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The map label for this gene is metG [H]

Identifier: 86749798

GI number: 86749798

Start: 3062953

End: 3064497

Strand: Direct

Name: metG [H]

Synonym: RPB_2681

Alternate gene names: 86749798

Gene position: 3062953-3064497 (Clockwise)

Preceding gene: 86749797

Following gene: 86749799

Centisome position: 57.45

GC content: 65.7

Gene sequence:

>1545_bases
ATGGCGCAGCGAAACTCGTTCTACATCACCACCGCAATCTCCTATCCCAACGGCGCGCCGCATATCGGCCACGCCTATGA
GGCGATCGCGACCGACGCGCTGGCGCGATTCCAGCGGCTCGACGGCCGTGACGTGTTCTTCCTGACCGGCACCGACGAGC
ACGGCCTGAAGATGATCCAGACCGCGCAGAAGGAGGGCGTCGAGACCCACGTGCTCGCCGCCCGCAATGCCGGCCGCTTC
CGCGACATGGACGACCGCTTCGGCATCTCCTACGACCGCTTCATCCGCACCTCGGAAGAGCAGCATCATCGCTCCGTCCA
GGAGGTCTGGAAGCGGATGCAGGCGAACGGCGACATCTATCTCGACAGCTATGCCGGCTGGTATTCGGTCCGCGACGAGG
CCTATTACGCCGAGGACGAAACCGTCGTCGGCGAGGATCAGATTCGCCGCGGCCCGCAGGGCACGCCGGTCGAATGGGTC
GAGGAGCGGAGCTACTTCTTCAAGCTCTCGGCCTATCAGGACAAACTCCTGAAGCTGTACGAGGACCAGCCGGACTTCAT
CGGCCCGGATTCCCGCCGCAACGAGGTGATCAGCTTCGTCAAGGGCGGGCTGAAGGACCTGTCGGTGTCGCGCACCACCT
TCGACTGGGGCGTCAAGGTGCCGGGCGATCCGGAGCACGTGATGTATGTCTGGGTCGATGCGCTGACCAACTACATCACC
GGCGTCGGCTTCCCCGACGAGAGCGACGCCAACTGGCATTATTGGCCGGCCGACGTGCACGTCATCGGCAAGGACATCAT
CCGCTTCCACGCGGTGTACTGGCCGGCGTTCCTGATGTCGGCCGGCATCCCGATCCAGAAGCGCGTCTACGCCCACGGCT
TTTTGTTCAACAAGGGCGAGAAGATGTCGAAATCGGTCGGCAATATCGTCGACCCGTTCAATCTCGCCGATCAATACGGC
GTCGATCAGGTGCGCTACTTCTTCCTGCGCGAGGTGCCGTTCGGCGCCGACGGCAGCTATAATCACGAGGCGATCGTCAA
TCGCATCAACGCCGACCTCGCCAACGACCTCGGCAATCTGGCGCAGCGCTCGCTGACCATGGTGGCCAAGCAGTGCGACG
CCCAGGTGCCGCAGCACGGCGAGTTCAGCGAGGCCGACAAGGCGATGCTGGCGATGGCCGACGGCATGATCGCGATCGCA
CGCCCGGCGATGGCAACGCAGCAGATCCATCAGGCGCTGAACGCGGTGTGGGCGGTGGTGGCGGAAGCCAACCGTTACTT
CGCCGGCGAAGCGCCGTGGGCGCTGGCCAAGACCGATCCGGCGCGGCAGAAGACGGTGCTCTACGTCACCGCCGAAGTGC
TGCGGCAGGTCGGCATCCTGGCGCAGCCCGCGATCCCGGCCTCGGCGGCCAAGCTGCTCGACATCCTCGGCGTCGCCGAG
GGCGCGCGCGACTTCGCGGCGCTGCCGACGCGGATCGTGCCGGGCACGCCACTGCCGGCCCCGGCGCCTATCTTCCCGCG
CTATGTCGAGCCGGCGACCGCCTGA

Upstream 100 bases:

>100_bases
CTCGCGGAGGCGGTGCGCTGATGCGCTCGGGCGATCACACAGGCTGATGGTCAGCGCCGCCGCCCTCATTCGATCCAGTT
GTTGAAAGGCGTCCGCGCAG

Downstream 100 bases:

>100_bases
TGCCGATGCTGGTCGACAGCCACTGCCATCTCGACTTCCCCGACTTTGCCGACGATCTCGCCGGCATCGTCGCCCGCGCC
GAAGCGAGCGGGGTGGGGCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MAQRNSFYITTAISYPNGAPHIGHAYEAIATDALARFQRLDGRDVFFLTGTDEHGLKMIQTAQKEGVETHVLAARNAGRF
RDMDDRFGISYDRFIRTSEEQHHRSVQEVWKRMQANGDIYLDSYAGWYSVRDEAYYAEDETVVGEDQIRRGPQGTPVEWV
EERSYFFKLSAYQDKLLKLYEDQPDFIGPDSRRNEVISFVKGGLKDLSVSRTTFDWGVKVPGDPEHVMYVWVDALTNYIT
GVGFPDESDANWHYWPADVHVIGKDIIRFHAVYWPAFLMSAGIPIQKRVYAHGFLFNKGEKMSKSVGNIVDPFNLADQYG
VDQVRYFFLREVPFGADGSYNHEAIVNRINADLANDLGNLAQRSLTMVAKQCDAQVPQHGEFSEADKAMLAMADGMIAIA
RPAMATQQIHQALNAVWAVVAEANRYFAGEAPWALAKTDPARQKTVLYVTAEVLRQVGILAQPAIPASAAKLLDILGVAE
GARDFAALPTRIVPGTPLPAPAPIFPRYVEPATA

Sequences:

>Translated_514_residues
MAQRNSFYITTAISYPNGAPHIGHAYEAIATDALARFQRLDGRDVFFLTGTDEHGLKMIQTAQKEGVETHVLAARNAGRF
RDMDDRFGISYDRFIRTSEEQHHRSVQEVWKRMQANGDIYLDSYAGWYSVRDEAYYAEDETVVGEDQIRRGPQGTPVEWV
EERSYFFKLSAYQDKLLKLYEDQPDFIGPDSRRNEVISFVKGGLKDLSVSRTTFDWGVKVPGDPEHVMYVWVDALTNYIT
GVGFPDESDANWHYWPADVHVIGKDIIRFHAVYWPAFLMSAGIPIQKRVYAHGFLFNKGEKMSKSVGNIVDPFNLADQYG
VDQVRYFFLREVPFGADGSYNHEAIVNRINADLANDLGNLAQRSLTMVAKQCDAQVPQHGEFSEADKAMLAMADGMIAIA
RPAMATQQIHQALNAVWAVVAEANRYFAGEAPWALAKTDPARQKTVLYVTAEVLRQVGILAQPAIPASAAKLLDILGVAE
GARDFAALPTRIVPGTPLPAPAPIFPRYVEPATA
>Mature_513_residues
AQRNSFYITTAISYPNGAPHIGHAYEAIATDALARFQRLDGRDVFFLTGTDEHGLKMIQTAQKEGVETHVLAARNAGRFR
DMDDRFGISYDRFIRTSEEQHHRSVQEVWKRMQANGDIYLDSYAGWYSVRDEAYYAEDETVVGEDQIRRGPQGTPVEWVE
ERSYFFKLSAYQDKLLKLYEDQPDFIGPDSRRNEVISFVKGGLKDLSVSRTTFDWGVKVPGDPEHVMYVWVDALTNYITG
VGFPDESDANWHYWPADVHVIGKDIIRFHAVYWPAFLMSAGIPIQKRVYAHGFLFNKGEKMSKSVGNIVDPFNLADQYGV
DQVRYFFLREVPFGADGSYNHEAIVNRINADLANDLGNLAQRSLTMVAKQCDAQVPQHGEFSEADKAMLAMADGMIAIAR
PAMATQQIHQALNAVWAVVAEANRYFAGEAPWALAKTDPARQKTVLYVTAEVLRQVGILAQPAIPASAAKLLDILGVAEG
ARDFAALPTRIVPGTPLPAPAPIFPRYVEPATA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=37.4269005847953, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI14043022, Length=515, Percent_Identity=23.1067961165049, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI7661872, Length=192, Percent_Identity=26.0416666666667, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788432, Length=498, Percent_Identity=24.6987951807229, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1786861, Length=179, Percent_Identity=26.2569832402235, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212645096, Length=499, Percent_Identity=33.8677354709419, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI71992781, Length=386, Percent_Identity=33.419689119171, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17540794, Length=559, Percent_Identity=25.2236135957066, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321610, Length=522, Percent_Identity=32.3754789272031, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321704, Length=519, Percent_Identity=23.8921001926782, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21358483, Length=504, Percent_Identity=35.9126984126984, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24655311, Length=551, Percent_Identity=25.0453720508167, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355409, Length=186, Percent_Identity=27.4193548387097, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 57461; Mature: 57330

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQRNSFYITTAISYPNGAPHIGHAYEAIATDALARFQRLDGRDVFFLTGTDEHGLKMIQ
CCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TAQKEGVETHVLAARNAGRFRDMDDRFGISYDRFIRTSEEQHHRSVQEVWKRMQANGDIY
HHHHCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
LDSYAGWYSVRDEAYYAEDETVVGEDQIRRGPQGTPVEWVEERSYFFKLSAYQDKLLKLY
EECCCCEEEECCCEEECCCCCEECHHHHCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHH
EDQPDFIGPDSRRNEVISFVKGGLKDLSVSRTTFDWGVKVPGDPEHVMYVWVDALTNYIT
CCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHH
GVGFPDESDANWHYWPADVHVIGKDIIRFHAVYWPAFLMSAGIPIQKRVYAHGFLFNKGE
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEECCCH
KMSKSVGNIVDPFNLADQYGVDQVRYFFLREVPFGADGSYNHEAIVNRINADLANDLGNL
HHHHHHCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AQRSLTMVAKQCDAQVPQHGEFSEADKAMLAMADGMIAIARPAMATQQIHQALNAVWAVV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHH
AEANRYFAGEAPWALAKTDPARQKTVLYVTAEVLRQVGILAQPAIPASAAKLLDILGVAE
HHHCCEECCCCCEEECCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
GARDFAALPTRIVPGTPLPAPAPIFPRYVEPATA
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQRNSFYITTAISYPNGAPHIGHAYEAIATDALARFQRLDGRDVFFLTGTDEHGLKMIQ
CCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
TAQKEGVETHVLAARNAGRFRDMDDRFGISYDRFIRTSEEQHHRSVQEVWKRMQANGDIY
HHHHCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCEE
LDSYAGWYSVRDEAYYAEDETVVGEDQIRRGPQGTPVEWVEERSYFFKLSAYQDKLLKLY
EECCCCEEEECCCEEECCCCCEECHHHHCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHH
EDQPDFIGPDSRRNEVISFVKGGLKDLSVSRTTFDWGVKVPGDPEHVMYVWVDALTNYIT
CCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHH
GVGFPDESDANWHYWPADVHVIGKDIIRFHAVYWPAFLMSAGIPIQKRVYAHGFLFNKGE
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEECCCH
KMSKSVGNIVDPFNLADQYGVDQVRYFFLREVPFGADGSYNHEAIVNRINADLANDLGNL
HHHHHHCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AQRSLTMVAKQCDAQVPQHGEFSEADKAMLAMADGMIAIARPAMATQQIHQALNAVWAVV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHH
AEANRYFAGEAPWALAKTDPARQKTVLYVTAEVLRQVGILAQPAIPASAAKLLDILGVAE
HHHCCEECCCCCEEECCCCCCCCCEEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
GARDFAALPTRIVPGTPLPAPAPIFPRYVEPATA
CCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]