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The map label for this gene is rutF [H]

Identifier: 86751006

GI number: 86751006

Start: 4453463

End: 4453993

Strand: Direct

Name: rutF [H]

Synonym: RPB_3898

Alternate gene names: 86751006

Gene position: 4453463-4453993 (Clockwise)

Preceding gene: 86751005

Following gene: 86751007

Centisome position: 83.53

GC content: 68.93

Gene sequence:

>531_bases
ATGAAGCAGGCCATGACGCCCACGGTCGAGCGCGACGAATTTCGCGAGGCGATGAGCCGGCTCGGCGCCGCGGTCAACAT
CATCACCACCGACGGCGCGGCCGGGCTGCACGGCTTGACCGCCTCGGCGGTGTGCAGTGTCACCGACGATCCGCCGACGT
TGCTGGTCTGCGTCAACCGCGCCTCCAACGTCCATGCGGCGCTGTCGCAGAACGGCGTGCTGTGCGTCAACGTGCTGGCG
AGCCGGCATCAGGAATTGTCGACTCTGTTCGGTCGCGGCGGCATCCCGGTGGAGCAGCGCTTCGCCGGAGCCGAATGGCG
GGTGCTGACGACCGGCGCGCCATCGCTCGCCGACGCCGTGGTCAGCCTCGATTGCGCGATCGCGCAGACCACCGAGATCG
GCACCCACAGCGTGTTCTTCTGCCGCGTCCGGGCGATCGCGCTCGGCGCGCAGCCGGAAGGGCTGATCTACTTCAACCGC
TCCTATCACGACCTCGCGCCGGCGCAGCGCGACCTGTGCCACCAGGACTGA

Upstream 100 bases:

>100_bases
TCGCGCAGCTGGTGCTGCAACTGGCGTCGCCCGAATCCGGCTTCGTCTCCGGCGCGACCTGGGACGTCAATGGCGGGCTG
TTCATGCGATGAGGCCGACG

Downstream 100 bases:

>100_bases
TCCCGCTTCGACCAGAGAAAGCCGCATCCCGATGAACATCGACCAACTCGCATCGACCGTGAAGGCCCTGCAGGACGAGA
TCGCCACGCTGCGCGCGGAG

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MKQAMTPTVERDEFREAMSRLGAAVNIITTDGAAGLHGLTASAVCSVTDDPPTLLVCVNRASNVHAALSQNGVLCVNVLA
SRHQELSTLFGRGGIPVEQRFAGAEWRVLTTGAPSLADAVVSLDCAIAQTTEIGTHSVFFCRVRAIALGAQPEGLIYFNR
SYHDLAPAQRDLCHQD

Sequences:

>Translated_176_residues
MKQAMTPTVERDEFREAMSRLGAAVNIITTDGAAGLHGLTASAVCSVTDDPPTLLVCVNRASNVHAALSQNGVLCVNVLA
SRHQELSTLFGRGGIPVEQRFAGAEWRVLTTGAPSLADAVVSLDCAIAQTTEIGTHSVFFCRVRAIALGAQPEGLIYFNR
SYHDLAPAQRDLCHQD
>Mature_176_residues
MKQAMTPTVERDEFREAMSRLGAAVNIITTDGAAGLHGLTASAVCSVTDDPPTLLVCVNRASNVHAALSQNGVLCVNVLA
SRHQELSTLFGRGGIPVEQRFAGAEWRVLTTGAPSLADAVVSLDCAIAQTTEIGTHSVFFCRVRAIALGAQPEGLIYFNR
SYHDLAPAQRDLCHQD

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=55.4140127388535, Blast_Score=179, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18731; Mature: 18731

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAMTPTVERDEFREAMSRLGAAVNIITTDGAAGLHGLTASAVCSVTDDPPTLLVCVNR
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEECC
ASNVHAALSQNGVLCVNVLASRHQELSTLFGRGGIPVEQRFAGAEWRVLTTGAPSLADAV
CCCEEEHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCHHHHHH
VSLDCAIAQTTEIGTHSVFFCRVRAIALGAQPEGLIYFNRSYHDLAPAQRDLCHQD
HHHHHHHHHCCCCCCCHHHHEEEEHHEECCCCCCEEEECCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKQAMTPTVERDEFREAMSRLGAAVNIITTDGAAGLHGLTASAVCSVTDDPPTLLVCVNR
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEECC
ASNVHAALSQNGVLCVNVLASRHQELSTLFGRGGIPVEQRFAGAEWRVLTTGAPSLADAV
CCCEEEHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCHHHHHH
VSLDCAIAQTTEIGTHSVFFCRVRAIALGAQPEGLIYFNRSYHDLAPAQRDLCHQD
HHHHHHHHHCCCCCCCHHHHEEEEHHEECCCCCCEEEECCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA