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The map label for this gene is ribE [H]

Identifier: 86749754

GI number: 86749754

Start: 3017669

End: 3018277

Strand: Direct

Name: ribE [H]

Synonym: RPB_2637

Alternate gene names: 86749754

Gene position: 3017669-3018277 (Clockwise)

Preceding gene: 86749753

Following gene: 162138521

Centisome position: 56.6

GC content: 67.49

Gene sequence:

>609_bases
ATGTTCACCGGCATTGTCACCGACATCGGCGAAATCGAAGCCCTGACGCCCACGGCGCAGGGACAACTGCACCGGCTGCG
GATCGGCTGCAGCTACGATCAGGCCACGATCGCCGACGGCGCCTCGATCGCCTCGAACGGCATCTGCATGACCGTGGTCG
GGTCGGGCACAGCGGGCGGCCGCACCTGGTACGAGGTCGACGCCGGCGCCGAGACCCTGGCGCTGACCACCGCGAAGCAC
TGGGGCGTCGGCACCAGGCTCAATCTCGAGCGCGCGCTGAAGATCGGCGACGAGCTCGGCGGTCACATCGTCACCGGCCA
CGTTGACGGCATCGCCACCGTGGTGGCGCGCGAGGACCTGCCGGAGATGGCACGGTTCACCCTCGAGACGACGCGCGAGC
TGGCGCGCTTCATTGCTCCGAAGGGCTCGGTAACGCTCGACGGCACCTCGCTGACGGTGAACACCGTCGAGGGCACGCGT
TTCTCGGTGCTGATCATCCCGCACACGCTGAGCGCCACCATCCTCGGTGGCTGGCGGGTCGACAGCGAAGTCAACATCGA
GGTCGACCTGATGGCGCGCTATGCCGCGCGGCTGAGCGAGATGAAATAG

Upstream 100 bases:

>100_bases
CGCCTCCATCACCGCGTCGTCGGCTTGGCGCGTCCGCGACAGCGAACGCCTCGGACCGGACACGCTCACGATTTTCGAAC
CGGCTTAAGGTCTGCGTCTC

Downstream 100 bases:

>100_bases
GCGGCAGCACAAACGAAGTGGTCACGCGCGGGCTTGACCCGCGCATCCATCCTCTTAACAAGAACGCATCCAAATTCTCT
CGACGACGATGGATCGCCGG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MFTGIVTDIGEIEALTPTAQGQLHRLRIGCSYDQATIADGASIASNGICMTVVGSGTAGGRTWYEVDAGAETLALTTAKH
WGVGTRLNLERALKIGDELGGHIVTGHVDGIATVVAREDLPEMARFTLETTRELARFIAPKGSVTLDGTSLTVNTVEGTR
FSVLIIPHTLSATILGGWRVDSEVNIEVDLMARYAARLSEMK

Sequences:

>Translated_202_residues
MFTGIVTDIGEIEALTPTAQGQLHRLRIGCSYDQATIADGASIASNGICMTVVGSGTAGGRTWYEVDAGAETLALTTAKH
WGVGTRLNLERALKIGDELGGHIVTGHVDGIATVVAREDLPEMARFTLETTRELARFIAPKGSVTLDGTSLTVNTVEGTR
FSVLIIPHTLSATILGGWRVDSEVNIEVDLMARYAARLSEMK
>Mature_202_residues
MFTGIVTDIGEIEALTPTAQGQLHRLRIGCSYDQATIADGASIASNGICMTVVGSGTAGGRTWYEVDAGAETLALTTAKH
WGVGTRLNLERALKIGDELGGHIVTGHVDGIATVVAREDLPEMARFTLETTRELARFIAPKGSVTLDGTSLTVNTVEGTR
FSVLIIPHTLSATILGGWRVDSEVNIEVDLMARYAARLSEMK

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=192, Percent_Identity=34.375, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319733, Length=210, Percent_Identity=35.2380952380952, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 21470; Mature: 21470

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVTDIGEIEALTPTAQGQLHRLRIGCSYDQATIADGASIASNGICMTVVGSGTAGG
CCCCEECCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
RTWYEVDAGAETLALTTAKHWGVGTRLNLERALKIGDELGGHIVTGHVDGIATVVAREDL
CEEEEECCCCCEEEEEEHHHCCCCCEECHHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
PEMARFTLETTRELARFIAPKGSVTLDGTSLTVNTVEGTRFSVLIIPHTLSATILGGWRV
HHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCEEEEEEECCCCEEEEEECEEE
DSEVNIEVDLMARYAARLSEMK
CCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTGIVTDIGEIEALTPTAQGQLHRLRIGCSYDQATIADGASIASNGICMTVVGSGTAGG
CCCCEECCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
RTWYEVDAGAETLALTTAKHWGVGTRLNLERALKIGDELGGHIVTGHVDGIATVVAREDL
CEEEEECCCCCEEEEEEHHHCCCCCEECHHHHHHHHHHCCCEEEEECCCCEEEEEECCCC
PEMARFTLETTRELARFIAPKGSVTLDGTSLTVNTVEGTRFSVLIIPHTLSATILGGWRV
HHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCEEEEEEECCCCEEEEEECEEE
DSEVNIEVDLMARYAARLSEMK
CCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]