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The map label for this gene is ribH [H]

Identifier: 162138521

GI number: 162138521

Start: 3018417

End: 3018908

Strand: Direct

Name: ribH [H]

Synonym: RPB_2638

Alternate gene names: 162138521

Gene position: 3018417-3018908 (Clockwise)

Preceding gene: 86749754

Following gene: 86749756

Centisome position: 56.61

GC content: 69.51

Gene sequence:

>492_bases
ATGGCAGACGCGCGGCGCGCACCCCTGAAAGACCAGACCGACGTTTCGGGCGCGCGCGTCCTGATCGTCGAGGCGCGGTT
CTACGACGATATCCAGGACGCGCTGCTGGACGGCGCAATCGCCGAACTGAAAGCAGCCGGCGCGAGCCACGACGTGCTGA
CCGTGCCCGGCGCGCTGGAAATCCCCGCCGCGGTGGCGATCGCGATCGACGCCGCCGAGGCCGCCGGCAGGCCCTATGAT
GCGGCAATCGCGCTCGGCTGCGTGGTGCGGGGCGAGACCATCCATTTCGAGATCGTCTCGATGGAGTCGGCGCGCGCCCT
GATGGACCTGTCGGTGGCGCGGAAATTCCCGCTCGGCAACGGCATCATCACCGTCAACACCGACGAACAGGCCTGGGCGC
GAGCAAAGCCCGACGATCTCAACAAGGGCGGTGACGCCGCGCGCGCCGCGCTGGCGATGCTGCGGATCAAGCGCCGACTG
GCGCGGGGCTGA

Upstream 100 bases:

>100_bases
CGCGCATCCATCCTCTTAACAAGAACGCATCCAAATTCTCTCGACGACGATGGATCGCCGGGTCGAGCCCGGCAATGACG
ACCAAAGGCAAGGCACCAGA

Downstream 100 bases:

>100_bases
CGATGGCCGATTCCAAAAAGCCGGCGATCAAGAAGCCGGTCCCGAAGGGCGACCGCAAGGCCAATCGGCGCGGCGCTGCG
CGGCTCGCGGCCGTGCAGGC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase 1; Lumazine synthase 1; Riboflavin synthase 1 beta chain [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MADARRAPLKDQTDVSGARVLIVEARFYDDIQDALLDGAIAELKAAGASHDVLTVPGALEIPAAVAIAIDAAEAAGRPYD
AAIALGCVVRGETIHFEIVSMESARALMDLSVARKFPLGNGIITVNTDEQAWARAKPDDLNKGGDAARAALAMLRIKRRL
ARG

Sequences:

>Translated_163_residues
MADARRAPLKDQTDVSGARVLIVEARFYDDIQDALLDGAIAELKAAGASHDVLTVPGALEIPAAVAIAIDAAEAAGRPYD
AAIALGCVVRGETIHFEIVSMESARALMDLSVARKFPLGNGIITVNTDEQAWARAKPDDLNKGGDAARAALAMLRIKRRL
ARG
>Mature_162_residues
ADARRAPLKDQTDVSGARVLIVEARFYDDIQDALLDGAIAELKAAGASHDVLTVPGALEIPAAVAIAIDAAEAAGRPYDA
AIALGCVVRGETIHFEIVSMESARALMDLSVARKFPLGNGIITVNTDEQAWARAKPDDLNKGGDAARAALAMLRIKRRLA
RG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=147, Percent_Identity=40.8163265306122, Blast_Score=89, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324429, Length=151, Percent_Identity=37.0860927152318, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 17158; Mature: 17027

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADARRAPLKDQTDVSGARVLIVEARFYDDIQDALLDGAIAELKAAGASHDVLTVPGALE
CCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
IPAAVAIAIDAAEAAGRPYDAAIALGCVVRGETIHFEIVSMESARALMDLSVARKFPLGN
CCCEEEEEEECHHHCCCCCHHHEEEEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCCCC
GIITVNTDEQAWARAKPDDLNKGGDAARAALAMLRIKRRLARG
CEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADARRAPLKDQTDVSGARVLIVEARFYDDIQDALLDGAIAELKAAGASHDVLTVPGALE
CCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCC
IPAAVAIAIDAAEAAGRPYDAAIALGCVVRGETIHFEIVSMESARALMDLSVARKFPLGN
CCCEEEEEEECHHHCCCCCHHHEEEEEEEECCEEEEEEEECCHHHHHHHHHHHHCCCCCC
GIITVNTDEQAWARAKPDDLNKGGDAARAALAMLRIKRRLARG
CEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA