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The map label for this gene is ilvX [H]

Identifier: 86750245

GI number: 86750245

Start: 3581229

End: 3582812

Strand: Reverse

Name: ilvX [H]

Synonym: RPB_3134

Alternate gene names: 86750245

Gene position: 3582812-3581229 (Counterclockwise)

Preceding gene: 86750246

Following gene: 86750244

Centisome position: 67.2

GC content: 69.63

Gene sequence:

>1584_bases
ATGGCAGTCGCGGCACCGGCGGCGTCCGCCATGCAATCGATGACGGGAGCAGACCGGCTTTGCGATGCGCTACTGGCGCA
CGAGGTCGATGTCTGCTTCGCCAATCCTGGCACGTCGGAGATGCATTTCGTGGCGGCGCTCGATCGCCAGCCGCGGATGC
GGTGCATCCTGGGTCTGTTCGAGGGCGTCGTCACCGGCGCTGCGGACGGTTACGCCCGGATGCGCGACCGGCCGGCCGCG
ACGCTGCTGCATCTCGGCCCGGGCCTCGCCAACGGCCTAGCCAACATCCACAACGCCCGCCGCGCGCATTCGCCGATGAT
CAACGTCGTCGGCGATCACGCGGTCGAGCATCTGCCGCTGGACTCGCCGCTGACCAGCGACATCGACTCGCTGGCGCGCC
CGATGTCGCGCTGGGTCAAACGGATCGGCGATCCGACGCGGATCGATCTCGACGTGGCGGAGGCCTGGTCGCAGAGCATG
GCGCCCGGGATTTCCACCCTGATCCTGCCGGCGGACATGGCCTGGAACGAGCATGTCTATCGCGCGCCGCCGGCGCGGGG
CAGGCGGCTGCCGCAAGCGCCGCTTCAGCAGGAGACGGTGGCGCGGATCGGCGCCGCGATCCGCACCAAGCGGCGTGTCG
TGCTGCTGCTGACCGGCGCCGCCTTGCGTGAGCAGGCACTGTCGATTGCGGACGGCATCCGCCGCAGCGCCGGTGTCGAG
ATCTACGCGCAGGGCGCCAACGGCCGGATCGAACGTGGCCGCGGCCGCGCGCCGATCGCGAAGCTGGCGATGACCCACGA
GATCGGCGGCCGGATGCTGCAGGACGTCGACATGGTGGTGCTGATCGGCGCCAAGGAGCCGGTGTCGTTCTTCGCCTATC
CGAACAGGCCGGGAAGGCTGGCGCCGGCGAATTGCGAGATCGTCCGGCTGGCGGGGCCGGATCAGGATTTGGTACAGGCG
CTCGAATGGCTGGCCGACGAGGTCGATGCCCCGCGCGCGCCGGTCGCGGTCGCGCCGCGCGCCGCTGCGGCGCCGGTCGC
CAGCGGGGCGTTGACCGTCGATATGGTGAACCGCCTCGTCGCCGCGCGGCTGCCCGAGCAAGCGATCGTCTGCGACGAGG
CACTGACCTCGTCGGGCTTCTTCGACCTGTCTTACGACGCCCCGCCGCACGACTATCTGCAGATCACCGGTGGCGCGATC
GGGATCGGCATTCCGATGGCCGCGGGCGCCGCGGTCGCGTGTCCCGACCGCAAGGTGATCAACCTGCAGGCCGACGGCAG
CGGCATGTACACGGTGCAGGGTCTGTGGACCCACGCGCGCGAGAATCTCGACGTTCTCACCATCGTATTTTCGAACCGCT
CCTACGCCACGCTGTGGGGCGAGATGAGCAAGGTCGGTGCGCAGACGCCGGGACGCAATGCGCAGCGCATGCTGCAGCTC
GATCAGCCCGAGCTCGACTGGACCAAGCTGGCTGCGGGGCTCGGCGTCGAATCCCGCCGAGTCACGACGGCGTCGGAGTT
CTCGCGCGCGTTCGATGCCGCGCTCGGCCGGCGTGGTCCTGCCGTGATCGAGGCAATGGTGTGA

Upstream 100 bases:

>100_bases
CGCACGCGTTCGACGAGCGCATCGATCTCGAAAGCCTGCGCAAGACGACGCTGAGCATCGCGCTGTTCGTCGCGGAATGG
TGTGGGCTGAGGAAGCTGTG

Downstream 100 bases:

>100_bases
TGAGGAGGTCGGTGTCGATGCCATCTTCGACGCCGCCGGCGAACGATCTGGTTCTATTCAACTCAATGCGGGGGATCTCA
TGAACAGACGCGAATTCACC

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAVAAPAASAMQSMTGADRLCDALLAHEVDVCFANPGTSEMHFVAALDRQPRMRCILGLFEGVVTGAADGYARMRDRPAA
TLLHLGPGLANGLANIHNARRAHSPMINVVGDHAVEHLPLDSPLTSDIDSLARPMSRWVKRIGDPTRIDLDVAEAWSQSM
APGISTLILPADMAWNEHVYRAPPARGRRLPQAPLQQETVARIGAAIRTKRRVVLLLTGAALREQALSIADGIRRSAGVE
IYAQGANGRIERGRGRAPIAKLAMTHEIGGRMLQDVDMVVLIGAKEPVSFFAYPNRPGRLAPANCEIVRLAGPDQDLVQA
LEWLADEVDAPRAPVAVAPRAAAAPVASGALTVDMVNRLVAARLPEQAIVCDEALTSSGFFDLSYDAPPHDYLQITGGAI
GIGIPMAAGAAVACPDRKVINLQADGSGMYTVQGLWTHARENLDVLTIVFSNRSYATLWGEMSKVGAQTPGRNAQRMLQL
DQPELDWTKLAAGLGVESRRVTTASEFSRAFDAALGRRGPAVIEAMV

Sequences:

>Translated_527_residues
MAVAAPAASAMQSMTGADRLCDALLAHEVDVCFANPGTSEMHFVAALDRQPRMRCILGLFEGVVTGAADGYARMRDRPAA
TLLHLGPGLANGLANIHNARRAHSPMINVVGDHAVEHLPLDSPLTSDIDSLARPMSRWVKRIGDPTRIDLDVAEAWSQSM
APGISTLILPADMAWNEHVYRAPPARGRRLPQAPLQQETVARIGAAIRTKRRVVLLLTGAALREQALSIADGIRRSAGVE
IYAQGANGRIERGRGRAPIAKLAMTHEIGGRMLQDVDMVVLIGAKEPVSFFAYPNRPGRLAPANCEIVRLAGPDQDLVQA
LEWLADEVDAPRAPVAVAPRAAAAPVASGALTVDMVNRLVAARLPEQAIVCDEALTSSGFFDLSYDAPPHDYLQITGGAI
GIGIPMAAGAAVACPDRKVINLQADGSGMYTVQGLWTHARENLDVLTIVFSNRSYATLWGEMSKVGAQTPGRNAQRMLQL
DQPELDWTKLAAGLGVESRRVTTASEFSRAFDAALGRRGPAVIEAMV
>Mature_526_residues
AVAAPAASAMQSMTGADRLCDALLAHEVDVCFANPGTSEMHFVAALDRQPRMRCILGLFEGVVTGAADGYARMRDRPAAT
LLHLGPGLANGLANIHNARRAHSPMINVVGDHAVEHLPLDSPLTSDIDSLARPMSRWVKRIGDPTRIDLDVAEAWSQSMA
PGISTLILPADMAWNEHVYRAPPARGRRLPQAPLQQETVARIGAAIRTKRRVVLLLTGAALREQALSIADGIRRSAGVEI
YAQGANGRIERGRGRAPIAKLAMTHEIGGRMLQDVDMVVLIGAKEPVSFFAYPNRPGRLAPANCEIVRLAGPDQDLVQAL
EWLADEVDAPRAPVAVAPRAAAAPVASGALTVDMVNRLVAARLPEQAIVCDEALTSSGFFDLSYDAPPHDYLQITGGAIG
IGIPMAAGAAVACPDRKVINLQADGSGMYTVQGLWTHARENLDVLTIVFSNRSYATLWGEMSKVGAQTPGRNAQRMLQLD
QPELDWTKLAAGLGVESRRVTTASEFSRAFDAALGRRGPAVIEAMV

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=23.5714285714286, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56249; Mature: 56118

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAAPAASAMQSMTGADRLCDALLAHEVDVCFANPGTSEMHFVAALDRQPRMRCILGLF
CCCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHH
EGVVTGAADGYARMRDRPAATLLHLGPGLANGLANIHNARRAHSPMINVVGDHAVEHLPL
HHHHHCCCCCHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DSPLTSDIDSLARPMSRWVKRIGDPTRIDLDVAEAWSQSMAPGISTLILPADMAWNEHVY
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
RAPPARGRRLPQAPLQQETVARIGAAIRTKRRVVLLLTGAALREQALSIADGIRRSAGVE
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCE
IYAQGANGRIERGRGRAPIAKLAMTHEIGGRMLQDVDMVVLIGAKEPVSFFAYPNRPGRL
EEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCC
APANCEIVRLAGPDQDLVQALEWLADEVDAPRAPVAVAPRAAAAPVASGALTVDMVNRLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
AARLPEQAIVCDEALTSSGFFDLSYDAPPHDYLQITGGAIGIGIPMAAGAAVACPDRKVI
HHHCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCEEEECCHHHCCCEEECCCCEEE
NLQADGSGMYTVQGLWTHARENLDVLTIVFSNRSYATLWGEMSKVGAQTPGRNAQRMLQL
EEEECCCCEEEEHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCHHHHHHC
DQPELDWTKLAAGLGVESRRVTTASEFSRAFDAALGRRGPAVIEAMV
CCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECC
>Mature Secondary Structure 
AVAAPAASAMQSMTGADRLCDALLAHEVDVCFANPGTSEMHFVAALDRQPRMRCILGLF
CCCCCHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHH
EGVVTGAADGYARMRDRPAATLLHLGPGLANGLANIHNARRAHSPMINVVGDHAVEHLPL
HHHHHCCCCCHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DSPLTSDIDSLARPMSRWVKRIGDPTRIDLDVAEAWSQSMAPGISTLILPADMAWNEHVY
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
RAPPARGRRLPQAPLQQETVARIGAAIRTKRRVVLLLTGAALREQALSIADGIRRSAGVE
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCE
IYAQGANGRIERGRGRAPIAKLAMTHEIGGRMLQDVDMVVLIGAKEPVSFFAYPNRPGRL
EEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCC
APANCEIVRLAGPDQDLVQALEWLADEVDAPRAPVAVAPRAAAAPVASGALTVDMVNRLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
AARLPEQAIVCDEALTSSGFFDLSYDAPPHDYLQITGGAIGIGIPMAAGAAVACPDRKVI
HHHCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCEEEECCHHHCCCEEECCCCEEE
NLQADGSGMYTVQGLWTHARENLDVLTIVFSNRSYATLWGEMSKVGAQTPGRNAQRMLQL
EEEECCCCEEEEHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCHHHHHHC
DQPELDWTKLAAGLGVESRRVTTASEFSRAFDAALGRRGPAVIEAMV
CCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]