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The map label for this gene is bioH [H]

Identifier: 86751069

GI number: 86751069

Start: 4522734

End: 4523516

Strand: Direct

Name: bioH [H]

Synonym: RPB_3961

Alternate gene names: 86751069

Gene position: 4522734-4523516 (Clockwise)

Preceding gene: 86751068

Following gene: 86751076

Centisome position: 84.83

GC content: 67.69

Gene sequence:

>783_bases
ATGCAGATCGAGGTCAATGGGATCGACAGCTTCGTCGCCACCGGCGGCAAGCCGTTCGATGCGTCACTTCCCGCCGCCGT
GTTCATCCACGGCGCGGGGTTCGATCATTCGGCATGGGCGCTGCAGACGCGCTGGTTCGCGCATCACGGCTACGCGGTGT
TGGCGCCGGATCTGCCCGGCCACGGCCGTTCGGGCAGCGCGCCGCTGAAGACCATCGCCGAGATGGCCGACTGGATCGCA
GCGCTGCTCGATGCGGCCGGCGCGCAGCCGGCGAAGCTGATCGGCCATTCGATGGGCTCGCTGATTGCGCTGGAAACCGC
CGCGCGGCATCCGGCCAAGGTCGCGGCCTTGGCACTGATCGGCACCACAGCGACGATGACGGTCGGCCCCGATCTGTTGA
AGGCCGCGGAGGCGAATGATCCCGCCGCGTTCGCCATGATGACGATCTGGGGCCTCGGCCCCGACGCCGAGATCGGCGGC
AACCTCGCGCCCGGCCTGTGGATGCATGGCGGCTCGGTACGCGTCCTCGAGGCCAACAAGCCCGGCGTCATCTTCAACGA
TCTGTCGGCCTGTAACGACTACAAGGATGCGCTGGCGGCGGCGGCGAAGGTGACCGTGCCGACGACGCTCATCCTCGGCG
AACGTGACATGATGACGCCGACCAAGAACGGCAAGACGTTGGCGGCGGCGATCGCGGGCTCACGCACCATGATCCTGAAG
GGCGCCGGCCATACGATGATGGTGGAGCGCCCGGACGAGGTGCTGAAGGCGTTGCAGGGGTAG

Upstream 100 bases:

>100_bases
CGGTGAGGAACTGATCCGCCTCTCGGTCGGGATCGAAACCGCCGACGACATCATCGCTGATCTGGCGCAGGCGCTGCGCA
TTTCGCAGAAGGGCTGAGCC

Downstream 100 bases:

>100_bases
CTATCAACCTAGAGGCCGTCATTCCGGGGCGCTCGGGCGAGCGAGCGAACCCGGAATCCATAACCACCGATTTCGCGTTC
AAGCACGGCGTCGCAACGCC

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQIEVNGIDSFVATGGKPFDASLPAAVFIHGAGFDHSAWALQTRWFAHHGYAVLAPDLPGHGRSGSAPLKTIAEMADWIA
ALLDAAGAQPAKLIGHSMGSLIALETAARHPAKVAALALIGTTATMTVGPDLLKAAEANDPAAFAMMTIWGLGPDAEIGG
NLAPGLWMHGGSVRVLEANKPGVIFNDLSACNDYKDALAAAAKVTVPTTLILGERDMMTPTKNGKTLAAAIAGSRTMILK
GAGHTMMVERPDEVLKALQG

Sequences:

>Translated_260_residues
MQIEVNGIDSFVATGGKPFDASLPAAVFIHGAGFDHSAWALQTRWFAHHGYAVLAPDLPGHGRSGSAPLKTIAEMADWIA
ALLDAAGAQPAKLIGHSMGSLIALETAARHPAKVAALALIGTTATMTVGPDLLKAAEANDPAAFAMMTIWGLGPDAEIGG
NLAPGLWMHGGSVRVLEANKPGVIFNDLSACNDYKDALAAAAKVTVPTTLILGERDMMTPTKNGKTLAAAIAGSRTMILK
GAGHTMMVERPDEVLKALQG
>Mature_260_residues
MQIEVNGIDSFVATGGKPFDASLPAAVFIHGAGFDHSAWALQTRWFAHHGYAVLAPDLPGHGRSGSAPLKTIAEMADWIA
ALLDAAGAQPAKLIGHSMGSLIALETAARHPAKVAALALIGTTATMTVGPDLLKAAEANDPAAFAMMTIWGLGPDAEIGG
NLAPGLWMHGGSVRVLEANKPGVIFNDLSACNDYKDALAAAAKVTVPTTLILGERDMMTPTKNGKTLAAAIAGSRTMILK
GAGHTMMVERPDEVLKALQG

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 26804; Mature: 26804

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIEVNGIDSFVATGGKPFDASLPAAVFIHGAGFDHSAWALQTRWFAHHGYAVLAPDLPG
CEEEECCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHEEEEEEEEECCEEEEECCCCC
HGRSGSAPLKTIAEMADWIAALLDAAGAQPAKLIGHSMGSLIALETAARHPAKVAALALI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEHHHCCCCHHHEEEEEE
GTTATMTVGPDLLKAAEANDPAAFAMMTIWGLGPDAEIGGNLAPGLWMHGGSVRVLEANK
CCCEEEEECHHHHHHCCCCCCHHEEEEEEECCCCCCCCCCCCCCCEEEECCEEEEEECCC
PGVIFNDLSACNDYKDALAAAAKVTVPTTLILGERDMMTPTKNGKTLAAAIAGSRTMILK
CCEEEECHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEE
GAGHTMMVERPDEVLKALQG
CCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MQIEVNGIDSFVATGGKPFDASLPAAVFIHGAGFDHSAWALQTRWFAHHGYAVLAPDLPG
CEEEECCCHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHEEEEEEEEECCEEEEECCCCC
HGRSGSAPLKTIAEMADWIAALLDAAGAQPAKLIGHSMGSLIALETAARHPAKVAALALI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEHHHCCCCHHHEEEEEE
GTTATMTVGPDLLKAAEANDPAAFAMMTIWGLGPDAEIGGNLAPGLWMHGGSVRVLEANK
CCCEEEEECHHHHHHCCCCCCHHEEEEEEECCCCCCCCCCCCCCCEEEECCEEEEEECCC
PGVIFNDLSACNDYKDALAAAAKVTVPTTLILGERDMMTPTKNGKTLAAAIAGSRTMILK
CCEEEECHHHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEE
GAGHTMMVERPDEVLKALQG
CCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]