCGGGCGGCTCGAGTTCGGGTTCGGCGGTCGCGGTCGGCGCCGGCCTGGTGCCG
CTGTCCCTCGGCACCGACACCGCCGGCTCCGGCCGCGTCCCGGCGATGCTCAACAACATCGTCGGGCTGAAGCCGAGCCT
CGGCCTGATCTCGACCACCGGCCTGGTGCCGGCGTGCCGCACGCTGGATTGCATCTCGGTGTTCGCGCTGACCGTGGACG
ACGCGATGATCGCGCTGCGGGTGATGGGCACCCCCGACGCCACCGATCCGTATTCGCGCGCTCGGCCGATCGCGCCGATG
TCGGCGATGCCCGACAGGCCACGGCTCGGCGTACCGCGGCCCGATCAGTTGCAGTTTTTCGGCGATCAGCAATCCGAACA
GGCCTATGCCGACGCGCTGCAACGCTGGACGTCGCTCGGCGCCGAACTGATCGAGATCGATGTCGCGCCCTTATACGAGA
CCGCGCGGCTGCTCTATGACGGCCCGTGGGTCGCCGAGCGCTATCTCGCGATCCGCGAGCTGATCGACGCGACCCCCGAC
GCGCTGCATCCGGTGACGCGGCAGATCACGCTCGGCGGCAAGGCGATCAGCGCCGCCGACACATTCGCAGCGCTGTATCG
ACTGCAGGCGTTGCGCAAGCTCGCGGAGCCCGCCTTCGCGGCGATCGACGCGCTGGTGCTGCCGACGGCGCCGACCGCCT
ACACGGTCGAACAGGTGCTGGCCGATCCGGTCACGCTCAACAGCCGGCTCGGCACCTACACCAATTTCGTCAATCTGCTC
GACCTTTGCGGGCTCGCGATCCCGGCGTCGATCCGCGCCGACGGCATCCCGTTCGGCGTCACGCTGCTGGCGCCGGGCGG
CCGCGATGCCGAGCTCGCCAGCCTCGGCCGCATGTTCCACGCCGACACCGCGCTGCCGATGGGCGCGACGGGCGTCGCCC
TGCCGCCGCTGGCGGCGCTGGATGCGGACGCCGGCACGGACCACATTCAGATCGCGGTGGTCGGCGCGCATCTGTCCGGC
ATGGCGCTGAACGGCGAGCTGACGTCGCTGGACGGCCGACTGTTGCGCGCGACCGCGACGGCGCCGGACTACAAGCTCTA
TGCGCTGAACGGCACGGTGCCGCCGAAGCCCGGCATGCTGCGCGTCGCAGCAGGCGCAGGCGCGGCGATCGCGCTGGAAA
TCTGGTCGCTGTCGCCGGCCGCGTTCGGCCGCTTCGTCGCTGCGATCCCGCAGCCCTTGTCGATCGGCACCCTGAAGCTG
GCGGACGGCGCGCTGGTCAAAGGCTTCCTGGTCGAGCCCGCCGCGCTCGAGGTCGCCCGCGACATCACCCATTTCGGCGG
CTGGCGGGCCTATATGGCGGAGCTGGCGAAAGCGGGGTGA

Upstream 100 bases:

>100_bases
CGGCGACCGCAATGCCGCTGCGGCCGCGATGCGCGGCCATATCGCGCTGGTGCGCGACGAATATGAAATCTACGCCGTCT
CGGTGTGACTGGAGCTTTTC

Downstream 100 bases:

>100_bases
GGGCTGGCAAACCGAGCAGCGCGCAGCAACACCCAGCCGTCATCCCCGCGCAGGCGGGGATCCAGTATCCCGGAGCGCCG
GCGTTCAGCACGGTGCTAAC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTETIADILAAHRAGTTTPAQTIARCYQRIRAHADPALFITLRDEADAVAEAVALAARDPSLPLYGVPVAVKDNIDVAGL
PTTAACPAFAYQPAQDSTAVAKLRAAGAIIIGKTNLDQFATGLVGVRSPYGIPRNAMRADLVPGGSSSGSAVAVGAGLVP
LSLGTDTAGSGRVPAMLNNIVGLKPSLGLISTTGLVPACRTLDCISVFALTVDDAMIALRVMGTPDATDPYSRARPIAPM
SAMPDRPRLGVPRPDQLQFFGDQQSEQAYADALQRWTSLGAELIEIDVAPLYETARLLYDGPWVAERYLAIRELIDATPD
ALHPVTRQITLGGKAISAADTFAALYRLQALRKLAEPAFAAIDALVLPTAPTAYTVEQVLADPVTLNSRLGTYTNFVNLL
DLCGLAIPASIRADGIPFGVTLLAPGGRDAELASLGRMFHADTALPMGATGVALPPLAALDADAGTDHIQIAVVGAHLSG
MALNGELTSLDGRLLRATATAPDYKLYALNGTVPPKPGMLRVAAGAGAAIALEIWSLSPAAFGRFVAAIPQPLSIGTLKL
ADGALVKGFLVEPAALEVARDITHFGGWRAYMAELAKAG

Sequences:

>Translated_599_residues
MTETIADILAAHRAGTTTPAQTIARCYQRIRAHADPALFITLRDEADAVAEAVALAARDPSLPLYGVPVAVKDNIDVAGL
PTTAACPAFAYQPAQDSTAVAKLRAAGAIIIGKTNLDQFATGLVGVRSPYGIPRNAMRADLVPGGSSSGSAVAVGAGLVP
LSLGTDTAGSGRVPAMLNNIVGLKPSLGLISTTGLVPACRTLDCISVFALTVDDAMIALRVMGTPDATDPYSRARPIAPM
SAMPDRPRLGVPRPDQLQFFGDQQSEQAYADALQRWTSLGAELIEIDVAPLYETARLLYDGPWVAERYLAIRELIDATPD
ALHPVTRQITLGGKAISAADTFAALYRLQALRKLAEPAFAAIDALVLPTAPTAYTVEQVLADPVTLNSRLGTYTNFVNLL
DLCGLAIPASIRADGIPFGVTLLAPGGRDAELASLGRMFHADTALPMGATGVALPPLAALDADAGTDHIQIAVVGAHLSG
MALNGELTSLDGRLLRATATAPDYKLYALNGTVPPKPGMLRVAAGAGAAIALEIWSLSPAAFGRFVAAIPQPLSIGTLKL
ADGALVKGFLVEPAALEVARDITHFGGWRAYMAELAKAG
>Mature_598_residues
TETIADILAAHRAGTTTPAQTIARCYQRIRAHADPALFITLRDEADAVAEAVALAARDPSLPLYGVPVAVKDNIDVAGLP
TTAACPAFAYQPAQDSTAVAKLRAAGAIIIGKTNLDQFATGLVGVRSPYGIPRNAMRADLVPGGSSSGSAVAVGAGLVPL
SLGTDTAGSGRVPAMLNNIVGLKPSLGLISTTGLVPACRTLDCISVFALTVDDAMIALRVMGTPDATDPYSRARPIAPMS
AMPDRPRLGVPRPDQLQFFGDQQSEQAYADALQRWTSLGAELIEIDVAPLYETARLLYDGPWVAERYLAIRELIDATPDA
LHPVTRQITLGGKAISAADTFAALYRLQALRKLAEPAFAAIDALVLPTAPTAYTVEQVLADPVTLNSRLGTYTNFVNLLD
LCGLAIPASIRADGIPFGVTLLAPGGRDAELASLGRMFHADTALPMGATGVALPPLAALDADAGTDHIQIAVVGAHLSGM
ALNGELTSLDGRLLRATATAPDYKLYALNGTVPPKPGMLRVAAGAGAAIALEIWSLSPAAFGRFVAAIPQPLSIGTLKLA
DGALVKGFLVEPAALEVARDITHFGGWRAYMAELAKAG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=25.5905511811024, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI195972892, Length=192, Percent_Identity=30.7291666666667, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=27.9379157427938, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=169, Percent_Identity=35.5029585798817, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=265, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=306, Percent_Identity=27.1241830065359, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=173, Percent_Identity=30.635838150289, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=552, Percent_Identity=45.4710144927536, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323950, Length=248, Percent_Identity=30.241935483871, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648113, Length=252, Percent_Identity=34.9206349206349, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24652985, Length=419, Percent_Identity=24.3436754176611, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922090, Length=419, Percent_Identity=24.3436754176611, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652981, Length=419, Percent_Identity=24.3436754176611, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652983, Length=419, Percent_Identity=24.3436754176611, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 62133; Mature: 62002

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIADILAAHRAGTTTPAQTIARCYQRIRAHADPALFITLRDEADAVAEAVALAARDP
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCC
SLPLYGVPVAVKDNIDVAGLPTTAACPAFAYQPAQDSTAVAKLRAAGAIIIGKTNLDQFA
CCCEEECCEEEECCCCCCCCCCHHCCCCCEECCCCCCHHHHHHHHCCEEEEECCCHHHHH
TGLVGVRSPYGIPRNAMRADLVPGGSSSGSAVAVGAGLVPLSLGTDTAGSGRVPAMLNNI
HHHHCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHHHH
VGLKPSLGLISTTGLVPACRTLDCISVFALTVDDAMIALRVMGTPDATDPYSRARPIAPM
HCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHCCCCCCC
SAMPDRPRLGVPRPDQLQFFGDQQSEQAYADALQRWTSLGAELIEIDVAPLYETARLLYD
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCC
GPWVAERYLAIRELIDATPDALHPVTRQITLGGKAISAADTFAALYRLQALRKLAEPAFA
CCHHHHHHHHHHHHHHCCCHHHCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALVLPTAPTAYTVEQVLADPVTLNSRLGTYTNFVNLLDLCGLAIPASIRADGIPFGV
HHHHHEECCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
TLLAPGGRDAELASLGRMFHADTALPMGATGVALPPLAALDADAGTDHIQIAVVGAHLSG
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEECCCC
MALNGELTSLDGRLLRATATAPDYKLYALNGTVPPKPGMLRVAAGAGAAIALEIWSLSPA
EEECCEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCHH
AFGRFVAAIPQPLSIGTLKLADGALVKGFLVEPAALEVARDITHFGGWRAYMAELAKAG
HHHHHHHHCCCCCCCCEEEECCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TETIADILAAHRAGTTTPAQTIARCYQRIRAHADPALFITLRDEADAVAEAVALAARDP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCC
SLPLYGVPVAVKDNIDVAGLPTTAACPAFAYQPAQDSTAVAKLRAAGAIIIGKTNLDQFA
CCCEEECCEEEECCCCCCCCCCHHCCCCCEECCCCCCHHHHHHHHCCEEEEECCCHHHHH
TGLVGVRSPYGIPRNAMRADLVPGGSSSGSAVAVGAGLVPLSLGTDTAGSGRVPAMLNNI
HHHHCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHHHHH
VGLKPSLGLISTTGLVPACRTLDCISVFALTVDDAMIALRVMGTPDATDPYSRARPIAPM
HCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHCCCCCCC
SAMPDRPRLGVPRPDQLQFFGDQQSEQAYADALQRWTSLGAELIEIDVAPLYETARLLYD
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCC
GPWVAERYLAIRELIDATPDALHPVTRQITLGGKAISAADTFAALYRLQALRKLAEPAFA
CCHHHHHHHHHHHHHHCCCHHHCHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALVLPTAPTAYTVEQVLADPVTLNSRLGTYTNFVNLLDLCGLAIPASIRADGIPFGV
HHHHHEECCCCCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
TLLAPGGRDAELASLGRMFHADTALPMGATGVALPPLAALDADAGTDHIQIAVVGAHLSG
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEECCCC
MALNGELTSLDGRLLRATATAPDYKLYALNGTVPPKPGMLRVAAGAGAAIALEIWSLSPA
EEECCEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCCCEEEEEEECCCHH
AFGRFVAAIPQPLSIGTLKLADGALVKGFLVEPAALEVARDITHFGGWRAYMAELAKAG
HHHHHHHHCCCCCCCCEEEECCCCEEHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA