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The map label for this gene is mdeA [H]

Identifier: 86751068

GI number: 86751068

Start: 4521429

End: 4522730

Strand: Direct

Name: mdeA [H]

Synonym: RPB_3960

Alternate gene names: 86751068

Gene position: 4521429-4522730 (Clockwise)

Preceding gene: 86751067

Following gene: 86751069

Centisome position: 84.8

GC content: 65.21

Gene sequence:

>1302_bases
ATGCTCATGGCCGCTCCAAAACCGCCCGGATTCGAGACACTTAGCCTGCACGCCGGGCAACACCCCGACCCGCTGACCGG
CTCGCGCGCGGTGCCGATCTATCAGACCACGTCCTACGTCTTCCAGGACACCGACCACGCCGCCGCTTTGTTCAACATGG
AGCGGCCCGGGCATCTCTACACGCGCATCTCCAACCCGACCACCGCGGTGCTGGAAGAGCGTATCGCCGCGCTCGAAAAC
GGCGTCGGCGCGGTCGCCACCGCGAGCGGCATGGCGGCGCTGCATCTGGCGATCGCGACGCTGCTCAACGCCGGCGATCA
CATCGTCGCGTCGTCGTCGCTGTACGGCGGCACGATCAACCTTCTCGCGCACACGCTGCCGCGCTTCGGCATCACCACGA
CTTTTGTGAAGCCGCGCGACCACGACGGCCTCGCGGCCGCCATCAAGCCGAATACGCGGCTGATGATCGGTGAGACCATC
GGCAATCCCGGCCTCGAAGTGCTCGACATTCCGAAGGTCGCGGCGATTGCGCATGAGGCTAAGATCCCCTTGCTGATCGA
CAACACCTTTGCGACGCCCTATCTGAGCCAGCCGATCGCGCTCGGCGCCGATATCGTGATGCATTCGGCGACCAAATGGC
TCGGCGGCCACGGCATCGCGATCGGCGGCGTGTTGGTCGACGGCGGCCGTTTCGACTGGCGCGGCTCGGGCAAATTCCCG
ACGCTGACCGAACCCTATGCCGGCTATCACGACATCGTCTTCGACGAACAATTCGGGCCGCCGGCCTTTATCATTCGCGC
GCGAATGGAAGGGCTGCGCGACTTTGGAGCTTGTCTGTCGCCGACCAATGCGTTCCAGCTGCTGCAGGGCGTGGAAACGC
TGTCGGTGCGGATGGATCGGCACGTCGCCAACACCGCAGCGGTGCTCGACTTTCTCGGCGGCAACAAGGCGGTCGAGTGG
GTGCTGCATCCGACGCTGGAAAGCCATCCGGACTACGCACTCGCGAAAGAGCTGCTGCCGAAGGGCGCCGGCTCGATCGT
CTCGTTCGGCATCAAGGGCGGCCGCGCCGCCGGGCGCAAATTCATCGAAGCGCTCAAGCTGACCAGCCACCTCGCCAATG
TCGGCGACGCCAAGACGCTGGTGATCCACCCGGCCTCGACGACGCATCAGCAGATGAGCGCGGAGCAGTTGACGGCCGCC
GGGATCGGTGAGGAACTGATCCGCCTCTCGGTCGGGATCGAAACCGCCGACGACATCATCGCTGATCTGGCGCAGGCGCT
GCGCATTTCGCAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CGCGATCGTCTACGCCTTCGCCTTGTGGGCGGTCGGCCTCGCGGATTCCGCTGCCTGAACTTCGCCACCAGTTTGCCGCA
ACCCTCCTTCAGCCGCAGAG

Downstream 100 bases:

>100_bases
GCCATGCAGATCGAGGTCAATGGGATCGACAGCTTCGTCGCCACCGGCGGCAAGCCGTTCGATGCGTCACTTCCCGCCGC
CGTGTTCATCCACGGCGCGG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLMAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTTAVLEERIAALEN
GVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVKPRDHDGLAAAIKPNTRLMIGETI
GNPGLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSQPIALGADIVMHSATKWLGGHGIAIGGVLVDGGRFDWRGSGKFP
TLTEPYAGYHDIVFDEQFGPPAFIIRARMEGLRDFGACLSPTNAFQLLQGVETLSVRMDRHVANTAAVLDFLGGNKAVEW
VLHPTLESHPDYALAKELLPKGAGSIVSFGIKGGRAAGRKFIEALKLTSHLANVGDAKTLVIHPASTTHQQMSAEQLTAA
GIGEELIRLSVGIETADDIIADLAQALRISQKG

Sequences:

>Translated_433_residues
MLMAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTTAVLEERIAALEN
GVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVKPRDHDGLAAAIKPNTRLMIGETI
GNPGLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSQPIALGADIVMHSATKWLGGHGIAIGGVLVDGGRFDWRGSGKFP
TLTEPYAGYHDIVFDEQFGPPAFIIRARMEGLRDFGACLSPTNAFQLLQGVETLSVRMDRHVANTAAVLDFLGGNKAVEW
VLHPTLESHPDYALAKELLPKGAGSIVSFGIKGGRAAGRKFIEALKLTSHLANVGDAKTLVIHPASTTHQQMSAEQLTAA
GIGEELIRLSVGIETADDIIADLAQALRISQKG
>Mature_433_residues
MLMAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLYTRISNPTTAVLEERIAALEN
GVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTINLLAHTLPRFGITTTFVKPRDHDGLAAAIKPNTRLMIGETI
GNPGLEVLDIPKVAAIAHEAKIPLLIDNTFATPYLSQPIALGADIVMHSATKWLGGHGIAIGGVLVDGGRFDWRGSGKFP
TLTEPYAGYHDIVFDEQFGPPAFIIRARMEGLRDFGACLSPTNAFQLLQGVETLSVRMDRHVANTAAVLDFLGGNKAVEW
VLHPTLESHPDYALAKELLPKGAGSIVSFGIKGGRAAGRKFIEALKLTSHLANVGDAKTLVIHPASTTHQQMSAEQLTAA
GIGEELIRLSVGIETADDIIADLAQALRISQKG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=35.3629976580796, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=32.3185011709602, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=30.9859154929577, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=32.3040380047506, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=25, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=35.9154929577465, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=429, Percent_Identity=33.1002331002331, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17560056, Length=426, Percent_Identity=34.7417840375587, Blast_Score=208, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=47.5862068965517, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=33.0275229357798, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=355, Percent_Identity=29.5774647887324, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=26.3982102908277, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=425, Percent_Identity=26.3529411764706, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=34.4186046511628, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45846; Mature: 45846

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLY
CCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCEE
TRISNPTTAVLEERIAALENGVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTIN
EEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
LLAHTLPRFGITTTFVKPRDHDGLAAAIKPNTRLMIGETIGNPGLEVLDIPKVAAIAHEA
HHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHHC
KIPLLIDNTFATPYLSQPIALGADIVMHSATKWLGGHGIAIGGVLVDGGRFDWRGSGKFP
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEECCCCCCCC
TLTEPYAGYHDIVFDEQFGPPAFIIRARMEGLRDFGACLSPTNAFQLLQGVETLSVRMDR
CCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HVANTAAVLDFLGGNKAVEWVLHPTLESHPDYALAKELLPKGAGSIVSFGIKGGRAAGRK
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHH
FIEALKLTSHLANVGDAKTLVIHPASTTHQQMSAEQLTAAGIGEELIRLSVGIETADDII
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHH
ADLAQALRISQKG
HHHHHHHHHCCCC
>Mature Secondary Structure
MLMAAPKPPGFETLSLHAGQHPDPLTGSRAVPIYQTTSYVFQDTDHAAALFNMERPGHLY
CCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCHHEEECCCCCEEEEECCCCCCEE
TRISNPTTAVLEERIAALENGVGAVATASGMAALHLAIATLLNAGDHIVASSSLYGGTIN
EEECCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHH
LLAHTLPRFGITTTFVKPRDHDGLAAAIKPNTRLMIGETIGNPGLEVLDIPKVAAIAHEA
HHHHHHCCCCCEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCEEEECCHHHHHHHHC
KIPLLIDNTFATPYLSQPIALGADIVMHSATKWLGGHGIAIGGVLVDGGRFDWRGSGKFP
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEECCCCCCCC
TLTEPYAGYHDIVFDEQFGPPAFIIRARMEGLRDFGACLSPTNAFQLLQGVETLSVRMDR
CCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HVANTAAVLDFLGGNKAVEWVLHPTLESHPDYALAKELLPKGAGSIVSFGIKGGRAAGRK
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHH
FIEALKLTSHLANVGDAKTLVIHPASTTHQQMSAEQLTAAGIGEELIRLSVGIETADDII
HHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHH
ADLAQALRISQKG
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]