Click here to switch to the map view.

The map label for this gene is appA [H]

Identifier: 86750244

GI number: 86750244

Start: 3579560

End: 3581149

Strand: Reverse

Name: appA [H]

Synonym: RPB_3133

Alternate gene names: 86750244

Gene position: 3581149-3579560 (Counterclockwise)

Preceding gene: 86750245

Following gene: 86750243

Centisome position: 67.17

GC content: 62.7

Gene sequence:

>1590_bases
ATGAACAGACGCGAATTCACCAAGCTCGGCCTGCTGGCCGGCGCGGCCGGCATCGGCGGCATTCCGCTCGGCATCACCCG
GGCGGTGGGGCAAACCCGCGGCGGCACGCTCAACACCATCATTCAGCCGGAGCCGCCGATCCTGGTCACCGCGCTCAACC
AGCAGCAGCCGACGCTGACGCTCGGCGGCAAGATCTACGAAAGCCTGCTGCGCTACGATTTCGATCTCAAGCCGCTGCCC
GGCCTGGCGCAGTCCTGGGAAGTGTCGCCCGACAAGCTGACCTATACATTCAAGCTGCATCCCAACATCACCTTCCACGA
CGGCGCGCCGCTGACGTCCGAAGACGTGGTGTTCTCGATCATGAAGGTGCTGATCGAGAACCACGCCCGCGCGCGCAACA
CGTTCTCGCGCGTCGAGAAGGCCGAGGCGCCGGATCCGCTGACCGTGGTGTTCAAACTGAAGGCGCCGTTCGCGCCGTTC
CTCACCGCGTTCGACTGCACCACGGCCCCGATCGTGCCGAAGCATATTTACGAAGGCACGGACTATCGCAAGAACCCGGC
CAATGCGAAGGCGATCGGCTCGGGTCCGTTCAAGCTGAAGGAATGGGTGCGCGGTTCGCACGTCCATCTGGTCAAGCACG
AAGGCTATTATCGCCCGGGCGAGCCGGTTCTCGACGAGATTATCTATCGGGTCATCCCGGACTCGGCGTCGCGCTCGGTG
GCGCTGGAGCAGGGGACCGTGCAGCTCACGCAATGGACCGACGTCGAACTGTTCGAGGTGCCGCGGCTGTCCAAGCTGCC
GCATCTGACGATGACCACCAAGGGCTACGAATTCTTCGCCCCCCATACGTGGCTCGAGTTCAACACCCGGATCGCGCCGA
TGAACGACAAGCGGTTCCGGCAGGCGGTGATGTATGCGATCGACCGCAAGGCGTTGCTGGCCCGCATCTATTTCGGTCTC
GGCAAGGTCGCGACCGGCCCGGTGTCGTCGAAGACCAAATTCTACGAGAAGAACGTCAAGGCCTACGACTTCTCGCCCGA
GAAGGCGAAGGCTCTGCTCGACGAGATGGGGCTGAAGCCGGGTGCCGACGGCAAGCGCGTGACGATTCCCTATCTCGTGC
CGCCCTACGGTGAAACGCATCAACGGACCTCCGAATTCATTCGCCAGTCGCTCGCCCGCGTCGGCATCGACCTGCAACTC
CAGGGGATCGATGTCGCCGGATGGGCCGAGAAGTTCAGCAACTGGGACTTCTCGATGACGGCGACCACGGTCTATCAGTT
CGGCGATCCGGCGCTCGGCGTGTCACGGAGTTATGTCTCCTCCAACATCCGCAAGGGCATTCTGTTCTCCAACACCTGCG
GCTACTCCAATCCGGAGGTCGACCGGCTGTTCGAGGAGGCCGCGGTCGCGACCTCGGACGACAAGCGCCAGGAGTTCTAC
AGCGAGGTTCAGAAGATCATGGTCGAGGACGTGCCGGTCGCCTGGCTGCTCGAGATCGACTATCCGAACTTCATGGACAA
ACGTCTGAAGAACGTGGTGACGACGGCGATCGGCGTGCACGACACGTTCGGGACGGTTTCGTTCGGATGA

Upstream 100 bases:

>100_bases
GTGATCGAGGCAATGGTGTGATGAGGAGGTCGGTGTCGATGCCATCTTCGACGCCGCCGGCGAACGATCTGGTTCTATTC
AACTCAATGCGGGGGATCTC

Downstream 100 bases:

>100_bases
GAGCGCTGGCGCTGTCCATCACCGGCAACCGAGGCGTGGCGATCGCGATCGCGATCGTCCAGTGGGTGGCGCGGTTCGTC
GCGGTGGTGCTGGTCATCGC

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNRREFTKLGLLAGAAGIGGIPLGITRAVGQTRGGTLNTIIQPEPPILVTALNQQQPTLTLGGKIYESLLRYDFDLKPLP
GLAQSWEVSPDKLTYTFKLHPNITFHDGAPLTSEDVVFSIMKVLIENHARARNTFSRVEKAEAPDPLTVVFKLKAPFAPF
LTAFDCTTAPIVPKHIYEGTDYRKNPANAKAIGSGPFKLKEWVRGSHVHLVKHEGYYRPGEPVLDEIIYRVIPDSASRSV
ALEQGTVQLTQWTDVELFEVPRLSKLPHLTMTTKGYEFFAPHTWLEFNTRIAPMNDKRFRQAVMYAIDRKALLARIYFGL
GKVATGPVSSKTKFYEKNVKAYDFSPEKAKALLDEMGLKPGADGKRVTIPYLVPPYGETHQRTSEFIRQSLARVGIDLQL
QGIDVAGWAEKFSNWDFSMTATTVYQFGDPALGVSRSYVSSNIRKGILFSNTCGYSNPEVDRLFEEAAVATSDDKRQEFY
SEVQKIMVEDVPVAWLLEIDYPNFMDKRLKNVVTTAIGVHDTFGTVSFG

Sequences:

>Translated_529_residues
MNRREFTKLGLLAGAAGIGGIPLGITRAVGQTRGGTLNTIIQPEPPILVTALNQQQPTLTLGGKIYESLLRYDFDLKPLP
GLAQSWEVSPDKLTYTFKLHPNITFHDGAPLTSEDVVFSIMKVLIENHARARNTFSRVEKAEAPDPLTVVFKLKAPFAPF
LTAFDCTTAPIVPKHIYEGTDYRKNPANAKAIGSGPFKLKEWVRGSHVHLVKHEGYYRPGEPVLDEIIYRVIPDSASRSV
ALEQGTVQLTQWTDVELFEVPRLSKLPHLTMTTKGYEFFAPHTWLEFNTRIAPMNDKRFRQAVMYAIDRKALLARIYFGL
GKVATGPVSSKTKFYEKNVKAYDFSPEKAKALLDEMGLKPGADGKRVTIPYLVPPYGETHQRTSEFIRQSLARVGIDLQL
QGIDVAGWAEKFSNWDFSMTATTVYQFGDPALGVSRSYVSSNIRKGILFSNTCGYSNPEVDRLFEEAAVATSDDKRQEFY
SEVQKIMVEDVPVAWLLEIDYPNFMDKRLKNVVTTAIGVHDTFGTVSFG
>Mature_529_residues
MNRREFTKLGLLAGAAGIGGIPLGITRAVGQTRGGTLNTIIQPEPPILVTALNQQQPTLTLGGKIYESLLRYDFDLKPLP
GLAQSWEVSPDKLTYTFKLHPNITFHDGAPLTSEDVVFSIMKVLIENHARARNTFSRVEKAEAPDPLTVVFKLKAPFAPF
LTAFDCTTAPIVPKHIYEGTDYRKNPANAKAIGSGPFKLKEWVRGSHVHLVKHEGYYRPGEPVLDEIIYRVIPDSASRSV
ALEQGTVQLTQWTDVELFEVPRLSKLPHLTMTTKGYEFFAPHTWLEFNTRIAPMNDKRFRQAVMYAIDRKALLARIYFGL
GKVATGPVSSKTKFYEKNVKAYDFSPEKAKALLDEMGLKPGADGKRVTIPYLVPPYGETHQRTSEFIRQSLARVGIDLQL
QGIDVAGWAEKFSNWDFSMTATTVYQFGDPALGVSRSYVSSNIRKGILFSNTCGYSNPEVDRLFEEAAVATSDDKRQEFY
SEVQKIMVEDVPVAWLLEIDYPNFMDKRLKNVVTTAIGVHDTFGTVSFG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=437, Percent_Identity=28.8329519450801, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789966, Length=474, Percent_Identity=25.7383966244726, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1787052, Length=442, Percent_Identity=28.2805429864253, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787551, Length=477, Percent_Identity=22.8511530398323, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789887, Length=437, Percent_Identity=26.0869565217391, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081878, Length=489, Percent_Identity=22.6993865030675, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787495, Length=519, Percent_Identity=22.7360308285164, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789397, Length=457, Percent_Identity=23.4135667396061, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082063, Length=447, Percent_Identity=22.1476510067114, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59124; Mature: 59124

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFTKLGLLAGAAGIGGIPLGITRAVGQTRGGTLNTIIQPEPPILVTALNQQQPTLT
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEEE
LGGKIYESLLRYDFDLKPLPGLAQSWEVSPDKLTYTFKLHPNITFHDGAPLTSEDVVFSI
ECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCCHHHHHHHH
MKVLIENHARARNTFSRVEKAEAPDPLTVVFKLKAPFAPFLTAFDCTTAPIVPKHIYEGT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCHHHHCCCC
DYRKNPANAKAIGSGPFKLKEWVRGSHVHLVKHEGYYRPGEPVLDEIIYRVIPDSASRSV
CCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEE
ALEQGTVQLTQWTDVELFEVPRLSKLPHLTMTTKGYEFFAPHTWLEFNTRIAPMNDKRFR
EEECCEEEEEECCCCEEECCCCCCCCCCEEEEECCEEEECCCEEEEECCEECCCCHHHHH
QAVMYAIDRKALLARIYFGLGKVATGPVSSKTKFYEKNVKAYDFSPEKAKALLDEMGLKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHCCCC
GADGKRVTIPYLVPPYGETHQRTSEFIRQSLARVGIDLQLQGIDVAGWAEKFSNWDFSMT
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECEEEHHHHHHHCCCCEEEE
ATTVYQFGDPALGVSRSYVSSNIRKGILFSNTCGYSNPEVDRLFEEAAVATSDDKRQEFY
EEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
SEVQKIMVEDVPVAWLLEIDYPNFMDKRLKNVVTTAIGVHDTFGTVSFG
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNRREFTKLGLLAGAAGIGGIPLGITRAVGQTRGGTLNTIIQPEPPILVTALNQQQPTLT
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEEE
LGGKIYESLLRYDFDLKPLPGLAQSWEVSPDKLTYTFKLHPNITFHDGAPLTSEDVVFSI
ECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEEECCCCCCCHHHHHHHH
MKVLIENHARARNTFSRVEKAEAPDPLTVVFKLKAPFAPFLTAFDCTTAPIVPKHIYEGT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCHHHHCCCC
DYRKNPANAKAIGSGPFKLKEWVRGSHVHLVKHEGYYRPGEPVLDEIIYRVIPDSASRSV
CCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEE
ALEQGTVQLTQWTDVELFEVPRLSKLPHLTMTTKGYEFFAPHTWLEFNTRIAPMNDKRFR
EEECCEEEEEECCCCEEECCCCCCCCCCEEEEECCEEEECCCEEEEECCEECCCCHHHHH
QAVMYAIDRKALLARIYFGLGKVATGPVSSKTKFYEKNVKAYDFSPEKAKALLDEMGLKP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEECCCHHHHHHHHHHHCCCC
GADGKRVTIPYLVPPYGETHQRTSEFIRQSLARVGIDLQLQGIDVAGWAEKFSNWDFSMT
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECEEEHHHHHHHCCCCEEEE
ATTVYQFGDPALGVSRSYVSSNIRKGILFSNTCGYSNPEVDRLFEEAAVATSDDKRQEFY
EEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
SEVQKIMVEDVPVAWLLEIDYPNFMDKRLKNVVTTAIGVHDTFGTVSFG
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]