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The map label for this gene is yoaE [H]

Identifier: 86750527

GI number: 86750527

Start: 3903636

End: 3905762

Strand: Reverse

Name: yoaE [H]

Synonym: RPB_3416

Alternate gene names: 86750527

Gene position: 3905762-3903636 (Counterclockwise)

Preceding gene: 86750530

Following gene: 86750526

Centisome position: 73.26

GC content: 64.69

Gene sequence:

>2127_bases
ATGGCCCGGACCACAGTTGTAGGCGCGTGTCCACACGACTGCCCTGATACTTGCTCCATCATCACGACCGTCGAGGACGG
CAAGGCGATCGCCGTCCGCGGCAATCCGGATCACCCGTTCACGCGCGGACGGTTGTGCGTCAAGGTCAACAACTACGAGG
AGCGCGTCTACAGCGACAAGCGCGTCCTCTACCCGATGAAACGCGTCGGCCCCAAGGGCACCAAGCAGTTTCAGCGAATT
TCCTGGGACGAGGCGATCGCGACGATCGCGACCCGCTGGAAAGCGATCATCGCCGAGCACGGTGCGCAGGCGATCCTGCC
CTACAGCTATCTCGGCACCCAGGGCGTCATCAACGGGCTCAATGTCGGCGATCCGCTGTTCAACAAGCTCGGCGCCACTG
TGCTCGAACGCACCTTCTGCGACTCTGGTTCCTGTACGGCCTATCTGCTGACGATCGGGCACACGCCGGGTGTCGATCCC
GAGAGCTTCGTGCACTCGAAGTACATCATTCTATGGGCCTGCAATACGCTCAGCACCAATTCGCATCACTGGCCGTTCAT
CGAGCAGGCCAAGAAGAACGGCGCCAAGCTGGTGGTGATTGATCCGGTCAAGACACGGACCGCGCGGCTGGCGGACTGGC
ACATCCAGATCCGGCCTGGCACCGATGCGGCGCTGGCGCTGGCGATGATGCACGTGATCATCAAGGAGAACCTGGTCGAT
CGCGATTACATCGACAAGCACACCGTCGGGTTCGACGAACTGGCGGAGCGCGCCGCCACCTATACGCCGGAATTCGCCTC
GCAGGAGACCGGCATTCCGGTCGAGGATATCGTCAAGCTGGCGCGCGACTACGCCACGACGCCGCCCGCGGTGGTGCGGA
TCGGCGTCGCCGTGGAGCGCCATGCCGGCGGCGGCCAGACCGTGCGCGCGATCGCCTGCCTTCCCGGACTGATCGGTGCT
TGGAAGCACGTCGGTGGCGGCCTGCTGCAGCTGCCGATCTGGGCTTTCCCGGTCAAATGGGACGTGCTGATGCGCCCCGA
TCTTCAGCCGGAGAACATGCGCGTGCTGAATTCCTGGCGGCTCGGCGCGGCGCTGACCGGCGAACTCGGCTTCGATCCGC
CGATCAAGGCGCTGTTCGTCTACAACGCCAACCCGATGGCGATGGTGACCGAGCAGGACAAGATCGAGAAGGGGCTCGAA
CGCGAAGATTTATTCACGGTCGTCAGTGAGCATTTCATCACCGACACGGCGAAGTACGCCGACATCGTGCTGCCCGCGAC
CACGCAGCTCGAGCAGAAGGACATCATGTTCTCCTGGGGGCATCTGTATCTGTCCTACAACAACCAGGCGATCGAGCCGC
TCGGCGAGGCTGTGCCGAACACCGAACTGTTCCGGCGCCTCGCCAAGGCGATGGGGATCACCGATCCGGCGTTCTTCCGC
ACCGACGACGAGATGATCGAGGCGGCGCTGGACTGGAGCAGCCCGGTGCTGCAGGGGATCACGCTCGACGAGGTCAAGGC
CAAGGGCTATTCGCGGCTCGCCATGCCGGCGTCCGACGACTGGGCGCCGCACCGCGACGGCAATTTCCCGACTGCTACCG
GCAAATGCGAGTTCAAATCGACGATCGCCGCGGGCGGCAATTTCGTCGCGCCGCTGTTCCGGCAGGGCTACAACGGCGAC
CAGGACGGCACGCCGATCGATCCGTTGCCGCACTACATCGCGCCGAACGAGAGTGTCCGGACGGCGCCGGCGCTGGCGGC
GCGCTATCCGCTCAGCCTGATCTCGCCGAAAAGCCACGCTTTCCTCAATTCGAACTACGGCAACCTGCCGGCGCAGACCG
CGCAGGCGGGCGAAGAGCAGGCGGTGATCCTGAATCCGGACGACGCCGCGTTCCGCGGCATCGAAGCCGGCGCGCCGATC
CGCGTGTTCAACGATCGCGGCTCGTTCGAAGCCTATGCGACGGTGTCGTCCGATATCATGGCGGGGGTGGTGGTTGCGCC
GTCCGGCTACTGGCAGCGCTCCAATCGCAAAGGCGCGACCGTTCACGCGCTGACGCCGCCGGCCTTCGCCGATCTCGGCC
GTGCGCCGACGTTCTCGGACATTCTCGTCCAGGTCGGCCGACTGTAG

Upstream 100 bases:

>100_bases
AGCAGCCCAGGACAACAACGTAGCACGTTTTGAGAAGCCGAGGCCGCCTCCGTCGCCGCATCTTTAGGCATGTCACCGTT
TGGTGCAAGCGGAGGATCCC

Downstream 100 bases:

>100_bases
CCGGCGAGCGAACGACCGATGCTCCGGCGTCGGTCGATGTTGATCCTCATCGATACACGGGAAGTCGAACGATGACGTAT
GTGGTTACGGACAATTGCAA

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MARTTVVGACPHDCPDTCSIITTVEDGKAIAVRGNPDHPFTRGRLCVKVNNYEERVYSDKRVLYPMKRVGPKGTKQFQRI
SWDEAIATIATRWKAIIAEHGAQAILPYSYLGTQGVINGLNVGDPLFNKLGATVLERTFCDSGSCTAYLLTIGHTPGVDP
ESFVHSKYIILWACNTLSTNSHHWPFIEQAKKNGAKLVVIDPVKTRTARLADWHIQIRPGTDAALALAMMHVIIKENLVD
RDYIDKHTVGFDELAERAATYTPEFASQETGIPVEDIVKLARDYATTPPAVVRIGVAVERHAGGGQTVRAIACLPGLIGA
WKHVGGGLLQLPIWAFPVKWDVLMRPDLQPENMRVLNSWRLGAALTGELGFDPPIKALFVYNANPMAMVTEQDKIEKGLE
REDLFTVVSEHFITDTAKYADIVLPATTQLEQKDIMFSWGHLYLSYNNQAIEPLGEAVPNTELFRRLAKAMGITDPAFFR
TDDEMIEAALDWSSPVLQGITLDEVKAKGYSRLAMPASDDWAPHRDGNFPTATGKCEFKSTIAAGGNFVAPLFRQGYNGD
QDGTPIDPLPHYIAPNESVRTAPALAARYPLSLISPKSHAFLNSNYGNLPAQTAQAGEEQAVILNPDDAAFRGIEAGAPI
RVFNDRGSFEAYATVSSDIMAGVVVAPSGYWQRSNRKGATVHALTPPAFADLGRAPTFSDILVQVGRL

Sequences:

>Translated_708_residues
MARTTVVGACPHDCPDTCSIITTVEDGKAIAVRGNPDHPFTRGRLCVKVNNYEERVYSDKRVLYPMKRVGPKGTKQFQRI
SWDEAIATIATRWKAIIAEHGAQAILPYSYLGTQGVINGLNVGDPLFNKLGATVLERTFCDSGSCTAYLLTIGHTPGVDP
ESFVHSKYIILWACNTLSTNSHHWPFIEQAKKNGAKLVVIDPVKTRTARLADWHIQIRPGTDAALALAMMHVIIKENLVD
RDYIDKHTVGFDELAERAATYTPEFASQETGIPVEDIVKLARDYATTPPAVVRIGVAVERHAGGGQTVRAIACLPGLIGA
WKHVGGGLLQLPIWAFPVKWDVLMRPDLQPENMRVLNSWRLGAALTGELGFDPPIKALFVYNANPMAMVTEQDKIEKGLE
REDLFTVVSEHFITDTAKYADIVLPATTQLEQKDIMFSWGHLYLSYNNQAIEPLGEAVPNTELFRRLAKAMGITDPAFFR
TDDEMIEAALDWSSPVLQGITLDEVKAKGYSRLAMPASDDWAPHRDGNFPTATGKCEFKSTIAAGGNFVAPLFRQGYNGD
QDGTPIDPLPHYIAPNESVRTAPALAARYPLSLISPKSHAFLNSNYGNLPAQTAQAGEEQAVILNPDDAAFRGIEAGAPI
RVFNDRGSFEAYATVSSDIMAGVVVAPSGYWQRSNRKGATVHALTPPAFADLGRAPTFSDILVQVGRL
>Mature_707_residues
ARTTVVGACPHDCPDTCSIITTVEDGKAIAVRGNPDHPFTRGRLCVKVNNYEERVYSDKRVLYPMKRVGPKGTKQFQRIS
WDEAIATIATRWKAIIAEHGAQAILPYSYLGTQGVINGLNVGDPLFNKLGATVLERTFCDSGSCTAYLLTIGHTPGVDPE
SFVHSKYIILWACNTLSTNSHHWPFIEQAKKNGAKLVVIDPVKTRTARLADWHIQIRPGTDAALALAMMHVIIKENLVDR
DYIDKHTVGFDELAERAATYTPEFASQETGIPVEDIVKLARDYATTPPAVVRIGVAVERHAGGGQTVRAIACLPGLIGAW
KHVGGGLLQLPIWAFPVKWDVLMRPDLQPENMRVLNSWRLGAALTGELGFDPPIKALFVYNANPMAMVTEQDKIEKGLER
EDLFTVVSEHFITDTAKYADIVLPATTQLEQKDIMFSWGHLYLSYNNQAIEPLGEAVPNTELFRRLAKAMGITDPAFFRT
DDEMIEAALDWSSPVLQGITLDEVKAKGYSRLAMPASDDWAPHRDGNFPTATGKCEFKSTIAAGGNFVAPLFRQGYNGDQ
DGTPIDPLPHYIAPNESVRTAPALAARYPLSLISPKSHAFLNSNYGNLPAQTAQAGEEQAVILNPDDAAFRGIEAGAPIR
VFNDRGSFEAYATVSSDIMAGVVVAPSGYWQRSNRKGATVHALTPPAFADLGRAPTFSDILVQVGRL

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=784, Percent_Identity=29.719387755102, Blast_Score=245, Evalue=8e-66,
Organism=Escherichia coli, GI1787870, Length=756, Percent_Identity=30.1587301587302, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI171474008, Length=776, Percent_Identity=28.7371134020619, Blast_Score=215, Evalue=1e-56,
Organism=Escherichia coli, GI3868721, Length=714, Percent_Identity=25.0700280112045, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1787231, Length=782, Percent_Identity=24.9360613810742, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI145693196, Length=728, Percent_Identity=23.7637362637363, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI87081994, Length=713, Percent_Identity=23.8429172510519, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787477, Length=287, Percent_Identity=25.4355400696864, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787741, Length=289, Percent_Identity=24.9134948096886, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 77415; Mature: 77284

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTTVVGACPHDCPDTCSIITTVEDGKAIAVRGNPDHPFTRGRLCVKVNNYEERVYSDK
CCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHCCC
RVLYPMKRVGPKGTKQFQRISWDEAIATIATRWKAIIAEHGAQAILPYSYLGTQGVINGL
CEECCHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCHHCCCCC
NVGDPLFNKLGATVLERTFCDSGSCTAYLLTIGHTPGVDPESFVHSKYIILWACNTLSTN
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCC
SHHWPFIEQAKKNGAKLVVIDPVKTRTARLADWHIQIRPGTDAALALAMMHVIIKENLVD
CCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
RDYIDKHTVGFDELAERAATYTPEFASQETGIPVEDIVKLARDYATTPPAVVRIGVAVER
CHHCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEEE
HAGGGQTVRAIACLPGLIGAWKHVGGGLLQLPIWAFPVKWDVLMRPDLQPENMRVLNSWR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEECEEEEEEECCCCCCCCCEEEEEEC
LGAALTGELGFDPPIKALFVYNANPMAMVTEQDKIEKGLEREDLFTVVSEHFITDTAKYA
CCEEEECCCCCCCCCEEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCHHHC
DIVLPATTQLEQKDIMFSWGHLYLSYNNQAIEPLGEAVPNTELFRRLAKAMGITDPAFFR
EEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHCC
TDDEMIEAALDWSSPVLQGITLDEVKAKGYSRLAMPASDDWAPHRDGNFPTATGKCEFKS
CCHHHHHHHHCCCCHHHCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHH
TIAAGGNFVAPLFRQGYNGDQDGTPIDPLPHYIAPNESVRTAPALAARYPLSLISPKSHA
HHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCE
FLNSNYGNLPAQTAQAGEEQAVILNPDDAAFRGIEAGAPIRVFNDRGSFEAYATVSSDIM
EECCCCCCCCCHHHCCCCCCEEEECCCHHHHCCCCCCCCEEEECCCCCEEEEEEECCCHH
AGVVVAPSGYWQRSNRKGATVHALTPPAFADLGRAPTFSDILVQVGRL
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
ARTTVVGACPHDCPDTCSIITTVEDGKAIAVRGNPDHPFTRGRLCVKVNNYEERVYSDK
CCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCEEEEEECCHHHHHHCCC
RVLYPMKRVGPKGTKQFQRISWDEAIATIATRWKAIIAEHGAQAILPYSYLGTQGVINGL
CEECCHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCHHCCCCC
NVGDPLFNKLGATVLERTFCDSGSCTAYLLTIGHTPGVDPESFVHSKYIILWACNTLSTN
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHCCCEEEEEEECCCCCC
SHHWPFIEQAKKNGAKLVVIDPVKTRTARLADWHIQIRPGTDAALALAMMHVIIKENLVD
CCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
RDYIDKHTVGFDELAERAATYTPEFASQETGIPVEDIVKLARDYATTPPAVVRIGVAVER
CHHCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEEE
HAGGGQTVRAIACLPGLIGAWKHVGGGLLQLPIWAFPVKWDVLMRPDLQPENMRVLNSWR
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCEEEECEEEEEEECCCCCCCCCEEEEEEC
LGAALTGELGFDPPIKALFVYNANPMAMVTEQDKIEKGLEREDLFTVVSEHFITDTAKYA
CCEEEECCCCCCCCCEEEEEECCCCEEEEECHHHHHHCCCHHHHHHHHHHHHHHCCHHHC
DIVLPATTQLEQKDIMFSWGHLYLSYNNQAIEPLGEAVPNTELFRRLAKAMGITDPAFFR
EEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCHHCC
TDDEMIEAALDWSSPVLQGITLDEVKAKGYSRLAMPASDDWAPHRDGNFPTATGKCEFKS
CCHHHHHHHHCCCCHHHCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHH
TIAAGGNFVAPLFRQGYNGDQDGTPIDPLPHYIAPNESVRTAPALAARYPLSLISPKSHA
HHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCE
FLNSNYGNLPAQTAQAGEEQAVILNPDDAAFRGIEAGAPIRVFNDRGSFEAYATVSSDIM
EECCCCCCCCCHHHCCCCCCEEEECCCHHHHCCCCCCCCEEEECCCCCEEEEEEECCCHH
AGVVVAPSGYWQRSNRKGATVHALTPPAFADLGRAPTFSDILVQVGRL
EEEEECCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]