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The map label for this gene is holB [H]

Identifier: 86749797

GI number: 86749797

Start: 3061833

End: 3062873

Strand: Direct

Name: holB [H]

Synonym: RPB_2680

Alternate gene names: 86749797

Gene position: 3061833-3062873 (Clockwise)

Preceding gene: 86749796

Following gene: 86749798

Centisome position: 57.43

GC content: 70.61

Gene sequence:

>1041_bases
ATGAGCGCGCGCAAGGCTTCCACCGAGATCGCCGCGAAATCGCCGCGCGAGACCACGGCGCTGTTCGGGCATGCCGCCGC
CGAGCAGGCGCTGCTCGATGCCTATCGCAGCGGCCGCATCGCGCACGCCTGGCTGATCGGCGGCCCGCAGGGCATCGGCA
AGGCGACGCTGGCCTACCGGATGGCGCGCTTCGTGCTGGCGCATCGCGATCCGATGGCGGATGCGGTGCAGGGCGCCGCG
ACCCTCGACCTCGACCCGTCGCATCCGGTGGTGCGCCACGTCGCCGCCGAGGCCCATGGCGGCCTGCTGACGCTGGAGCG
CTCGGTCAACGACAAGGGCGTGCTGCGCAACGTCATCACCGTCGACGAGTCGCGCGAGACGATCGGCTTCTTCGGTTCGA
CCGCGGCGGTCGAAGGCTGGCGGGTGTGCATCGTCGATACCGTCGACGATCTCAACGCCTCGTCCGCCAACGCGCTGCTC
AAGGTGATCGAGGAGCCGCCGCAGCGCTCGCTGTTTCTGCTGATCAGCAATTCGCCGGCGCGGCTGCTGCCGACGATCCT
GTCGCGCTGCCGCAAGCTGATGCTGCGGCCGTTATCCGTCAGCGACGTGATCGCCGCGGCGCTCGAGGCCACCGATCTTT
CGCCCGGCGATGCCAAGCTCGCCGAGGCCGCCGCGGCCTCGGAAGGCAGCGTGGCGCGGGCGCTAACGCTGCTCGGTGGC
GACGCGCTGACGCTGCAGCAGAAAACCACCGCGCTGCTCGACAGCCTGCCGAACGTCGATCCGCGCGCGCTGCATGCGCT
GGGCGAGGCGATCGGCGGCACCGACCGCACCACCCTGGCGGCGTTCGTCGACGGCGTCGACCGCTGGATCGCCGGCAGGC
TGCGGACAGGCGATGCCAACGCGGAACTGCCGCGCCTTGCACGGCTGGCGGAGGTGTGGGAAAAGATCGGCCGCGCCGCG
CGCGACACCGAAGCCTATAATCTGGAGCGCAAGCCGCTGGTTTTCTCGGTGTTCGGGCTCCTCGCGGAGGCGGTGCGCTG
A

Upstream 100 bases:

>100_bases
TGACGGTGCAGGAGCGGCTGCTGCAGTGGCCGCAGGCCAAACCCGCGGTGACGCCGAAGGCGAAAGCTAAGACGAAAACG
AAGACCAAGGCGCGATCGGC

Downstream 100 bases:

>100_bases
TGCGCTCGGGCGATCACACAGGCTGATGGTCAGCGCCGCCGCCCTCATTCGATCCAGTTGTTGAAAGGCGTCCGCGCAGA
TGGCGCAGCGAAACTCGTTC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSARKASTEIAAKSPRETTALFGHAAAEQALLDAYRSGRIAHAWLIGGPQGIGKATLAYRMARFVLAHRDPMADAVQGAA
TLDLDPSHPVVRHVAAEAHGGLLTLERSVNDKGVLRNVITVDESRETIGFFGSTAAVEGWRVCIVDTVDDLNASSANALL
KVIEEPPQRSLFLLISNSPARLLPTILSRCRKLMLRPLSVSDVIAAALEATDLSPGDAKLAEAAAASEGSVARALTLLGG
DALTLQQKTTALLDSLPNVDPRALHALGEAIGGTDRTTLAAFVDGVDRWIAGRLRTGDANAELPRLARLAEVWEKIGRAA
RDTEAYNLERKPLVFSVFGLLAEAVR

Sequences:

>Translated_346_residues
MSARKASTEIAAKSPRETTALFGHAAAEQALLDAYRSGRIAHAWLIGGPQGIGKATLAYRMARFVLAHRDPMADAVQGAA
TLDLDPSHPVVRHVAAEAHGGLLTLERSVNDKGVLRNVITVDESRETIGFFGSTAAVEGWRVCIVDTVDDLNASSANALL
KVIEEPPQRSLFLLISNSPARLLPTILSRCRKLMLRPLSVSDVIAAALEATDLSPGDAKLAEAAAASEGSVARALTLLGG
DALTLQQKTTALLDSLPNVDPRALHALGEAIGGTDRTTLAAFVDGVDRWIAGRLRTGDANAELPRLARLAEVWEKIGRAA
RDTEAYNLERKPLVFSVFGLLAEAVR
>Mature_345_residues
SARKASTEIAAKSPRETTALFGHAAAEQALLDAYRSGRIAHAWLIGGPQGIGKATLAYRMARFVLAHRDPMADAVQGAAT
LDLDPSHPVVRHVAAEAHGGLLTLERSVNDKGVLRNVITVDESRETIGFFGSTAAVEGWRVCIVDTVDDLNASSANALLK
VIEEPPQRSLFLLISNSPARLLPTILSRCRKLMLRPLSVSDVIAAALEATDLSPGDAKLAEAAAASEGSVARALTLLGGD
ALTLQQKTTALLDSLPNVDPRALHALGEAIGGTDRTTLAAFVDGVDRWIAGRLRTGDANAELPRLARLAEVWEKIGRAAR
DTEAYNLERKPLVFSVFGLLAEAVR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=250, Percent_Identity=26.8, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787341, Length=237, Percent_Identity=29.957805907173, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36739; Mature: 36608

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARKASTEIAAKSPRETTALFGHAAAEQALLDAYRSGRIAHAWLIGGPQGIGKATLAYR
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHH
MARFVLAHRDPMADAVQGAATLDLDPSHPVVRHVAAEAHGGLLTLERSVNDKGVLRNVIT
HHHHHHHCCCCHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHE
VDESRETIGFFGSTAAVEGWRVCIVDTVDDLNASSANALLKVIEEPPQRSLFLLISNSPA
ECCCCCHHHCCCCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
RLLPTILSRCRKLMLRPLSVSDVIAAALEATDLSPGDAKLAEAAAASEGSVARALTLLGG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCC
DALTLQQKTTALLDSLPNVDPRALHALGEAIGGTDRTTLAAFVDGVDRWIAGRLRTGDAN
CEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
AELPRLARLAEVWEKIGRAARDTEAYNLERKPLVFSVFGLLAEAVR
CCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SARKASTEIAAKSPRETTALFGHAAAEQALLDAYRSGRIAHAWLIGGPQGIGKATLAYR
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHH
MARFVLAHRDPMADAVQGAATLDLDPSHPVVRHVAAEAHGGLLTLERSVNDKGVLRNVIT
HHHHHHHCCCCHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHE
VDESRETIGFFGSTAAVEGWRVCIVDTVDDLNASSANALLKVIEEPPQRSLFLLISNSPA
ECCCCCHHHCCCCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
RLLPTILSRCRKLMLRPLSVSDVIAAALEATDLSPGDAKLAEAAAASEGSVARALTLLGG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCC
DALTLQQKTTALLDSLPNVDPRALHALGEAIGGTDRTTLAAFVDGVDRWIAGRLRTGDAN
CEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
AELPRLARLAEVWEKIGRAARDTEAYNLERKPLVFSVFGLLAEAVR
CCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]